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Items: 1 to 50 of 58

1.

Identification of 15 New Bypassable Essential Genes of Fission Yeast.

Takeda A, Saitoh S, Ohkura H, Sawin KE, Goshima G.

Cell Struct Funct. 2019 Sep 27;44(2):113-119. doi: 10.1247/csf.19025. Epub 2019 Aug 31. Erratum in: Cell Struct Funct. 2019;44(2):171.

2.

Reconstitution of Microtubule Nucleation In Vitro Reveals Novel Roles for Mzt1.

Leong SL, Lynch EM, Zou J, Tay YD, Borek WE, Tuijtel MW, Rappsilber J, Sawin KE.

Curr Biol. 2019 Jul 8;29(13):2199-2207.e10. doi: 10.1016/j.cub.2019.05.058.

3.

Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD, Leda M, Spanos C, Rappsilber J, Goryachev AB, Sawin KE.

Cell Rep. 2019 Feb 5;26(6):1654-1667.e7. doi: 10.1016/j.celrep.2019.01.027.

4.

Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.

Tay YD, Leda M, Goryachev AB, Sawin KE.

J Cell Sci. 2018 Jul 19;131(14). pii: jcs216580. doi: 10.1242/jcs.216580.

5.

Exportin Crm1 is repurposed as a docking protein to generate microtubule organizing centers at the nuclear pore.

Bao XX, Spanos C, Kojidani T, Lynch EM, Rappsilber J, Hiraoka Y, Haraguchi T, Sawin KE.

Elife. 2018 May 29;7. pii: e33465. doi: 10.7554/eLife.33465.

6.

Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Technology in Fission Yeast.

Maček B, Carpy A, Koch A, Bicho CC, Borek WE, Hauf S, Sawin KE.

Cold Spring Harb Protoc. 2017 Jun 1;2017(6):pdb.top079814. doi: 10.1101/pdb.top079814.

PMID:
28572211
7.

Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-Based Quantitative Proteomics and Phosphoproteomics in Fission Yeast.

Carpy A, Koch A, Bicho CC, Borek WE, Hauf S, Sawin KE, Maček B.

Cold Spring Harb Protoc. 2017 Jun 1;2017(6):pdb.prot091686. doi: 10.1101/pdb.prot091686.

PMID:
28572185
8.

Construction, Growth, and Harvesting of Fission Yeast Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Strains.

Koch A, Bicho CC, Borek WE, Carpy A, Maček B, Hauf S, Sawin KE.

Cold Spring Harb Protoc. 2017 Jun 1;2017(6):pdb.prot091678. doi: 10.1101/pdb.prot091678.

PMID:
28572184
9.

Remodeling of the Fission Yeast Cdc42 Cell-Polarity Module via the Sty1 p38 Stress-Activated Protein Kinase Pathway.

Mutavchiev DR, Leda M, Sawin KE.

Curr Biol. 2016 Nov 7;26(21):2921-2928. doi: 10.1016/j.cub.2016.08.048. Epub 2016 Oct 13.

10.

Mto2 multisite phosphorylation inactivates non-spindle microtubule nucleation complexes during mitosis.

Borek WE, Groocock LM, Samejima I, Zou J, de Lima Alves F, Rappsilber J, Sawin KE.

Nat Commun. 2015 Aug 5;6:7929. doi: 10.1038/ncomms8929.

11.

Deletion of Genes Encoding Arginase Improves Use of "Heavy" Isotope-Labeled Arginine for Mass Spectrometry in Fission Yeast.

Borek WE, Zou J, Rappsilber J, Sawin KE.

PLoS One. 2015 Jun 15;10(6):e0129548. doi: 10.1371/journal.pone.0129548. eCollection 2015.

12.

Pom1 regulates the assembly of Cdr2-Mid1 cortical nodes for robust spatial control of cytokinesis.

Rincon SA, Bhatia P, Bicho C, Guzman-Vendrell M, Fraisier V, Borek WE, Alves Fde L, Dingli F, Loew D, Rappsilber J, Sawin KE, Martin SG, Paoletti A.

J Cell Biol. 2014 Jul 7;206(1):61-77. doi: 10.1083/jcb.201311097. Epub 2014 Jun 30.

13.

Activation of the γ-tubulin complex by the Mto1/2 complex.

Lynch EM, Groocock LM, Borek WE, Sawin KE.

Curr Biol. 2014 Apr 14;24(8):896-903. doi: 10.1016/j.cub.2014.03.006. Epub 2014 Apr 3.

14.

Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.

Snaith HA, Thompson J, Yates JR 3rd, Sawin KE.

J Cell Sci. 2011 Jul 1;124(Pt 13):2187-99. doi: 10.1242/jcs.084038. Epub 2011 Jun 7.

15.

Microtubule stabilization in vivo by nucleation-incompetent γ-tubulin complex.

Anders A, Sawin KE.

J Cell Sci. 2011 Apr 15;124(Pt 8):1207-13. doi: 10.1242/jcs.083741.

16.

Fission yeast Mto1 regulates diversity of cytoplasmic microtubule organizing centers.

Samejima I, Miller VJ, Rincon SA, Sawin KE.

Curr Biol. 2010 Nov 9;20(21):1959-65. doi: 10.1016/j.cub.2010.10.006. Epub 2010 Oct 21.

17.

A catalytic role for Mod5 in the formation of the Tea1 cell polarity landmark.

Bicho CC, Kelly DA, Snaith HA, Goryachev AB, Sawin KE.

Curr Biol. 2010 Oct 12;20(19):1752-7. doi: 10.1016/j.cub.2010.08.035. Epub 2010 Sep 16.

18.

New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation.

Snaith HA, Anders A, Samejima I, Sawin KE.

Methods Cell Biol. 2010;97:147-72. doi: 10.1016/S0091-679X(10)97009-X. Review.

PMID:
20719270
19.

A genetic engineering solution to the "arginine conversion problem" in stable isotope labeling by amino acids in cell culture (SILAC).

Bicho CC, de Lima Alves F, Chen ZA, Rappsilber J, Sawin KE.

Mol Cell Proteomics. 2010 Jul;9(7):1567-77. doi: 10.1074/mcp.M110.000208. Epub 2010 May 10.

20.

Inexpensive synthetic-based matrix for both conventional and rapid purification of protein A- and tandem affinity purification-tagged proteins.

Sawin KE, Bicho CC, Snaith HA.

Anal Biochem. 2010 Feb 15;397(2):241-3. doi: 10.1016/j.ab.2009.09.045. Epub 2009 Sep 30.

21.

Cell cycle: Cell division brought down to size.

Sawin KE.

Nature. 2009 Jun 11;459(7248):782-3. doi: 10.1038/459782a. No abstract available.

PMID:
19516326
23.

Two distinct regions of Mto1 are required for normal microtubule nucleation and efficient association with the gamma-tubulin complex in vivo.

Samejima I, Miller VJ, Groocock LM, Sawin KE.

J Cell Sci. 2008 Dec 1;121(Pt 23):3971-80. doi: 10.1242/jcs.038414. Epub 2008 Nov 11.

24.

Comment on "A centrosome-independent role for gamma-TuRC proteins in the spindle assembly checkpoint".

Taylor SS, Hardwick KG, Sawin KE, Biggins S, Piatti S, Khodjakov A, Rieder CL, Salmon ED, Musacchio A.

Science. 2007 May 18;316(5827):982; author reply 982.

25.

Cell division: mid-level management.

Sawin KE.

Curr Biol. 2007 Feb 6;17(3):R93-5.

26.

Cytoplasmic microtubule organization in fission yeast.

Sawin KE, Tran PT.

Yeast. 2006 Oct 15;23(13):1001-14. Review.

27.

Noncore components of the fission yeast gamma-tubulin complex.

Anders A, Lourenço PC, Sawin KE.

Mol Biol Cell. 2006 Dec;17(12):5075-93. Epub 2006 Oct 4.

28.

Multistep and multimode cortical anchoring of tea1p at cell tips in fission yeast.

Snaith HA, Samejima I, Sawin KE.

EMBO J. 2005 Nov 2;24(21):3690-9. Epub 2005 Oct 13.

29.

Meiosis: organizing microtubule organizers.

Sawin KE.

Curr Biol. 2005 Aug 23;15(16):R633-5. Review.

30.

Tea for three: control of fission yeast polarity.

Snaith HA, Sawin KE.

Nat Cell Biol. 2005 May;7(5):450-1. No abstract available.

PMID:
15867927
31.

Fission yeast mto2p regulates microtubule nucleation by the centrosomin-related protein mto1p.

Samejima I, Lourenço PC, Snaith HA, Sawin KE.

Mol Biol Cell. 2005 Jun;16(6):3040-51. Epub 2005 Jan 19.

32.

Microtubule dynamics: faint speckle, hidden dragon.

Sawin KE.

Curr Biol. 2004 Sep 7;14(17):R702-4. Review.

34.

The spindle gets bigger.

Sawin KE.

Cell. 2004 Apr 30;117(3):281-3.

35.

Role of microtubules and tea1p in establishment and maintenance of fission yeast cell polarity.

Sawin KE, Snaith HA.

J Cell Sci. 2004 Feb 15;117(Pt 5):689-700. Epub 2004 Jan 20.

36.

Fission yeast mod5p regulates polarized growth through anchoring of tea1p at cell tips.

Snaith HA, Sawin KE.

Nature. 2003 Jun 5;423(6940):647-51.

PMID:
12789340
37.

Cell polarity: following formin function.

Sawin KE.

Curr Biol. 2002 Jan 8;12(1):R6-8.

38.

Microtubule dynamics: the view from the tip.

Sawin KE.

Curr Biol. 2000 Nov 30;10(23):R860-2.

39.

Cytokinesis: Sid signals septation.

Sawin KE.

Curr Biol. 2000 Jul 27-Aug 10;10(15):R547-50. Review. No abstract available.

40.

Mis-specification of cortical identity in a fission yeast PAK mutant.

Sawin KE, Hajibagheri MA, Nurse P.

Curr Biol. 1999 Nov 18;9(22):1335-8.

41.

Some thoughts about microtubules and cell polarity in fission yeast.

Sawin KE.

Fungal Genet Biol. 1999 Jul-Aug;27(2-3):224-30. Review. No abstract available.

PMID:
10441448
42.

GFP fusion proteins as probes for cytology in fission yeast.

Sawin KE.

Methods Cell Biol. 1999;58:123-38. Review. No abstract available.

PMID:
9891378
43.

Regulation of cell polarity by microtubules in fission yeast.

Sawin KE, Nurse P.

J Cell Biol. 1998 Jul 27;142(2):457-71.

44.

Caged fluorescent probes.

Mitchison TJ, Sawin KE, Theriot JA, Gee K, Mallavarapu A.

Methods Enzymol. 1998;291:63-78. No abstract available.

PMID:
9661145
45.

Photoactivation of green fluorescent protein.

Sawin KE, Nurse P.

Curr Biol. 1997 Oct 1;7(10):R606-7. No abstract available.

46.
47.

Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle.

Sawin KE, Mitchison TJ.

Proc Natl Acad Sci U S A. 1995 May 9;92(10):4289-93.

48.

Kinesin-related proteins in eukaryotic flagella.

Fox LA, Sawin KE, Sale WS.

J Cell Sci. 1994 Jun;107 ( Pt 6):1545-50.

49.
50.

Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain.

Hogan CJ, Wein H, Wordeman L, Scholey JM, Sawin KE, Cande WZ.

Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6611-5.

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