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Items: 41

1.

The human secretome.

Uhlén M, Karlsson MJ, Hober A, Svensson AS, Scheffel J, Kotol D, Zhong W, Tebani A, Strandberg L, Edfors F, Sjöstedt E, Mulder J, Mardinoglu A, Berling A, Ekblad S, Dannemeyer M, Kanje S, Rockberg J, Lundqvist M, Malm M, Volk AL, Nilsson P, Månberg A, Dodig-Crnkovic T, Pin E, Zwahlen M, Oksvold P, von Feilitzen K, Häussler RS, Hong MG, Lindskog C, Ponten F, Katona B, Vuu J, Lindström E, Nielsen J, Robinson J, Ayoglu B, Mahdessian D, Sullivan D, Thul P, Danielsson F, Stadler C, Lundberg E, Bergström G, Gummesson A, Voldborg BG, Tegel H, Hober S, Forsström B, Schwenk JM, Fagerberg L, Sivertsson Å.

Sci Signal. 2019 Nov 26;12(609). pii: eaaz0274. doi: 10.1126/scisignal.aaz0274.

PMID:
31772123
2.

Systematic Development of Sandwich Immunoassays for the Plasma Secretome.

Häussler RS, Bendes A, Iglesias M, Sanchez-Rivera L, Dodig-Crnković T, Byström S, Fredolini C, Birgersson E, Dale M, Edfors F, Fagerberg L, Rockberg J, Tegel H, Uhlén M, Qundos U, Schwenk JM.

Proteomics. 2019 Aug;19(15):e1900008. doi: 10.1002/pmic.201900008. Epub 2019 Jul 22.

PMID:
31278833
3.

SAMURAI (Solid-phase Assisted Mutagenesis by Uracil Restriction for Accurate Integration) for antibody affinity maturation and paratope mapping.

Hu FJ, Lundqvist M, Uhlén M, Rockberg J.

Nucleic Acids Res. 2019 Apr 8;47(6):e34. doi: 10.1093/nar/gkz050.

4.

Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion.

Lundqvist M, Thalén N, Volk AL, Hansen HG, von Otter E, Nygren PÅ, Uhlen M, Rockberg J.

Sci Rep. 2019 Jan 22;9(1):310. doi: 10.1038/s41598-018-36559-x.

5.

High Cell Density Perfusion Culture has a Maintained Exoproteome and Metabolome.

Zamani L, Lundqvist M, Zhang Y, Aberg M, Edfors F, Bidkhori G, Lindahl A, Mie A, Mardinoglu A, Field R, Turner R, Rockberg J, Chotteau V.

Biotechnol J. 2018 Oct;13(10):e1800036. doi: 10.1002/biot.201800036. Epub 2018 Jul 13.

PMID:
29957873
6.

Discontinuous Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Folded Domains.

Volk AL, Rockberg J.

Methods Mol Biol. 2018;1785:159-183. doi: 10.1007/978-1-4939-7841-0_11.

PMID:
29714018
7.

Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries.

Volk AL, Hu FJ, Rockberg J.

Methods Mol Biol. 2018;1785:141-157. doi: 10.1007/978-1-4939-7841-0_10.

PMID:
29714017
8.

An Introduction to Epitope Mapping.

Nilvebrant J, Rockberg J.

Methods Mol Biol. 2018;1785:1-10. doi: 10.1007/978-1-4939-7841-0_1.

PMID:
29714008
9.

Combination of phage and Gram-positive bacterial display of human antibody repertoires enables isolation of functional high affinity binders.

Hu FJ, Volk AL, Persson H, Säll A, Borrebaeck C, Uhlen M, Rockberg J.

N Biotechnol. 2018 Oct 25;45:80-88. doi: 10.1016/j.nbt.2017.07.011. Epub 2017 Aug 1.

10.

A subcellular map of the human proteome.

Thul PJ, Åkesson L, Wiking M, Mahdessian D, Geladaki A, Ait Blal H, Alm T, Asplund A, Björk L, Breckels LM, Bäckström A, Danielsson F, Fagerberg L, Fall J, Gatto L, Gnann C, Hober S, Hjelmare M, Johansson F, Lee S, Lindskog C, Mulder J, Mulvey CM, Nilsson P, Oksvold P, Rockberg J, Schutten R, Schwenk JM, Sivertsson Å, Sjöstedt E, Skogs M, Stadler C, Sullivan DP, Tegel H, Winsnes C, Zhang C, Zwahlen M, Mardinoglu A, Pontén F, von Feilitzen K, Lilley KS, Uhlén M, Lundberg E.

Science. 2017 May 26;356(6340). pii: eaal3321. doi: 10.1126/science.aal3321. Epub 2017 May 11.

PMID:
28495876
11.

Machine learning in computational biology to accelerate high-throughput protein expression.

Sastry A, Monk J, Tegel H, Uhlen M, Palsson BO, Rockberg J, Brunk E.

Bioinformatics. 2017 Aug 15;33(16):2487-2495. doi: 10.1093/bioinformatics/btx207.

12.

Risk of mortality and recurrent cardiovascular events in patients with acute coronary syndromes on high intensity statin treatment.

Rockberg J, Jørgensen L, Taylor B, Sobocki P, Johansson G.

Prev Med Rep. 2017 Mar 18;6:203-209. doi: 10.1016/j.pmedr.2017.03.001. eCollection 2017 Jun.

13.

Epidemiology of cutaneous melanoma in Sweden-Stage-specific survival and rate of recurrence.

Rockberg J, Amelio JM, Taylor A, Jörgensen L, Ragnhammar P, Hansson J.

Int J Cancer. 2016 Dec 15;139(12):2722-2729. doi: 10.1002/ijc.30407. Epub 2016 Sep 22.

14.

Stratification of responders towards eculizumab using a structural epitope mapping strategy.

Volk AL, Hu FJ, Berglund MM, Nordling E, Strömberg P, Uhlen M, Rockberg J.

Sci Rep. 2016 Aug 11;6:31365. doi: 10.1038/srep31365.

15.

Population-based study of giant cell tumor of bone in Sweden (1983-2011).

Amelio JM, Rockberg J, Hernandez RK, Sobocki P, Stryker S, Bach BA, Engellau J, Liede A.

Cancer Epidemiol. 2016 Jun;42:82-9. doi: 10.1016/j.canep.2016.03.014. Epub 2016 Apr 6.

PMID:
27060625
16.

Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells.

Hansen HG, Nilsson CN, Lund AM, Kol S, Grav LM, Lundqvist M, Rockberg J, Lee GM, Andersen MR, Kildegaard HF.

Sci Rep. 2015 Dec 11;5:18016. doi: 10.1038/srep18016.

17.

Incidence Trends in the Diagnosis of Giant Cell Tumor of Bone in Sweden Since 1958.

Rockberg J, Bach BA, Amelio J, Hernandez RK, Sobocki P, Engellau J, Bauer HC, Liede A.

J Bone Joint Surg Am. 2015 Nov 4;97(21):1756-66. doi: 10.2106/JBJS.O.00156.

PMID:
26537163
18.

Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production.

Anfelt J, Kaczmarzyk D, Shabestary K, Renberg B, Rockberg J, Nielsen J, Uhlén M, Hudson EP.

Microb Cell Fact. 2015 Oct 16;14:167. doi: 10.1186/s12934-015-0355-9.

19.

Exome sequencing of contralateral breast cancer identifies metastatic disease.

Klevebring D, Lindberg J, Rockberg J, Hilliges C, Hall P, Sandberg M, Czene K.

Breast Cancer Res Treat. 2015 Jun;151(2):319-24. doi: 10.1007/s10549-015-3403-6. Epub 2015 Apr 29.

PMID:
25922084
20.

Dissecting antibodies with regards to linear and conformational epitopes.

Forsström B, Axnäs BB, Rockberg J, Danielsson H, Bohlin A, Uhlen M.

PLoS One. 2015 Mar 27;10(3):e0121673. doi: 10.1371/journal.pone.0121673. eCollection 2015.

21.

Solid-phase cloning for high-throughput assembly of single and multiple DNA parts.

Lundqvist M, Edfors F, Sivertsson Å, Hallström BM, Hudson EP, Tegel H, Holmberg A, Uhlén M, Rockberg J.

Nucleic Acids Res. 2015 Apr 20;43(7):e49. doi: 10.1093/nar/gkv036. Epub 2015 Jan 23.

22.

Proteomics. Tissue-based map of the human proteome.

Uhlén M, Fagerberg L, Hallström BM, Lindskog C, Oksvold P, Mardinoglu A, Sivertsson Å, Kampf C, Sjöstedt E, Asplund A, Olsson I, Edlund K, Lundberg E, Navani S, Szigyarto CA, Odeberg J, Djureinovic D, Takanen JO, Hober S, Alm T, Edqvist PH, Berling H, Tegel H, Mulder J, Rockberg J, Nilsson P, Schwenk JM, Hamsten M, von Feilitzen K, Forsberg M, Persson L, Johansson F, Zwahlen M, von Heijne G, Nielsen J, Pontén F.

Science. 2015 Jan 23;347(6220):1260419. doi: 10.1126/science.1260419.

23.

Combination of novel HER2-targeting antibody 1E11 with trastuzumab shows synergistic antitumor activity in HER2-positive gastric cancer.

Ko BK, Lee SY, Lee YH, Hwang IS, Persson H, Rockberg J, Borrebaeck C, Park D, Kim KT, Uhlen M, Lee JS.

Mol Oncol. 2015 Feb;9(2):398-408. doi: 10.1016/j.molonc.2014.09.007. Epub 2014 Sep 28.

24.

Proteome-wide epitope mapping of antibodies using ultra-dense peptide arrays.

Forsström B, Axnäs BB, Stengele KP, Bühler J, Albert TJ, Richmond TA, Hu FJ, Nilsson P, Hudson EP, Rockberg J, Uhlen M.

Mol Cell Proteomics. 2014 Jun;13(6):1585-97. doi: 10.1074/mcp.M113.033308. Epub 2014 Apr 4.

25.

Epitope mapping of monoclonal and polyclonal antibodies using bacterial cell surface display.

Volk AL, Hu FJ, Rockberg J.

Methods Mol Biol. 2014;1131:485-500. doi: 10.1007/978-1-62703-992-5_29.

PMID:
24515484
26.

Generation of HER2 monoclonal antibodies using epitopes of a rabbit polyclonal antibody.

Hu FJ, Uhlen M, Rockberg J.

N Biotechnol. 2014 Jan 25;31(1):35-43. doi: 10.1016/j.nbt.2013.10.002. Epub 2013 Oct 11.

PMID:
24120493
27.

Parallel immunizations of rabbits using the same antigen yield antibodies with similar, but not identical, epitopes.

Hjelm B, Forsström B, Löfblom J, Rockberg J, Uhlén M.

PLoS One. 2012;7(12):e45817. doi: 10.1371/journal.pone.0045817. Epub 2012 Dec 19.

28.

Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP).

Fagerberg L, Oksvold P, Skogs M, Algenäs C, Lundberg E, Pontén F, Sivertsson A, Odeberg J, Klevebring D, Kampf C, Asplund A, Sjöstedt E, Al-Khalili Szigyarto C, Edqvist PH, Olsson I, Rydberg U, Hudson P, Ottosson Takanen J, Berling H, Björling L, Tegel H, Rockberg J, Nilsson P, Navani S, Jirström K, Mulder J, Schwenk JM, Zwahlen M, Hober S, Forsberg M, von Feilitzen K, Uhlén M.

J Proteome Res. 2013 Jun 7;12(6):2439-48. doi: 10.1021/pr300924j. Epub 2012 Dec 31.

PMID:
23276153
29.

Multiplex epitope mapping using bacterial surface display reveals both linear and conformational epitopes.

Hudson EP, Uhlen M, Rockberg J.

Sci Rep. 2012;2:706. doi: 10.1038/srep00706. Epub 2012 Oct 4.

30.

High-resolution mapping of linear antibody epitopes using ultrahigh-density peptide microarrays.

Buus S, Rockberg J, Forsström B, Nilsson P, Uhlen M, Schafer-Nielsen C.

Mol Cell Proteomics. 2012 Dec;11(12):1790-800. doi: 10.1074/mcp.M112.020800. Epub 2012 Sep 13.

31.

Automated solid-phase subcloning based on beads brought into proximity by magnetic force.

Hudson EP, Nikoshkov A, Uhlen M, Rockberg J.

PLoS One. 2012;7(5):e37429. doi: 10.1371/journal.pone.0037429. Epub 2012 May 18.

32.

Generation of monospecific antibodies based on affinity capture of polyclonal antibodies.

Hjelm B, Forsström B, Igel U, Johannesson H, Stadler C, Lundberg E, Ponten F, Sjöberg A, Rockberg J, Schwenk JM, Nilsson P, Johansson C, Uhlén M.

Protein Sci. 2011 Nov;20(11):1824-35. doi: 10.1002/pro.716. Epub 2011 Oct 12.

33.

Staphylococcal surface display in combinatorial protein engineering and epitope mapping of antibodies.

Kronqvist N, Malm M, Rockberg J, Hjelm B, Uhlén M, Ståhl S, Löfblom J.

Recent Pat Biotechnol. 2010 Nov;4(3):171-82. Review.

PMID:
21171954
34.

Epitope mapping using gram-positive surface display.

Rockberg J, Löfblom J, Hjelm B, Ståhl S, Uhlén M.

Curr Protoc Immunol. 2010 Aug;Chapter 9:Unit9.9. doi: 10.1002/0471142735.im0909s90.

PMID:
20814943
35.

Exploring epitopes of antibodies toward the human tryptophanyl-tRNA synthetase.

Hjelm B, Fernández CD, Löfblom J, Ståhl S, Johannesson H, Rockberg J, Uhlén M.

N Biotechnol. 2010 May 31;27(2):129-37. doi: 10.1016/j.nbt.2009.11.001. Epub 2009 Nov 11.

PMID:
19913117
36.

Prediction of antibody response using recombinant human protein fragments as antigen.

Rockberg J, Uhlén M.

Protein Sci. 2009 Nov;18(11):2346-55. doi: 10.1002/pro.245.

37.

Discovery of epitopes for targeting the human epidermal growth factor receptor 2 (HER2) with antibodies.

Rockberg J, Schwenk JM, Uhlén M.

Mol Oncol. 2009 Jun;3(3):238-47. doi: 10.1016/j.molonc.2009.01.003. Epub 2009 Jan 31.

38.

Epitope mapping of antibodies using bacterial surface display.

Rockberg J, Löfblom J, Hjelm B, Uhlén M, Ståhl S.

Nat Methods. 2008 Dec;5(12):1039-45. doi: 10.1038/nmeth.1272. Epub 2008 Nov 23.

PMID:
19029907
39.

A whole-genome bioinformatics approach to selection of antigens for systematic antibody generation.

Berglund L, Björling E, Jonasson K, Rockberg J, Fagerberg L, Al-Khalili Szigyarto C, Sivertsson A, Uhlén M.

Proteomics. 2008 Jul;8(14):2832-9. doi: 10.1002/pmic.200800203.

PMID:
18655051
40.

A human protein atlas for normal and cancer tissues based on antibody proteomics.

Uhlén M, Björling E, Agaton C, Szigyarto CA, Amini B, Andersen E, Andersson AC, Angelidou P, Asplund A, Asplund C, Berglund L, Bergström K, Brumer H, Cerjan D, Ekström M, Elobeid A, Eriksson C, Fagerberg L, Falk R, Fall J, Forsberg M, Björklund MG, Gumbel K, Halimi A, Hallin I, Hamsten C, Hansson M, Hedhammar M, Hercules G, Kampf C, Larsson K, Lindskog M, Lodewyckx W, Lund J, Lundeberg J, Magnusson K, Malm E, Nilsson P, Odling J, Oksvold P, Olsson I, Oster E, Ottosson J, Paavilainen L, Persson A, Rimini R, Rockberg J, Runeson M, Sivertsson A, Sköllermo A, Steen J, Stenvall M, Sterky F, Strömberg S, Sundberg M, Tegel H, Tourle S, Wahlund E, Waldén A, Wan J, Wernérus H, Westberg J, Wester K, Wrethagen U, Xu LL, Hober S, Pontén F.

Mol Cell Proteomics. 2005 Dec;4(12):1920-32. Epub 2005 Aug 27.

41.

Selection of protein epitopes for antibody production.

Lindskog M, Rockberg J, Uhlén M, Sterky F.

Biotechniques. 2005 May;38(5):723-7.

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