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J Clin Microbiol. 1999 Mar;37(3):804-6.

Application of dried blood spot specimens for serologic subtyping of human immunodeficiency virus type 1 in Thailand.

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Army Institute of Pathology, Bangkok, Thailand.


Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as either HIV-1 subtype B or E. Serotype results with DBS and plasma were identical for 254 of 257 specimens. A simple DBS collection method provides a convenient alternative for conducting HIV-1 serotype surveillance while retaining sensitivity and specificity.

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