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Items: 1 to 50 of 111

1.

Expression and purification of recombinant G protein-coupled receptors: A review.

Wiseman DN, Otchere A, Patel JH, Uddin R, Pollock NL, Routledge SJ, Rothnie AJ, Slack C, Poyner DR, Bill RM, Goddard AD.

Protein Expr Purif. 2019 Oct 31:105524. doi: 10.1016/j.pep.2019.105524. [Epub ahead of print]

2.

hCALCRL mutation causes autosomal recessive nonimmune hydrops fetalis with lymphatic dysplasia.

Mackie DI, Al Mutairi F, Davis RB, Kechele DO, Nielsen NR, Snyder JC, Caron MG, Kliman HJ, Berg JS, Simms J, Poyner DR, Caron KM.

J Exp Med. 2018 Sep 3;215(9):2339-2353. doi: 10.1084/jem.20180528. Epub 2018 Aug 16.

3.

Photoaffinity Cross-Linking and Unnatural Amino Acid Mutagenesis Reveal Insights into Calcitonin Gene-Related Peptide Binding to the Calcitonin Receptor-like Receptor/Receptor Activity-Modifying Protein 1 (CLR/RAMP1) Complex.

Simms J, Uddin R, Sakmar TP, Gingell JJ, Garelja ML, Hay DL, Brimble MA, Harris PW, Reynolds CA, Poyner DR.

Biochemistry. 2018 Aug 14;57(32):4915-4922. doi: 10.1021/acs.biochem.8b00502. Epub 2018 Jul 25.

4.

Microbial expression systems for membrane proteins.

Dilworth MV, Piel MS, Bettaney KE, Ma P, Luo J, Sharples D, Poyner DR, Gross SR, Moncoq K, Henderson PJF, Miroux B, Bill RM.

Methods. 2018 Sep 1;147:3-39. doi: 10.1016/j.ymeth.2018.04.009. Epub 2018 Apr 12. Review.

5.

Update on the pharmacology of calcitonin/CGRP family of peptides: IUPHAR Review 25.

Hay DL, Garelja ML, Poyner DR, Walker CS.

Br J Pharmacol. 2018 Jan;175(1):3-17. doi: 10.1111/bph.14075. Epub 2017 Nov 28. Review.

6.

Receptor activity-modifying protein dependent and independent activation mechanisms in the coupling of calcitonin gene-related peptide and adrenomedullin receptors to Gs.

Woolley MJ, Reynolds CA, Simms J, Walker CS, Mobarec JC, Garelja ML, Conner AC, Poyner DR, Hay DL.

Biochem Pharmacol. 2017 Oct 15;142:96-110. doi: 10.1016/j.bcp.2017.07.005. Epub 2017 Jul 11.

7.

Relative Antagonism of Mutants of the CGRP Receptor Extracellular Loop 2 Domain (ECL2) Using a Truncated Competitive Antagonist (CGRP8-37): Evidence for the Dual Involvement of ECL2 in the Two-Domain Binding Model.

Woolley MJ, Simms J, Uddin S, Poyner DR, Conner AC.

Biochemistry. 2017 Aug 1;56(30):3877-3880. doi: 10.1021/acs.biochem.7b00077. Epub 2017 Jul 18.

PMID:
28691801
8.

Understanding the molecular functions of the second extracellular loop (ECL2) of the calcitonin gene-related peptide (CGRP) receptor using a comprehensive mutagenesis approach.

Woolley MJ, Simms J, Mobarec JC, Reynolds CA, Poyner DR, Conner AC.

Mol Cell Endocrinol. 2017 Oct 15;454:39-49. doi: 10.1016/j.mce.2017.05.034. Epub 2017 May 30.

PMID:
28572046
9.

The effects of RAMPs upon cell signalling.

Routledge SJ, Ladds G, Poyner DR.

Mol Cell Endocrinol. 2017 Jul 5;449:12-20. doi: 10.1016/j.mce.2017.03.033. Epub 2017 Apr 5. Review.

PMID:
28390954
10.

Receptor activity-modifying protein-directed G protein signaling specificity for the calcitonin gene-related peptide family of receptors.

Weston C, Winfield I, Harris M, Hodgson R, Shah A, Dowell SJ, Mobarec JC, Woodcock DA, Reynolds CA, Poyner DR, Watkins HA, Ladds G.

J Biol Chem. 2016 Dec 2;291(49):25763. No abstract available.

11.

Receptor Activity-modifying Protein-directed G Protein Signaling Specificity for the Calcitonin Gene-related Peptide Family of Receptors.

Weston C, Winfield I, Harris M, Hodgson R, Shah A, Dowell SJ, Mobarec JC, Woodlock DA, Reynolds CA, Poyner DR, Watkins HA, Ladds G.

J Biol Chem. 2016 Oct 14;291(42):21925-21944. Epub 2016 Aug 26. Erratum in: J Biol Chem. 2016 Dec 2;291(49):25763.

12.

Erratum: An allosteric role for receptor activity-modifying proteins in defining GPCR pharmacology.

Gingell JJ, Simms J, Barwell J, Poyner DR, Watkins HA, Pioszak AA, Sexton PM, Hay DL.

Cell Discov. 2016 Jun 21;2:16020. doi: 10.1038/celldisc.2016.20. eCollection 2016.

13.

An allosteric role for receptor activity-modifying proteins in defining GPCR pharmacology.

J Gingell J, Simms J, Barwell J, Poyner DR, Watkins HA, Pioszak AA, Sexton PM, Hay DL.

Cell Discov. 2016 May 17;2:16012. doi: 10.1038/celldisc.2016.12. eCollection 2016. Erratum in: Cell Discov. 2016;2:16020.

14.

GPCR-styrene maleic acid lipid particles (GPCR-SMALPs): their nature and potential.

Wheatley M, Charlton J, Jamshad M, Routledge SJ, Bailey S, La-Borde PJ, Azam MT, Logan RT, Bill RM, Dafforn TR, Poyner DR.

Biochem Soc Trans. 2016 Apr 15;44(2):619-23. doi: 10.1042/BST20150284. Review.

PMID:
27068979
15.

Receptor activity-modifying proteins; multifunctional G protein-coupled receptor accessory proteins.

Hay DL, Walker CS, Gingell JJ, Ladds G, Reynolds CA, Poyner DR.

Biochem Soc Trans. 2016 Apr 15;44(2):568-73. doi: 10.1042/BST20150237. Review.

PMID:
27068971
16.

Receptor Activity-modifying Proteins 2 and 3 Generate Adrenomedullin Receptor Subtypes with Distinct Molecular Properties.

Watkins HA, Chakravarthy M, Abhayawardana RS, Gingell JJ, Garelja M, Pardamwar M, McElhinney JM, Lathbridge A, Constantine A, Harris PW, Yuen TY, Brimble MA, Barwell J, Poyner DR, Woolley MJ, Conner AC, Pioszak AA, Reynolds CA, Hay DL.

J Biol Chem. 2016 May 27;291(22):11657-75. doi: 10.1074/jbc.M115.688218. Epub 2016 Mar 24.

17.

Structural Basis for Receptor Activity-Modifying Protein-Dependent Selective Peptide Recognition by a G Protein-Coupled Receptor.

Booe JM, Walker CS, Barwell J, Kuteyi G, Simms J, Jamaluddin MA, Warner ML, Bill RM, Harris PW, Brimble MA, Poyner DR, Hay DL, Pioszak AA.

Mol Cell. 2015 Jun 18;58(6):1040-52. doi: 10.1016/j.molcel.2015.04.018. Epub 2015 May 14.

18.

G-protein coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent.

Jamshad M, Charlton J, Lin YP, Routledge SJ, Bawa Z, Knowles TJ, Overduin M, Dekker N, Dafforn TR, Bill RM, Poyner DR, Wheatley M.

Biosci Rep. 2015 Apr 16;35(2). pii: e00188. doi: 10.1042/BSR20140171.

19.

Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks.

Bawa Z, Routledge SJ, Jamshad M, Clare M, Sarkar D, Dickerson I, Ganzlin M, Poyner DR, Bill RM.

Microb Cell Fact. 2014 Sep 4;13(1):127. doi: 10.1186/s12934-014-0127-y.

20.

The Concise Guide to PHARMACOLOGY 2013/14: overview.

Alexander SP, Benson HE, Faccenda E, Pawson AJ, Sharman JL, McGrath JC, Catterall WA, Spedding M, Peters JA, Harmar AJ; CGTP Collaborators, Abul-Hasn N, Anderson CM, Anderson CM, Araiksinen MS, Arita M, Arthofer E, Barker EL, Barratt C, Barnes NM, Bathgate R, Beart PM, Belelli D, Bennett AJ, Birdsall NJ, Boison D, Bonner TI, Brailsford L, Bröer S, Brown P, Calo G, Carter WG, Catterall WA, Chan SL, Chao MV, Chiang N, Christopoulos A, Chun JJ, Cidlowski J, Clapham DE, Cockcroft S, Connor MA, Cox HM, Cuthbert A, Dautzenberg FM, Davenport AP, Dawson PA, Dent G, Dijksterhuis JP, Dollery CT, Dolphin AC, Donowitz M, Dubocovich ML, Eiden L, Eidne K, Evans BA, Fabbro D, Fahlke C, Farndale R, Fitzgerald GA, Fong TM, Fowler CJ, Fry JR, Funk CD, Futerman AH, Ganapathy V, Gaisnier B, Gershengorn MA, Goldin A, Goldman ID, Gundlach AL, Hagenbuch B, Hales TG, Hammond JR, Hamon M, Hancox JC, Hauger RL, Hay DL, Hobbs AJ, Hollenberg MD, Holliday ND, Hoyer D, Hynes NA, Inui KI, Ishii S, Jacobson KA, Jarvis GE, Jarvis MF, Jensen R, Jones CE, Jones RL, Kaibuchi K, Kanai Y, Kennedy C, Kerr ID, Khan AA, Klienz MJ, Kukkonen JP, Lapoint JY, Leurs R, Lingueglia E, Lippiat J, Lolait SJ, Lummis SC, Lynch JW, MacEwan D, Maguire JJ, Marshall IL, May JM, McArdle CA, McGrath JC, Michel MC, Millar NS, Miller LJ, Mitolo V, Monk PN, Moore PK, Moorhouse AJ, Mouillac B, Murphy PM, Neubig RR, Neumaier J, Niesler B, Obaidat A, Offermanns S, Ohlstein E, Panaro MA, Parsons S, Pwrtwee RG, Petersen J, Pin JP, Poyner DR, Prigent S, Prossnitz ER, Pyne NJ, Pyne S, Quigley JG, Ramachandran R, Richelson EL, Roberts RE, Roskoski R, Ross RA, Roth M, Rudnick G, Ryan RM, Said SI, Schild L, Sanger GJ, Scholich K, Schousboe A, Schulte G, Schulz S, Serhan CN, Sexton PM, Sibley DR, Siegel JM, Singh G, Sitsapesan R, Smart TG, Smith DM, Soga T, Stahl A, Stewart G, Stoddart LA, Summers RJ, Thorens B, Thwaites DT, Toll L, Traynor JR, Usdin TB, Vandenberg RJ, Villalon C, Vore M, Waldman SA, Ward DT, Willars GB, Wonnacott SJ, Wright E, Ye RD, Yonezawa A, Zimmermann M.

Br J Pharmacol. 2013 Dec;170(8):1449-58. doi: 10.1111/bph.12444.

21.

Receptor activity-modifying protein-dependent effects of mutations in the calcitonin receptor-like receptor: implications for adrenomedullin and calcitonin gene-related peptide pharmacology.

Watkins HA, Walker CS, Ly KN, Bailey RJ, Barwell J, Poyner DR, Hay DL.

Br J Pharmacol. 2014 Feb;171(3):772-88. doi: 10.1111/bph.12508.

22.

Structure-activity relationships of the N-terminus of calcitonin gene-related peptide: key roles of alanine-5 and threonine-6 in receptor activation.

Hay DL, Harris PW, Kowalczyk R, Brimble MA, Rathbone DL, Barwell J, Conner AC, Poyner DR.

Br J Pharmacol. 2014 Jan;171(2):415-26. doi: 10.1111/bph.12464.

23.

The role of ECL2 in CGRP receptor activation: a combined modelling and experimental approach.

Woolley MJ, Watkins HA, Taddese B, Karakullukcu ZG, Barwell J, Smith KJ, Hay DL, Poyner DR, Reynolds CA, Conner AC.

J R Soc Interface. 2013 Sep 18;10(88):20130589. doi: 10.1098/rsif.2013.0589. Print 2013 Nov 6.

24.

The activation of the CGRP receptor.

Barwell J, Wheatley M, Conner AC, Taddese B, Vohra S, Reynolds CA, Poyner DR.

Biochem Soc Trans. 2013 Feb 1;41(1):180-4. doi: 10.1042/BST20120251. Review.

PMID:
23356280
25.

G-protein-coupled receptors: from structural insights to functional mechanisms.

Chini B, Parenti M, Poyner DR, Wheatley M.

Biochem Soc Trans. 2013 Feb 1;41(1):135-6. doi: 10.1042/BST20120344. Review.

PMID:
23356272
26.

Similarity between class A and class B G-protein-coupled receptors exemplified through calcitonin gene-related peptide receptor modelling and mutagenesis studies.

Vohra S, Taddese B, Conner AC, Poyner DR, Hay DL, Barwell J, Reeves PJ, Upton GJ, Reynolds CA.

J R Soc Interface. 2012 Dec 12;10(79):20120846. doi: 10.1098/rsif.2012.0846. Print 2013 Feb.

27.

Structure-activity relationships for α-calcitonin gene-related peptide.

Watkins HA, Rathbone DL, Barwell J, Hay DL, Poyner DR.

Br J Pharmacol. 2013 Dec;170(7):1308-22. doi: 10.1111/bph.12072. Review.

28.

Receptor activity modifying proteins (RAMPs) interact with the VPAC2 receptor and CRF1 receptors and modulate their function.

Wootten D, Lindmark H, Kadmiel M, Willcockson H, Caron KM, Barwell J, Drmota T, Poyner DR.

Br J Pharmacol. 2013 Feb;168(4):822-34. doi: 10.1111/j.1476-5381.2012.02202.x.

29.

Secretin family (Class B) G protein-coupled receptors - from molecular to clinical perspectives.

Poyner DR, Hay DL.

Br J Pharmacol. 2012 May;166(1):1-3. doi: 10.1111/j.1476-5381.2011.01810.x.

30.

The role of the extracellular loops of the CGRP receptor, a family B GPCR.

Barwell J, Woolley MJ, Wheatley M, Conner AC, Poyner DR.

Biochem Soc Trans. 2012 Apr;40(2):433-7. doi: 10.1042/BST20110726. Review.

PMID:
22435826
31.

RAMPs as drug targets.

Sexton PM, Poyner DR, Simms J, Christopoulos A, Hay DL.

Adv Exp Med Biol. 2012;744:61-74. doi: 10.1007/978-1-4614-2364-5_6. Review.

PMID:
22434108
32.

RAMPs and CGRP receptors.

Barwell J, Wootten D, Simms J, Hay DL, Poyner DR.

Adv Exp Med Biol. 2012;744:13-24. doi: 10.1007/978-1-4614-2364-5_2. Review.

PMID:
22434104
33.

Lifting the lid on GPCRs: the role of extracellular loops.

Wheatley M, Wootten D, Conner MT, Simms J, Kendrick R, Logan RT, Poyner DR, Barwell J.

Br J Pharmacol. 2012 Mar;165(6):1688-1703. doi: 10.1111/j.1476-5381.2011.01629.x. Review.

34.

Extracellular loops 1 and 3 and their associated transmembrane regions of the calcitonin receptor-like receptor are needed for CGRP receptor function.

Barwell J, Conner A, Poyner DR.

Biochim Biophys Acta. 2011 Oct;1813(10):1906-16. doi: 10.1016/j.bbamcr.2011.06.005. Epub 2011 Jun 16.

35.

The pharmacology of adrenomedullin 2/intermedin.

Hong Y, Hay DL, Quirion R, Poyner DR.

Br J Pharmacol. 2012 May;166(1):110-20. doi: 10.1111/j.1476-5381.2011.01530.x. Review.

36.

Calcitonin and calcitonin receptor-like receptors: common themes with family B GPCRs?

Barwell J, Gingell JJ, Watkins HA, Archbold JK, Poyner DR, Hay DL.

Br J Pharmacol. 2012 May;166(1):51-65. doi: 10.1111/j.1476-5381.2011.01525.x. Review.

37.

The use of site-directed mutagenesis to study GPCRs.

Conner AC, Barwell J, Poyner DR, Wheatley M.

Methods Mol Biol. 2011;746:85-98. doi: 10.1007/978-1-61779-126-0_5.

PMID:
21607853
38.

Surfactant-free purification of membrane proteins with intact native membrane environment.

Jamshad M, Lin YP, Knowles TJ, Parslow RA, Harris C, Wheatley M, Poyner DR, Bill RM, Thomas OR, Overduin M, Dafforn TR.

Biochem Soc Trans. 2011 Jun;39(3):813-8. doi: 10.1042/BST0390813. Review.

PMID:
21599653
39.

Structure-function analysis of amino acid 74 of human RAMP1 and RAMP3 and its role in peptide interactions with adrenomedullin and calcitonin gene-related peptide receptors.

Qi T, Ly K, Poyner DR, Christopoulos G, Sexton PM, Hay DL.

Peptides. 2011 May;32(5):1060-7. doi: 10.1016/j.peptides.2011.03.004. Epub 2011 Mar 22.

PMID:
21402116
40.

Adrenomedullin and calcitonin gene-related peptide receptors in endocrine-related cancers: opportunities and challenges.

Hay DL, Walker CS, Poyner DR.

Endocr Relat Cancer. 2010 Dec 13;18(1):C1-14. doi: 10.1677/ERC-10-0244. Print 2011 Feb. Review.

PMID:
21051558
41.

Regulation of signal transduction by calcitonin gene-related peptide receptors.

Walker CS, Conner AC, Poyner DR, Hay DL.

Trends Pharmacol Sci. 2010 Oct;31(10):476-83. doi: 10.1016/j.tips.2010.06.006. Epub 2010 Jul 13. Review.

PMID:
20633935
42.

Evidence that interaction between conserved residues in transmembrane helices 2, 3, and 7 are crucial for human VPAC1 receptor activation.

Chugunov AO, Simms J, Poyner DR, Dehouck Y, Rooman M, Gilis D, Langer I.

Mol Pharmacol. 2010 Sep;78(3):394-401. doi: 10.1124/mol.110.063578. Epub 2010 Jun 23.

PMID:
20573782
43.

Functional characterization of two human receptor activity-modifying protein 3 variants.

Bailey RJ, Bradley JW, Poyner DR, Rathbone DL, Hay DL.

Peptides. 2010 Apr;31(4):579-84. doi: 10.1016/j.peptides.2009.12.016. Epub 2009 Dec 23.

PMID:
20034525
44.

Structure-function analysis of RAMP1-RAMP3 chimeras.

Qi T, Simms J, Bailey RJ, Wheatley M, Rathbone DL, Hay DL, Poyner DR.

Biochemistry. 2010 Jan 26;49(3):522-31. doi: 10.1021/bi9019093.

PMID:
20017504
45.

CGRP receptor antagonists: design and screening.

Poyner DR, Hay DL, Conner AC.

Expert Opin Drug Discov. 2009 Dec;4(12):1253-65. doi: 10.1517/17460440903413496.

PMID:
23480465
46.

Non-peptidic antagonists of the CGRP receptor, BIBN4096BS and MK-0974, interact with the calcitonin receptor-like receptor via methionine-42 and RAMP1 via tryptophan-74.

Miller PS, Barwell J, Poyner DR, Wigglesworth MJ, Garland SL, Donnelly D.

Biochem Biophys Res Commun. 2010 Jan 1;391(1):437-42. doi: 10.1016/j.bbrc.2009.11.076. Epub 2009 Nov 13.

47.

Mapping interaction sites within the N-terminus of the calcitonin gene-related peptide receptor; the role of residues 23-60 of the calcitonin receptor-like receptor.

Barwell J, Miller PS, Donnelly D, Poyner DR.

Peptides. 2010 Jan;31(1):170-6. doi: 10.1016/j.peptides.2009.10.021. Epub 2009 Nov 11.

48.

Calcitonin gene-related peptide, adrenomedullin and flushing.

Hay DL, Poyner DR.

Maturitas. 2009 Oct 20;64(2):104-8. doi: 10.1016/j.maturitas.2009.08.011. Epub 2009 Sep 16. Review.

PMID:
19762180
49.

Novel peptide antagonists of adrenomedullin and calcitonin gene-related peptide receptors: identification, pharmacological characterization, and interactions with position 74 in receptor activity-modifying protein 1/3.

Robinson SD, Aitken JF, Bailey RJ, Poyner DR, Hay DL.

J Pharmacol Exp Ther. 2009 Nov;331(2):513-21. doi: 10.1124/jpet.109.156448. Epub 2009 Jul 30. Erratum in: J Pharmacol Exp Ther. 2011 Oct;339(1):321.

PMID:
19644039
50.

Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield.

Bonander N, Darby RA, Grgic L, Bora N, Wen J, Brogna S, Poyner DR, O'Neill MA, Bill RM.

Microb Cell Fact. 2009 Jan 29;8:10. doi: 10.1186/1475-2859-8-10.

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