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A Strategy to Optimize the Generation of Stable Chromobody Cell Lines for Visualization and Quantification of Endogenous Proteins in Living Cells.

Keller BM, Maier J, Weldle M, Segan S, Traenkle B, Rothbauer U.

Antibodies (Basel). 2019 Jan 10;8(1). pii: E10. doi: 10.3390/antib8010010.


Okadaic acid activates Wnt/β-catenin-signaling in human HepaRG cells.

Dietrich J, Sommersdorf C, Gohlke S, Poetz O, Traenkle B, Rothbauer U, Hessel-Pras S, Lampen A, Braeuning A.

Arch Toxicol. 2019 Jul;93(7):1927-1939. doi: 10.1007/s00204-019-02489-4. Epub 2019 May 21.


Chromobodies to Quantify Changes of Endogenous Protein Concentration in Living Cells.

Keller BM, Maier J, Secker KA, Egetemaier SM, Parfyonova Y, Rothbauer U, Traenkle B.

Mol Cell Proteomics. 2018 Dec;17(12):2518-2533. doi: 10.1074/mcp.TIR118.000914. Epub 2018 Sep 18.


Speed up to find the right ones: rapid discovery of functional nanobodies.

Rothbauer U.

Nat Struct Mol Biol. 2018 Mar;25(3):199-201. doi: 10.1038/s41594-018-0038-4. No abstract available.


A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging.

Virant D, Traenkle B, Maier J, Kaiser PD, Bodenhöfer M, Schmees C, Vojnovic I, Pisak-Lukáts B, Endesfelder U, Rothbauer U.

Nat Commun. 2018 Mar 2;9(1):930. doi: 10.1038/s41467-018-03191-2.


Akt1 and Akt3 but not Akt2 through interaction with DNA-PKcs stimulate proliferation and post-irradiation cell survival of K-RAS-mutated cancer cells.

Toulany M, Maier J, Iida M, Rebholz S, Holler M, Grottke A, Jüker M, Wheeler DL, Rothbauer U, Rodemann HP.

Cell Death Discov. 2017 Oct 30;3:17072. doi: 10.1038/cddiscovery.2017.72. eCollection 2017.


Under the Microscope: Single-Domain Antibodies for Live-Cell Imaging and Super-Resolution Microscopy.

Traenkle B, Rothbauer U.

Front Immunol. 2017 Aug 24;8:1030. doi: 10.3389/fimmu.2017.01030. eCollection 2017. Review.


Role of BCL9L in transforming growth factor-β (TGF-β)-induced epithelial-to-mesenchymal-transition (EMT) and metastasis of pancreatic cancer.

Sannino G, Armbruster N, Bodenhöfer M, Haerle U, Behrens D, Buchholz M, Rothbauer U, Sipos B, Schmees C.

Oncotarget. 2016 Nov 8;7(45):73725-73738. doi: 10.18632/oncotarget.12455.


Visualizing Epithelial-Mesenchymal Transition Using the Chromobody Technology.

Maier J, Traenkle B, Rothbauer U.

Cancer Res. 2016 Oct 1;76(19):5592-5596. Epub 2016 Sep 15. Review.


A Nexus Consisting of Beta-Catenin and Stat3 Attenuates BRAF Inhibitor Efficacy and Mediates Acquired Resistance to Vemurafenib.

Sinnberg T, Makino E, Krueger MA, Velic A, Macek B, Rothbauer U, Groll N, Pötz O, Czemmel S, Niessner H, Meier F, Ikenberg K, Garbe C, Schittek B.

EBioMedicine. 2016 Jun;8:132-149. doi: 10.1016/j.ebiom.2016.04.037. Epub 2016 May 1.


Coordinate regulation of Cyp2e1 by β-catenin- and hepatocyte nuclear factor 1α-dependent signaling.

Groll N, Petrikat T, Vetter S, Colnot S, Weiss F, Poetz O, Joos TO, Rothbauer U, Schwarz M, Braeuning A.

Toxicology. 2016 Mar 28;350-352:40-8. doi: 10.1016/j.tox.2016.05.004. Epub 2016 May 3.


A Multiplexed High-Content Screening Approach Using the Chromobody Technology to Identify Cell Cycle Modulators in Living Cells.

Schorpp K, Rothenaigner I, Maier J, Traenkle B, Rothbauer U, Hadian K.

J Biomol Screen. 2016 Oct;21(9):965-77. doi: 10.1177/1087057116641935. Epub 2016 Apr 4.


A New Nanobody-Based Biosensor to Study Endogenous PARP1 In Vitro and in Live Human Cells.

Buchfellner A, Yurlova L, Nüske S, Scholz AM, Bogner J, Ruf B, Zolghadr K, Drexler SE, Drexler GA, Girst S, Greubel C, Reindl J, Siebenwirth C, Romer T, Friedl AA, Rothbauer U.

PLoS One. 2016 Mar 7;11(3):e0151041. doi: 10.1371/journal.pone.0151041. eCollection 2016.


Peptides in headlock--a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy.

Braun MB, Traenkle B, Koch PA, Emele F, Weiss F, Poetz O, Stehle T, Rothbauer U.

Sci Rep. 2016 Jan 21;6:19211. doi: 10.1038/srep19211.


From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK Inhibitors.

Bauer SM, Kubiak JM, Rothbauer U, Laufer S.

Methods Mol Biol. 2016;1360:123-48. doi: 10.1007/978-1-4939-3073-9_10.


Generation of an alpaca-derived nanobody recognizing γ-H2AX.

Rajan M, Mortusewicz O, Rothbauer U, Hastert FD, Schmidthals K, Rapp A, Leonhardt H, Cardoso MC.

FEBS Open Bio. 2015 Sep 21;5:779-88. doi: 10.1016/j.fob.2015.09.005. eCollection 2015.


Real-time analysis of epithelial-mesenchymal transition using fluorescent single-domain antibodies.

Maier J, Traenkle B, Rothbauer U.

Sci Rep. 2015 Aug 21;5:13402. doi: 10.1038/srep13402.


Live imaging of endogenous protein dynamics in zebrafish using chromobodies.

Panza P, Maier J, Schmees C, Rothbauer U, Söllner C.

Development. 2015 May 15;142(10):1879-84. doi: 10.1242/dev.118943.


Towards multiplexed protein-protein interaction analysis using protein tag-specific nanobodies.

Groll N, Emele F, Poetz O, Rothbauer U.

J Proteomics. 2015 Sep 8;127(Pt B):289-99. doi: 10.1016/j.jprot.2015.04.017. Epub 2015 May 1.


Correction to Tetra-substituted pyridinylimidazoles as dual inhibitors of p38α mitogen-activated protein kinase and c-Jun N-terminal kinase 3 for potential treatment of neurodegenerative diseases.

Muth F, Günther M, Bauer SM, Döring E, Fischer S, Maier J, Drückes P, Köppler J, Trappe J, Rothbauer U, Koch P, Laufer SA.

J Med Chem. 2015 Mar 12;58(5):2567. doi: 10.1021/acs.jmedchem.5b00235. Epub 2015 Feb 26. No abstract available.


Monitoring interactions and dynamics of endogenous beta-catenin with intracellular nanobodies in living cells.

Traenkle B, Emele F, Anton R, Poetz O, Haeussler RS, Maier J, Kaiser PD, Scholz AM, Nueske S, Buchfellner A, Romer T, Rothbauer U.

Mol Cell Proteomics. 2015 Mar;14(3):707-23. doi: 10.1074/mcp.M114.044016. Epub 2015 Jan 16.


An intracellular nanotrap redirects proteins and organelles in live bacteria.

Borg S, Popp F, Hofmann J, Leonhardt H, Rothbauer U, Schüler D.

mBio. 2015 Jan 13;6(1). pii: e02117-14. doi: 10.1128/mBio.02117-14.


Tetra-substituted pyridinylimidazoles as dual inhibitors of p38α mitogen-activated protein kinase and c-Jun N-terminal kinase 3 for potential treatment of neurodegenerative diseases.

Muth F, Günther M, Bauer SM, Döring E, Fischer S, Maier J, Drückes P, Köppler J, Trappe J, Rothbauer U, Koch P, Laufer SA.

J Med Chem. 2015 Jan 8;58(1):443-56. doi: 10.1021/jm501557a. Epub 2014 Dec 5. Erratum in: J Med Chem. 2015 Mar 12;58(5):2567.


Recent progress in generating intracellular functional antibody fragments to target and trace cellular components in living cells.

Kaiser PD, Maier J, Traenkle B, Emele F, Rothbauer U.

Biochim Biophys Acta. 2014 Nov;1844(11):1933-1942. doi: 10.1016/j.bbapap.2014.04.019. Epub 2014 May 2. Review.


A versatile assay for RNA-binding proteins in living cells.

Strein C, Alleaume AM, Rothbauer U, Hentze MW, Castello A.

RNA. 2014 May;20(5):721-31. doi: 10.1261/rna.043562.113. Epub 2014 Mar 24.


The fluorescent two-hybrid assay to screen for protein-protein interaction inhibitors in live cells: targeting the interaction of p53 with Mdm2 and Mdm4.

Yurlova L, Derks M, Buchfellner A, Hickson I, Janssen M, Morrison D, Stansfield I, Brown CJ, Ghadessy FJ, Lane DP, Rothbauer U, Zolghadr K, Krausz E.

J Biomol Screen. 2014 Apr;19(4):516-25. doi: 10.1177/1087057113518067. Epub 2014 Jan 29.


c-Jun/c-Fos heterodimers regulate cellular genes via a newly identified class of methylated DNA sequence motifs.

Gustems M, Woellmer A, Rothbauer U, Eck SH, Wieland T, Lutter D, Hammerschmidt W.

Nucleic Acids Res. 2014 Mar;42(5):3059-72. doi: 10.1093/nar/gkt1323. Epub 2013 Dec 25.


Direct and dynamic detection of HIV-1 in living cells.

Helma J, Schmidthals K, Lux V, Nüske S, Scholz AM, Kräusslich HG, Rothbauer U, Leonhardt H.

PLoS One. 2012;7(11):e50026. doi: 10.1371/journal.pone.0050026. Epub 2012 Nov 28.


Cascaded photoinduced drug delivery to cells from multifunctional core-shell mesoporous silica.

Schlossbauer A, Sauer AM, Cauda V, Schmidt A, Engelke H, Rothbauer U, Zolghadr K, Leonhardt H, Bräuchle C, Bein T.

Adv Healthc Mater. 2012 May;1(3):316-20. doi: 10.1002/adhm.201100033. Epub 2012 Apr 2.


Case study on live cell apoptosis-assay using lamin-chromobody cell-lines for high-content analysis.

Zolghadr K, Gregor J, Leonhardt H, Rothbauer U.

Methods Mol Biol. 2012;911:569-75. doi: 10.1007/978-1-61779-968-6_36.


Fluorescent protein specific Nanotraps to study protein-protein interactions and histone-tail peptide binding.

Pichler G, Leonhardt H, Rothbauer U.

Methods Mol Biol. 2012;911:475-83. doi: 10.1007/978-1-61779-968-6_29.


A bacterial-two-hybrid selection system for one-step isolation of intracellularly functional Nanobodies.

Pellis M, Pardon E, Zolghadr K, Rothbauer U, Vincke C, Kinne J, Dierynck I, Hertogs K, Leonhardt H, Messens J, Muyldermans S, Conrath K.

Arch Biochem Biophys. 2012 Oct 15;526(2):114-23. doi: 10.1016/ Epub 2012 May 11.


The fluorescent two-hybrid (F2H) assay for direct analysis of protein-protein interactions in living cells.

Zolghadr K, Rothbauer U, Leonhardt H.

Methods Mol Biol. 2012;812:275-82. doi: 10.1007/978-1-61779-455-1_16.


The nucleoporin Nup358/RanBP2 promotes nuclear import in a cargo- and transport receptor-specific manner.

Wälde S, Thakar K, Hutten S, Spillner C, Nath A, Rothbauer U, Wiemann S, Kehlenbach RH.

Traffic. 2012 Feb;13(2):218-33. doi: 10.1111/j.1600-0854.2011.01302.x. Epub 2011 Nov 21.


Magnetosome expression of functional camelid antibody fragments (nanobodies) in Magnetospirillum gryphiswaldense.

Pollithy A, Romer T, Lang C, Müller FD, Helma J, Leonhardt H, Rothbauer U, Schüler D.

Appl Environ Microbiol. 2011 Sep;77(17):6165-71. doi: 10.1128/AEM.05282-11. Epub 2011 Jul 15.


Engineering antibodies and proteins for molecular in vivo imaging.

Romer T, Leonhardt H, Rothbauer U.

Curr Opin Biotechnol. 2011 Dec;22(6):882-7. doi: 10.1016/j.copbio.2011.06.007. Epub 2011 Jun 25. Review.


Protein-binding assays in biological liquids using microscale thermophoresis.

Wienken CJ, Baaske P, Rothbauer U, Braun D, Duhr S.

Nat Commun. 2010 Oct 19;1:100. doi: 10.1038/ncomms1093.


CpG-methylation regulates a class of Epstein-Barr virus promoters.

Bergbauer M, Kalla M, Schmeinck A, Göbel C, Rothbauer U, Eck S, Benet-Pagès A, Strom TM, Hammerschmidt W.

PLoS Pathog. 2010 Sep 23;6(9):e1001114. doi: 10.1371/journal.ppat.1001114.


Novel antibody derivatives for proteome and high-content analysis.

Schmidthals K, Helma J, Zolghadr K, Rothbauer U, Leonhardt H.

Anal Bioanal Chem. 2010 Aug;397(8):3203-8. doi: 10.1007/s00216-010-3657-0. Epub 2010 Apr 7.


Top-down de Novo protein sequencing of a 13.6 kDa camelid single heavy chain antibody by matrix-assisted laser desorption ionization-time-of-flight/time-of-flight mass spectrometry.

Resemann A, Wunderlich D, Rothbauer U, Warscheid B, Leonhardt H, Fuchser J, Kuhlmann K, Suckau D.

Anal Chem. 2010 Apr 15;82(8):3283-92. doi: 10.1021/ac1000515.


Targeting of the prostacyclin specific IP1 receptor in lungs with molecular conjugates comprising prostaglandin I2 analogues.

Geiger J, Aneja MK, Hasenpusch G, Yüksekdag G, Kummerlöwe G, Luy B, Romer T, Rothbauer U, Rudolph C.

Biomaterials. 2010 Apr;31(10):2903-11. doi: 10.1016/j.biomaterials.2009.12.035. Epub 2009 Dec 31.


Modulation of protein properties in living cells using nanobodies.

Kirchhofer A, Helma J, Schmidthals K, Frauer C, Cui S, Karcher A, Pellis M, Muyldermans S, Casas-Delucchi CS, Cardoso MC, Leonhardt H, Hopfner KP, Rothbauer U.

Nat Struct Mol Biol. 2010 Jan;17(1):133-8. doi: 10.1038/nsmb.1727. Epub 2009 Dec 13.


Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells.

Meilinger D, Fellinger K, Bultmann S, Rothbauer U, Bonapace IM, Klinkert WE, Spada F, Leonhardt H.

EMBO Rep. 2009 Nov;10(11):1259-64. doi: 10.1038/embor.2009.201. Epub 2009 Oct 2.


Protein mislocalization in plant cells using a GFP-binding chromobody.

Schornack S, Fuchs R, Huitema E, Rothbauer U, Lipka V, Kamoun S.

Plant J. 2009 Nov;60(4):744-54. doi: 10.1111/j.1365-313X.2009.03982.x. Epub 2009 Jul 22.


Dimerization of DNA methyltransferase 1 is mediated by its regulatory domain.

Fellinger K, Rothbauer U, Felle M, Längst G, Leonhardt H.

J Cell Biochem. 2009 Mar 1;106(4):521-8. doi: 10.1002/jcb.22071.


Camelid immunoglobulins and nanobody technology.

Muyldermans S, Baral TN, Retamozzo VC, De Baetselier P, De Genst E, Kinne J, Leonhardt H, Magez S, Nguyen VK, Revets H, Rothbauer U, Stijlemans B, Tillib S, Wernery U, Wyns L, Hassanzadeh-Ghassabeh G, Saerens D.

Vet Immunol Immunopathol. 2009 Mar 15;128(1-3):178-83. doi: 10.1016/j.vetimm.2008.10.299. Epub 2008 Oct 17.


Identifying specific protein interaction partners using quantitative mass spectrometry and bead proteomes.

Trinkle-Mulcahy L, Boulon S, Lam YW, Urcia R, Boisvert FM, Vandermoere F, Morrice NA, Swift S, Rothbauer U, Leonhardt H, Lamond A.

J Cell Biol. 2008 Oct 20;183(2):223-39. doi: 10.1083/jcb.200805092.


Generation and characterization of a rat monoclonal antibody specific for PCNA.

Rottach A, Kremmer E, Nowak D, Boisguerin P, Volkmer R, Cardoso MC, Leonhardt H, Rothbauer U.

Hybridoma (Larchmt). 2008 Apr;27(2):91-8.


A fluorescent two-hybrid assay for direct visualization of protein interactions in living cells.

Zolghadr K, Mortusewicz O, Rothbauer U, Kleinhans R, Goehler H, Wanker EE, Cardoso MC, Leonhardt H.

Mol Cell Proteomics. 2008 Nov;7(11):2279-87. doi: 10.1074/mcp.M700548-MCP200. Epub 2008 Jul 12.

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