Format

Send to

Choose Destination
Cell Rep. 2017 Oct 24;21(4):1102-1115. doi: 10.1016/j.celrep.2017.10.013.

Tracking the Same Neurons across Multiple Days in Ca2+ Imaging Data.

Author information

1
Department of Neurobiology, Weizmann Institute of Science, Rehovot 76100, Israel.
2
Department of Neurobiology, Weizmann Institute of Science, Rehovot 76100, Israel. Electronic address: yaniv.ziv@weizmann.ac.il.

Abstract

Ca2+ imaging techniques permit time-lapse recordings of neuronal activity from large populations over weeks. However, without identifying the same neurons across imaging sessions (cell registration), longitudinal analysis of the neural code is restricted to population-level statistics. Accurate cell registration becomes challenging with increased numbers of cells, sessions, and inter-session intervals. Current cell registration practices, whether manual or automatic, do not quantitatively evaluate registration accuracy, possibly leading to data misinterpretation. We developed a probabilistic method that automatically registers cells across multiple sessions and estimates the registration confidence for each registered cell. Using large-scale Ca2+ imaging data recorded over weeks from the hippocampus and cortex of freely behaving mice, we show that our method performs more accurate registration than previously used routines, yielding estimated error rates <5%, and that the registration is scalable for many sessions. Thus, our method allows reliable longitudinal analysis of the same neurons over long time periods.

KEYWORDS:

GCaMP6; calcium imaging; cell registration; fluorescence imaging; hippocampus; image alignment; microendoscopy; miniature microscopes; place cells; two-photon microscopy

PMID:
29069591
PMCID:
PMC5670033
DOI:
10.1016/j.celrep.2017.10.013
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center