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Items: 1 to 50 of 148


Protease-associated import systems are widespread in Gram-negative bacteria.

Grinter R, Leung PM, Wijeyewickrema LC, Littler D, Beckham S, Pike RN, Walker D, Greening C, Lithgow T.

PLoS Genet. 2019 Oct 15;15(10):e1008435. doi: 10.1371/journal.pgen.1008435. eCollection 2019 Oct.


Determination of the crystal structure and substrate specificity of ananain.

Yongqing T, Wilmann PG, Pan J, West ML, Brown TJ, Mynott T, Pike RN, Wijeyewickrema LC.

Biochimie. 2019 Nov;166:194-202. doi: 10.1016/j.biochi.2019.07.011. Epub 2019 Jul 12.


Twenty years of bioinformatics research for protease-specific substrate and cleavage site prediction: a comprehensive revisit and benchmarking of existing methods.

Li F, Wang Y, Li C, Marquez-Lago TT, Leier A, Rawlings ND, Haffari G, Revote J, Akutsu T, Chou KC, Purcell AW, Pike RN, Webb GI, Ian Smith A, Lithgow T, Daly RJ, Whisstock JC, Song J.

Brief Bioinform. 2019 Nov 27;20(6):2150-2166. doi: 10.1093/bib/bby077.


Keratinocyte-specific ablation of protease-activated receptor 2 prevents gingival inflammation and bone loss in a mouse model of periodontal disease.

Francis N, Ayodele BA, O'Brien-Simpson NM, Birchmeier W, Pike RN, Pagel CN, Mackie EJ.

Cell Microbiol. 2018 Nov;20(11):e12891. doi: 10.1111/cmi.12891. Epub 2018 Jul 31.


Molecular basis for the folding of β-helical autotransporter passenger domains.

Yuan X, Johnson MD, Zhang J, Lo AW, Schembri MA, Wijeyewickrema LC, Pike RN, Huysmans GHM, Henderson IR, Leyton DL.

Nat Commun. 2018 Apr 11;9(1):1395. doi: 10.1038/s41467-018-03593-2.


PROSPERous: high-throughput prediction of substrate cleavage sites for 90 proteases with improved accuracy.

Song J, Li F, Leier A, Marquez-Lago TT, Akutsu T, Haffari G, Chou KC, Webb GI, Pike RN, Hancock J.

Bioinformatics. 2018 Feb 15;34(4):684-687. doi: 10.1093/bioinformatics/btx670.


The Structural Basis for Complement Inhibition by Gigastasin, a Protease Inhibitor from the Giant Amazon Leech.

Pang SS, Wijeyewickrema LC, Hor L, Tan S, Lameignere E, Conway EM, Blom AM, Mohlin FC, Liu X, Payne RJ, Whisstock JC, Pike RN.

J Immunol. 2017 Dec 1;199(11):3883-3891. doi: 10.4049/jimmunol.1700158. Epub 2017 Oct 23.


A T cell-specific knockout reveals an important role for protease-activated receptor 2 in lymphocyte development.

Francis N, Every AL, Ayodele BA, Pike RN, Mackie EJ, Pagel CN.

Int J Biochem Cell Biol. 2017 Nov;92:95-103. doi: 10.1016/j.biocel.2017.09.015. Epub 2017 Sep 22.


Polyphosphate is a novel cofactor for regulation of complement by a serpin, C1 inhibitor.

Wijeyewickrema LC, Lameignere E, Hor L, Duncan RC, Shiba T, Travers RJ, Kapopara PR, Lei V, Smith SA, Kim H, Morrissey JH, Pike RN, Conway EM.

Blood. 2016 Sep 29;128(13):1766-76. doi: 10.1182/blood-2016-02-699561. Epub 2016 Jun 23.


Structural basis for substrate specificity of Helicobacter pylori M17 aminopeptidase.

Modak JK, Rut W, Wijeyewickrema LC, Pike RN, Drag M, Roujeinikova A.

Biochimie. 2016 Feb;121:60-71. doi: 10.1016/j.biochi.2015.11.021. Epub 2015 Dec 1.


Protein unfolding is essential for cleavage within the α-helix of a model protein substrate by the serine protease, thrombin.

Robertson AL, Headey SJ, Ng NM, Wijeyewickrema LC, Scanlon MJ, Pike RN, Bottomley SP.

Biochimie. 2016 Mar;122:227-34. doi: 10.1016/j.biochi.2015.09.021. Epub 2015 Sep 25.


The protease cathepsin L regulates Th17 cell differentiation.

Hou L, Cooley J, Swanson R, Ong PC, Pike RN, Bogyo M, Olson ST, Remold-O'Donnell E.

J Autoimmun. 2015 Dec;65:56-63. doi: 10.1016/j.jaut.2015.08.006. Epub 2015 Sep 3.


Investigation of the mechanism of interaction between Mannose-binding lectin-associated serine protease-2 and complement C4.

Drentin N, Conroy P, Gunzburg MJ, Pike RN, Wijeyewickrema LC.

Mol Immunol. 2015 Oct;67(2 Pt B):287-93. doi: 10.1016/j.molimm.2015.06.011. Epub 2015 Jun 27.


The role of the lys628 (192) residue of the complement protease, c1s, in interacting with Peptide and protein substrates.

Wijeyewickrema LC, Duncan RC, Pike RN.

Front Immunol. 2014 Sep 17;5:444. doi: 10.3389/fimmu.2014.00444. eCollection 2014.


Scabies mite inactive serine proteases are potent inhibitors of the human complement lectin pathway.

Reynolds SL, Pike RN, Mika A, Blom AM, Hofmann A, Wijeyewickrema LC, Kemp D, Fischer K.

PLoS Negl Trop Dis. 2014 May 22;8(5):e2872. doi: 10.1371/journal.pntd.0002872. eCollection 2014 May.


Total synthesis of homogeneous variants of hirudin P6: a post-translationally modified anti-thrombotic leech-derived protein.

Hsieh YS, Wijeyewickrema LC, Wilkinson BL, Pike RN, Payne RJ.

Angew Chem Int Ed Engl. 2014 Apr 7;53(15):3947-51. doi: 10.1002/anie.201310777. Epub 2014 Mar 11.


A molecular basis for the association of the HLA-DRB1 locus, citrullination, and rheumatoid arthritis.

Scally SW, Petersen J, Law SC, Dudek NL, Nel HJ, Loh KL, Wijeyewickrema LC, Eckle SB, van Heemst J, Pike RN, McCluskey J, Toes RE, La Gruta NL, Purcell AW, Reid HH, Thomas R, Rossjohn J.

J Exp Med. 2013 Nov 18;210(12):2569-82. doi: 10.1084/jem.20131241. Epub 2013 Nov 4.


The molecular switches controlling the interaction between complement proteases of the classical and lectin pathways and their substrates.

Pike RN, Wijeyewickrema LC.

Curr Opin Struct Biol. 2013 Dec;23(6):820-7. doi: 10.1016/ Epub 2013 Aug 7. Review.


The x-ray crystal structure of mannose-binding lectin-associated serine proteinase-3 reveals the structural basis for enzyme inactivity associated with the Carnevale, Mingarelli, Malpuech, and Michels (3MC) syndrome.

Yongqing T, Wilmann PG, Reeve SB, Coetzer TH, Smith AI, Whisstock JC, Pike RN, Wijeyewickrema LC.

J Biol Chem. 2013 Aug 2;288(31):22399-407. doi: 10.1074/jbc.M113.483875. Epub 2013 Jun 21. Erratum in: J Biol Chem. 2013 Sep 27;288(39):28307.


A molecular switch governs the interaction between the human complement protease C1s and its substrate, complement C4.

Perry AJ, Wijeyewickrema LC, Wilmann PG, Gunzburg MJ, D'Andrea L, Irving JA, Pang SS, Duncan RC, Wilce JA, Whisstock JC, Pike RN.

J Biol Chem. 2013 May 31;288(22):15821-9. doi: 10.1074/jbc.M113.464545. Epub 2013 Apr 16.


Molecular determinants of the substrate specificity of the complement-initiating protease, C1r.

Wijeyewickrema LC, Yongqing T, Tran TP, Thompson PE, Viljoen JE, Coetzer TH, Duncan RC, Kass I, Buckle AM, Pike RN.

J Biol Chem. 2013 May 31;288(22):15571-80. doi: 10.1074/jbc.M113.451757. Epub 2013 Apr 15.


Thrombin inhibits osteoclast differentiation through a non-proteolytic mechanism.

Sivagurunathan S, Pagel CN, Loh LH, Wijeyewickrema LC, Pike RN, Mackie EJ.

J Mol Endocrinol. 2013 Apr 23;50(3):347-59. doi: 10.1530/JME-12-0177. Print 2013 Jun.


Assembly of the type II secretion system such as found in Vibrio cholerae depends on the novel Pilotin AspS.

Dunstan RA, Heinz E, Wijeyewickrema LC, Pike RN, Purcell AW, Evans TJ, Praszkier J, Robins-Browne RM, Strugnell RA, Korotkov KV, Lithgow T.

PLoS Pathog. 2013 Jan;9(1):e1003117. doi: 10.1371/journal.ppat.1003117. Epub 2013 Jan 10.


PROSPER: an integrated feature-based tool for predicting protease substrate cleavage sites.

Song J, Tan H, Perry AJ, Akutsu T, Webb GI, Whisstock JC, Pike RN.

PLoS One. 2012;7(11):e50300. doi: 10.1371/journal.pone.0050300. Epub 2012 Nov 29.


Structural characterization of the mechanism through which human glutamic acid decarboxylase auto-activates.

Langendorf CG, Tuck KL, Key TL, Fenalti G, Pike RN, Rosado CJ, Wong AS, Buckle AM, Law RH, Whisstock JC.

Biosci Rep. 2013 Jan 11;33(1):137-44. doi: 10.1042/BSR20120111.


Identification of a catalytic exosite for complement component C4 on the serine protease domain of C1s.

Duncan RC, Mohlin F, Taleski D, Coetzer TH, Huntington JA, Payne RJ, Blom AM, Pike RN, Wijeyewickrema LC.

J Immunol. 2012 Sep 1;189(5):2365-73. doi: 10.4049/jimmunol.1201085. Epub 2012 Aug 1.


The X-ray crystal structure of full-length human plasminogen.

Law RH, Caradoc-Davies T, Cowieson N, Horvath AJ, Quek AJ, Encarnacao JA, Steer D, Cowan A, Zhang Q, Lu BG, Pike RN, Smith AI, Coughlin PB, Whisstock JC.

Cell Rep. 2012 Mar 29;1(3):185-90. doi: 10.1016/j.celrep.2012.02.012. Epub 2012 Mar 8.


Novel scabies mite serpins inhibit the three pathways of the human complement system.

Mika A, Reynolds SL, Mohlin FC, Willis C, Swe PM, Pickering DA, Halilovic V, Wijeyewickrema LC, Pike RN, Blom AM, Kemp DJ, Fischer K.

PLoS One. 2012;7(7):e40489. doi: 10.1371/journal.pone.0040489. Epub 2012 Jul 11.


Analysis of Fasciola cathepsin L5 by S2 subsite substitutions and determination of the P1-P4 specificity reveals an unusual preference.

Norbury LJ, Hung A, Beckham S, Pike RN, Spithill TW, Craik CS, Choe Y, Fecondo JV, Smooker PM.

Biochimie. 2012 May;94(5):1119-27. doi: 10.1016/j.biochi.2012.01.011. Epub 2012 Jan 20.


Proteinase-activated receptor-2 is required for normal osteoblast and osteoclast differentiation during skeletal growth and repair.

Georgy SR, Pagel CN, Ghasem-Zadeh A, Zebaze RM, Pike RN, Sims NA, Mackie EJ.

Bone. 2012 Mar;50(3):704-12. doi: 10.1016/j.bone.2011.11.023. Epub 2011 Dec 7.


Effect of O-glycosylation and tyrosine sulfation of leech-derived peptides on binding and inhibitory activity against thrombin.

Hsieh YS, Taleski D, Wilkinson BL, Wijeyewickrema LC, Adams TE, Pike RN, Payne RJ.

Chem Commun (Camb). 2012 Feb 1;48(10):1547-9. doi: 10.1039/c1cc14773k. Epub 2011 Nov 23.


Predicting serpin/protease interactions.

Song J, Matthews AY, Reboul CF, Kaiserman D, Pike RN, Bird PI, Whisstock JC.

Methods Enzymol. 2011;501:237-73. doi: 10.1016/B978-0-12-385950-1.00012-2.


Methods to measure the kinetics of protease inhibition by serpins.

Horvath AJ, Lu BG, Pike RN, Bottomley SP.

Methods Enzymol. 2011;501:223-35. doi: 10.1016/B978-0-12-385950-1.00011-0.


Multiple domains of MASP-2, an initiating complement protease, are required for interaction with its substrate C4.

Duncan RC, Bergström F, Coetzer TH, Blom AM, Wijeyewickrema LC, Pike RN.

Mol Immunol. 2012 Jan;49(4):593-600. doi: 10.1016/j.molimm.2011.10.006. Epub 2011 Nov 8.


Discovery of amino acid motifs for thrombin cleavage and validation using a model substrate.

Ng NM, Pierce JD, Webb GI, Ratnikov BI, Wijeyewickrema LC, Duncan RC, Robertson AL, Bottomley SP, Boyd SE, Pike RN.

Biochemistry. 2011 Dec 6;50(48):10499-507. doi: 10.1021/bi201333g. Epub 2011 Nov 11.


S1 pocket of a bacterially derived subtilisin-like protease underpins effective tissue destruction.

Wong W, Wijeyewickrema LC, Kennan RM, Reeve SB, Steer DL, Reboul C, Smith AI, Pike RN, Rood JI, Whisstock JC, Porter CJ.

J Biol Chem. 2011 Dec 9;286(49):42180-7. doi: 10.1074/jbc.M111.298711. Epub 2011 Oct 11.


Serpins and the complement system.

Beinrohr L, Murray-Rust TA, Dyksterhuis L, Závodszky P, Gál P, Pike RN, Wijeyewickrema LC.

Methods Enzymol. 2011;499:55-75. doi: 10.1016/B978-0-12-386471-0.00004-3.


Mannose-binding lectin serine proteases and associated proteins of the lectin pathway of complement: two genes, five proteins and many functions?

Yongqing T, Drentin N, Duncan RC, Wijeyewickrema LC, Pike RN.

Biochim Biophys Acta. 2012 Jan;1824(1):253-62. doi: 10.1016/j.bbapap.2011.05.021. Epub 2011 Jun 6. Review.


The lysine-specific gingipain of Porphyromonas gingivalis : importance to pathogenicity and potential strategies for inhibition.

Yongqing T, Potempa J, Pike RN, Wijeyewickrema LC.

Adv Exp Med Biol. 2011;712:15-29. doi: 10.1007/978-1-4419-8414-2_2. Review.


Bioinformatic approaches for predicting substrates of proteases.

Song J, Tan H, Boyd SE, Shen H, Mahmood K, Webb GI, Akutsu T, Whisstock JC, Pike RN.

J Bioinform Comput Biol. 2011 Feb;9(1):149-78. Review.


Adult and juvenile Fasciola cathepsin L proteases: different enzymes for different roles.

Norbury LJ, Beckham S, Pike RN, Grams R, Spithill TW, Fecondo JV, Smooker PM.

Biochimie. 2011 Mar;93(3):604-11. doi: 10.1016/j.biochi.2010.12.004. Epub 2010 Dec 16.


The subtilisin-like protease AprV2 is required for virulence and uses a novel disulphide-tethered exosite to bind substrates.

Kennan RM, Wong W, Dhungyel OP, Han X, Wong D, Parker D, Rosado CJ, Law RH, McGowan S, Reeve SB, Levina V, Powers GA, Pike RN, Bottomley SP, Smith AI, Marsh I, Whittington RJ, Whisstock JC, Porter CJ, Rood JI.

PLoS Pathog. 2010 Nov 24;6(11):e1001210. doi: 10.1371/journal.ppat.1001210.


Highlight: the biology of proteolytic systems.

Pike RN, Whisstock JC.

Biol Chem. 2010 Aug;391(8):837. doi: 10.1515/BC.2010.112. No abstract available.


Cathepsin B proteases of flukes: the key to facilitating parasite control?

Smooker PM, Jayaraj R, Pike RN, Spithill TW.

Trends Parasitol. 2010 Oct;26(10):506-14. doi: 10.1016/ Epub 2010 Jun 30. Review.


Vector-based RNA interference of cathepsin B1 in Schistosoma mansoni.

Tchoubrieva EB, Ong PC, Pike RN, Brindley PJ, Kalinna BH.

Cell Mol Life Sci. 2010 Nov;67(21):3739-48. doi: 10.1007/s00018-010-0345-3. Epub 2010 Mar 26.


Protease-activated receptor 2 has pivotal roles in cellular mechanisms involved in experimental periodontitis.

Wong DM, Tam V, Lam R, Walsh KA, Tatarczuch L, Pagel CN, Reynolds EC, O'Brien-Simpson NM, Mackie EJ, Pike RN.

Infect Immun. 2010 Feb;78(2):629-38. doi: 10.1128/IAI.01019-09. Epub 2009 Nov 23.


Characterization of a serine protease homologous to house dust mite group 3 allergens from the scabies mite Sarcoptes scabiei.

Beckham SA, Boyd SE, Reynolds S, Willis C, Johnstone M, Mika A, Simerská P, Wijeyewickrema LC, Smith AI, Kemp DJ, Pike RN, Fischer K.

J Biol Chem. 2009 Dec 4;284(49):34413-22. doi: 10.1074/jbc.M109.061911. Epub 2009 Oct 7.


Proteinase-activated receptor-2 (PAR2) and mouse osteoblasts: regulation of cell function and lack of specificity of PAR2-activating peptides.

Georgy SR, Pagel CN, Wong DM, Sivagurunathan S, Loh LH, Myers DE, Hollenberg MD, Pike RN, Mackie EJ.

Clin Exp Pharmacol Physiol. 2010 Mar;37(3):328-36. doi: 10.1111/j.1440-1681.2009.05294.x. Epub 2009 Sep 21.


Corruption of innate immunity by bacterial proteases.

Potempa J, Pike RN.

J Innate Immun. 2009;1(2):70-87. doi: 10.1159/000181144. Review.


The effects of exosite occupancy on the substrate specificity of thrombin.

Ng NM, Quinsey NS, Matthews AY, Kaiserman D, Wijeyewickrema LC, Bird PI, Thompson PE, Pike RN.

Arch Biochem Biophys. 2009 Sep;489(1-2):48-54. doi: 10.1016/ Epub 2009 Jul 26.


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