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Talanta. 1969 Feb;16(2):282-3.

Microdetermination of iron in plant tissue with 4,7-diphenyl-1,10-phenanthroline.

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1
Pacific Southwest Forest and Range Experiment Station Forest Service U.S. Department of Agriculture P.O. Box 245, 1960 Addison St. Berkeley, California 94701, U.S.A.

Abstract

A method is described for the wet digestion and colorimetric determination of small amounts of iron in plant tissues. The wet digestion uses a mixture of sulphuric and nitric acids instead of perchloric acid; a volumetric flask is used for weighing, digestion, and dilution. The iron complex with 4,7-diphenyl-1,10-phenanthroline is extracted into hexanol.

PMID:
18960497

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