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Nat Commun. 2018 Oct 12;9(1):4252. doi: 10.1038/s41467-018-06453-1.

An expression atlas of variant ionotropic glutamate receptors identifies a molecular basis of carbonation sensing.

Author information

1
Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Génopode Building, Lausanne, CH-1015, Switzerland.
2
Centre for Neural Circuits and Behaviour, University of Oxford, Tinsley Building, Mansfield Road, Oxford, OX1 3SR, United Kingdom.
3
Department of Neurobiology and Behavior, Cornell University, W153 Mudd Hall, Ithaca, NY, 14853, USA.
4
Champalimaud Centre for the Unknown, Lisbon, 1400-038, Portugal.
5
Department of Biology, Institute of Zoology, University of Fribourg, Chemin du Musée 10, Fribourg, CH-1700, Switzerland.
6
Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Génopode Building, Lausanne, CH-1015, Switzerland. Richard.Benton@unil.ch.

Abstract

Through analysis of the Drosophila ionotropic receptors (IRs), a family of variant ionotropic glutamate receptors, we reveal that most IRs are expressed in peripheral neuron populations in diverse gustatory organs in larvae and adults. We characterise IR56d, which defines two anatomically-distinct neuron classes in the proboscis: one responds to carbonated solutions and fatty acids while the other represents a subset of sugar- and fatty acid-sensing cells. Mutational analysis indicates that IR56d, together with the broadly-expressed co-receptors IR25a and IR76b, is essential for physiological responses to carbonation and fatty acids, but not sugars. We further demonstrate that carbonation and fatty acids both promote IR56d-dependent attraction of flies, but through different behavioural outputs. Our work provides a toolkit for investigating taste functions of IRs, defines a subset of these receptors required for carbonation sensing, and illustrates how the gustatory system uses combinatorial expression of sensory molecules in distinct neurons to coordinate behaviour.

PMID:
30315166
PMCID:
PMC6185939
DOI:
10.1038/s41467-018-06453-1
[Indexed for MEDLINE]
Free PMC Article

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