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Appl Environ Microbiol. 2014 Jun;80(11):3384-93. doi: 10.1128/AEM.00299-14. Epub 2014 Mar 21.

Deletion mutant library for investigation of functional outputs of cyclic diguanylate metabolism in Pseudomonas aeruginosa PA14.

Author information

1
Geisel School of Medicine at Dartmouth, Department of Microbiology and Immunology, Hanover, New Hampshire, USA.

Abstract

We constructed a library of in-frame deletion mutants targeting each gene in Pseudomonas aeruginosa PA14 predicted to participate in cyclic di-GMP (c-di-GMP) metabolism (biosynthesis or degradation) to provide a toolkit to assist investigators studying c-di-GMP-mediated regulation by this microbe. We present phenotypic assessments of each mutant, including biofilm formation, exopolysaccharide (EPS) production, swimming motility, swarming motility, and twitch motility, as a means to initially characterize these mutants and to demonstrate the potential utility of this library.

PMID:
24657857
PMCID:
PMC4018857
DOI:
10.1128/AEM.00299-14
[Indexed for MEDLINE]
Free PMC Article

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