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Items: 1 to 50 of 90

1.

What we talk about when we talk about nanoclusters.

Baumgart F, Arnold AM, Rossboth BK, Brameshuber M, Schütz GJ.

Methods Appl Fluoresc. 2018 Nov 9;7(1):013001. doi: 10.1088/2050-6120/aaed0f.

PMID:
30412469
2.

Single-molecule optical absorption imaging by nanomechanical photothermal sensing.

Chien MH, Brameshuber M, Rossboth BK, Schütz GJ, Schmid S.

Proc Natl Acad Sci U S A. 2018 Oct 30;115(44):11150-11155. doi: 10.1073/pnas.1804174115. Epub 2018 Sep 25.

3.

Direct observation of cargo transfer from HDL particles to the plasma membrane.

Plochberger B, Axmann M, Röhrl C, Weghuber J, Brameshuber M, Rossboth BK, Mayr S, Ros R, Bittman R, Stangl H, Schütz GJ.

Atherosclerosis. 2018 Oct;277:53-59. doi: 10.1016/j.atherosclerosis.2018.08.032. Epub 2018 Aug 27.

4.

Tunable DNA Hybridization Enables Spatially and Temporally Controlled Surface-Anchoring of Biomolecular Cargo.

Hager R, Arnold A, Sevcsik E, Schütz GJ, Howorka S.

Langmuir. 2018 Aug 30. doi: 10.1021/acs.langmuir.8b01942. [Epub ahead of print]

PMID:
30160973
5.

TCRs are randomly distributed on the plasma membrane of resting antigen-experienced T cells.

Rossboth B, Arnold AM, Ta H, Platzer R, Kellner F, Huppa JB, Brameshuber M, Baumgart F, Schütz GJ.

Nat Immunol. 2018 Aug;19(8):821-827. doi: 10.1038/s41590-018-0162-7. Epub 2018 Jul 16.

PMID:
30013143
6.

Determination of the Membrane Environment of CD59 in Living Cells.

Fülöp G, Brameshuber M, Arnold AM, Schütz GJ, Sevcsik E.

Biomolecules. 2018 May 17;8(2). pii: E28. doi: 10.3390/biom8020028.

7.

Monomeric TCRs drive T cell antigen recognition.

Brameshuber M, Kellner F, Rossboth BK, Ta H, Alge K, Sevcsik E, Göhring J, Axmann M, Baumgart F, Gascoigne NRJ, Davis SJ, Stockinger H, Schütz GJ, Huppa JB.

Nat Immunol. 2018 May;19(5):487-496. doi: 10.1038/s41590-018-0092-4. Epub 2018 Apr 16.

PMID:
29662172
8.

Kinetic analysis of single molecule FRET transitions without trajectories.

Schrangl L, Göhring J, Schütz GJ.

J Chem Phys. 2018 Mar 28;148(12):123328. doi: 10.1063/1.5006038.

PMID:
29604857
9.

HDL particles incorporate into lipid bilayers - a combined AFM and single molecule fluorescence microscopy study.

Plochberger B, Röhrl C, Preiner J, Rankl C, Brameshuber M, Madl J, Bittman R, Ros R, Sezgin E, Eggeling C, Hinterdorfer P, Stangl H, Schütz GJ.

Sci Rep. 2017 Nov 21;7(1):15886. doi: 10.1038/s41598-017-15949-7.

10.

Mild heat induces a distinct "eustress" response in Chinese Hamster Ovary cells but does not induce heat shock protein synthesis.

Peksel B, Gombos I, Péter M, Vigh L Jr, Tiszlavicz Á, Brameshuber M, Balogh G, Schütz GJ, Horváth I, Vigh L, Török Z.

Sci Rep. 2017 Nov 15;7(1):15643. doi: 10.1038/s41598-017-15821-8.

11.

Förster Resonance Energy Transfer to Study TCR-pMHC Interactions in the Immunological Synapse.

Schütz GJ, Huppa JB.

Methods Mol Biol. 2017;1584:207-229. doi: 10.1007/978-1-4939-6881-7_14.

PMID:
28255705
12.

Protein Micropatterning Assay: Quantitative Analysis of Protein-Protein Interactions.

Schütz GJ, Weghuber J, Lanzerstorfer P, Sevcsik E.

Methods Mol Biol. 2017;1550:261-270. doi: 10.1007/978-1-4939-6747-6_18.

PMID:
28188535
13.

Direct PIP2 binding mediates stable oligomer formation of the serotonin transporter.

Anderluh A, Hofmaier T, Klotzsch E, Kudlacek O, Stockner T, Sitte HH, Schütz GJ.

Nat Commun. 2017 Jan 19;8:14089. doi: 10.1038/ncomms14089.

14.

There Is No Simple Model of the Plasma Membrane Organization.

Bernardino de la Serna J, Schütz GJ, Eggeling C, Cebecauer M.

Front Cell Dev Biol. 2016 Sep 29;4:106. eCollection 2016. Review.

15.

Varying label density allows artifact-free analysis of membrane-protein nanoclusters.

Baumgart F, Arnold AM, Leskovar K, Staszek K, Fölser M, Weghuber J, Stockinger H, Schütz GJ.

Nat Methods. 2016 Aug;13(8):661-4. doi: 10.1038/nmeth.3897. Epub 2016 Jun 13.

PMID:
27295310
16.

Stimulated Emission Depletion Lithography with Mercapto-Functional Polymers.

Buchegger B, Kreutzer J, Plochberger B, Wollhofen R, Sivun D, Jacak J, Schütz GJ, Schubert U, Klar TA.

ACS Nano. 2016 Feb 23;10(2):1954-9. doi: 10.1021/acsnano.5b05863. Epub 2016 Feb 10.

17.

Oxidized Phospholipids Inhibit the Formation of Cholesterol-Dependent Plasma Membrane Nanoplatforms.

Brameshuber M, Sevcsik E, Rossboth BK, Manner C, Deigner HP, Peksel B, Péter M, Török Z, Hermetter A, Schütz GJ.

Biophys J. 2016 Jan 5;110(1):205-13. doi: 10.1016/j.bpj.2015.11.018.

18.

With or without rafts? Alternative views on cell membranes.

Sevcsik E, Schütz GJ.

Bioessays. 2016 Feb;38(2):129-39. doi: 10.1002/bies.201500150. Epub 2015 Dec 15. Review.

PMID:
26666984
19.

A microfluidic platform for transcription- and amplification-free detection of zepto-mole amounts of nucleic acid molecules.

Mayr R, Haider M, Thünauer R, Haselgrübler T, Schütz GJ, Sonnleitner A, Hesse J.

Biosens Bioelectron. 2016 Apr 15;78:1-6. doi: 10.1016/j.bios.2015.11.013. Epub 2015 Nov 10.

PMID:
26580983
20.

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers.

Axmann M, Schütz GJ, Huppa JB.

J Vis Exp. 2015 Oct 31;(105):e53158. doi: 10.3791/53158.

21.

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay.

Axmann M, Schütz GJ, Huppa JB.

J Vis Exp. 2015 Oct 30;(105):e53157. doi: 10.3791/53157.

22.

GPI-anchored proteins do not reside in ordered domains in the live cell plasma membrane.

Sevcsik E, Brameshuber M, Fölser M, Weghuber J, Honigmann A, Schütz GJ.

Nat Commun. 2015 Apr 21;6:6969. doi: 10.1038/ncomms7969.

23.

Cooperativity between individual transporter protomers: new data fuelling old complexes.

Sitte HH, Schütz GJ, Freissmuth M.

J Neurochem. 2015 Apr;133(2):163-6. doi: 10.1111/jnc.13086. Epub 2015 Mar 15.

24.

Superresolution microscopy reveals spatial separation of UCP4 and F0F1-ATP synthase in neuronal mitochondria.

Klotzsch E, Smorodchenko A, Löfler L, Moldzio R, Parkinson E, Schütz GJ, Pohl EE.

Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):130-5. doi: 10.1073/pnas.1415261112. Epub 2014 Dec 22.

25.

Detecting protein association at the T cell plasma membrane.

Baumgart F, Schütz GJ.

Biochim Biophys Acta. 2015 Apr;1853(4):791-801. doi: 10.1016/j.bbamcr.2014.09.026. Epub 2014 Oct 6. Review.

26.

Tracking single serotonin transporter molecules at the endoplasmic reticulum and plasma membrane.

Anderluh A, Klotzsch E, Ries J, Reismann AW, Weber S, Fölser M, Koban F, Freissmuth M, Sitte HH, Schütz GJ.

Biophys J. 2014 May 6;106(9):L33-5. doi: 10.1016/j.bpj.2014.03.019.

27.

Lck mediates signal transmission from CD59 to the TCR/CD3 pathway in Jurkat T cells.

Lipp AM, Juhasz K, Paar C, Ogris C, Eckerstorfer P, Thuenauer R, Hesse J, Nimmervoll B, Stockinger H, Schütz GJ, Bodenhofer U, Balogi Z, Sonnleitner A.

PLoS One. 2014 Jan 15;9(1):e85934. doi: 10.1371/journal.pone.0085934. eCollection 2014.

28.

Single molecule analysis reveals coexistence of stable serotonin transporter monomers and oligomers in the live cell plasma membrane.

Anderluh A, Klotzsch E, Reismann AW, Brameshuber M, Kudlacek O, Newman AH, Sitte HH, Schütz GJ.

J Biol Chem. 2014 Feb 14;289(7):4387-94. doi: 10.1074/jbc.M113.531632. Epub 2014 Jan 6.

29.

Plasma membranes as heat stress sensors: from lipid-controlled molecular switches to therapeutic applications.

Török Z, Crul T, Maresca B, Schütz GJ, Viana F, Dindia L, Piotto S, Brameshuber M, Balogh G, Péter M, Porta A, Trapani A, Gombos I, Glatz A, Gungor B, Peksel B, Vigh L Jr, Csoboz B, Horváth I, Vijayan MM, Hooper PL, Harwood JL, Vigh L.

Biochim Biophys Acta. 2014 Jun;1838(6):1594-618. doi: 10.1016/j.bbamem.2013.12.015. Epub 2013 Dec 27. Review.

30.

Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells.

Fuertbauer E, Zaujec J, Uhrin P, Raab I, Weber M, Schachner H, Bauer M, Schütz GJ, Binder BR, Sixt M, Kerjaschki D, Stockinger H.

Immunol Lett. 2013 Jul-Aug;154(1-2):31-41. doi: 10.1016/j.imlet.2013.07.007. Epub 2013 Jul 30.

PMID:
23912054
31.

Improved ligand discrimination by force-induced unbinding of the T cell receptor from peptide-MHC.

Klotzsch E, Schütz GJ.

Biophys J. 2013 Apr 16;104(8):1670-5. doi: 10.1016/j.bpj.2013.03.023.

32.

Expression analysis of multiple myeloma CD138 negative progenitor cells using single molecule microarray readout.

Jacak J, Schnidar H, Muresan L, Regl G, Frischauf A, Aberger F, Schütz GJ, Hesse J.

J Biotechnol. 2013 Apr 15;164(4):525-30. doi: 10.1016/j.jbiotec.2013.01.027. Epub 2013 Feb 14.

33.

A critical survey of methods to detect plasma membrane rafts.

Klotzsch E, Schütz GJ.

Philos Trans R Soc Lond B Biol Sci. 2012 Dec 24;368(1611):20120033. doi: 10.1098/rstb.2012.0033. Print 2013 Feb 5. Review.

34.

Determination of binding curves via protein micropatterning in vitro and in living cells.

Sunzenauer S, Zojer V, Brameshuber M, Tröls A, Weghuber J, Stockinger H, Schütz GJ.

Cytometry A. 2013 Sep;83(9):847-54. doi: 10.1002/cyto.a.22225. Epub 2012 Nov 2.

35.

Determination of interaction kinetics between the T cell receptor and peptide-loaded MHC class II via single-molecule diffusion measurements.

Axmann M, Huppa JB, Davis MM, Schütz GJ.

Biophys J. 2012 Jul 18;103(2):L17-9. doi: 10.1016/j.bpj.2012.06.019. Epub 2012 Jul 17.

36.

Detection and quantification of biomolecular association in living cells using single-molecule microscopy.

Brameshuber M, Schütz GJ.

Methods Enzymol. 2012;505:159-86. doi: 10.1016/B978-0-12-388448-0.00017-6.

PMID:
22289453
37.

VEGF-induced endothelial cell migration requires urokinase receptor (uPAR)-dependent integrin redistribution.

Alexander RA, Prager GW, Mihaly-Bison J, Uhrin P, Sunzenauer S, Binder BR, Schütz GJ, Freissmuth M, Breuss JM.

Cardiovasc Res. 2012 Apr 1;94(1):125-35. doi: 10.1093/cvr/cvs017. Epub 2012 Jan 26.

38.

Membrane-lipid therapy in operation: the HSP co-inducer BGP-15 activates stress signal transduction pathways by remodeling plasma membrane rafts.

Gombos I, Crul T, Piotto S, Güngör B, Török Z, Balogh G, Péter M, Slotte JP, Campana F, Pilbat AM, Hunya A, Tóth N, Literati-Nagy Z, Vígh L Jr, Glatz A, Brameshuber M, Schütz GJ, Hevener A, Febbraio MA, Horváth I, Vígh L.

PLoS One. 2011;6(12):e28818. doi: 10.1371/journal.pone.0028818. Epub 2011 Dec 12.

39.

What can we learn from single molecule trajectories?

Ruprecht V, Axmann M, Wieser S, Schutz GJ.

Curr Protein Pept Sci. 2011 Dec;12(8):714-24. Review.

PMID:
22044145
40.

Cationic amphipathic peptides accumulate sialylated proteins and lipids in the plasma membrane of eukaryotic host cells.

Weghuber J, Aichinger MC, Brameshuber M, Wieser S, Ruprecht V, Plochberger B, Madl J, Horner A, Reipert S, Lohner K, Henics T, Schütz GJ.

Biochim Biophys Acta. 2011 Oct;1808(10):2581-90. doi: 10.1016/j.bbamem.2011.06.007. Epub 2011 Jun 28.

41.

Spot variation fluorescence correlation spectroscopy allows for superresolution chronoscopy of confinement times in membranes.

Ruprecht V, Wieser S, Marguet D, Schütz GJ.

Biophys J. 2011 Jun 8;100(11):2839-45. doi: 10.1016/j.bpj.2011.04.035.

42.

Single-molecule detection on a protein-array assay platform for the exposure of a tuberculosis antigen.

Schmidt R, Jacak J, Schirwitz C, Stadler V, Michel G, Marmé N, Schütz GJ, Hoheisel JD, Knemeyer JP.

J Proteome Res. 2011 Mar 4;10(3):1316-22. doi: 10.1021/pr101070j. Epub 2011 Feb 16.

PMID:
21247063
43.

Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane.

Brameshuber M, Weghuber J, Ruprecht V, Gombos I, Horváth I, Vigh L, Eckerstorfer P, Kiss E, Stockinger H, Schütz GJ.

J Biol Chem. 2010 Dec 31;285(53):41765-71. doi: 10.1074/jbc.M110.182121. Epub 2010 Oct 21.

44.

Resting state Orai1 diffuses as homotetramer in the plasma membrane of live mammalian cells.

Madl J, Weghuber J, Fritsch R, Derler I, Fahrner M, Frischauf I, Lackner B, Romanin C, Schütz GJ.

J Biol Chem. 2010 Dec 24;285(52):41135-42. doi: 10.1074/jbc.M110.177881. Epub 2010 Oct 20.

45.

Cholesterol slows down the lateral mobility of an oxidized phospholipid in a supported lipid bilayer.

Plochberger B, Stockner T, Chiantia S, Brameshuber M, Weghuber J, Hermetter A, Schwille P, Schütz GJ.

Langmuir. 2010 Nov 16;26(22):17322-9. doi: 10.1021/la1026202. Epub 2010 Oct 13.

46.

Antimicrobial and immunostimulatory peptide, KLK, induces an increase in cytosolic Ca²(+) concentration by mobilizing Ca²(+) from intracellular stores.

Weghuber J, Lipp AM, Stadlbauer J, Aichinger MC, Ruprecht V, Sonnleitner A, Schütz GJ, Henics T.

Cell Biol Int. 2010 Nov;34(11):1109-12. doi: 10.1042/CBI20100408.

PMID:
20695847
47.

Detection of protein-protein interactions in the live cell plasma membrane by quantifying prey redistribution upon bait micropatterning.

Weghuber J, Brameshuber M, Sunzenauer S, Lehner M, Paar C, Haselgrübler T, Schwarzenbacher M, Kaltenbrunner M, Hesch C, Paster W, Heise B, Sonnleitner A, Stockinger H, Schütz GJ.

Methods Enzymol. 2010;472:133-51. doi: 10.1016/S0076-6879(10)72012-7.

PMID:
20580963
48.

Temporal resolution of protein-protein interactions in the live-cell plasma membrane.

Weghuber J, Sunzenauer S, Plochberger B, Brameshuber M, Haselgrübler T, Schütz GJ.

Anal Bioanal Chem. 2010 Aug;397(8):3339-47. doi: 10.1007/s00216-010-3854-x. Epub 2010 Jun 25.

49.

Identification of immobile single molecules using polarization-modulated asynchronous time delay and integration-mode scanning.

Jacak J, Hesch C, Hesse J, Schütz GJ.

Anal Chem. 2010 May 15;82(10):4288-92. doi: 10.1021/ac100302s.

50.

in-vivo detection of protein-protein interactions on micro-patterned surfaces.

Weghuber J, Sunzenauer S, Brameshuber M, Plochberger B, Hesch C, Schutz GJ.

J Vis Exp. 2010 Mar 19;(37). pii: 1969. doi: 10.3791/1969.

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