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Dis Model Mech. 2018 Nov 8. pii: dmm.035097. doi: 10.1242/dmm.035097. [Epub ahead of print]

Establishment of a murine culture system for modeling the temporal progression of cranial and trunk neural crest cell differentiation.

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Department of Biological Sciences, University of Wisconsin - Milwaukee, Milwaukee WI, USA.
Joseph J. Zilber School of Public Health, University of Wisconsin - Milwaukee, Milwaukee WI, USA.
STEMCELL Technologies, Vancouver, BC, Canada.
Department of Biological Sciences, University of Wisconsin - Milwaukee, Milwaukee WI, USA


The neural crest (NC) is a transient population of embryonic progenitors that are implicated in a diverse range of congenital birth defects and pediatric syndromes. The broad spectrum of NC-related disorders can be attributed to the wide variety of differentiated cell types arising from the NC. In vitro models of NC development provide a powerful platform for testing the relative contributions of intrinsic and extrinsic factors mediating NC differentiation under normal and pathogenic conditions. Although differentiation is a dynamic process that unfolds over time, currently, there is no well-defined chronology that characterizes the in vitro progression of NC differentiation towards specific cell fates. In this study, we have optimized culture conditions for expansion of primary murine NC cells that give rise to both ectodermal and mesoectodermal derivatives, even after multiple passages. Significantly, we have delineated highly reproducible timelines that include distinct intermediate stages for lineage-specific NC differentiation in vitro In addition, isolating both cranial and trunk NC cells from the same embryos enabled us to make direct comparisons between the two cell populations over the course of differentiation. Our results define characteristic changes in cell morphology and behavior that track the temporal progression of NC cells as they differentiate along the neuronal, glial, and chondrogenic lineages in vitro These benchmarks constitute a chronological baseline for assessing how genetic or environmental disruptions may facilitate or impede NC differentiation. Introducing a temporal dimension substantially increases the power of this platform for screening drugs or chemicals for developmental toxicity or therapeutic potential.


Chondrogenic differentiation; Differentiation timeline; Glial differentiation; In vitro expansion; Neuronal differentiation; Sox9

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