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J Exp Biol. 2019 Mar 18;222(Pt 6). pii: jeb194480. doi: 10.1242/jeb.194480.

Environmental temperature alters the overall digestive energetics and differentially affects dietary protein and lipid use in a lizard.

Author information

1
Centre for Invasion Biology, Department of Botany and Zoology, Stellenbosch University, Stellenbosch 7600, South Africa melissaplasman@hotmail.com.
2
Sable Systems International, Las Vegas, NV 89032, USA.
3
Centre for Invasion Biology, Department of Botany and Zoology, Stellenbosch University, Stellenbosch 7600, South Africa.
4
Instituto de Biología, Departamento de Zoología, Universidad Nacional Autónoma de México, Ciudad de México, 04510, Mexico.
5
Centre for Invasion Biology, Department of Conservation Ecology and Entomology, Stellenbosch University, Stellenbosch 7600, South Africa.

Abstract

Processing food (e.g. ingestion, digestion, assimilation) requires energy referred to as specific dynamic action (SDA) and is at least partially fuelled by oxidation of the nutrients (e.g. proteins and lipids) within the recently ingested meal. In ectotherms, environmental temperature can affect the magnitude and/or duration of the SDA, but is likely to also alter the mixture of nutrients that are oxidized to cover these costs. Here, we examined metabolic rate, gut passage time, assimilation efficiency and fuel use in the lizard Agama atra digesting cricket meals at three ecologically relevant temperatures (20, 25 and 32°C). Crickets were isotopically enriched with 13C-leucine or 13C-palmitic-acid tracers to distinguish between protein and lipid oxidation, respectively. Our results show that higher temperatures increased the magnitude of the SDA peak (by 318% between 32 and 20°C) and gut passage rate (63%), and decreased the duration of the SDA response (by 20% for males and 48% for females). Peak rate of dietary protein oxidation occurred sooner than peak lipid oxidation at all temperatures (70, 60 and 31 h earlier for 20, 25 and 32°C, respectively). Assimilation efficiency of proteins, but not lipids, was positively related to temperature. Interestingly, the SDA response exhibited a notable circadian rhythm. These results show that temperature has a pronounced effect on digestive energetics in A. atra, and that this effect differs between nutrient classes. Variation in environmental temperatures may thus alter the energy budget and nutrient reserves of these animals.

KEYWORDS:

Gut passage time; Metabolic rate; Nutrient assimilation; Nutrient oxidation; Specific dynamic action; Stable isotopes

PMID:
30886030
DOI:
10.1242/jeb.194480

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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