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G3 (Bethesda). 2018 Jan 4;8(1):343-351. doi: 10.1534/g3.117.300416.

Caenorhabditis elegans DBL-1/BMP Regulates Lipid Accumulation via Interaction with Insulin Signaling.

Author information

1
Ph.D. Program in Biology, The Graduate Center, City University of New York (CUNY), New York, New York 10016.
2
Biology Department, Queens College, CUNY, Flushing, New York 11367.
3
Department of Neuroscience, Albert Einstein College of Medicine, New York, New York 10461.
4
Ph.D. Program in Biology, The Graduate Center, City University of New York (CUNY), New York, New York 10016 cathy.savagedunn@qc.cuny.edu.

Abstract

Metabolic homeostasis is coordinately controlled by diverse inputs. Understanding these regulatory networks is vital to combating metabolic disorders. The nematode Caenorhabditis elegans has emerged as a powerful, genetically tractable model system for the discovery of lipid regulatory mechanisms. Here we introduce DBL-1, the C. elegans homolog of bone morphogenetic protein 2/4 (BMP2/4), as a significant regulator of lipid homeostasis. We used neutral lipid staining and a lipid droplet marker to demonstrate that both increases and decreases in DBL-1/BMP signaling result in reduced lipid stores and lipid droplet count. We find that lipid droplet size, however, correlates positively with the level of DBL-1/BMP signaling. Regulation of lipid accumulation in the intestine occurs through non-cell-autonomous signaling, since expression of SMA-3, a Smad signal transducer, in the epidermis (hypodermis) is sufficient to rescue the loss of lipid accumulation. Finally, genetic evidence indicates that DBL-1/BMP functions upstream of Insulin/IGF-1 Signaling in lipid metabolism. We conclude that BMP signaling regulates lipid metabolism in C. elegans through interorgan signaling to the Insulin pathway, shedding light on a less well-studied regulatory mechanism for metabolic homeostasis.

KEYWORDS:

BMP; Caenorhabditis elegans; homeostasis; insulin; lipid

PMID:
29162682
PMCID:
PMC5765361
DOI:
10.1534/g3.117.300416
[Indexed for MEDLINE]
Free PMC Article

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