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Nacre-inspired hemicelluloses paper with fire retardant and gas barrier properties by self-assembly with bentonite nanosheets.

Huang C, Fang G, Tao Y, Meng X, Lin Y, Bhagia S, Wu X, Yong Q, Ragauskas AJ.

Carbohydr Polym. 2019 Dec 1;225:115219. doi: 10.1016/j.carbpol.2019.115219. Epub 2019 Aug 20.


Cross-Linked Nanocellulosic Materials and Their Applications.

Liang L, Bhagia S, Li M, Huang C, Ragauskas AJ.

ChemSusChem. 2019 Aug 26. doi: 10.1002/cssc.201901676. [Epub ahead of print] Review.


A Comprehensive Characterization of Pyrolysis Oil from Softwood Barks.

Ben H, Wu F, Wu Z, Han G, Jiang W, Ragauskas AJ.

Polymers (Basel). 2019 Aug 23;11(9). pii: E1387. doi: 10.3390/polym11091387.


Determination of hydroxyl groups in biorefinery resources via quantitative 31P NMR spectroscopy.

Meng X, Crestini C, Ben H, Hao N, Pu Y, Ragauskas AJ, Argyropoulos DS.

Nat Protoc. 2019 Sep;14(9):2627-2647. doi: 10.1038/s41596-019-0191-1. Epub 2019 Aug 7.


Mechanism-Guided Design of Highly Efficient Protein Secretion and Lipid Conversion for Biomanufacturing and Biorefining.

Xie S, Sun S, Lin F, Li M, Pu Y, Cheng Y, Xu B, Liu Z, da Costa Sousa L, Dale BE, Ragauskas AJ, Dai SY, Yuan JS.

Adv Sci (Weinh). 2019 May 1;6(13):1801980. doi: 10.1002/advs.201801980. eCollection 2019 Jul 3.


Dynamic Self-Assembly of Polyelectrolyte Composite Nanomaterial Film.

Hou Q, Wang X, Ragauskas AJ.

Polymers (Basel). 2019 Jul 30;11(8). pii: E1258. doi: 10.3390/polym11081258.


Bio-inspired nanocomposite by layer-by-layer coating of chitosan/hyaluronic acid multilayers on a hard nanocellulose-hydroxyapatite matrix.

Huang C, Fang G, Zhao Y, Bhagia S, Meng X, Yong Q, Ragauskas AJ.

Carbohydr Polym. 2019 Oct 15;222:115036. doi: 10.1016/j.carbpol.2019.115036. Epub 2019 Jun 28.


A Multifunctional Cosolvent Pair Reveals Molecular Principles of Biomass Deconstruction.

Patri AS, Mostofian B, Pu Y, Ciaffone N, Soliman M, Smith MD, Kumar R, Cheng X, Wyman CE, Tetard L, Ragauskas AJ, Smith JC, Petridis L, Cai CM.

J Am Chem Soc. 2019 Aug 14;141(32):12545-12557. doi: 10.1021/jacs.8b10242. Epub 2019 Jul 31.


Investigating the correlation of biomass recalcitrance with pyrolysis oil using poplar as the feedstock.

Lu K, Hao N, Meng X, Luo Z, Tuskan GA, Ragauskas AJ.

Bioresour Technol. 2019 Oct;289:121589. doi: 10.1016/j.biortech.2019.121589. Epub 2019 Jun 1.


One-pot transformation of lignocellulosic biomass into crude bio-oil with metal chlorides via hydrothermal and supercritical ethanol processing.

Hao N, Alper K, Tekin K, Karagoz S, Ragauskas AJ.

Bioresour Technol. 2019 Sep;288:121500. doi: 10.1016/j.biortech.2019.121500. Epub 2019 May 17.


Rhodococcus and Yarrowia-Based Lipid Production Using Lignin-Containing Industrial Residues.

Le RK, Mahan KM, Ragauskas AJ.

Methods Mol Biol. 2019;1995:103-120. doi: 10.1007/978-1-4939-9484-7_5.


Preparation and Characterization of Microcellulose and Nanocellulose Fibers from Artemisia Vulgaris Bast.

Nie K, Song Y, Liu S, Han G, Ben H, Ragauskas AJ, Jiang W.

Polymers (Basel). 2019 May 19;11(5). pii: E907. doi: 10.3390/polym11050907.


Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana.

Xie H, Engle NL, Venketachalam S, Yoo CG, Barros J, Lecoultre M, Howard N, Li G, Sun L, Srivastava AC, Pattathil S, Pu Y, Hahn MG, Ragauskas AJ, Nelson RS, Dixon RA, Tschaplinski TJ, Blancaflor EB, Tang Y.

Biotechnol Biofuels. 2019 May 3;12:108. doi: 10.1186/s13068-019-1446-3. eCollection 2019.


Non-Solvent Fractionation of Lignin Enhances Carbon Fiber Performance.

Li Q, Li M, Lin HS, Hu C, Truong P, Zhang T, Sue HJ, Pu Y, Ragauskas AJ, Yuan JS.

ChemSusChem. 2019 Jul 19;12(14):3249-3256. doi: 10.1002/cssc.201901052. Epub 2019 Jun 18.


A finalized determinant for complete lignocellulose enzymatic saccharification potential to maximize bioethanol production in bioenergy Miscanthus.

Alam A, Zhang R, Liu P, Huang J, Wang Y, Hu Z, Madadi M, Sun D, Hu R, Ragauskas AJ, Tu Y, Peng L.

Biotechnol Biofuels. 2019 Apr 27;12:99. doi: 10.1186/s13068-019-1437-4. eCollection 2019.


Population-level approaches reveal novel aspects of lignin biosynthesis, content, composition and structure.

Tuskan GA, Muchero W, Tschaplinski TJ, Ragauskas AJ.

Curr Opin Biotechnol. 2019 Apr;56:250-257. doi: 10.1016/j.copbio.2019.02.017. Epub 2019 Mar 27. Review.


Cross-linked poly(methyl vinyl ether-co-maleic acid)/poly(ethylene glycol)/nanocellulosics foams via directional freezing.

Liang L, Huang C, Hao N, Ragauskas AJ.

Carbohydr Polym. 2019 Jun 1;213:346-351. doi: 10.1016/j.carbpol.2019.02.073. Epub 2019 Feb 22.


Production of deuterated biomass by cultivation of Lemna minor (duckweed) in D2O.

Evans BR, Foston M, O'Neill HM, Reeves D, Rempe C, McGrath K, Ragauskas AJ, Davison BH.

Planta. 2019 May;249(5):1465-1475. doi: 10.1007/s00425-019-03097-3. Epub 2019 Jan 29.


Downregulation of pectin biosynthesis gene GAUT4 leads to reduced ferulate and lignin-carbohydrate cross-linking in switchgrass.

Li M, Yoo CG, Pu Y, Biswal AK, Tolbert AK, Mohnen D, Ragauskas AJ.

Commun Biol. 2019 Jan 17;2:22. doi: 10.1038/s42003-018-0265-6. eCollection 2019.


A New Calmodulin-Binding Protein Expresses in the Context of Secondary Cell Wall Biosynthesis and Impacts Biomass Properties in Populus.

Badmi R, Payyavula RS, Bali G, Guo HB, Jawdy SS, Gunter LE, Yang X, Winkeler KA, Collins C, Rottmann WH, Yee K, Rodriguez M Jr, Sykes RW, Decker SR, Davis MF, Ragauskas AJ, Tuskan GA, Kalluri UC.

Front Plant Sci. 2018 Dec 5;9:1669. doi: 10.3389/fpls.2018.01669. eCollection 2018.


Hemicellulose-Cellulose Composites Reveal Differences in Cellulose Organization after Dilute Acid Pretreatment.

Shah R, Huang S, Pingali SV, Sawada D, Pu Y, Rodriguez M Jr, Ragauskas AJ, Kim SH, Evans BR, Davison BH, O'Neill H.

Biomacromolecules. 2019 Feb 11;20(2):893-903. doi: 10.1021/acs.biomac.8b01511. Epub 2019 Jan 10.


Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440.

Xu Z, Li X, Hao N, Pan C, de la Torre L, Ahamed A, Miller JH, Ragauskas AJ, Yuan J, Yang B.

Bioresour Technol. 2019 Feb;273:538-544. doi: 10.1016/j.biortech.2018.11.046. Epub 2018 Nov 12.


Fractionation and characterization of lignin streams from unique high-lignin content endocarp feedstocks.

Li W, Amos K, Li M, Pu Y, Debolt S, Ragauskas AJ, Shi J.

Biotechnol Biofuels. 2018 Nov 8;11:304. doi: 10.1186/s13068-018-1305-7. eCollection 2018.


Characterization of fractional cuts of co-solvent enhanced lignocellulosic fractionation lignin isolated by sequential precipitation.

Meng X, Parikh A, Seemala B, Kumar R, Pu Y, Wyman CE, Cai CM, Ragauskas AJ.

Bioresour Technol. 2019 Jan;272:202-208. doi: 10.1016/j.biortech.2018.09.130. Epub 2018 Sep 27.


Natural deep eutectic solvents for lignocellulosic biomass pretreatment: Recent developments, challenges and novel opportunities.

Satlewal A, Agrawal R, Bhagia S, Sangoro J, Ragauskas AJ.

Biotechnol Adv. 2018 Dec;36(8):2032-2050. doi: 10.1016/j.biotechadv.2018.08.009. Epub 2018 Sep 5. Review.


Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass.

Bhagia S, Meng X, Evans BR, Dunlap JR, Bali G, Chen J, Reeves KS, Ho HC, Davison BH, Pu Y, Ragauskas AJ.

Sci Rep. 2018 Sep 5;8(1):13226. doi: 10.1038/s41598-018-31269-w.


Ethanol: A Promising Green Solvent for the Deconstruction of Lignocellulose.

Tekin K, Hao N, Karagoz S, Ragauskas AJ.

ChemSusChem. 2018 Oct 24;11(20):3559-3575. doi: 10.1002/cssc.201801291. Epub 2018 Sep 24. Review.


Hemicellulose characterization of deuterated switchgrass.

Bhagia S, Pu Y, Evans BR, Davison BH, Ragauskas AJ.

Bioresour Technol. 2018 Dec;269:567-570. doi: 10.1016/j.biortech.2018.08.034. Epub 2018 Aug 11.


Functional Analysis of Cellulose Synthase CesA4 and CesA6 Genes in Switchgrass (Panicum virgatum) by Overexpression and RNAi-Mediated Gene Silencing.

Mazarei M, Baxter HL, Li M, Biswal AK, Kim K, Meng X, Pu Y, Wuddineh WA, Zhang JY, Turner GB, Sykes RW, Davis MF, Udvardi MK, Wang ZY, Mohnen D, Ragauskas AJ, Labbé N, Stewart CN Jr.

Front Plant Sci. 2018 Aug 3;9:1114. doi: 10.3389/fpls.2018.01114. eCollection 2018.


Production of single cell protein from agro-waste using Rhodococcus opacus.

Mahan KM, Le RK, Wells T Jr, Anderson S, Yuan JS, Stoklosa RJ, Bhalla A, Hodge DB, Ragauskas AJ.

J Ind Microbiol Biotechnol. 2018 Sep;45(9):795-801. doi: 10.1007/s10295-018-2043-3. Epub 2018 Jun 18.


Understanding the influences of different pretreatments on recalcitrance of Populus natural variants.

Yao L, Yang H, Yoo CG, Pu Y, Meng X, Muchero W, Tuskan GA, Tschaplinski T, Ragauskas AJ.

Bioresour Technol. 2018 Oct;265:75-81. doi: 10.1016/j.biortech.2018.05.057. Epub 2018 May 18.


A structured understanding of cellobiohydrolase I binding to poplar lignin fractions after dilute acid pretreatment.

Yao L, Yoo CG, Meng X, Li M, Pu Y, Ragauskas AJ, Yang H.

Biotechnol Biofuels. 2018 Apr 4;11:96. doi: 10.1186/s13068-018-1087-y. eCollection 2018.


Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis.

Biswal AK, Atmodjo MA, Li M, Baxter HL, Yoo CG, Pu Y, Lee YC, Mazarei M, Black IM, Zhang JY, Ramanna H, Bray AL, King ZR, LaFayette PR, Pattathil S, Donohoe BS, Mohanty SS, Ryno D, Yee K, Thompson OA, Rodriguez M Jr, Dumitrache A, Natzke J, Winkeler K, Collins C, Yang X, Tan L, Sykes RW, Gjersing EL, Ziebell A, Turner GB, Decker SR, Hahn MG, Davison BH, Udvardi MK, Mielenz JR, Davis MF, Nelson RS, Parrott WA, Ragauskas AJ, Neal Stewart C Jr, Mohnen D.

Nat Biotechnol. 2018 Mar;36(3):249-257. doi: 10.1038/nbt.4067. Epub 2018 Feb 12.


Laccase-mediated functionalization of chitosan with 4-hexyloxyphenol enhances antioxidant and hydrophobic properties of copolymer.

Liu N, Ni S, Ragauskas AJ, Meng X, Hao N, Fu Y.

J Biotechnol. 2018 Mar 10;269:8-15. doi: 10.1016/j.jbiotec.2018.01.015. Epub 2018 Feb 4.


Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus.

Yang Y, Yoo CG, Winkeler KA, Collins CM, Hinchee MAW, Jawdy SS, Gunter LE, Engle NL, Pu Y, Yang X, Tschaplinski TJ, Ragauskas AJ, Tuskan GA, Chen JG.

Biotechnol Biofuels. 2017 Dec 27;10:311. doi: 10.1186/s13068-017-0998-3. eCollection 2017.


Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release.

Park JJ, Yoo CG, Flanagan A, Pu Y, Debnath S, Ge Y, Ragauskas AJ, Wang ZY.

Biotechnol Biofuels. 2017 Nov 30;10:284. doi: 10.1186/s13068-017-0972-0. eCollection 2017.


Dynamic changes in transcriptome and cell wall composition underlying brassinosteroid-mediated lignification of switchgrass suspension cells.

Rao X, Shen H, Pattathil S, Hahn MG, Gelineo-Albersheim I, Mohnen D, Pu Y, Ragauskas AJ, Chen X, Chen F, Dixon RA.

Biotechnol Biofuels. 2017 Nov 30;10:266. doi: 10.1186/s13068-017-0954-2. eCollection 2017.


Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes.

Thomas VA, Donohoe BS, Li M, Pu Y, Ragauskas AJ, Kumar R, Nguyen TY, Cai CM, Wyman CE.

Biotechnol Biofuels. 2017 Nov 30;10:252. doi: 10.1186/s13068-017-0937-3. eCollection 2017.


The effect of liquid hot water pretreatment on the chemical-structural alteration and the reduced recalcitrance in poplar.

Li M, Cao S, Meng X, Studer M, Wyman CE, Ragauskas AJ, Pu Y.

Biotechnol Biofuels. 2017 Nov 30;10:237. doi: 10.1186/s13068-017-0926-6. eCollection 2017.


Co-production of bio-ethanol, xylonic acid and slow-release nitrogen fertilizer from low-cost straw pulping solid residue.

Huang C, Ragauskas AJ, Wu X, Huang Y, Zhou X, He J, Huang C, Lai C, Li X, Yong Q.

Bioresour Technol. 2018 Feb;250:365-373. doi: 10.1016/j.biortech.2017.11.060. Epub 2017 Nov 22.


Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus.

Le RK, Das P, Mahan KM, Anderson SA, Wells T Jr, Yuan JS, Ragauskas AJ.

AMB Express. 2017 Sep 29;7(1):185. doi: 10.1186/s13568-017-0484-0.


Adsorption of cellobiohydrolases I onto lignin fractions from dilute acid pretreated Broussonetia papyrifera.

Yao L, Yang H, Yoo CG, Meng X, Li M, Pu Y, Ragauskas AJ, Sykes RW.

Bioresour Technol. 2017 Nov;244(Pt 1):957-962. doi: 10.1016/j.biortech.2017.08.024. Epub 2017 Aug 8.


Characterization of products from hydrothermal carbonization of pine.

Wu Q, Yu S, Hao N, Wells T Jr, Meng X, Li M, Pu Y, Liu S, Ragauskas AJ.

Bioresour Technol. 2017 Nov;244(Pt 1):78-83. doi: 10.1016/j.biortech.2017.07.138. Epub 2017 Jul 27.


Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii.

Zurawski JV, Khatibi PA, Akinosho HO, Straub CT, Compton SH, Conway JM, Lee LL, Ragauskas AJ, Davison BH, Adams MWW, Kelly RM.

Appl Environ Microbiol. 2017 Aug 17;83(17). pii: e00969-17. doi: 10.1128/AEM.00969-17. Print 2017 Sep 1.


Laccase-mediated synthesis of lignin-core hyperbranched copolymers.

Cannatelli MD, Ragauskas AJ.

Appl Microbiol Biotechnol. 2017 Aug;101(16):6343-6353. doi: 10.1007/s00253-017-8325-2. Epub 2017 Jun 6.


Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus.

Yang Y, Yoo CG, Guo HB, Rottmann W, Winkeler KA, Collins CM, Gunter LE, Jawdy SS, Yang X, Guo H, Pu Y, Ragauskas AJ, Tuskan GA, Chen JG.

Biotechnol Biofuels. 2017 Mar 23;10:74. doi: 10.1186/s13068-017-0760-x. eCollection 2017.


OsCESA9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice.

Li F, Xie G, Huang J, Zhang R, Li Y, Zhang M, Wang Y, Li A, Li X, Xia T, Qu C, Hu F, Ragauskas AJ, Peng L.

Plant Biotechnol J. 2017 Sep;15(9):1093-1104. doi: 10.1111/pbi.12700. Epub 2017 Mar 15.


Study of traits and recalcitrance reduction of field-grown COMT down-regulated switchgrass.

Li M, Pu Y, Yoo CG, Gjersing E, Decker SR, Doeppke C, Shollenberger T, Tschaplinski TJ, Engle NL, Sykes RW, Davis MF, Baxter HL, Mazarei M, Fu C, Dixon RA, Wang ZY, Neal Stewart C Jr, Ragauskas AJ.

Biotechnol Biofuels. 2017 Jan 3;10:12. doi: 10.1186/s13068-016-0695-7. eCollection 2017.


Current Understanding of the Correlation of Lignin Structure with Biomass Recalcitrance.

Li M, Pu Y, Ragauskas AJ.

Front Chem. 2016 Nov 18;4:45. eCollection 2016. Review.


An In-Depth Understanding of Biomass Recalcitrance Using Natural Poplar Variants as the Feedstock.

Meng X, Pu Y, Yoo CG, Li M, Bali G, Park DY, Gjersing E, Davis MF, Muchero W, Tuskan GA, Tschaplinski TJ, Ragauskas AJ.

ChemSusChem. 2017 Jan 10;10(1):139-150. doi: 10.1002/cssc.201601303. Epub 2016 Dec 12.


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