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Plant Physiol. 2019 Nov 7. pii: pp.00740.2019. doi: 10.1104/pp.19.00740. [Epub ahead of print]

High flux through the oxidative pentose phosphate pathway lowers efficiency in developing Camelina seeds.

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Michigan State University CITY: E. Lansing STATE: MI United States Of America [US].
Brookhaven National Lab CITY: Upton STATE: NY POSTAL_CODE: 11973 United States Of America [US].
Greenwood Genetic Center CITY: Greenwood STATE: South Carolina United States Of America [US].
University of North Texas CITY: Denton STATE: Texas United States Of America [US].
Hartnell College CITY: Salinas STATE: California United States Of America [US].
Michigan State University CITY: East Lansing STATE: Michigan POSTAL_CODE: 48824-1312 United States Of America [US]


Many seeds are green during development, and light has been shown to play a role in the efficiency with which maternally supplied substrates are converted into storage compounds. However, the effects of light on the fluxes through central metabolism that determine this efficiency are poorly understood. Here, we used metabolic flux analysis to determine the effects of light on central metabolism in developing embryos of false flax (Camelina sativa). Metabolic efficiency in C. sativa is of interest because, despite its growing importance as a model oilseed and engineering target and its potential as a biofuel crop, its yields are lower than other major oilseed species. Culture conditions under which steady-state growth and composition of developing embryos match those in planta were used to quantify substrate uptake and respiration rates. The carbon conversion efficiency (CCE) was 21{plus minus}3% in the dark and 42{plus minus}4% under high light. Under physiological illumination, the CCE (32{plus minus}2%) was substantially lower than in green and non-green oilseeds studied previously. 13C and 14C isotopic labeling experiments were used together with computer-aided modelling to map fluxes through central metabolism. Fluxes through the oxidative pentose phosphate pathway (OPPP) were the principal source of CO2 production and strongly negatively correlated with CCE across light levels. OPPP fluxes were greatly in excess of demand for NAD(P)H for biosynthesis and larger than those measured in other systems. Excess reductant appears to be dissipated via cyanide-insensitive respiration. OPPP enzymes therefore represent a potential target for increasing efficiency and yield in C. sativa.


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