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MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells.

Gupta K, Tölzer C, Sari-Ak D, Fitzgerald DJ, Schaffitzel C, Berger I.

Viruses. 2019 Feb 26;11(3). pii: E198. doi: 10.3390/v11030198. Review.


Cloning, expression, and purification of intact polyketide synthase modules.

Maschio L, Parnell AE, Lees NR, Willis CL, Schaffitzel C, Stach JEM, Race PR.

Methods Enzymol. 2019;617:63-82. doi: 10.1016/bs.mie.2018.12.018. Epub 2019 Feb 4.


Efficient production of a mature and functional gamma secretase protease.

Khan I, Krishnaswamy S, Sabale M, Groth D, Wijaya L, Morici M, Berger I, Schaffitzel C, Fraser PE, Martins RN, Verdile G.

Sci Rep. 2018 Aug 27;8(1):12834. doi: 10.1038/s41598-018-30788-w.


New insights into the interplay between the translation machinery and nonsense-mediated mRNA decay factors.

Raimondeau E, Bufton JC, Schaffitzel C.

Biochem Soc Trans. 2018 Jun 19;46(3):503-512. doi: 10.1042/BST20170427. Epub 2018 Apr 6. Review.


Structure of a human cap-dependent 48S translation pre-initiation complex.

Eliseev B, Yeramala L, Leitner A, Karuppasamy M, Raimondeau E, Huard K, Alkalaeva E, Aebersold R, Schaffitzel C.

Nucleic Acids Res. 2018 Mar 16;46(5):2678-2689. doi: 10.1093/nar/gky054.


Cryo-EM structure of Saccharomyces cerevisiae target of rapamycin complex 2.

Karuppasamy M, Kusmider B, Oliveira TM, Gaubitz C, Prouteau M, Loewith R, Schaffitzel C.

Nat Commun. 2017 Nov 23;8(1):1729. doi: 10.1038/s41467-017-01862-0.


Dual function of UPF3B in early and late translation termination.

Neu-Yilik G, Raimondeau E, Eliseev B, Yeramala L, Amthor B, Deniaud A, Huard K, Kerschgens K, Hentze MW, Schaffitzel C, Kulozik AE.

EMBO J. 2017 Oct 16;36(20):2968-2986. doi: 10.15252/embj.201797079. Epub 2017 Sep 12.


Multiprotein Complex Production in E. coli: The SecYEG-SecDFYajC-YidC Holotranslocon.

Berger I, Jiang Q, Schulze RJ, Collinson I, Schaffitzel C.

Methods Mol Biol. 2017;1586:279-290. doi: 10.1007/978-1-4939-6887-9_18.


A central cavity within the holo-translocon suggests a mechanism for membrane protein insertion.

Botte M, Zaccai NR, Nijeholt JL, Martin R, Knoops K, Papai G, Zou J, Deniaud A, Karuppasamy M, Jiang Q, Roy AS, Schulten K, Schultz P, Rappsilber J, Zaccai G, Berger I, Collinson I, Schaffitzel C.

Sci Rep. 2016 Dec 7;6:38399. doi: 10.1038/srep38399.


MCM2-7 conformational changes in the presence of DNA.

Schaffitzel C.

Cell Cycle. 2016 Sep 16;15(18):2391-2. doi: 10.1080/15384101.2016.1214046. Epub 2016 Aug 2. No abstract available.


Membrane protein insertion and assembly by the bacterial holo-translocon SecYEG-SecDF-YajC-YidC.

Komar J, Alvira S, Schulze RJ, Martin R, Lycklama A Nijeholt JA, Lee SC, Dafforn TR, Deckers-Hebestreit G, Berger I, Schaffitzel C, Collinson I.

Biochem J. 2016 Oct 1;473(19):3341-54. doi: 10.1042/BCJ20160545. Epub 2016 Jul 19.


PABP enhances release factor recruitment and stop codon recognition during translation termination.

Ivanov A, Mikhailova T, Eliseev B, Yeramala L, Sokolova E, Susorov D, Shuvalov A, Schaffitzel C, Alkalaeva E.

Nucleic Acids Res. 2016 Sep 19;44(16):7766-76. doi: 10.1093/nar/gkw635. Epub 2016 Jul 14.


Cell-Free Synthesis of Macromolecular Complexes.

Botte M, Deniaud A, Schaffitzel C.

Adv Exp Med Biol. 2016;896:79-95. doi: 10.1007/978-3-319-27216-0_6. Review.


ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts.

Nie Y, Chaillet M, Becke C, Haffke M, Pelosse M, Fitzgerald D, Collinson I, Schaffitzel C, Berger I.

Adv Exp Med Biol. 2016;896:27-42. doi: 10.1007/978-3-319-27216-0_3. Review.


The Nucleosome Remodeling and Deacetylase Complex NuRD Is Built from Preformed Catalytically Active Sub-modules.

Zhang W, Aubert A, Gomez de Segura JM, Karuppasamy M, Basu S, Murthy AS, Diamante A, Drury TA, Balmer J, Cramard J, Watson AA, Lando D, Lee SF, Palayret M, Kloet SL, Smits AH, Deery MJ, Vermeulen M, Hendrich B, Klenerman D, Schaffitzel C, Berger I, Laue ED.

J Mol Biol. 2016 Jul 17;428(14):2931-42. doi: 10.1016/j.jmb.2016.04.025. Epub 2016 Apr 23.


A network of SMG-8, SMG-9 and SMG-1 C-terminal insertion domain regulates UPF1 substrate recruitment and phosphorylation.

Deniaud A, Karuppasamy M, Bock T, Masiulis S, Huard K, Garzoni F, Kerschgens K, Hentze MW, Kulozik AE, Beck M, Neu-Yilik G, Schaffitzel C.

Nucleic Acids Res. 2015 Sep 3;43(15):7600-11. doi: 10.1093/nar/gkv668. Epub 2015 Jun 30.


Molecular Basis of the Rapamycin Insensitivity of Target Of Rapamycin Complex 2.

Gaubitz C, Oliveira TM, Prouteau M, Leitner A, Karuppasamy M, Konstantinidou G, Rispal D, Eltschinger S, Robinson GC, Thore S, Aebersold R, Schaffitzel C, Loewith R.

Mol Cell. 2015 Jun 18;58(6):977-88. doi: 10.1016/j.molcel.2015.04.031. Epub 2015 May 28.


Advances and challenges of membrane-protein complex production.

Zorman S, Botte M, Jiang Q, Collinson I, Schaffitzel C.

Curr Opin Struct Biol. 2015 Jun;32:123-30. doi: 10.1016/ Epub 2015 Apr 15. Review.


ACEMBLing a multiprotein transmembrane complex: the functional SecYEG-SecDF-YajC-YidC Holotranslocon protein secretase/insertase.

Komar J, Botte M, Collinson I, Schaffitzel C, Berger I.

Methods Enzymol. 2015;556:23-49. doi: 10.1016/bs.mie.2014.12.027. Epub 2015 Mar 20.


Ribosome-SRP-FtsY cotranslational targeting complex in the closed state.

von Loeffelholz O, Jiang Q, Ariosa A, Karuppasamy M, Huard K, Berger I, Shan SO, Schaffitzel C.

Proc Natl Acad Sci U S A. 2015 Mar 31;112(13):3943-8. doi: 10.1073/pnas.1424453112. Epub 2015 Mar 16.


Cytoplasmic TAF2-TAF8-TAF10 complex provides evidence for nuclear holo-TFIID assembly from preformed submodules.

Trowitzsch S, Viola C, Scheer E, Conic S, Chavant V, Fournier M, Papai G, Ebong IO, Schaffitzel C, Zou J, Haffke M, Rappsilber J, Robinson CV, Schultz P, Tora L, Berger I.

Nat Commun. 2015 Jan 14;6:6011. doi: 10.1038/ncomms7011.


Continuous fluorescence-based measurement of redox-driven sodium ion translocation.

Muras V, Claussen B, Karuppasamy M, Schaffitzel C, Steuber J.

Anal Biochem. 2014 Aug 15;459:53-5. doi: 10.1016/j.ab.2014.05.012. Epub 2014 May 23.


Membrane protein insertion and proton-motive-force-dependent secretion through the bacterial holo-translocon SecYEG-SecDF-YajC-YidC.

Schulze RJ, Komar J, Botte M, Allen WJ, Whitehouse S, Gold VA, Lycklama A Nijeholt JA, Huard K, Berger I, Schaffitzel C, Collinson I.

Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):4844-9. doi: 10.1073/pnas.1315901111. Epub 2014 Feb 18.


Structural and functional analysis of the three MIF4G domains of nonsense-mediated decay factor UPF2.

Clerici M, Deniaud A, Boehm V, Gehring NH, Schaffitzel C, Cusack S.

Nucleic Acids Res. 2014 Feb;42(4):2673-86. doi: 10.1093/nar/gkt1197. Epub 2013 Nov 23.


Structural basis of signal sequence surveillance and selection by the SRP-FtsY complex.

von Loeffelholz O, Knoops K, Ariosa A, Zhang X, Karuppasamy M, Huard K, Schoehn G, Berger I, Shan SO, Schaffitzel C.

Nat Struct Mol Biol. 2013 May;20(5):604-10. doi: 10.1038/nsmb.2546. Epub 2013 Apr 7.


Directly from the source: endogenous preparations of molecular machines.

Mesa P, Deniaud A, Montoya G, Schaffitzel C.

Curr Opin Struct Biol. 2013 Jun;23(3):319-25. doi: 10.1016/ Epub 2013 Feb 8. Review.


The architecture of human general transcription factor TFIID core complex.

Bieniossek C, Papai G, Schaffitzel C, Garzoni F, Chaillet M, Scheer E, Papadopoulos P, Tora L, Schultz P, Berger I.

Nature. 2013 Jan 31;493(7434):699-702. doi: 10.1038/nature11791. Epub 2013 Jan 6.


Low-resolution structure determination of Na(+)-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae by ab initio phasing and electron microscopy.

Lunin VY, Lunina NL, Casutt MS, Knoops K, Schaffitzel C, Steuber J, Fritz G, Baumstark MW.

Acta Crystallogr D Biol Crystallogr. 2012 Jun;68(Pt 6):724-31. doi: 10.1107/S0907444912012012. Epub 2012 May 17.


Cryo-electron microscopy of ribosomal complexes in cotranslational folding, targeting, and translocation.

Knoops K, Schoehn G, Schaffitzel C.

Wiley Interdiscip Rev RNA. 2012 May-Jun;3(3):429-41. doi: 10.1002/wrna.119. Epub 2011 Nov 17. Review.


Robots, pipelines, polyproteins: enabling multiprotein expression in prokaryotic and eukaryotic cells.

Vijayachandran LS, Viola C, Garzoni F, Trowitzsch S, Bieniossek C, Chaillet M, Schaffitzel C, Busso D, Romier C, Poterszman A, Richmond TJ, Berger I.

J Struct Biol. 2011 Aug;175(2):198-208. doi: 10.1016/j.jsb.2011.03.007. Epub 2011 Mar 17. Review.


Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor.

Estrozi LF, Boehringer D, Shan SO, Ban N, Schaffitzel C.

Nat Struct Mol Biol. 2011 Jan;18(1):88-90. doi: 10.1038/nsmb.1952. Epub 2010 Dec 12.


Probing telomeric G-quadruplex DNA structures in cells with in vitro generated single-chain antibody fragments.

Schaffitzel C, Postberg J, Paeschke K, Lipps HJ.

Methods Mol Biol. 2010;608:159-81. doi: 10.1007/978-1-59745-363-9_11.


YidC and Oxa1 form dimeric insertion pores on the translating ribosome.

Kohler R, Boehringer D, Greber B, Bingel-Erlenmeyer R, Collinson I, Schaffitzel C, Ban N.

Mol Cell. 2009 May 15;34(3):344-53. doi: 10.1016/j.molcel.2009.04.019.


Automated unrestricted multigene recombineering for multiprotein complex production.

Bieniossek C, Nie Y, Frey D, Olieric N, Schaffitzel C, Collinson I, Romier C, Berger P, Richmond TJ, Steinmetz MO, Berger I.

Nat Methods. 2009 Jun;6(6):447-50. doi: 10.1038/nmeth.1326. Epub 2009 May 3.


Multiple conformational switches in a GTPase complex control co-translational protein targeting.

Zhang X, Schaffitzel C, Ban N, Shan SO.

Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1754-9. doi: 10.1073/pnas.0808573106. Epub 2009 Jan 27.


Recycling of aborted ribosomal 50S subunit-nascent chain-tRNA complexes by the heat shock protein Hsp15.

Jiang L, Schaffitzel C, Bingel-Erlenmeyer R, Ban N, Korber P, Koning RI, de Geus DC, Plaisier JR, Abrahams JP.

J Mol Biol. 2009 Mar 13;386(5):1357-67. doi: 10.1016/j.jmb.2008.10.079. Epub 2008 Nov 5.


Molecular mechanism and structure of Trigger Factor bound to the translating ribosome.

Merz F, Boehringer D, Schaffitzel C, Preissler S, Hoffmann A, Maier T, Rutkowska A, Lozza J, Ban N, Bukau B, Deuerling E.

EMBO J. 2008 Jun 4;27(11):1622-32. doi: 10.1038/emboj.2008.89. Epub 2008 May 22.


A peptide deformylase-ribosome complex reveals mechanism of nascent chain processing.

Bingel-Erlenmeyer R, Kohler R, Kramer G, Sandikci A, Antolić S, Maier T, Schaffitzel C, Wiedmann B, Bukau B, Ban N.

Nature. 2008 Mar 6;452(7183):108-11. doi: 10.1038/nature06683. Epub 2008 Feb 20.


Dynamics of trigger factor interaction with translating ribosomes.

Rutkowska A, Mayer MP, Hoffmann A, Merz F, Zachmann-Brand B, Schaffitzel C, Ban N, Deuerling E, Bukau B.

J Biol Chem. 2008 Feb 15;283(7):4124-32. Epub 2007 Nov 28.


Transport of Na(+) and K (+) by an antiporter-related subunit from the Escherichia coli NADH dehydrogenase I produced in Saccharomyces cerevisiae.

Gemperli AC, Schaffitzel C, Jakob C, Steuber J.

Arch Microbiol. 2007 Nov;188(5):509-21. Epub 2007 Jun 22.


Multiprotein expression strategy for structural biology of eukaryotic complexes.

Fitzgerald DJ, Schaffitzel C, Berger P, Wellinger R, Bieniossek C, Richmond TJ, Berger I.

Structure. 2007 Mar;15(3):275-9. Erratum in: Structure. 2007 Sep;15(9):1156.


Generation of ribosome nascent chain complexes for structural and functional studies.

Schaffitzel C, Ban N.

J Struct Biol. 2007 Jun;158(3):463-71. Epub 2007 Jan 23.


Protein complex expression by using multigene baculoviral vectors.

Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, Berger I.

Nat Methods. 2006 Dec;3(12):1021-32.


Structure of the E. coli signal recognition particle bound to a translating ribosome.

Schaffitzel C, Oswald M, Berger I, Ishikawa T, Abrahams JP, Koerten HK, Koning RI, Ban N.

Nature. 2006 Nov 23;444(7118):503-6. Epub 2006 Oct 29. Erratum in: Nature. 2007 Aug 30;448(7157):1076.


Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements.

Mitra K, Schaffitzel C, Fabiola F, Chapman MS, Ban N, Frank J.

Mol Cell. 2006 May 19;22(4):533-43.


Multi-level regulation of myotubularin-related protein-2 phosphatase activity by myotubularin-related protein-13/set-binding factor-2.

Berger P, Berger I, Schaffitzel C, Tersar K, Volkmer B, Suter U.

Hum Mol Genet. 2006 Feb 15;15(4):569-79. Epub 2006 Jan 6.


Structure of the E. coli protein-conducting channel bound to a translating ribosome.

Mitra K, Schaffitzel C, Shaikh T, Tama F, Jenni S, Brooks CL 3rd, Ban N, Frank J.

Nature. 2005 Nov 17;438(7066):318-24.


Membrane association of myotubularin-related protein 2 is mediated by a pleckstrin homology-GRAM domain and a coiled-coil dimerization module.

Berger P, Schaffitzel C, Berger I, Ban N, Suter U.

Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12177-82. Epub 2003 Oct 6.


Direct interaction of Ca2+/calmodulin inhibits histone deacetylase 5 repressor core binding to myocyte enhancer factor 2.

Berger I, Bieniossek C, Schaffitzel C, Hassler M, Santelli E, Richmond TJ.

J Biol Chem. 2003 May 16;278(20):17625-35. Epub 2003 Mar 6.

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