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Items: 1 to 50 of 479

1.

Recovery of DNA from Agarose Gels Using Glass Beads.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Sep 3;2019(9):pdb.prot100453. doi: 10.1101/pdb.prot100453.

PMID:
31481493
2.

Ligation and Ligases.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Aug 1;2019(8):pdb.top101352. doi: 10.1101/pdb.top101352.

PMID:
31371476
3.

Attaching Phosphorylated Adaptors/Linkers to Blunt-Ended DNAs.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Aug 1;2019(8):pdb.prot101261. doi: 10.1101/pdb.prot101261.

PMID:
31371475
4.

Synthesis of cDNA Probes from mRNA Using Random Oligonucleotide Primers.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Jul 1;2019(7):pdb.prot100636. doi: 10.1101/pdb.prot100636.

PMID:
31262964
5.

Polymerase Chain Reaction.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Jun 3;2019(6):pdb.top095109. doi: 10.1101/pdb.top095109.

PMID:
31160389
6.

Screening Colonies by Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Jun 3;2019(6):pdb.prot095224. doi: 10.1101/pdb.prot095224.

PMID:
31160384
7.

Rapid Amplification of Sequences from the 3' Ends of mRNAs: 3'-RACE.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 May 1;2019(5):pdb.prot095216. doi: 10.1101/pdb.prot095216.

PMID:
31043557
8.

Rapid Amplification of Sequences from the 5' Ends of mRNAs: 5'-RACE.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 May 1;2019(5):pdb.prot095208. doi: 10.1101/pdb.prot095208.

PMID:
31043556
9.

Amplification of cDNA Generated by Reverse Transcription of mRNA: Two-Step Reverse Transcription-Polymerase Chain Reaction (RT-PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 May 1;2019(5):pdb.prot095190. doi: 10.1101/pdb.prot095190.

PMID:
31043555
10.

Measurement of Radioactivity in Nucleic Acids.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Mar 1;2019(3):pdb.prot100933. doi: 10.1101/pdb.prot100933.

PMID:
30824626
11.

Spun-Column Chromatography.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Mar 1;2019(3):pdb.prot100594. doi: 10.1101/pdb.prot100594.

PMID:
30824625
12.

Random Priming: Labeling of Purified DNA Fragments by Extension of Random Oligonucleotides.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Mar 1;2019(3):pdb.prot100586. doi: 10.1101/pdb.prot100586.

PMID:
30824624
13.

Long and Accurate Polymerase Chain Reaction (LA PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Mar 1;2019(3):pdb.prot095158. doi: 10.1101/pdb.prot095158.

PMID:
30824616
14.

How to Win the Battle with RNase.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.top101857. doi: 10.1101/pdb.top101857.

PMID:
30710029
15.

Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot100479. doi: 10.1101/pdb.prot100479.

PMID:
30710028
16.

Nested Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot095182. doi: 10.1101/pdb.prot095182.

PMID:
30710024
17.

Inverse Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot095166. doi: 10.1101/pdb.prot095166.

PMID:
30710023
18.

Polymerase Chain Reaction (PCR) Amplification of GC-Rich Templates.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot095141. doi: 10.1101/pdb.prot095141.

PMID:
30710022
19.

Analysis of DNA by Agarose Gel Electrophoresis.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Jan 2;2019(1):pdb.top100388. doi: 10.1101/pdb.top100388.

PMID:
30602561
20.

Agarose Gel Electrophoresis.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2019 Jan 2;2019(1):pdb.prot100404. doi: 10.1101/pdb.prot100404.

PMID:
30602560
21.

Analysis and Normalization of Real-Time Polymerase Chain Reaction (PCR) Experimental Data.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Oct 1;2018(10). doi: 10.1101/pdb.top095000.

PMID:
30275081
22.

Quantification of RNA by Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Oct 1;2018(10). doi: 10.1101/pdb.prot095042.

PMID:
30275077
23.

Quantification of DNA by Real-Time Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Oct 1;2018(10). doi: 10.1101/pdb.prot095034.

PMID:
30275076
24.

Constructing a Standard Curve for Real-Time Polymerase Chain Reaction (PCR) Experiments.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Oct 1;2018(10). doi: 10.1101/pdb.prot095026.

PMID:
30275075
25.

Optimizing Primer and Probe Concentrations for Use in Real-Time Polymerase Chain Reaction (PCR) Assays.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Oct 1;2018(10). doi: 10.1101/pdb.prot095018.

PMID:
30275074
26.

Isolation and Quantification of DNA.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Jun 1;2018(6). doi: 10.1101/pdb.top093336.

PMID:
29858343
27.

Rapid Isolation of Yeast DNA.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Jun 1;2018(6). doi: 10.1101/pdb.prot093542.

PMID:
29858334
28.

Touchdown Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 May 1;2018(5). doi: 10.1101/pdb.prot095133.

PMID:
29717053
29.

Hot Start Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 May 1;2018(5). doi: 10.1101/pdb.prot095125.

PMID:
29717052
30.

The Basic Polymerase Chain Reaction (PCR).

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 May 1;2018(5). doi: 10.1101/pdb.prot095117.

PMID:
29717051
31.

Isolation of DNA from Mouse Tails without Extraction by Organic Solvents.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 May 1;2018(5). doi: 10.1101/pdb.prot093526.

PMID:
29717047
32.

Isolation of High-Molecular-Weight DNA from Suspension Cultures of Mammalian Cells Using Proteinase K and Phenol.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Apr 2;2018(4). doi: 10.1101/pdb.prot093476.

PMID:
29610360
33.

The Hanahan Method for Preparation and Transformation of Competent Escherichia coli: High-Efficiency Transformation.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Mar 1;2018(3). doi: 10.1101/pdb.prot101188.

PMID:
29496820
34.

Buffers.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Feb 1;2018(2). doi: 10.1101/pdb.top098210.

PMID:
29438064
35.

Caring for Escherichia coli.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Feb 1;2018(2). doi: 10.1101/pdb.prot101337.

PMID:
29438063
36.

One-Tube Isolation of DNA from Mouse Tails.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Feb 1;2018(2). doi: 10.1101/pdb.prot093534.

PMID:
29438056
37.

A Single-Step Method for the Simultaneous Preparation of DNA, RNA, and Protein from Cells and Tissues.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Jan 2;2018(1). doi: 10.1101/pdb.prot093500.

PMID:
29295899
38.

Preparation of Plasmid DNA by Alkaline Lysis with Sodium Dodecyl Sulfate: Maxipreps.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2018 Jan 2;2018(1). doi: 10.1101/pdb.prot093351.

PMID:
29295898
39.

Preparation of Double-Stranded (Replicative Form) Bacteriophage M13 DNA.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Nov 1;2017(11):pdb.prot093443. doi: 10.1101/pdb.prot093443.

PMID:
29093201
40.

Growing Bacteriophage M13 in Liquid Culture.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Nov 1;2017(11):pdb.prot093435. doi: 10.1101/pdb.prot093435.

PMID:
29093200
41.

Preparation of Single-Stranded Bacteriophage M13 DNA by Precipitation with Polyethylene Glycol.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Nov 1;2017(11):pdb.prot093419. doi: 10.1101/pdb.prot093419.

PMID:
29093199
42.

NOX1 loss-of-function genetic variants in patients with inflammatory bowel disease.

Schwerd T, Bryant RV, Pandey S, Capitani M, Meran L, Cazier JB, Jung J, Mondal K, Parkes M, Mathew CG, Fiedler K, McCarthy DJ; WGS500 Consortium; Oxford IBD cohort study investigators; COLORS in IBD group investigators; UK IBD Genetics Consortium, Sullivan PB, Rodrigues A, Travis SPL, Moore C, Sambrook J, Ouwehand WH, Roberts DJ, Danesh J; INTERVAL Study, Russell RK, Wilson DC, Kelsen JR, Cornall R, Denson LA, Kugathasan S, Knaus UG, Serra EG, Anderson CA, Duerr RH, McGovern DP, Cho J, Powrie F, Li VS, Muise AM, Uhlig HH.

Mucosal Immunol. 2018 Mar;11(2):562-574. doi: 10.1038/mi.2017.74. Epub 2017 Nov 1.

43.

Isolation of High-Molecular-Weight DNA from Mammalian Blood Using Proteinase K and Phenol.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Oct 3;2017(10):pdb.prot093492. doi: 10.1101/pdb.prot093492.

PMID:
28974655
44.

Plating Bacteriophage M13.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Oct 3;2017(10):pdb.prot093427. doi: 10.1101/pdb.prot093427.

PMID:
28974654
45.

Preparation of Genomic DNA from Mouse Tails and Other Small Samples.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Sep 1;2017(9):pdb.prot093518. doi: 10.1101/pdb.prot093518.

PMID:
28864567
46.

Precipitation of DNA with Isopropanol.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Aug 1;2017(8):pdb.prot093385. doi: 10.1101/pdb.prot093385.

PMID:
28765297
47.

Isolation of High-Molecular-Weight DNA from Monolayer Cultures of Mammalian Cells Using Proteinase K and Phenol.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Jul 5;2017(7):pdb.prot093468. doi: 10.1101/pdb.prot093468.

PMID:
28679704
48.

Concentrating Nucleic Acids by Extraction with Butanol.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Jun 1;2017(6):pdb.prot093401. doi: 10.1101/pdb.prot093401.

PMID:
28572186
49.

Estimating the Concentration of DNA by Fluorometry Using Hoechst 33258.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 May 1;2017(5). doi: 10.1101/pdb.prot093567.

PMID:
28461657
50.

Isolation of High-Molecular-Weight DNA Using Organic Solvents.

Green MR, Sambrook J.

Cold Spring Harb Protoc. 2017 Apr 3;2017(4):pdb.prot093450. doi: 10.1101/pdb.prot093450.

PMID:
28373491

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