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Appl Environ Microbiol. 2012 Oct;78(19):7137-40. doi: 10.1128/AEM.01188-12. Epub 2012 Jul 20.

Regulatable vectors for environmental gene expression in Alphaproteobacteria.

Author information

1
Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, United Kingdom.

Abstract

Two expression vectors utilizing the inducible taurine promoter (tauAp) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment and in planta. The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins.

PMID:
22820336
PMCID:
PMC3457481
DOI:
10.1128/AEM.01188-12
[Indexed for MEDLINE]
Free PMC Article

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