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Items: 1 to 50 of 478

1.

Ligation and Ligases.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Aug 1;2019(8):pdb.top101352. doi: 10.1101/pdb.top101352.

PMID:
31371476
2.

Attaching Phosphorylated Adaptors/Linkers to Blunt-Ended DNAs.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Aug 1;2019(8):pdb.prot101261. doi: 10.1101/pdb.prot101261.

PMID:
31371475
3.

Synthesis of cDNA Probes from mRNA Using Random Oligonucleotide Primers.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Jul 1;2019(7):pdb.prot100636. doi: 10.1101/pdb.prot100636.

PMID:
31262964
4.

Polymerase Chain Reaction.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Jun 3;2019(6):pdb.top095109. doi: 10.1101/pdb.top095109.

PMID:
31160389
5.

Screening Colonies by Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Jun 3;2019(6):pdb.prot095224. doi: 10.1101/pdb.prot095224.

PMID:
31160384
6.

Rapid Amplification of Sequences from the 3' Ends of mRNAs: 3'-RACE.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 May 1;2019(5):pdb.prot095216. doi: 10.1101/pdb.prot095216.

PMID:
31043557
7.

Rapid Amplification of Sequences from the 5' Ends of mRNAs: 5'-RACE.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 May 1;2019(5):pdb.prot095208. doi: 10.1101/pdb.prot095208.

PMID:
31043556
8.

Amplification of cDNA Generated by Reverse Transcription of mRNA: Two-Step Reverse Transcription-Polymerase Chain Reaction (RT-PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 May 1;2019(5):pdb.prot095190. doi: 10.1101/pdb.prot095190.

PMID:
31043555
9.

Measurement of Radioactivity in Nucleic Acids.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Mar 1;2019(3):pdb.prot100933. doi: 10.1101/pdb.prot100933.

PMID:
30824626
10.

Spun-Column Chromatography.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Mar 1;2019(3):pdb.prot100594. doi: 10.1101/pdb.prot100594.

PMID:
30824625
11.

Random Priming: Labeling of Purified DNA Fragments by Extension of Random Oligonucleotides.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Mar 1;2019(3):pdb.prot100586. doi: 10.1101/pdb.prot100586.

PMID:
30824624
12.

Long and Accurate Polymerase Chain Reaction (LA PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Mar 1;2019(3):pdb.prot095158. doi: 10.1101/pdb.prot095158.

PMID:
30824616
13.

How to Win the Battle with RNase.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.top101857. doi: 10.1101/pdb.top101857.

PMID:
30710029
14.

Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot100479. doi: 10.1101/pdb.prot100479.

PMID:
30710028
15.

Nested Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot095182. doi: 10.1101/pdb.prot095182.

PMID:
30710024
16.

Inverse Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot095166. doi: 10.1101/pdb.prot095166.

PMID:
30710023
17.

Polymerase Chain Reaction (PCR) Amplification of GC-Rich Templates.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot095141. doi: 10.1101/pdb.prot095141.

PMID:
30710022
18.

Analysis of DNA by Agarose Gel Electrophoresis.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Jan 2;2019(1):pdb.top100388. doi: 10.1101/pdb.top100388.

PMID:
30602561
19.

Agarose Gel Electrophoresis.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Jan 2;2019(1):pdb.prot100404. doi: 10.1101/pdb.prot100404.

PMID:
30602560
20.

Analysis and Normalization of Real-Time Polymerase Chain Reaction (PCR) Experimental Data.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Oct 1;2018(10). doi: 10.1101/pdb.top095000.

PMID:
30275081
21.

Quantification of RNA by Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Oct 1;2018(10). doi: 10.1101/pdb.prot095042.

PMID:
30275077
22.

Quantification of DNA by Real-Time Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Oct 1;2018(10). doi: 10.1101/pdb.prot095034.

PMID:
30275076
23.

Constructing a Standard Curve for Real-Time Polymerase Chain Reaction (PCR) Experiments.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Oct 1;2018(10). doi: 10.1101/pdb.prot095026.

PMID:
30275075
24.

Optimizing Primer and Probe Concentrations for Use in Real-Time Polymerase Chain Reaction (PCR) Assays.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Oct 1;2018(10). doi: 10.1101/pdb.prot095018.

PMID:
30275074
25.

Isolation and Quantification of DNA.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Jun 1;2018(6). doi: 10.1101/pdb.top093336.

PMID:
29858343
26.

Rapid Isolation of Yeast DNA.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Jun 1;2018(6). doi: 10.1101/pdb.prot093542.

PMID:
29858334
27.

Touchdown Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 May 1;2018(5). doi: 10.1101/pdb.prot095133.

PMID:
29717053
28.

Hot Start Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 May 1;2018(5). doi: 10.1101/pdb.prot095125.

PMID:
29717052
29.

The Basic Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 May 1;2018(5). doi: 10.1101/pdb.prot095117.

PMID:
29717051
30.

Isolation of DNA from Mouse Tails without Extraction by Organic Solvents.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 May 1;2018(5). doi: 10.1101/pdb.prot093526.

PMID:
29717047
31.

Isolation of High-Molecular-Weight DNA from Suspension Cultures of Mammalian Cells Using Proteinase K and Phenol.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Apr 2;2018(4). doi: 10.1101/pdb.prot093476.

PMID:
29610360
32.

The Hanahan Method for Preparation and Transformation of Competent Escherichia coli: High-Efficiency Transformation.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Mar 1;2018(3). doi: 10.1101/pdb.prot101188.

PMID:
29496820
33.

Buffers.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Feb 1;2018(2). doi: 10.1101/pdb.top098210.

PMID:
29438064
34.

Caring for Escherichia coli.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Feb 1;2018(2). doi: 10.1101/pdb.prot101337.

PMID:
29438063
35.

One-Tube Isolation of DNA from Mouse Tails.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Feb 1;2018(2). doi: 10.1101/pdb.prot093534.

PMID:
29438056
36.

A Single-Step Method for the Simultaneous Preparation of DNA, RNA, and Protein from Cells and Tissues.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Jan 2;2018(1). doi: 10.1101/pdb.prot093500.

PMID:
29295899
37.

Preparation of Plasmid DNA by Alkaline Lysis with Sodium Dodecyl Sulfate: Maxipreps.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Jan 2;2018(1). doi: 10.1101/pdb.prot093351.

PMID:
29295898
38.

Preparation of Double-Stranded (Replicative Form) Bacteriophage M13 DNA.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Nov 1;2017(11):pdb.prot093443. doi: 10.1101/pdb.prot093443.

PMID:
29093201
39.

Growing Bacteriophage M13 in Liquid Culture.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Nov 1;2017(11):pdb.prot093435. doi: 10.1101/pdb.prot093435.

PMID:
29093200
40.

Preparation of Single-Stranded Bacteriophage M13 DNA by Precipitation with Polyethylene Glycol.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Nov 1;2017(11):pdb.prot093419. doi: 10.1101/pdb.prot093419.

PMID:
29093199
41.

NOX1 loss-of-function genetic variants in patients with inflammatory bowel disease.

Schwerd T, Bryant RV, Pandey S, Capitani M, Meran L, Cazier JB, Jung J, Mondal K, Parkes M, Mathew CG, Fiedler K, McCarthy DJ; WGS500 Consortium; Oxford IBD cohort study investigators; COLORS in IBD group investigators; UK IBD Genetics Consortium, Sullivan PB, Rodrigues A, Travis SPL, Moore C, Sambrook J, Ouwehand WH, Roberts DJ, Danesh J; INTERVAL Study, Russell RK, Wilson DC, Kelsen JR, Cornall R, Denson LA, Kugathasan S, Knaus UG, Serra EG, Anderson CA, Duerr RH, McGovern DP, Cho J, Powrie F, Li VS, Muise AM, Uhlig HH.

Mucosal Immunol. 2018 Mar;11(2):562-574. doi: 10.1038/mi.2017.74. Epub 2017 Nov 1.

42.

Isolation of High-Molecular-Weight DNA from Mammalian Blood Using Proteinase K and Phenol.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Oct 3;2017(10):pdb.prot093492. doi: 10.1101/pdb.prot093492.

PMID:
28974655
43.

Plating Bacteriophage M13.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Oct 3;2017(10):pdb.prot093427. doi: 10.1101/pdb.prot093427.

PMID:
28974654
44.

Preparation of Genomic DNA from Mouse Tails and Other Small Samples.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Sep 1;2017(9):pdb.prot093518. doi: 10.1101/pdb.prot093518.

PMID:
28864567
45.

Precipitation of DNA with Isopropanol.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Aug 1;2017(8):pdb.prot093385. doi: 10.1101/pdb.prot093385.

PMID:
28765297
46.

Isolation of High-Molecular-Weight DNA from Monolayer Cultures of Mammalian Cells Using Proteinase K and Phenol.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Jul 5;2017(7):pdb.prot093468. doi: 10.1101/pdb.prot093468.

PMID:
28679704
47.

Concentrating Nucleic Acids by Extraction with Butanol.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Jun 1;2017(6):pdb.prot093401. doi: 10.1101/pdb.prot093401.

PMID:
28572186
48.

Estimating the Concentration of DNA by Fluorometry Using Hoechst 33258.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 May 1;2017(5). doi: 10.1101/pdb.prot093567.

PMID:
28461657
49.

Isolation of High-Molecular-Weight DNA Using Organic Solvents.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Apr 3;2017(4):pdb.prot093450. doi: 10.1101/pdb.prot093450.

PMID:
28373491
50.

Isolation of High-Molecular-Weight DNA from Mammalian Tissues Using Proteinase K and Phenol.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Mar 1;2017(3). doi: 10.1101/pdb.prot093484.

PMID:
28250215

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