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Transgenic Res. 2013 Aug;22(4):869-71. doi: 10.1007/s11248-013-9698-3. Epub 2013 Feb 22.

Unexpectedly rapid IS1 transposition into an Arabidopsis chromatin remodeling gene.

Author information

1
Laboratory of Molecular Biology, Plant Sciences Group, Wageningen University and Research Centre, Wageningen, The Netherlands.

Abstract

Common cloning is often associated with instability of certain classes of DNA. Here we report on IS1 transposition as possible source of such instability. During the cloning of Arabidopsis thaliana gene into commercially available vector maintained in widely used Escherichia coli host the insertion of complete IS1 element into the intron of cloned gene was found. The transposition of the IS1 element was remarkably rapid and is likely to be sequence-specific. The use of E. coli strains that lower the copy number of vector or avoiding the presence of the problematic sequence is a solution to the inadvertent transposition of IS1. The transposition of IS1 is rare but it can occur and might confound functional studies of a plant gene.

PMID:
23430589
DOI:
10.1007/s11248-013-9698-3
[Indexed for MEDLINE]

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