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FRET-based binding assay between a fluorescent cAMP analogue and a cyclic nucleotide-binding domain tagged with a CFP.

Romero F, Santana-Calvo C, Sánchez-Guevara Y, Nishigaki T.

FEBS Lett. 2017 Sep;591(18):2869-2878. doi: 10.1002/1873-3468.12760. Epub 2017 Aug 20.


FRET analysis using sperm-activating peptides tagged with fluorescent proteins reveals that ligand-binding sites exist as clusters.

Arcos-Hernández C, Romero F, Sánchez-Guevara Y, Beltrán C, Nishigaki T.

J Exp Biol. 2016 Feb;219(Pt 4):508-15. doi: 10.1242/jeb.127662.


Measuring intracellular Ca2+ changes in human sperm using four techniques: conventional fluorometry, stopped flow fluorometry, flow cytometry and single cell imaging.

Mata-Martínez E, José O, Torres-Rodríguez P, Solís-López A, Sánchez-Tusie AA, Sánchez-Guevara Y, Treviño MB, Treviño CL.

J Vis Exp. 2013 May 24;(75):e50344. doi: 10.3791/50344.

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