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Items: 1 to 50 of 65

1.

Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii.

Gomez M, Leung W, Dantuluri S, Pillai A, Gani Z, Hwang S, McMillan LJ, Kiljunen S, Savilahti H, Maupin-Furlow JA.

Genes (Basel). 2018 Nov 20;9(11). pii: E562. doi: 10.3390/genes9110562.

2.

Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering.

Rasila TS, Pulkkinen E, Kiljunen S, Haapa-Paananen S, Pajunen MI, Salminen A, Paulin L, Vihinen M, Rice PA, Savilahti H.

Nucleic Acids Res. 2018 May 18;46(9):4649-4661. doi: 10.1093/nar/gkx1281.

3.

Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes.

Haapa-Paananen S, Savilahti H.

Methods Mol Biol. 2018;1681:279-286. doi: 10.1007/978-1-4939-7343-9_20.

PMID:
29134602
4.

Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants.

Legerme G, Yang E, Esquivel RN, Kiljunen S, Savilahti H, Pohlschroder M.

Life (Basel). 2016 Nov 26;6(4). pii: E41.

5.

Transposon Insertion Mutagenesis for Archaeal Gene Discovery.

Kiljunen S, Pajunen MI, Savilahti H.

Methods Mol Biol. 2017;1498:309-320.

PMID:
27709584
6.

A set of mini-Mu transposons for versatile cloning of circular DNA and novel dual-transposon strategy for increased efficiency.

Pulkkinen E, Haapa-Paananen S, Turakainen H, Savilahti H.

Plasmid. 2016 Jul;86:46-53. doi: 10.1016/j.plasmid.2016.07.002. Epub 2016 Jul 4.

PMID:
27387339
7.

MuA-mediated in vitro cloning of circular DNA: transpositional autointegration and the effect of MuB.

Pulkkinen E, Haapa-Paananen S, Savilahti H.

Mol Genet Genomics. 2016 Jun;291(3):1181-91. doi: 10.1007/s00438-016-1175-2. Epub 2016 Feb 4.

PMID:
26847688
8.

An assay to monitor the activity of DNA transposition complexes yields a general quality control measure for transpositional recombination reactions.

Pulkkinen E, Haapa-Paananen S, Savilahti H.

Mob Genet Elements. 2014 Oct 30;4(5):1-8. eCollection 2014 Oct.

9.

Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery.

Kiljunen S, Pajunen MI, Dilks K, Storf S, Pohlschroder M, Savilahti H.

BMC Biol. 2014 Dec 9;12:103. doi: 10.1186/s12915-014-0103-3.

10.

Phylogenetic analysis of Maverick/Polinton giant transposons across organisms.

Haapa-Paananen S, Wahlberg N, Savilahti H.

Mol Phylogenet Evol. 2014 Sep;78:271-4. doi: 10.1016/j.ympev.2014.05.024. Epub 2014 Jun 2.

PMID:
24882428
11.

Flexibility in MuA transposase family protein structures: functional mapping with scanning mutagenesis and sequence alignment of protein homologues.

Rasila TS, Vihinen M, Paulin L, Haapa-Paananen S, Savilahti H.

PLoS One. 2012;7(5):e37922. doi: 10.1371/journal.pone.0037922. Epub 2012 May 29.

12.

A method to sequence and quantify DNA integration for monitoring outcome in gene therapy.

Brady T, Roth SL, Malani N, Wang GP, Berry CC, Leboulch P, Hacein-Bey-Abina S, Cavazzana-Calvo M, Papapetrou EP, Sadelain M, Savilahti H, Bushman FD.

Nucleic Acids Res. 2011 Jun;39(11):e72. doi: 10.1093/nar/gkr140. Epub 2011 Mar 16.

13.

Missing-in-metastasis MIM/MTSS1 promotes actin assembly at intercellular junctions and is required for integrity of kidney epithelia.

Saarikangas J, Mattila PK, Varjosalo M, Bovellan M, Hakanen J, Calzada-Wack J, Tost M, Jennen L, Rathkolb B, Hans W, Horsch M, Hyvönen ME, Perälä N, Fuchs H, Gailus-Durner V, Esposito I, Wolf E, de Angelis MH, Frilander MJ, Savilahti H, Sariola H, Sainio K, Lehtonen S, Taipale J, Salminen M, Lappalainen P.

J Cell Sci. 2011 Apr 15;124(Pt 8):1245-55. doi: 10.1242/jcs.082610. Epub 2011 Mar 15.

14.

Two distinct regions in the model protein Peb1 are critical for its heterologous transport out of Escherichia coli.

Anton L, Majander K, Savilahti H, Laakkonen L, Westerlund-Wikström B.

Microb Cell Fact. 2010 Dec 2;9:97. doi: 10.1186/1475-2859-9-97.

15.

Universal platform for quantitative analysis of DNA transposition.

Pajunen MI, Rasila TS, Happonen LJ, Lamberg A, Haapa-Paananen S, Kiljunen S, Savilahti H.

Mob DNA. 2010 Nov 26;1(1):24. doi: 10.1186/1759-8753-1-24.

16.

Genetic recombination pathways and their application for genome modification of human embryonic stem cells.

Nieminen M, Tuuri T, Savilahti H.

Exp Cell Res. 2010 Oct 1;316(16):2578-86. doi: 10.1016/j.yexcr.2010.06.004. Epub 2010 Jun 11. Review.

PMID:
20542027
17.

Mso1p regulates membrane fusion through interactions with the putative N-peptide-binding area in Sec1p domain 1.

Weber M, Chernov K, Turakainen H, Wohlfahrt G, Pajunen M, Savilahti H, Jäntti J.

Mol Biol Cell. 2010 Apr 15;21(8):1362-74. doi: 10.1091/mbc.E09-07-0546. Epub 2010 Feb 24.

18.

Critical evaluation of random mutagenesis by error-prone polymerase chain reaction protocols, Escherichia coli mutator strain, and hydroxylamine treatment.

Rasila TS, Pajunen MI, Savilahti H.

Anal Biochem. 2009 May 1;388(1):71-80. doi: 10.1016/j.ab.2009.02.008. Epub 2009 Feb 10.

PMID:
19454214
19.

Application of Mu in vitro transposition for high-precision mapping of protein-protein interfaces on a yeast two-hybrid platform.

Pajunen M, Poussu E, Turakainen H, Savilahti H.

Methods. 2009 Nov;49(3):255-62. doi: 10.1016/j.ymeth.2009.04.014. Epub 2009 May 4.

PMID:
19410001
20.

Generation of single-copy transposon insertions in Clostridium perfringens by electroporation of phage mu DNA transposition complexes.

Lanckriet A, Timbermont L, Happonen LJ, Pajunen MI, Pasmans F, Haesebrouck F, Ducatelle R, Savilahti H, Van Immerseel F.

Appl Environ Microbiol. 2009 May;75(9):2638-42. doi: 10.1128/AEM.02214-08. Epub 2009 Mar 6.

21.

Transposition-based method for the rapid generation of gene-targeting vectors to produce Cre/Flp-modifiable conditional knock-out mice.

Turakainen H, Saarimäki-Vire J, Sinjushina N, Partanen J, Savilahti H.

PLoS One. 2009;4(2):e4341. doi: 10.1371/journal.pone.0004341. Epub 2009 Feb 5.

22.

Mu transposition complex mutagenesis in Lactococcus lactis--identification of genes affecting nisin production.

Wu Z, Xuanyuan Z, Li R, Jiang D, Li C, Xu H, Bai Y, Zhang X, Turakainen H, Saris PE, Savilahti H, Qiao M.

J Appl Microbiol. 2009 Jan;106(1):41-8. doi: 10.1111/j.1365-2672.2008.03962.x. Epub 2008 Dec 11.

23.

Bacteriophage Mu integration in yeast and mammalian genomes.

Paatero AO, Turakainen H, Happonen LJ, Olsson C, Palomäki T, Pajunen MI, Meng X, Otonkoski T, Tuuri T, Berry C, Malani N, Frilander MJ, Bushman FD, Savilahti H.

Nucleic Acids Res. 2008 Dec;36(22):e148. doi: 10.1093/nar/gkn801. Epub 2008 Oct 25.

24.
25.

SNP discovery by mismatch-targeting of Mu transposition.

Orsini L, Pajunen M, Hanski I, Savilahti H.

Nucleic Acids Res. 2007;35(6):e44. Epub 2007 Feb 20.

26.

Isolation and characterization of biofilm formation-defective mutants of Staphylococcus aureus.

Tu Quoc PH, Genevaux P, Pajunen M, Savilahti H, Georgopoulos C, Schrenzel J, Kelley WL.

Infect Immun. 2007 Mar;75(3):1079-88. Epub 2006 Dec 11.

27.

Genome characterization of lipid-containing marine bacteriophage PM2 by transposon insertion mutagenesis.

Krupovic M, Vilen H, Bamford JK, Kivelä HM, Aalto JM, Savilahti H, Bamford DH.

J Virol. 2006 Sep;80(18):9270-8.

28.

Characteristics of MuA transposase-catalyzed processing of model transposon end DNA hairpin substrates.

Saariaho AH, Savilahti H.

Nucleic Acids Res. 2006 Jun 6;34(10):3139-49. Print 2006.

29.

Meox1Cre: a mouse line expressing Cre recombinase in somitic mesoderm.

Jukkola T, Trokovic R, Maj P, Lamberg A, Mankoo B, Pachnis V, Savilahti H, Partanen J.

Genesis. 2005 Nov;43(3):148-53.

PMID:
16267823
30.

Actin-organising properties of the muscular dystrophy protein myotilin.

von Nandelstadh P, Grönholm M, Moza M, Lamberg A, Savilahti H, Carpén O.

Exp Cell Res. 2005 Oct 15;310(1):131-9.

PMID:
16122733
32.

Generation of transposon insertion mutant libraries for Gram-positive bacteria by electroporation of phage Mu DNA transposition complexes.

Pajunen MI, Pulliainen AT, Finne J, Savilahti H.

Microbiology. 2005 Apr;151(Pt 4):1209-18.

PMID:
15817788
33.

Behavioural phenotypes of hypomorphic KCC2-deficient mice.

Tornberg J, Voikar V, Savilahti H, Rauvala H, Airaksinen MS.

Eur J Neurosci. 2005 Mar;21(5):1327-37.

PMID:
15813942
34.

Nonessential genes of phage phiYeO3-12 include genes involved in adaptation to growth on Yersinia enterocolitica serotype O:3.

Kiljunen S, Vilen H, Pajunen M, Savilahti H, Skurnik M.

J Bacteriol. 2005 Feb;187(4):1405-14.

38.

Structure-function analysis of PrsA reveals roles for the parvulin-like and flanking N- and C-terminal domains in protein folding and secretion in Bacillus subtilis.

Vitikainen M, Lappalainen I, Seppala R, Antelmann H, Boer H, Taira S, Savilahti H, Hecker M, Vihinen M, Sarvas M, Kontinen VP.

J Biol Chem. 2004 Apr 30;279(18):19302-14. Epub 2004 Feb 19.

39.

Transposon mutagenesis of the phage phi YeO3-12.

Kiljunen S, Vilen H, Savilahti H, Skurnik M.

Adv Exp Med Biol. 2003;529:245-8. No abstract available.

PMID:
12756765
40.

Use of intein-directed peptide biosynthesis to improve serum stability and bioactivity of a gelatinase inhibitory peptide.

Björklund M, Valtanen H, Savilahti H, Koivunen E.

Comb Chem High Throughput Screen. 2003 Feb;6(1):29-35.

PMID:
12570750
41.

A direct transposon insertion tool for modification and functional analysis of viral genomes.

Vilen H, Aalto JM, Kassinen A, Paulin L, Savilahti H.

J Virol. 2003 Jan;77(1):123-34.

42.

Functional genomics on potato virus A: virus genome-wide map of sites essential for virus propagation.

Kekarainen T, Savilahti H, Valkonen JP.

Genome Res. 2002 Apr;12(4):584-94. Erratum in: Genome Res 2002 May;12(5):840.

43.
44.

DNA transposition of bacteriophage Mu. A quantitative analysis of target site selection in vitro.

Haapa-Paananen S, Rita H, Savilahti H.

J Biol Chem. 2002 Jan 25;277(4):2843-51. Epub 2001 Nov 7.

45.
46.

Functional characterization of the human immunodeficiency virus type 1 genome by genetic footprinting.

Laurent LC, Olsen MN, Crowley RA, Savilahti H, Brown PO.

J Virol. 2000 Mar;74(6):2760-9.

47.

Retrotransposon BARE-1: expression of encoded proteins and formation of virus-like particles in barley cells

Jaaskelainen M, Mykkanen AH, Arna T, Vicient CM, Suoniemi A, Kalendar R, Savilahti H, Schulman AH.

Plant J. 1999 Nov;20(4):413-22.

48.

Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene encoding Hrp pilus subunit.

Taira S, Tuimala J, Roine E, Nurmiaho-Lassila EL, Savilahti H, Romantschuk M.

Mol Microbiol. 1999 Nov;34(4):737-44.

50.

An efficient DNA sequencing strategy based on the bacteriophage mu in vitro DNA transposition reaction.

Haapa S, Suomalainen S, Eerikäinen S, Airaksinen M, Paulin L, Savilahti H.

Genome Res. 1999 Mar;9(3):308-15.

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