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Items: 1 to 50 of 146

1.

Interactions of nuclear transport factors and surface-conjugated FG nucleoporins: Insights and limitations.

Hayama R, Sorci M, Keating Iv JJ, Hecht LM, Plawsky JL, Belfort G, Chait BT, Rout MP.

PLoS One. 2019 Jun 6;14(6):e0217897. doi: 10.1371/journal.pone.0217897. eCollection 2019.

2.

Principles for Integrative Structural Biology Studies.

Rout MP, Sali A.

Cell. 2019 May 30;177(6):1384-1403. doi: 10.1016/j.cell.2019.05.016. Review.

PMID:
31150619
3.

Pore timing: the evolutionary origins of the nucleus and nuclear pore complex.

Field MC, Rout MP.

F1000Res. 2019 Apr 3;8. pii: F1000 Faculty Rev-369. doi: 10.12688/f1000research.16402.1. eCollection 2019. Review.

4.

Günter Blobel 1936-2018.

Chook YM, Fontoura BMA, Rout MP.

Nat Struct Mol Biol. 2018 Apr;25(4):297-298. doi: 10.1038/s41594-018-0053-5. No abstract available.

PMID:
29610492
5.

Integrative structure and functional anatomy of a nuclear pore complex.

Kim SJ, Fernandez-Martinez J, Nudelman I, Shi Y, Zhang W, Raveh B, Herricks T, Slaughter BD, Hogan JA, Upla P, Chemmama IE, Pellarin R, Echeverria I, Shivaraju M, Chaudhury AS, Wang J, Williams R, Unruh JR, Greenberg CH, Jacobs EY, Yu Z, de la Cruz MJ, Mironska R, Stokes DL, Aitchison JD, Jarrold MF, Gerton JL, Ludtke SJ, Akey CW, Chait BT, Sali A, Rout MP.

Nature. 2018 Mar 22;555(7697):475-482. doi: 10.1038/nature26003. Epub 2018 Mar 14.

6.

Involvement in surface antigen expression by a moonlighting FG-repeat nucleoporin in trypanosomes.

Holden JM, Koreny L, Obado S, Ratushny AV, Chen WM, Bart JM, Navarro M, Chait BT, Aitchison JD, Rout MP, Field MC.

Mol Biol Cell. 2018 May 1;29(9):1100-1110. doi: 10.1091/mbc.E17-06-0430.

7.

Deciphering the "Fuzzy" Interaction of FG Nucleoporins and Transport Factors Using Small-Angle Neutron Scattering.

Sparks S, Temel DB, Rout MP, Cowburn D.

Structure. 2018 Mar 6;26(3):477-484.e4. doi: 10.1016/j.str.2018.01.010. Epub 2018 Feb 8.

8.

Thermodynamic characterization of the multivalent interactions underlying rapid and selective translocation through the nuclear pore complex.

Hayama R, Sparks S, Hecht LM, Dutta K, Karp JM, Cabana CM, Rout MP, Cowburn D.

J Biol Chem. 2018 Mar 23;293(12):4555-4563. doi: 10.1074/jbc.AC117.001649. Epub 2018 Jan 26.

9.

Dissection of affinity captured LINE-1 macromolecular complexes.

Taylor MS, Altukhov I, Molloy KR, Mita P, Jiang H, Adney EM, Wudzinska A, Badri S, Ischenko D, Eng G, Burns KH, Fenyö D, Chait BT, Alexeev D, Rout MP, Boeke JD, LaCava J.

Elife. 2018 Jan 8;7. pii: e30094. doi: 10.7554/eLife.30094.

10.

The nuclear pore complex core scaffold and permeability barrier: variations of a common theme.

Hayama R, Rout MP, Fernandez-Martinez J.

Curr Opin Cell Biol. 2017 Jun;46:110-118. doi: 10.1016/j.ceb.2017.05.003. Epub 2017 Jun 15. Review.

11.

The Evolution of Organellar Coat Complexes and Organization of the Eukaryotic Cell.

Rout MP, Field MC.

Annu Rev Biochem. 2017 Jun 20;86:637-657. doi: 10.1146/annurev-biochem-061516-044643. Epub 2017 May 3. Review.

PMID:
28471691
12.

Comparative interactomics provides evidence for functional specialization of the nuclear pore complex.

Obado SO, Field MC, Rout MP.

Nucleus. 2017 Jul 4;8(4):340-352. doi: 10.1080/19491034.2017.1313936. Epub 2017 May 2.

13.

Specialising the parasite nucleus: Pores, lamins, chromatin, and diversity.

Rout MP, Obado SO, Schenkman S, Field MC.

PLoS Pathog. 2017 Mar 2;13(3):e1006170. doi: 10.1371/journal.ppat.1006170. eCollection 2017 Mar. Review. No abstract available.

14.

Lineage-specific proteins essential for endocytosis in trypanosomes.

Manna PT, Obado SO, Boehm C, Gadelha C, Sali A, Chait BT, Rout MP, Field MC.

J Cell Sci. 2017 Apr 15;130(8):1379-1392. doi: 10.1242/jcs.191478. Epub 2017 Feb 23.

15.

Molecular Architecture of the Major Membrane Ring Component of the Nuclear Pore Complex.

Upla P, Kim SJ, Sampathkumar P, Dutta K, Cahill SM, Chemmama IE, Williams R, Bonanno JB, Rice WJ, Stokes DL, Cowburn D, Almo SC, Sali A, Rout MP, Fernandez-Martinez J.

Structure. 2017 Mar 7;25(3):434-445. doi: 10.1016/j.str.2017.01.006. Epub 2017 Feb 2.

16.

The Trypanosome Exocyst: A Conserved Structure Revealing a New Role in Endocytosis.

Boehm CM, Obado S, Gadelha C, Kaupisch A, Manna PT, Gould GW, Munson M, Chait BT, Rout MP, Field MC.

PLoS Pathog. 2017 Jan 23;13(1):e1006063. doi: 10.1371/journal.ppat.1006063. eCollection 2017 Jan.

17.

Protein Complex Affinity Capture from Cryomilled Mammalian Cells.

LaCava J, Jiang H, Rout MP.

J Vis Exp. 2016 Dec 9;(118). doi: 10.3791/54518.

18.

Structure and Function of the Nuclear Pore Complex Cytoplasmic mRNA Export Platform.

Fernandez-Martinez J, Kim SJ, Shi Y, Upla P, Pellarin R, Gagnon M, Chemmama IE, Wang J, Nudelman I, Zhang W, Williams R, Rice WJ, Stokes DL, Zenklusen D, Chait BT, Sali A, Rout MP.

Cell. 2016 Nov 17;167(5):1215-1228.e25. doi: 10.1016/j.cell.2016.10.028. Epub 2016 Nov 10.

19.

Simple rules for passive diffusion through the nuclear pore complex.

Timney BL, Raveh B, Mironska R, Trivedi JM, Kim SJ, Russel D, Wente SR, Sali A, Rout MP.

J Cell Biol. 2016 Oct 10;215(1):57-76. Epub 2016 Oct 3.

20.

Co-dependence between trypanosome nuclear lamina components in nuclear stability and control of gene expression.

Maishman L, Obado SO, Alsford S, Bart JM, Chen WM, Ratushny AV, Navarro M, Horn D, Aitchison JD, Chait BT, Rout MP, Field MC.

Nucleic Acids Res. 2016 Dec 15;44(22):10554-10570. Epub 2016 Sep 12.

21.

Cilia and Nuclear Pore Proteins: Pore No More?

Obado SO, Rout MP.

Dev Cell. 2016 Sep 12;38(5):445-6. doi: 10.1016/j.devcel.2016.08.019.

22.

Purification and analysis of endogenous human RNA exosome complexes.

Domanski M, Upla P, Rice WJ, Molloy KR, Ketaren NE, Stokes DL, Jensen TH, Rout MP, LaCava J.

RNA. 2016 Sep;22(9):1467-75. doi: 10.1261/rna.057760.116. Epub 2016 Jul 11.

23.

HIV-host interactome revealed directly from infected cells.

Luo Y, Jacobs EY, Greco TM, Mohammed KD, Tong T, Keegan S, Binley JM, Cristea IM, Fenyö D, Rout MP, Chait BT, Muesing MA.

Nat Microbiol. 2016 Jul;1(7):16068. Epub 2016 May 23.

24.

Protein Complex Purification by Affinity Capture.

LaCava J, Fernandez-Martinez J, Hakhverdyan Z, Rout MP.

Cold Spring Harb Protoc. 2016 Jul 1;2016(7). doi: 10.1101/pdb.top077545.

PMID:
27371601
25.

Density Gradient Ultracentrifugation to Isolate Endogenous Protein Complexes after Affinity Capture.

Fernandez-Martinez J, LaCava J, Rout MP.

Cold Spring Harb Protoc. 2016 Jul 1;2016(7). doi: 10.1101/pdb.prot087957.

PMID:
27371598
26.

Native Elution of Yeast Protein Complexes Obtained by Affinity Capture.

LaCava J, Fernandez-Martinez J, Rout MP.

Cold Spring Harb Protoc. 2016 Jul 1;2016(7). doi: 10.1101/pdb.prot087940.

PMID:
27371597
27.

Optimized Affinity Capture of Yeast Protein Complexes.

LaCava J, Fernandez-Martinez J, Hakhverdyan Z, Rout MP.

Cold Spring Harb Protoc. 2016 Jul 1;2016(7). doi: 10.1101/pdb.prot087932.

PMID:
27371596
28.

High-Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes.

Obado SO, Field MC, Chait BT, Rout MP.

Methods Mol Biol. 2016;1411:67-80. doi: 10.1007/978-1-4939-3530-7_3.

PMID:
27147034
29.

Slide-and-exchange mechanism for rapid and selective transport through the nuclear pore complex.

Raveh B, Karp JM, Sparks S, Dutta K, Rout MP, Sali A, Cowburn D.

Proc Natl Acad Sci U S A. 2016 May 3;113(18):E2489-97. doi: 10.1073/pnas.1522663113. Epub 2016 Apr 18.

30.

Revealing Higher Order Protein Structure Using Mass Spectrometry.

Chait BT, Cadene M, Olinares PD, Rout MP, Shi Y.

J Am Soc Mass Spectrom. 2016 Jun;27(6):952-65. doi: 10.1007/s13361-016-1385-1. Epub 2016 Apr 14.

31.

Characterization of L1-Ribonucleoprotein Particles.

Taylor MS, LaCava J, Dai L, Mita P, Burns KH, Rout MP, Boeke JD.

Methods Mol Biol. 2016;1400:311-38. doi: 10.1007/978-1-4939-3372-3_20.

32.

Interactome Mapping Reveals the Evolutionary History of the Nuclear Pore Complex.

Obado SO, Brillantes M, Uryu K, Zhang W, Ketaren NE, Chait BT, Field MC, Rout MP.

PLoS Biol. 2016 Feb 18;14(2):e1002365. doi: 10.1371/journal.pbio.1002365. eCollection 2016 Feb.

33.

Developing genetic tools to exploit Chaetomium thermophilum for biochemical analyses of eukaryotic macromolecular assemblies.

Kellner N, Schwarz J, Sturm M, Fernandez-Martinez J, Griesel S, Zhang W, Chait BT, Rout MP, Kück U, Hurt E.

Sci Rep. 2016 Feb 11;6:20937. doi: 10.1038/srep20937.

34.

A Robust Workflow for Native Mass Spectrometric Analysis of Affinity-Isolated Endogenous Protein Assemblies.

Olinares PD, Dunn AD, Padovan JC, Fernandez-Martinez J, Rout MP, Chait BT.

Anal Chem. 2016 Mar 1;88(5):2799-807. doi: 10.1021/acs.analchem.5b04477. Epub 2016 Feb 18.

35.

Optimizing selection of large animals for antibody production by screening immune response to standard vaccines.

Thompson MK, Fridy PC, Keegan S, Chait BT, Fenyö D, Rout MP.

J Immunol Methods. 2016 Mar;430:56-60. doi: 10.1016/j.jim.2016.01.006. Epub 2016 Jan 9.

36.

Subunit connectivity, assembly determinants and architecture of the yeast exocyst complex.

Heider MR, Gu M, Duffy CM, Mirza AM, Marcotte LL, Walls AC, Farrall N, Hakhverdyan Z, Field MC, Rout MP, Frost A, Munson M.

Nat Struct Mol Biol. 2016 Jan;23(1):59-66. doi: 10.1038/nsmb.3146. Epub 2015 Dec 14.

37.

The interactome challenge.

Aitchison JD, Rout MP.

J Cell Biol. 2015 Nov 23;211(4):729-32. doi: 10.1083/jcb.201510108. Epub 2015 Nov 16.

38.

A strategy for dissecting the architectures of native macromolecular assemblies.

Shi Y, Pellarin R, Fridy PC, Fernandez-Martinez J, Thompson MK, Li Y, Wang QJ, Sali A, Rout MP, Chait BT.

Nat Methods. 2015 Dec;12(12):1135-8. doi: 10.1038/nmeth.3617. Epub 2015 Oct 5.

39.

The molecular mechanism of nuclear transport revealed by atomic-scale measurements.

Hough LE, Dutta K, Sparks S, Temel DB, Kamal A, Tetenbaum-Novatt J, Rout MP, Cowburn D.

Elife. 2015 Sep 15;4. pii: e10027. doi: 10.7554/eLife.10027.

40.

Rapid, optimized interactomic screening.

Hakhverdyan Z, Domanski M, Hough LE, Oroskar AA, Oroskar AR, Keegan S, Dilworth DJ, Molloy KR, Sherman V, Aitchison JD, Fenyö D, Chait BT, Jensen TH, Rout MP, LaCava J.

Nat Methods. 2015 Jun;12(6):553-60. doi: 10.1038/nmeth.3395. Epub 2015 May 4.

41.

SEA you later alli-GATOR--a dynamic regulator of the TORC1 stress response pathway.

Dokudovskaya S, Rout MP.

J Cell Sci. 2015 Jun 15;128(12):2219-28. doi: 10.1242/jcs.168922. Epub 2015 May 1. Review.

42.

Altering nuclear pore complex function impacts longevity and mitochondrial function in S. cerevisiae.

Lord CL, Timney BL, Rout MP, Wente SR.

J Cell Biol. 2015 Mar 16;208(6):729-44. doi: 10.1083/jcb.201412024.

43.

Affinity proteomics to study endogenous protein complexes: pointers, pitfalls, preferences and perspectives.

LaCava J, Molloy KR, Taylor MS, Domanski M, Chait BT, Rout MP.

Biotechniques. 2015 Mar 1;58(3):103-19. doi: 10.2144/000114262. eCollection 2015 Mar.

44.

Engineered high-affinity nanobodies recognizing staphylococcal Protein A and suitable for native isolation of protein complexes.

Fridy PC, Thompson MK, Ketaren NE, Rout MP.

Anal Biochem. 2015 May 15;477:92-4. doi: 10.1016/j.ab.2015.02.013. Epub 2015 Feb 21.

45.

Touching from a distance.

Holden JM, Koreny L, Kelly S, Chait BT, Rout MP, Field MC, Obado SO.

Nucleus. 2014 Jul-Aug;5(4):304-10. doi: 10.4161/nucl.32228.

46.

A robust pipeline for rapid production of versatile nanobody repertoires.

Fridy PC, Li Y, Keegan S, Thompson MK, Nudelman I, Scheid JF, Oeffinger M, Nussenzweig MC, Fenyö D, Chait BT, Rout MP.

Nat Methods. 2014 Dec;11(12):1253-60. doi: 10.1038/nmeth.3170. Epub 2014 Nov 2.

47.

Structural characterization by cross-linking reveals the detailed architecture of a coatomer-related heptameric module from the nuclear pore complex.

Shi Y, Fernandez-Martinez J, Tjioe E, Pellarin R, Kim SJ, Williams R, Schneidman-Duhovny D, Sali A, Rout MP, Chait BT.

Mol Cell Proteomics. 2014 Nov;13(11):2927-43. doi: 10.1074/mcp.M114.041673. Epub 2014 Aug 26.

48.

Integrative structure-function mapping of the nucleoporin Nup133 suggests a conserved mechanism for membrane anchoring of the nuclear pore complex.

Kim SJ, Fernandez-Martinez J, Sampathkumar P, Martel A, Matsui T, Tsuruta H, Weiss TM, Shi Y, Markina-Inarrairaegui A, Bonanno JB, Sauder JM, Burley SK, Chait BT, Almo SC, Rout MP, Sali A.

Mol Cell Proteomics. 2014 Nov;13(11):2911-26. doi: 10.1074/mcp.M114.040915. Epub 2014 Aug 19.

49.

Molecular architecture and function of the SEA complex, a modulator of the TORC1 pathway.

Algret R, Fernandez-Martinez J, Shi Y, Kim SJ, Pellarin R, Cimermancic P, Cochet E, Sali A, Chait BT, Rout MP, Dokudovskaya S.

Mol Cell Proteomics. 2014 Nov;13(11):2855-70. doi: 10.1074/mcp.M114.039388. Epub 2014 Jul 29.

50.

Editorial overview: Cell nucleus: the nucleus: a dynamic organelle.

Rout MP, Karpen GH.

Curr Opin Cell Biol. 2014 Jun;28:iv-vii. doi: 10.1016/j.ceb.2014.05.005. Epub 2014 Jun 4. No abstract available.

PMID:
24908378

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