Format

Send to

Choose Destination
Sci Immunol. 2018 Nov 16;3(29). pii: eaau5265. doi: 10.1126/sciimmunol.aau5265.

Single-cell transcriptional analysis reveals ILC-like cells in zebrafish.

Author information

1
Macrophages et Développement de l'Immunité, Institut Pasteur, Paris, France. as889@cam.ac.uk jean-pierre.levraud@pasteur.fr pedro.hernandez-cerda@pasteur.fr.
2
Centre National de la Recherche Scientifique, UMR3738, Paris, France.
3
Department of Haematology, University of Cambridge, Cambridge, UK.
4
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK.
5
Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, Cambridge, UK.
6
Macrophages et Développement de l'Immunité, Institut Pasteur, Paris, France.
7
Marine Scotland Science, Marine Laboratory, Aberdeen, UK.
8
Institut National de la Recherche Agronomique, Virologie et Immunologie Moléculaire, Jouy-en-Josas, France.
9
Department of Haematology, University of Cambridge, Cambridge, UK. as889@cam.ac.uk jean-pierre.levraud@pasteur.fr pedro.hernandez-cerda@pasteur.fr.

Abstract

Innate lymphoid cells (ILCs) are important mediators of the immune response and homeostasis in barrier tissues of mammals. However, the existence and function of ILCs in other vertebrates are poorly understood. Here, we use single-cell RNA sequencing to generate a comprehensive atlas of zebrafish lymphocytes during tissue homeostasis and after immune challenge. We profiled 14,080 individual cells from the gut of wild-type zebrafish, as well as of rag1-deficient zebrafish that lack T and B cells, and discovered populations of ILC-like cells. We uncovered a rorc-positive subset of ILCs that could express cytokines associated with type 1, 2, and 3 responses upon immune challenge. Specifically, these ILC-like cells expressed il22 and tnfa after exposure to inactivated bacteria or il13 after exposure to helminth extract. Cytokine-producing ILC-like cells express a specific repertoire of novel immune-type receptors, likely involved in recognition of environmental cues. We identified additional novel markers of zebrafish ILCs and generated a cloud repository for their in-depth exploration.

PMID:
30446505
PMCID:
PMC6258902
[Available on 2019-05-16]
DOI:
10.1126/sciimmunol.aau5265

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center