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Nat Methods. 2019 Dec;16(12):1269-1273. doi: 10.1038/s41592-019-0623-4. Epub 2019 Nov 11.

Non-reversible tissue fixation retains extracellular vesicles for in situ imaging.

Author information

1
Department of Ophthalmology and Margaret M. Dyson Vision Research Institute, Weill Cornell Medicine, New York, NY, USA.
2
Department of Pathology, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands.
3
Freeman School of Business, Tulane University, New Orleans, LA, USA.
4
The Rockefeller University, Proteomics Resource Center, New York, NY, USA.
5
Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA.
6
Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA.
7
Department of Ophthalmology and Margaret M. Dyson Vision Research Institute, Weill Cornell Medicine, New York, NY, USA. jpena65@gmail.com.

Abstract

Extracellular vesicles (EVs) are secreted nanosized particles with many biological functions and pathological associations. The inability to image EVs in fixed tissues has been a major limitation to understanding their role in healthy and diseased tissue microenvironments. Here, we show that crosslinking mammalian tissues with formaldehyde results in significant EV loss, which can be prevented by additional fixation with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) for visualization of EVs in a range of normal and cancer tissues.

PMID:
31712780
DOI:
10.1038/s41592-019-0623-4

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