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Comparative outcomes of operative relative to medical management of spondylodiscitis accounting for frailty status at presentation.

Alas H, Fernando H, Baker JF, Brown AE, Bortz C, Naessig S, Pierce KE, Ahmad W, Diebo BG, Passias PG.

J Clin Neurosci. 2020 Mar 12. pii: S0967-5868(19)32061-2. doi: 10.1016/j.jocn.2020.03.003. [Epub ahead of print]


Bariatric Surgery Population at Significantly Increased Risk of Spinal Disorders and Surgical Intervention Compared With Morbidly Obese Patients.

Passias PG, Horn SR, Ihejirika YU, Vasques-Montes D, Segreto FA, Bortz CA, Brown AE, Pierce KE, Alas H, Poorman GW, Jalai CM, Wang C, Buza JA 3rd, Steinmetz L, Varlotta CG, Vira S, Diebo BG, Hassanzadeh H, Lafage R, Lafage V.

Clin Spine Surg. 2020 Mar 11. doi: 10.1097/BSD.0000000000000960. [Epub ahead of print]


Operative fusion of patients with metabolic syndrome increases risk for perioperative complications.

Pierce KE, Kapadia BH, Bortz C, Brown A, Alas H, Naessig S, Ahmad W, Vasquez-Montes D, Manning J, Wang E, Maglaras C, Raman T, Protopsaltis TS, Buckland AJ, Passias PG.

J Clin Neurosci. 2020 Feb;72:142-145. doi: 10.1016/j.jocn.2019.12.043. Epub 2019 Dec 30.


Sildenafil Administration in Dogs Heterozygous for a Functional Null Mutation in Pde6a: Suppressed Rod-Mediated ERG Responses and Apparent Retinal Outer Nuclear Layer Thinning.

Pierce KE, Curran PG, Zelinka CP, Fischer AJ, Petersen-Jones SM, Bartoe JT.

Adv Exp Med Biol. 2019;1185:371-376. doi: 10.1007/978-3-030-27378-1_61.


Obesity negatively affects cost efficiency and outcomes following adult spinal deformity surgery.

Brown AE, Alas H, Pierce KE, Bortz CA, Hassanzadeh H, Labaran LA, Puvanesarajah V, Vasquez-Montes D, Wang E, Raman T, Diebo BG, Lafage V, Lafage R, Buckland AJ, Schoenfeld AJ, Gerling MC, Passias PG.

Spine J. 2020 Apr;20(4):512-518. doi: 10.1016/j.spinee.2019.12.012. Epub 2019 Dec 23.


Metabolic Syndrome has a Negative Impact on Cost Utility Following Spine Surgery.

Passias PG, Brown AE, Lebovic J, Pierce KE, Ahmad W, Bortz CA, Alas H, Diebo BG, Buckland AJ.

World Neurosurg. 2020 Mar;135:e500-e504. doi: 10.1016/j.wneu.2019.12.053. Epub 2019 Dec 17.


PROMIS physical health domain scores are related to cervical deformity severity.

Pierce KE, Alas H, Brown AE, Bortz CA, O'Connell B, Vasquez-Montes D, Diebo BG, Lafage R, Lafage V, Buckland AJ, Passias PG.

J Craniovertebr Junction Spine. 2019 Jul-Sep;10(3):179-183. doi: 10.4103/jcvjs.JCVJS_52_19.


The impact of osteotomy grade and location on regional and global alignment following cervical deformity surgery.

Passias PG, Horn SR, Raman T, Brown AE, Lafage V, Lafage R, Smith JS, Bortz CA, Segreto FA, Pierce KE, Alas H, Line BG, Diebo BG, Daniels AH, Kim HJ, Soroceanu A, Mundis GM, Protopsaltis TS, Klineberg EO, Burton DC, Hart RA, Schwab FJ, Bess S, Shaffrey CI, Ames CP; International Spine Study Group.

J Craniovertebr Junction Spine. 2019 Jul-Sep;10(3):160-166. doi: 10.4103/jcvjs.JCVJS_53_19.


Klippel-Feil: A constellation of diagnoses, a contemporary presentation, and recent national trends.

Zhou PL, Poorman GW, Wang C, Pierce KE, Bortz CA, Alas H, Brown AE, Tishelman JC, Janjua MB, Vasquez-Montes D, Moon J, Horn SR, Segreto F, Ihejirika YU, Diebo BG, Passias PG.

J Craniovertebr Junction Spine. 2019 Jul-Sep;10(3):133-138. doi: 10.4103/jcvjs.JCVJS_65_19.


The Influence of Surgical Intervention and Sagittal Alignment on Frailty in Adult Cervical Deformity.

Segreto FA, Passias PG, Brown AE, Horn SR, Bortz CA, Pierce KE, Alas H, Lafage V, Lafage R, Smith JS, Line BG, Diebo BG, Kelly MP, Mundis GM, Protopsaltis TS, Soroceanu A, Kim HJ, Klineberg EO, Burton DC, Hart RA, Schwab FJ, Bess S, Shaffrey CI, Ames CP.

Oper Neurosurg (Hagerstown). 2019 Nov 8. pii: opz331. doi: 10.1093/ons/opz331. [Epub ahead of print]


Ambulatory spine surgery.

Gerling MC, Hale SD, White-Dzuro C, Pierce KE, Naessig SA, Ahmad W, Passias PG.

J Spine Surg. 2019 Sep;5(Suppl 2):S147-S153. doi: 10.21037/jss.2019.09.19. No abstract available.


Does Patient Frailty Status Influence Recovery Following Spinal Fusion for Adult Spinal Deformity?: An Analysis of Patients With 3-Year Follow-up.

Pierce KE, Passias PG, Alas H, Brown AE, Bortz CA, Lafage R, Lafage V, Ames C, Burton DC, Hart R, Hamilton K, Kelly M, Hostin R, Bess S, Klineberg E, Line B, Shaffrey C, Mummaneni P, Smith JS, Schwab FA; International Spine Study Group (ISSG).

Spine (Phila Pa 1976). 2020 Apr 1;45(7):E397-E405. doi: 10.1097/BRS.0000000000003288.


Predictors of Hospital-Acquired Conditions Are Predominately Similar for Spine Surgery and Other Common Elective Surgical Procedures, With Some Key Exceptions.

Horn SR, Pierce KE, Oh C, Segreto FA, Egers M, Bortz C, Vasquez-Montes D, Lafage R, Lafage V, Vira S, Steinmetz L, Ge DH, Buza JA 3rd, Moon J, Diebo BG, Alas H, Brown AE, Shepard NA, Hassanzadeh H, Passias PG.

Global Spine J. 2019 Oct;9(7):717-723. doi: 10.1177/2192568219826083. Epub 2019 Feb 5.


The Impact of Adult Thoracolumbar Spinal Deformities on Standing to Sitting Regional and Segmental Reciprocal Alignment.

Pierce KE, Horn SR, Jain D, Segreto FA, Bortz C, Vasquez-Montes D, Zhou PL, Moon J, Steinmetz L, Varlotta CG, Frangella NJ, Stekas N, Ge DH, Hockley A, Diebo BG, Vira S, Alas H, Brown AE, Lafage R, Lafage V, Schwab FJ, Koller H, Buckland AJ, Gerling MC, Passias PG.

Int J Spine Surg. 2019 Aug 31;13(4):308-316. doi: 10.14444/6042. eCollection 2019 Aug.


Predicting extended operative time and length of inpatient stay in cervical deformity corrective surgery.

Horn SR, Passias PG, Bortz CA, Pierce KE, Lafage V, Lafage R, Brown AE, Alas H, Smith JS, Line B, Deviren V, Mundis GM, Kelly MP, Kim HJ, Protopsaltis T, Daniels AH, Klineberg EO, Burton DC, Hart RA, Schwab FJ, Bess S, Shaffrey CI, Ames CP; International Spine Study Group.

J Clin Neurosci. 2019 Nov;69:206-213. doi: 10.1016/j.jocn.2019.07.064. Epub 2019 Aug 8.


Limited morbidity and possible radiographic benefit of C2 vs. subaxial cervical upper-most instrumented vertebrae.

Passias PG, Bortz CA, Segreto F, Horn S, Pierce KE, Alas H, Brown AE, Lafage R, Lafage V, Smith JS, Line B, Eastlack R, Sciubba DM, Klineberg EO, Soroceanu A, Burton DC, Schwab FJ, Bess S, Shaffrey CI, Ames CP.

J Spine Surg. 2019 Jun;5(2):236-244. doi: 10.21037/jss.2019.06.04.


Association Between Frailty Status and Odontoid Fractures After Traumatic Falls: Investigation of Varying Injury Mechanisms Among 70 Elderly Odontoid Fracture Patients.

Alas H, Segreto FA, Chan HY, Brown AE, Pierce KE, Bortz CA, Horn SR, Varlotta CG, Baker JF, Passias PG.

J Orthop Trauma. 2019 Dec;33(12):e484-e488. doi: 10.1097/BOT.0000000000001597.


Development of a Novel Cervical Deformity Surgical Invasiveness Index.

Passias PG, Horn SR, Soroceanu A, Oh C, Ailon T, Neuman BJ, Lafage V, Lafage R, Smith JS, Line B, Bortz CA, Segreto FA, Brown A, Alas H, Pierce KE, Eastlack RK, Sciubba DM, Protopsaltis TS, Klineberg EO, Burton DC, Hart RA, Schwab FJ, Bess S, Shaffrey CI, Ames CP; International Spine Study Group.

Spine (Phila Pa 1976). 2020 Jan 15;45(2):116-123. doi: 10.1097/BRS.0000000000003175.


Factors influencing length of stay following cervical spine surgery: A comparison of myelopathy and radiculopathy patients.

Pierce KE, Gerling MC, Bortz CA, Alas H, Brown AE, Woo D, Vasquez-Montes D, Ayres EW, Diebo BG, Maglaras C, Janjua MB, Buckland AJ, Fischer CR, Protopsaltis TS, Passias PG.

J Clin Neurosci. 2019 Sep;67:109-113. doi: 10.1016/j.jocn.2019.06.009. Epub 2019 Jun 15.


Short-term Outcomes Following Cervical Laminoplasty and Decompression and Fusion With Instrumentation.

Boniello A, Petrucelli P, Kerbel Y, Horn S, Bortz CA, Brown AE, Pierce KE, Alas H, Khalsa A, Passias P.

Spine (Phila Pa 1976). 2019 Sep 1;44(17):E1018-E1023. doi: 10.1097/BRS.0000000000003057.


Alcoholism as a predictor for pseudarthrosis in primary spine fusion: An analysis of risk factors and 30-day outcomes for 52,402 patients from 2005 to 2013.

Passias PG, Bortz C, Alas H, Segreto FA, Horn SR, Ihejirika YU, Vasquez-Montes D, Pierce KE, Brown AE, Shenoy K, DelSole EM, Johnson B, Oh C, Zhou PL, Deflorimonte C, Dhillon ES, Jankowski PP, Diebo BG, Lafage V, Lafage R, Vira SN, Bendo JA, Goldstein JA, Schwab FJ, Gerling MC.

J Orthop. 2018 Dec 20;16(1):36-40. doi: 10.1016/j.jor.2018.12.011. eCollection 2019 Jan-Feb.


Decreased rates of 30-day perioperative complications following ASD-corrective surgery: A modified Clavien analysis of 3300 patients from 2010 to 2014.

Passias PG, Bortz CA, Pierce KE, Segreto FA, Horn SR, Vasquez-Montes D, Lafage V, Brown AE, Ihejirika Y, Alas H, Varlotta C, Ge DH, Shepard N, Oh C, DelSole EM, Jankowski PP, Hockley A, Diebo BG, Vira SN, Sciubba DM, Raad M, Neuman BJ, Gerling MC.

J Clin Neurosci. 2019 Mar;61:147-152. doi: 10.1016/j.jocn.2018.10.104. Epub 2018 Nov 10.


An association between systemic cyclosporine administration and development of acute bullous keratopathy in cats.

Pierce KE Jr, Wilkie DA, Gemensky-Metzler AJ, Curran PG, Townsend WM, Petersen-Jones SM, Bartoe JT.

Vet Ophthalmol. 2016 Jul;19 Suppl 1:77-85. doi: 10.1111/vop.12367. Epub 2016 Feb 16.


Low-concentration initiator primers improve the amplification of gene targets with high sequence variability.

Pierce KE, Wangh LJ.

Methods Mol Biol. 2015;1275:73-89. doi: 10.1007/978-1-4939-2365-6_5.


Rapid detection of TEM-type extended-spectrum β-lactamase (ESBL) mutations using lights-on/lights-off probes with single-stranded DNA amplification.

Pierce KE, Peter H, Bachmann TT, Volpe C, Mistry R, Rice JE, Wangh LJ.

J Mol Diagn. 2013 May;15(3):291-8. doi: 10.1016/j.jmoldx.2013.02.002. Epub 2013 Mar 19.


Rapid detection of sequence variation in Clostridium difficile genes using LATE-PCR with multiple mismatch-tolerant hybridization probes.

Pierce KE, Khan H, Mistry R, Goldenberg SD, French GL, Wangh LJ.

J Microbiol Methods. 2012 Nov;91(2):269-75. doi: 10.1016/j.mimet.2012.08.018. Epub 2012 Sep 7.


Bilateral uveal metastasis of a subcutaneous fibrosarcoma in a cat.

Mowat FM, Langohr IM, Bilyk O, Koterbay A, Pierce KE, Petersen-Jones SM.

Vet Ophthalmol. 2012 Nov;15(6):391-7. doi: 10.1111/j.1463-5224.2012.00993.x. Epub 2012 Feb 23.


LATE-PCR and allied technologies: real-time detection strategies for rapid, reliable diagnosis from single cells.

Pierce KE, Wangh LJ.

Methods Mol Biol. 2011;688:47-66. doi: 10.1007/978-1-60761-947-5_5.


Pan-serotypic detection of foot-and-mouth disease virus by RT linear-after-the-exponential PCR.

Reid SM, Pierce KE, Mistry R, Bharya S, Dukes JP, Volpe C, Wangh LJ, King DP.

Mol Cell Probes. 2010 Oct;24(5):250-5. doi: 10.1016/j.mcp.2010.04.004. Epub 2010 Apr 28.


Design and optimization of a novel reverse transcription linear-after-the-exponential PCR for the detection of foot-and-mouth disease virus.

Pierce KE, Mistry R, Reid SM, Bharya S, Dukes JP, Hartshorn C, King DP, Wangh LJ.

J Appl Microbiol. 2010 Jul;109(1):180-9. doi: 10.1111/j.1365-2672.2009.04640.x. Epub 2009 Nov 28.


The influence of ethnicity on thermoregulation after acute cold exposure.

Farnell GS, Pierce KE, Collinsworth TA, Murray LK, Demes RN, Juvancic-Heltzel JA, Glickman EL.

Wilderness Environ Med. 2008 Winter;19(4):238-44. doi: 10.1580/07-WEME-OR-138.1.


Monoplex/multiplex linear-after-the-exponential-PCR assays combined with PrimeSafe and Dilute-'N'-Go sequencing.

Rice JE, Sanchez JA, Pierce KE, Reis AH Jr, Osborne A, Wangh LJ.

Nat Protoc. 2007;2(10):2429-38.


Two-temperature LATE-PCR endpoint genotyping.

Sanchez JA, Abramowitz JD, Salk JJ, Reis AH Jr, Rice JE, Pierce KE, Wangh LJ.

BMC Biotechnol. 2006 Dec 4;6:44.


Direct amplification of single-stranded DNA for pyrosequencing using linear-after-the-exponential (LATE)-PCR.

Salk JJ, Sanchez JA, Pierce KE, Rice JE, Soares KC, Wangh LJ.

Anal Biochem. 2006 Jun 1;353(1):124-32. Epub 2006 Feb 28.


Linear-After-The-Exponential (LATE)-PCR: primer design criteria for high yields of specific single-stranded DNA and improved real-time detection.

Pierce KE, Sanchez JA, Rice JE, Wangh LJ.

Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8609-14. Epub 2005 Jun 3.


Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis.

Sanchez JA, Pierce KE, Rice JE, Wangh LJ.

Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):1933-8. Epub 2004 Feb 9. Erratum in: Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):2083.


Preimplantation genetic diagnosis for a couple with recurrent pregnancy loss and triploidy.

Bar-Ami S, Seibel MM, Pierce KE, Zilberstein M.

Birth Defects Res A Clin Mol Teratol. 2003 Nov;67(11):946-50.


Effectiveness and limitations of uracil-DNA glycosylases in sensitive real-time PCR assays.

Pierce KE, Wangh LJ.

Biotechniques. 2004 Jan;36(1):44-6, 48. No abstract available.


Detection of cystic fibrosis alleles from single cells using molecular beacons and a novel method of asymmetric real-time PCR.

Pierce KE, Rice JE, Sanchez JA, Wangh LJ.

Mol Hum Reprod. 2003 Dec;9(12):815-20.


Real-time PCR with molecular beacons provides a highly accurate assay for detection of Tay-Sachs alleles in single cells.

Rice JE, Sanchez JA, Pierce KE, Wangh LJ.

Prenat Diagn. 2002 Dec;22(12):1130-4.


QuantiLyse: reliable DNA amplification from single cells.

Pierce KE, Rice JE, Sanchez JA, Wangh LJ.

Biotechniques. 2002 May;32(5):1106-11.


Real-time PCR using molecular beacons for accurate detection of the Y chromosome in single human blastomeres.

Pierce KE, Rice JE, Sanchez JA, Brenner C, Wangh LJ.

Mol Hum Reprod. 2000 Dec;6(12):1155-64.


Preimplantation genetic diagnosis of chromosome balance in embryos from a patient with a balanced reciprocal translocation.

Pierce KE, Fitzgerald LM, Seibel MM, Zilberstein M.

Mol Hum Reprod. 1998 Feb;4(2):167-72.


Preimplantation development of mouse and human embryos biopsied at cleavage stages using a modified displacement technique.

Pierce KE, Michalopoulos J, Kiessling AA, Seibel MM, Zilberstein M.

Hum Reprod. 1997 Feb;12(2):351-6.


Temporal pattern of synthesis of the mouse cortical granule protein, p75, during oocyte growth and maturation.

Pierce KE, Grunvald EL, Schultz RM, Kopf GS.

Dev Biol. 1992 Jul;152(1):145-51.


Characterization and localization of a mouse egg cortical granule antigen prior to and following fertilization or egg activation.

Pierce KE, Siebert MC, Kopf GS, Schultz RM, Calarco PG.

Dev Biol. 1990 Oct;141(2):381-92.


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