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Cell Host Microbe. 2019 Nov 4. pii: S1931-3128(19)30528-1. doi: 10.1016/j.chom.2019.10.004. [Epub ahead of print]

An RNA-Binding Protein Secreted by a Bacterial Pathogen Modulates RIG-I Signaling.

Author information

1
Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France; U604 Inserm, Paris, France; USC2020 INRA, Paris, France. Electronic address: alessandro.pagliuso@inra.fr.
2
Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France; U604 Inserm, Paris, France; USC2020 INRA, Paris, France.
3
Unité de régulation épigénétique, Institut Pasteur, UMR3738 CNRS, Paris, France.
4
Laboratoire Pathogenèse des Bactéries Anaérobies, Institut Pasteur, Paris, Université de Paris, Paris, France.
5
Université Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale (IBS), Bacterial Pathogenesis Group, Grenoble, France.
6
Hub de bioinformatique et biostatistique - Centre de Bioinformatique, Biostatistique et Biologie Intégrative, Unité mixte de Service et Recherche 3756 Institut Pasteur - Centre National de la Recherche Scientifique, Paris 75015, France.
7
Unité de Génomique Virale et Vaccination, Institut Pasteur, Paris 75015, France; CNRS UMR-3569, Paris, France.
8
Department I of Internal Medicine, University Hospital Cologne, Cologne, Germany; Cologne Excellence Cluster on Cellular Stress Response in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany; Department of Translational Epigenetics and Tumor Genetics, University Hospital Cologne, Cologne, Germany.
9
Université Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale (IBS), Bacterial Pathogenesis Group, Grenoble, France; Brazilian Biosciences National Laboratory (LNBio), CNPEM, Campinas, SP, Brazil.
10
Équipe Infection et Devenir de l'ARN, Institut de biologie de l'Ecole normale supérieure (IBENS), Ecole normale supérieure, CNRS, Inserm, PSL Université Paris, Paris 75005, France; INRA, IBENS, 75005 Paris, France.
11
Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France; U604 Inserm, Paris, France; USC2020 INRA, Paris, France. Electronic address: pcossart@pasteur.fr.

Abstract

RNA-binding proteins (RBPs) perform key cellular activities by controlling the function of bound RNAs. The widely held assumption that RBPs are strictly intracellular has been challenged by the discovery of secreted RBPs. However, extracellular RBPs have been described in eukaryotes, while secreted bacterial RBPs have not been reported. Here, we show that the bacterial pathogen Listeria monocytogenes secretes a small RBP that we named Zea. We show that Zea binds a subset of L. monocytogenes RNAs, causing their accumulation in the extracellular medium. Furthermore, during L. monocytogenes infection, Zea binds RIG-I, the non-self-RNA innate immunity sensor, potentiating interferon-β production. Mouse infection studies reveal that Zea affects L. monocytogenes virulence. Together, our results unveil that bacterial RNAs can be present extracellularly in association with RBPs, acting as "social RNAs" to trigger a host response during infection.

KEYWORDS:

bacteriophage A118; extracellular RNA; type I IFN

PMID:
31761719
DOI:
10.1016/j.chom.2019.10.004
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