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Items: 35


Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system.

Gregor C, Pape JK, Gwosch KC, Gilat T, Sahl SJ, Hell SW.

Proc Natl Acad Sci U S A. 2019 Dec 2. pii: 201913616. doi: 10.1073/pnas.1913616116. [Epub ahead of print]


Superresolution Fluorescence Imaging of Mutant Huntingtin Aggregation in Cells.

Sahl SJ, Vonk WIM.

Methods Mol Biol. 2019;1873:241-251. doi: 10.1007/978-1-4939-8820-4_15.


Robust nanoscopy of a synaptic protein in living mice by organic-fluorophore labeling.

Masch JM, Steffens H, Fischer J, Engelhardt J, Hubrich J, Keller-Findeisen J, D'Este E, Urban NT, Grant SGN, Sahl SJ, Kamin D, Hell SW.

Proc Natl Acad Sci U S A. 2018 Aug 21;115(34):E8047-E8056. doi: 10.1073/pnas.1807104115. Epub 2018 Aug 6.


Novel reversibly switchable fluorescent proteins for RESOLFT and STED nanoscopy engineered from the bacterial photoreceptor YtvA.

Gregor C, Sidenstein SC, Andresen M, Sahl SJ, Danzl JG, Hell SW.

Sci Rep. 2018 Feb 9;8(1):2724. doi: 10.1038/s41598-018-19947-1.


Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging.

Gregor C, Gwosch KC, Sahl SJ, Hell SW.

Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16.


A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes.

Bersuker K, Peterson CWH, To M, Sahl SJ, Savikhin V, Grossman EA, Nomura DK, Olzmann JA.

Dev Cell. 2018 Jan 8;44(1):97-112.e7. doi: 10.1016/j.devcel.2017.11.020. Epub 2017 Dec 21.


SRpHi ratiometric pH biosensors for super-resolution microscopy.

Richardson DS, Gregor C, Winter FR, Urban NT, Sahl SJ, Willig KI, Hell SW.

Nat Commun. 2017 Sep 18;8(1):577. doi: 10.1038/s41467-017-00606-4.


Photobleaching in STED nanoscopy and its dependence on the photon flux applied for reversible silencing of the fluorophore.

Oracz J, Westphal V, Radzewicz C, Sahl SJ, Hell SW.

Sci Rep. 2017 Sep 12;7(1):11354. doi: 10.1038/s41598-017-09902-x.


Fluorescence nanoscopy in cell biology.

Sahl SJ, Hell SW, Jakobs S.

Nat Rev Mol Cell Biol. 2017 Nov;18(11):685-701. doi: 10.1038/nrm.2017.71. Epub 2017 Sep 6. Review.


Adaptive-illumination STED nanoscopy.

Heine J, Reuss M, Harke B, D'Este E, Sahl SJ, Hell SW.

Proc Natl Acad Sci U S A. 2017 Sep 12;114(37):9797-9802. doi: 10.1073/pnas.1708304114. Epub 2017 Aug 28.


Multicolour nanoscopy of fixed and living cells with a single STED beam and hyperspectral detection.

Winter FR, Loidolt M, Westphal V, Butkevich AN, Gregor C, Sahl SJ, Hell SW.

Sci Rep. 2017 Apr 18;7:46492. doi: 10.1038/srep46492.


Achromatic light patterning and improved image reconstruction for parallelized RESOLFT nanoscopy.

Chmyrov A, Leutenegger M, Grotjohann T, Schönle A, Keller-Findeisen J, Kastrup L, Jakobs S, Donnert G, Sahl SJ, Hell SW.

Sci Rep. 2017 Mar 20;7:44619. doi: 10.1038/srep44619.


Ground State Depletion Nanoscopy Resolves Semiconductor Nanowire Barcode Segments at Room Temperature.

Oracz J, Adolfsson K, Westphal V, Radzewicz C, Borgström MT, Sahl SJ, Prinz CN, Hell SW.

Nano Lett. 2017 Apr 12;17(4):2652-2659. doi: 10.1021/acs.nanolett.7b00468. Epub 2017 Mar 9.


Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent.

Göttfert F, Pleiner T, Heine J, Westphal V, Görlich D, Sahl SJ, Hell SW.

Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):2125-2130. doi: 10.1073/pnas.1621495114. Epub 2017 Feb 13.


Comment on "Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics".

Sahl SJ, Balzarotti F, Keller-Findeisen J, Leutenegger M, Westphal V, Egner A, Lavoie-Cardinal F, Chmyrov A, Grotjohann T, Jakobs S.

Science. 2016 Apr 29;352(6285):527. doi: 10.1126/science.aad7983. Epub 2016 Apr 28.


Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT.

Hoyer P, de Medeiros G, Balázs B, Norlin N, Besir C, Hanne J, Kräusslich HG, Engelhardt J, Sahl SJ, Hell SW, Hufnagel L.

Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):3442-6. doi: 10.1073/pnas.1522292113. Epub 2016 Mar 16.


isoSTED nanoscopy with intrinsic beam alignment.

Curdt F, Herr SJ, Lutz T, Schmidt R, Engelhardt J, Sahl SJ, Hell SW.

Opt Express. 2015 Nov 30;23(24):30891-903. doi: 10.1364/OE.23.030891.


Delayed emergence of subdiffraction-sized mutant huntingtin fibrils following inclusion body formation.

Sahl SJ, Lau L, Vonk WI, Weiss LE, Frydman J, Moerner WE.

Q Rev Biophys. 2016 Jan;49:e2. Epub 2015 Sep 9.


Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics.

Schneider J, Zahn J, Maglione M, Sigrist SJ, Marquard J, Chojnacki J, Kräusslich HG, Sahl SJ, Engelhardt J, Hell SW.

Nat Methods. 2015 Sep;12(9):827-30. doi: 10.1038/nmeth.3481. Epub 2015 Jul 27.


Single-molecule imaging of Hedgehog pathway protein Smoothened in primary cilia reveals binding events regulated by Patched1.

Milenkovic L, Weiss LE, Yoon J, Roth TL, Su YS, Sahl SJ, Scott MP, Moerner WE.

Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8320-5. doi: 10.1073/pnas.1510094112. Epub 2015 Jun 22.


Lens-based fluorescence nanoscopy.

Eggeling C, Willig KI, Sahl SJ, Hell SW.

Q Rev Biophys. 2015 May;48(2):178-243. doi: 10.1017/S0033583514000146. Review.


STED nanoscopy with fluorescent quantum dots.

Hanne J, Falk HJ, Görlitz F, Hoyer P, Engelhardt J, Sahl SJ, Hell SW.

Nat Commun. 2015 May 18;6:7127. doi: 10.1038/ncomms8127.


Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions.

Shechtman Y, Weiss LE, Backer AS, Sahl SJ, Moerner WE.

Nano Lett. 2015 Jun 10;15(6):4194-9. doi: 10.1021/acs.nanolett.5b01396. Epub 2015 May 18.


2000-fold parallelized dual-color STED fluorescence nanoscopy.

Bergermann F, Alber L, Sahl SJ, Engelhardt J, Hell SW.

Opt Express. 2015 Jan 12;23(1):211-23. doi: 10.1364/OE.23.000211.


Optimal point spread function design for 3D imaging.

Shechtman Y, Sahl SJ, Backer AS, Moerner WE.

Phys Rev Lett. 2014 Sep 26;113(13):133902. Epub 2014 Sep 26.


A bisected pupil for studying single-molecule orientational dynamics and its application to three-dimensional super-resolution microscopy.

Backer AS, Backlund MP, von Diezmann AR, Sahl SJ, Moerner WE.

Appl Phys Lett. 2014 May 12;104(19):193701.


High-resolution tracking of single-molecule diffusion in membranes by confocalized and spatially differentiated fluorescence photon stream recording.

Sahl SJ, Leutenegger M, Hell SW, Eggeling C.

Chemphyschem. 2014 Mar 17;15(4):771-83. doi: 10.1002/cphc.201301090. Epub 2014 Mar 5.


The role of molecular dipole orientation in single-molecule fluorescence microscopy and implications for super-resolution imaging.

Backlund MP, Lew MD, Backer AS, Sahl SJ, Moerner WE.

Chemphyschem. 2014 Mar 17;15(4):587-99. doi: 10.1002/cphc.201300880. Epub 2013 Dec 30. Review.


Super-resolution fluorescence imaging with single molecules.

Sahl SJ, Moerner WE.

Curr Opin Struct Biol. 2013 Oct;23(5):778-87. doi: 10.1016/ Epub 2013 Aug 8. Review.


The double-helix point spread function enables precise and accurate measurement of 3D single-molecule localization and orientation.

Backlund MP, Lew MD, Backer AS, Sahl SJ, Grover G, Agrawal A, Piestun R, Moerner WE.

Proc SPIE Int Soc Opt Eng. 2013 Feb 22;8590:85900.


Cellular inclusion bodies of mutant huntingtin exon 1 obscure small fibrillar aggregate species.

Sahl SJ, Weiss LE, Duim WC, Frydman J, Moerner WE.

Sci Rep. 2012;2:895. doi: 10.1038/srep00895. Epub 2012 Nov 28.


Simultaneous, accurate measurement of the 3D position and orientation of single molecules.

Backlund MP, Lew MD, Backer AS, Sahl SJ, Grover G, Agrawal A, Piestun R, Moerner WE.

Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19087-92. doi: 10.1073/pnas.1216687109. Epub 2012 Nov 5.


STED microscopy with optimized labeling density reveals 9-fold arrangement of a centriole protein.

Lau L, Lee YL, Sahl SJ, Stearns T, Moerner WE.

Biophys J. 2012 Jun 20;102(12):2926-35. doi: 10.1016/j.bpj.2012.05.015. Epub 2012 Jun 19.


Fast molecular tracking maps nanoscale dynamics of plasma membrane lipids.

Sahl SJ, Leutenegger M, Hilbert M, Hell SW, Eggeling C.

Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6829-34. doi: 10.1073/pnas.0912894107. Epub 2010 Mar 29.

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