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Microbiologyopen. 2019 Jul 4:e893. doi: 10.1002/mbo3.893. [Epub ahead of print]

The influence of temperature and pH on bacterial community composition of microbial mats in hot springs from Costa Rica.

Author information

1
Centro de Investigación en Biología Celular y Molecular (CIBCM), Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, San José, Costa Rica.
2
Escuela Centroamericana de Geología, Universidad de Costa Rica, San José, Costa Rica.
3
Departamento de Microbiología y Genética, Pontificia Universidad Católica de Chile, Santiago, Chile.
4
Departamento de Biología de Sistemas, Centro Nacional de Biotecnología, CSIC, Madrid, Spain.

Abstract

We used the 16S rRNA gene pyrosequencing approach to investigate the microbial diversity and community composition in several Costa Rican hot springs alongside the latitudinal axis of the country, with a range of temperatures (37-63°C), pH (6-7.5) and other geochemical conditions. A principal component analyses of the physicochemical parameters showed the samples were separated into three geochemically distinct habitats associated with the location (North, Central, and South). Cyanobacteria and Chloroflexi comprised 93% of the classified community, the former being the most abundant phylum in all samples except for Rocas Calientes 1, (63°C, pH 6), where Chloroflexi and Deinococcus-Thermus represented 84% of the OTUs. Chloroflexi were more abundant as temperature increased. Proteobacteria, Bacteriodetes and Deinococcus-Thermus comprised 5% of the OTUs represented. Other Phyla were present in very small percentages (<1%). A LINKTREE analysis showed that the community structure of the mats was shaped primarily by pH, separating samples with pH > 6.6 from samples with pH < 6.4. Thus, both pH and temperature were relevant for community composition even within the moderate ranges of variables studied. These results provide a basis for an understanding of the physicochemical influences in moderately thermophilic microbial mats.

KEYWORDS:

Chloroflexi ; cyanobacteria; hot springs; phototrophic mats; pyrosequencing

PMID:
31271524
DOI:
10.1002/mbo3.893
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