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Items: 24


The cell wall regulates dynamics and size of plasma-membrane nanodomains in Arabidopsis.

McKenna JF, Rolfe DJ, Webb SED, Tolmie AF, Botchway SW, Martin-Fernandez ML, Hawes C, Runions J.

Proc Natl Acad Sci U S A. 2019 Jun 25;116(26):12857-12862. doi: 10.1073/pnas.1819077116. Epub 2019 Jun 10.


Solid immersion microscopy images cells under cryogenic conditions with 12 nm resolution.

Wang L, Bateman B, Zanetti-Domingues LC, Moores AN, Astbury S, Spindloe C, Darrow MC, Romano M, Needham SR, Beis K, Rolfe DJ, Clarke DT, Martin-Fernandez ML.

Commun Biol. 2019 Feb 21;2:74. doi: 10.1038/s42003-019-0317-6. eCollection 2019.


The architecture of EGFR's basal complexes reveals autoinhibition mechanisms in dimers and oligomers.

Zanetti-Domingues LC, Korovesis D, Needham SR, Tynan CJ, Sagawa S, Roberts SK, Kuzmanic A, Ortiz-Zapater E, Jain P, Roovers RC, Lajevardipour A, van Bergen En Henegouwen PMP, Santis G, Clayton AHA, Clarke DT, Gervasio FL, Shan Y, Shaw DE, Rolfe DJ, Parker PJ, Martin-Fernandez ML.

Nat Commun. 2018 Oct 18;9(1):4325. doi: 10.1038/s41467-018-06632-0.


EGFR oligomerization organizes kinase-active dimers into competent signalling platforms.

Needham SR, Roberts SK, Arkhipov A, Mysore VP, Tynan CJ, Zanetti-Domingues LC, Kim ET, Losasso V, Korovesis D, Hirsch M, Rolfe DJ, Clarke DT, Winn MD, Lajevardipour A, Clayton AH, Pike LJ, Perani M, Parker PJ, Shan Y, Shaw DE, Martin-Fernandez ML.

Nat Commun. 2016 Oct 31;7:13307. doi: 10.1038/ncomms13307.


Characterisation of the effects of optical aberrations in single molecule techniques.

Coles BC, Webb SE, Schwartz N, Rolfe DJ, Martin-Fernandez M, Lo Schiavo V.

Biomed Opt Express. 2016 Apr 7;7(5):1755-67. doi: 10.1364/BOE.7.001755. eCollection 2016 May 1.


A tale of the epidermal growth factor receptor: The quest for structural resolution on cells.

Tynan CJ, Lo Schiavo V, Zanetti-Domingues L, Needham SR, Roberts SK, Hirsch M, Rolfe DJ, Korovesis D, Clarke DT, Martin-Fernandez ML.

Methods. 2016 Feb 15;95:86-93. doi: 10.1016/j.ymeth.2015.10.009. Epub 2015 Oct 17. Review.


Determining the geometry of oligomers of the human epidermal growth factor family on cells with <10 nm resolution.

Needham SR, Zanetti-Domingues LC, Scherer KM, Hirsch M, Rolfe DJ, Roberts SK, Martin-Fernandez ML, Clarke DT, Tynan CJ.

Biochem Soc Trans. 2015 Jun;43(3):309-14. doi: 10.1042/BST20140318. Review.


Nanometric molecular separation measurements by single molecule photobleaching.

Webb SE, Hirsch M, Needham SR, Coles BC, Scherer KM, Roberts SK, Zanetti-Domingues LC, Tynan CJ, Martin-Fernandez ML, Rolfe DJ.

Methods. 2015 Oct 15;88:76-80. doi: 10.1016/j.ymeth.2015.05.009. Epub 2015 May 14.


Determining the geometry of oligomers of the human epidermal growth factor family on cells with 7 nm resolution.

Zanetti-Domingues LC, Hirsch M, Tynan CJ, Rolfe DJ, Boyadzhiev TV, Scherer KM, Clarke DT, Martin-Fernandez ML, Needham SR.

Prog Biophys Mol Biol. 2015 Sep;118(3):139-52. doi: 10.1016/j.pbiomolbio.2015.04.002. Epub 2015 Apr 18. Review.


Effect of phosphorylation on EGFR dimer stability probed by single-molecule dynamics and FRET/FLIM.

Coban O, Zanetti-Dominguez LC, Matthews DR, Rolfe DJ, Weitsman G, Barber PR, Barbeau J, Devauges V, Kampmeier F, Winn M, Vojnovic B, Parker PJ, Lidke KA, Lidke DS, Ameer-Beg SM, Martin-Fernandez ML, Ng T.

Biophys J. 2015 Mar 10;108(5):1013-26. doi: 10.1016/j.bpj.2015.01.005.


The ErbB4 CYT2 variant protects EGFR from ligand-induced degradation to enhance cancer cell motility.

Kiuchi T, Ortiz-Zapater E, Monypenny J, Matthews DR, Nguyen LK, Barbeau J, Coban O, Lawler K, Burford B, Rolfe DJ, de Rinaldis E, Dafou D, Simpson MA, Woodman N, Pinder S, Gillett CE, Devauges V, Poland SP, Fruhwirth G, Marra P, Boersma YL, Plückthun A, Gullick WJ, Yarden Y, Santis G, Winn M, Kholodenko BN, Martin-Fernandez ML, Parker P, Tutt A, Ameer-Beg SM, Ng T.

Sci Signal. 2014 Aug 19;7(339):ra78. doi: 10.1126/scisignal.2005157.


Structure-function relationships and supramolecular organization of the EGFR (epidermal growth factor receptor) on the cell surface.

Needham SR, Zanetti-Domingues LC, Hirsch M, Rolfe DJ, Tynan CJ, Roberts SK, Martin-Fernandez ML, Clarke DT.

Biochem Soc Trans. 2014 Feb;42(1):114-9. doi: 10.1042/BST20130236. Review.


Hydrophobic fluorescent probes introduce artifacts into single molecule tracking experiments due to non-specific binding.

Zanetti-Domingues LC, Tynan CJ, Rolfe DJ, Clarke DT, Martin-Fernandez M.

PLoS One. 2013 Sep 16;8(9):e74200. doi: 10.1371/journal.pone.0074200. eCollection 2013.


Measuring EGFR separations on cells with ~10 nm resolution via fluorophore localization imaging with photobleaching.

Needham SR, Hirsch M, Rolfe DJ, Clarke DT, Zanetti-Domingues LC, Wareham R, Martin-Fernandez ML.

PLoS One. 2013 May 1;8(5):e62331. doi: 10.1371/journal.pone.0062331. Print 2013.


A stochastic model for electron multiplication charge-coupled devices--from theory to practice.

Hirsch M, Wareham RJ, Martin-Fernandez ML, Hobson MP, Rolfe DJ.

PLoS One. 2013;8(1):e53671. doi: 10.1371/journal.pone.0053671. Epub 2013 Jan 31.


A systematic investigation of differential effects of cell culture substrates on the extent of artifacts in single-molecule tracking.

Zanetti-Domingues LC, Martin-Fernandez ML, Needham SR, Rolfe DJ, Clarke DT.

PLoS One. 2012;7(9):e45655. doi: 10.1371/journal.pone.0045655. Epub 2012 Sep 25.


Cell wall constrains lateral diffusion of plant plasma-membrane proteins.

Martinière A, Lavagi I, Nageswaran G, Rolfe DJ, Maneta-Peyret L, Luu DT, Botchway SW, Webb SE, Mongrand S, Maurel C, Martin-Fernandez ML, Kleine-Vehn J, Friml J, Moreau P, Runions J.

Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12805-10. doi: 10.1073/pnas.1202040109. Epub 2012 Jun 11.


Multicolour single molecule imaging in cells with near infra-red dyes.

Tynan CJ, Clarke DT, Coles BC, Rolfe DJ, Martin-Fernandez ML, Webb SE.

PLoS One. 2012;7(4):e36265. doi: 10.1371/journal.pone.0036265. Epub 2012 Apr 25.


Multicolour single molecule imaging on cells using a supercontinuum source.

Webb SE, Zanetti-Domingues L, Coles BC, Rolfe DJ, Wareham RJ, Martin-Fernandez ML.

Biomed Opt Express. 2012 Mar 1;3(3):400-6. doi: 10.1364/BOE.3.000400. Epub 2012 Feb 2.


Optics clustered to output unique solutions: a multi-laser facility for combined single molecule and ensemble microscopy.

Clarke DT, Botchway SW, Coles BC, Needham SR, Roberts SK, Rolfe DJ, Tynan CJ, Ward AD, Webb SE, Yadav R, Zanetti-Domingues L, Martin-Fernandez ML.

Rev Sci Instrum. 2011 Sep;82(9):093705. doi: 10.1063/1.3635536.


Automated multidimensional single molecule fluorescence microscopy feature detection and tracking.

Rolfe DJ, McLachlan CI, Hirsch M, Needham SR, Tynan CJ, Webb SE, Martin-Fernandez ML, Hobson MP.

Eur Biophys J. 2011 Oct;40(10):1167-86. doi: 10.1007/s00249-011-0747-7. Epub 2011 Sep 18.


Human epidermal growth factor receptor (EGFR) aligned on the plasma membrane adopts key features of Drosophila EGFR asymmetry.

Tynan CJ, Roberts SK, Rolfe DJ, Clarke DT, Loeffler HH, Kästner J, Winn MD, Parker PJ, Martin-Fernandez ML.

Mol Cell Biol. 2011 Jun;31(11):2241-52. doi: 10.1128/MCB.01431-10. Epub 2011 Mar 28.


Simultaneous widefield single molecule orientation and FRET microscopy in cells.

Webb SE, Rolfe DJ, Needham SR, Roberts SK, Clarke DT, McLachlan CI, Hobson MP, Martin-Fernandez ML.

Opt Express. 2008 Dec 8;16(25):20258-65.


Single-molecule imaging and fluorescence lifetime imaging microscopy show different structures for high- and low-affinity epidermal growth factor receptors in A431 cells.

Webb SE, Roberts SK, Needham SR, Tynan CJ, Rolfe DJ, Winn MD, Clarke DT, Barraclough R, Martin-Fernandez ML.

Biophys J. 2008 Feb 1;94(3):803-19. Epub 2007 Sep 21.

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