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Development. 2018 May 23;145(10). pii: dev162107. doi: 10.1242/dev.162107.

Transfection of Capsaspora owczarzaki, a close unicellular relative of animals.

Author information

1
Institut de Biologia Evolutiva (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Catalonia, Spain.
2
Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 5562, Shobara, Hiroshima 727-0023, Japan.
3
Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET) and Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Ocampo y Esmeralda s/n, Rosario S2000FHQ, Argentina.
4
Institut de Biologia Evolutiva (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Catalonia, Spain inaki.ruiz@ibe.upf-csic.es.
5
Departament de Genètica, Microbiologia i Estadística, Universitat de Barcelona, Av. Diagonal, 645, 08028 Barcelona, Catalonia, Spain.
6
ICREA, Passeig Lluís Companys 23, 08010, Barcelona, Catalonia, Spain.

Abstract

How animals emerged from their unicellular ancestor remains a major evolutionary question. New genome data from the closest unicellular relatives of animals have provided important insights into the evolution of animal multicellularity. We know that the unicellular ancestor of animals had an unexpectedly complex genetic repertoire, including many genes that are key to animal development and multicellularity. Thus, assessing the function of these genes among unicellular relatives of animals is key to understanding how they were co-opted at the onset of the Metazoa. However, such analyses have been hampered by the lack of genetic tools. Progress has been made in choanoflagellates and teretosporeans, two of the three lineages closely related to animals, whereas no tools are yet available for functional analysis in the third lineage: the filastereans. Importantly, filastereans have a striking repertoire of genes involved in transcriptional regulation and other developmental processes. Here, we describe a reliable transfection method for the filasterean Capsaspora owczarzaki We also provide a set of constructs for visualising subcellular structures in live cells. These tools convert Capsaspora into a unique experimentally tractable organism to use to investigate the origin and evolution of animal multicellularity.

KEYWORDS:

Capsaspora owczarzaki; Holozoa; Multicellularity; Origin of Metazoa; Transfection

PMID:
29752387
PMCID:
PMC6001378
DOI:
10.1242/dev.162107
[Indexed for MEDLINE]
Free PMC Article

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