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Common principles and best practices for engineering microbiomes.

Lawson CE, Harcombe WR, Hatzenpichler R, Lindemann SR, Löffler FE, O'Malley MA, García Martín H, Pfleger BF, Raskin L, Venturelli OS, Weissbrodt DG, Noguera DR, McMahon KD.

Nat Rev Microbiol. 2019 Dec;17(12):725-741. doi: 10.1038/s41579-019-0255-9. Epub 2019 Sep 23. Review.


Growth-coupled bioconversion of levulinic acid to butanone.

Mehrer CR, Rand JM, Incha MR, Cook TB, Demir B, Motagamwala AH, Kim D, Dumesic JA, Pfleger BF.

Metab Eng. 2019 Sep;55:92-101. doi: 10.1016/j.ymben.2019.06.003. Epub 2019 Jun 19.


Leveraging synthetic biology for producing bioactive polyketides and non-ribosomal peptides in bacterial heterologous hosts.

Cook TB, Pfleger BF.

Medchemcomm. 2019 Apr 25;10(5):668-681. doi: 10.1039/c9md00055k. eCollection 2019 May 1. Review.


Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals.

Yan Q, Pfleger BF.

Metab Eng. 2019 Apr 22. pii: S1096-7176(19)30113-2. doi: 10.1016/j.ymben.2019.04.009. [Epub ahead of print] Review.


Regulatory Tools for Controlling Gene Expression in Cyanobacteria.

Gordon GC, Pfleger BF.

Adv Exp Med Biol. 2018;1080:281-315. doi: 10.1007/978-981-13-0854-3_12. Review.


Highly Active C8-Acyl-ACP Thioesterase Variant Isolated by a Synthetic Selection Strategy.

Hernández Lozada NJ, Lai RY, Simmons TR, Thomas KA, Chowdhury R, Maranas CD, Pfleger BF.

ACS Synth Biol. 2018 Sep 21;7(9):2205-2215. doi: 10.1021/acssynbio.8b00215. Epub 2018 Aug 21.


Anaerobic production of medium-chain fatty alcohols via a β-reduction pathway.

Mehrer CR, Incha MR, Politz MC, Pfleger BF.

Metab Eng. 2018 Jul;48:63-71. doi: 10.1016/j.ymben.2018.05.011. Epub 2018 May 25.


Light-optimized growth of cyanobacterial cultures: Growth phases and productivity of biomass and secreted molecules in light-limited batch growth.

Clark RL, McGinley LL, Purdy HM, Korosh TC, Reed JL, Root TW, Pfleger BF.

Metab Eng. 2018 May;47:230-242. doi: 10.1016/j.ymben.2018.03.017. Epub 2018 Mar 27.


Inhibition of Cyanobacterial Growth on a Municipal Wastewater Sidestream Is Impacted by Temperature.

Korosh TC, Dutcher A, Pfleger BF, McMahon KD.

mSphere. 2018 Feb 28;3(1). pii: e00538-17. doi: 10.1128/mSphere.00538-17. eCollection 2018 Jan-Feb.


Computational Redesign of Acyl-ACP Thioesterase with Improved Selectivity toward Medium-Chain-Length Fatty Acids.

Grisewood MJ, Hernandez Lozada NJ, Thoden JB, Gifford NP, Mendez-Perez D, Schoenberger HA, Allan MF, Floy ME, Lai RY, Holden HM, Pfleger BF, Maranas CD.

ACS Catal. 2017 Jun 2;7(6):3837-3849. doi: 10.1021/acscatal.7b00408. Epub 2017 Apr 20.


Distinct and redundant functions of three homologs of RNase III in the cyanobacterium Synechococcus sp. strain PCC 7002.

Gordon GC, Cameron JC, Pfleger BF.

Nucleic Acids Res. 2018 Feb 28;46(4):1984-1997. doi: 10.1093/nar/gky041.


High-CO2 Requirement as a Mechanism for the Containment of Genetically Modified Cyanobacteria.

Clark RL, Gordon GC, Bennett NR, Lyu H, Root TW, Pfleger BF.

ACS Synth Biol. 2018 Feb 16;7(2):384-391. doi: 10.1021/acssynbio.7b00377. Epub 2018 Jan 12.


Transcription control engineering and applications in synthetic biology.

Engstrom MD, Pfleger BF.

Synth Syst Biotechnol. 2017 Oct 4;2(3):176-191. doi: 10.1016/j.synbio.2017.09.003. eCollection 2017 Sep. Review.


Genetic tools for reliable gene expression and recombineering in Pseudomonas putida.

Cook TB, Rand JM, Nurani W, Courtney DK, Liu SA, Pfleger BF.

J Ind Microbiol Biotechnol. 2018 Jul;45(7):517-527. doi: 10.1007/s10295-017-2001-5. Epub 2018 Jan 3.


Genome sequence and analysis of Escherichia coli production strain LS5218.

Rand JM, Gordon GC, Mehrer CR, Pfleger BF.

Metab Eng Commun. 2017 Nov 2;5:78-83. doi: 10.1016/j.meteno.2017.10.001. eCollection 2017 Dec.


Engineering photosynthetic production of L-lysine.

Korosh TC, Markley AL, Clark RL, McGinley LL, McMahon KD, Pfleger BF.

Metab Eng. 2017 Nov;44:273-283. doi: 10.1016/j.ymben.2017.10.010. Epub 2017 Oct 28.


Flux Balance Analysis Indicates that Methane Is the Lowest Cost Feedstock for Microbial Cell Factories.

Comer AD, Long MR, Reed JL, Pfleger BF.

Metab Eng Commun. 2017 Dec;5:26-33. doi: 10.1016/j.meteno.2017.07.002. Epub 2017 Jul 10.


A metabolic pathway for catabolizing levulinic acid in bacteria.

Rand JM, Pisithkul T, Clark RL, Thiede JM, Mehrer CR, Agnew DE, Campbell CE, Markley AL, Price MN, Ray J, Wetmore KM, Suh Y, Arkin AP, Deutschbauer AM, Amador-Noguez D, Pfleger BF.

Nat Microbiol. 2017 Dec;2(12):1624-1634. doi: 10.1038/s41564-017-0028-z. Epub 2017 Sep 25.


Editorial overview: Energy biotechnology.

Pfleger BF, Banta S.

Curr Opin Biotechnol. 2017 Jun;45:v-viii. doi: 10.1016/j.copbio.2017.05.002. Epub 2017 May 18. No abstract available.


RNA Sequencing Identifies New RNase III Cleavage Sites in Escherichia coli and Reveals Increased Regulation of mRNA.

Gordon GC, Cameron JC, Pfleger BF.

MBio. 2017 Mar 28;8(2). pii: e00128-17. doi: 10.1128/mBio.00128-17.


Reassessing Escherichia coli as a cell factory for biofuel production.

Wang C, Pfleger BF, Kim SW.

Curr Opin Biotechnol. 2017 Jun;45:92-103. doi: 10.1016/j.copbio.2017.02.010. Epub 2017 Mar 11. Review.


A roadmap for the synthesis of separation networks for the recovery of bio-based chemicals: Matching biological and process feasibility.

Yenkie KM, Wu W, Clark RL, Pfleger BF, Root TW, Maravelias CT.

Biotechnol Adv. 2016 Dec;34(8):1362-1383. doi: 10.1016/j.biotechadv.2016.10.003. Epub 2016 Oct 15. Review.


CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002.

Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger BF.

Metab Eng. 2016 Nov;38:170-179. doi: 10.1016/j.ymben.2016.07.007. Epub 2016 Jul 29.


Functional genomics analysis of free fatty acid production under continuous phosphate limiting conditions.

Youngquist JT, Korosh TC, Pfleger BF.

J Ind Microbiol Biotechnol. 2017 May;44(4-5):759-772. doi: 10.1007/s10295-016-1846-3. Epub 2016 Oct 13.


Microbes paired for biological gas-to-liquids (Bio-GTL) process.

Pfleger BF.

Proc Natl Acad Sci U S A. 2016 Apr 5;113(14):3717-9. doi: 10.1073/pnas.1601926113. Epub 2016 Mar 16. No abstract available.


A transcription activator-like effector (TALE) induction system mediated by proteolysis.

Copeland MF, Politz MC, Johnson CB, Markley AL, Pfleger BF.

Nat Chem Biol. 2016 Apr;12(4):254-60. doi: 10.1038/nchembio.2021. Epub 2016 Feb 8.


Biological synthesis unbounded?

Pfleger BF, Prather KL.

Nat Biotechnol. 2015 Nov;33(11):1148-9. doi: 10.1038/nbt.3399. No abstract available.


Efflux systems in bacteria and their metabolic engineering applications.

Jones CM, Hernández Lozada NJ, Pfleger BF.

Appl Microbiol Biotechnol. 2015 Nov;99(22):9381-93. doi: 10.1007/s00253-015-6963-9. Epub 2015 Sep 12. Review.


Construction of new synthetic biology tools for the control of gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002.

Zess EK, Begemann MB, Pfleger BF.

Biotechnol Bioeng. 2016 Feb;113(2):424-32. doi: 10.1002/bit.25713. Epub 2015 Sep 3.


Solvent-enabled nonenyzmatic sugar production from biomass for chemical and biological upgrading.

Luterbacher JS, Alonso DM, Rand JM, Questell-Santiago YM, Yeap JH, Pfleger BF, Dumesic JA.

ChemSusChem. 2015 Apr 24;8(8):1317-22. doi: 10.1002/cssc.201403418. Epub 2015 Mar 17.


Metabolic engineering strategies for microbial synthesis of oleochemicals.

Pfleger BF, Gossing M, Nielsen J.

Metab Eng. 2015 May;29:1-11. doi: 10.1016/j.ymben.2015.01.009. Epub 2015 Feb 7. Review.


Genetic and genomic analysis of RNases in model cyanobacteria.

Cameron JC, Gordon GC, Pfleger BF.

Photosynth Res. 2015 Oct;126(1):171-83. doi: 10.1007/s11120-015-0076-2. Epub 2015 Jan 17.


Synthetic biology toolbox for controlling gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002.

Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF.

ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. Epub 2014 Sep 25.


A desaturase gene involved in the formation of 1,14-nonadecadiene in Synechococcus sp. strain PCC 7002.

Mendez-Perez D, Herman NA, Pfleger BF.

Appl Environ Microbiol. 2014 Oct;80(19):6073-9. doi: 10.1128/AEM.01615-14. Epub 2014 Jul 25.


Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes.

Copeland MF, Politz MC, Pfleger BF.

Curr Opin Biotechnol. 2014 Oct;29:46-54. doi: 10.1016/j.copbio.2014.02.010. Epub 2014 Mar 12. Review.


Nonenzymatic sugar production from biomass using biomass-derived γ-valerolactone.

Luterbacher JS, Rand JM, Alonso DM, Han J, Youngquist JT, Maravelias CT, Pfleger BF, Dumesic JA.

Science. 2014 Jan 17;343(6168):277-80. doi: 10.1126/science.1246748.


Production of medium chain length fatty alcohols from glucose in Escherichia coli.

Youngquist JT, Schumacher MH, Rose JP, Raines TC, Politz MC, Copeland MF, Pfleger BF.

Metab Eng. 2013 Nov;20:177-86. doi: 10.1016/j.ymben.2013.10.006. Epub 2013 Oct 17.


An organic acid based counter selection system for cyanobacteria.

Begemann MB, Zess EK, Walters EM, Schmitt EF, Markley AL, Pfleger BF.

PLoS One. 2013 Oct 1;8(10):e76594. doi: 10.1371/journal.pone.0076594. eCollection 2013.


By-passing the refinery for production of high-value BTX derivatives.

Pfleger BF.

Biotechnol J. 2013 Dec;8(12):1375-6. doi: 10.1002/biot.201300251. Epub 2013 Jul 29. No abstract available.


Free fatty acid production in Escherichia coli under phosphate-limited conditions.

Youngquist JT, Rose JP, Pfleger BF.

Appl Microbiol Biotechnol. 2013 Jun;97(11):5149-59. doi: 10.1007/s00253-013-4911-0. Epub 2013 Apr 26.


Microbial production of fatty acid-derived fuels and chemicals.

Lennen RM, Pfleger BF.

Curr Opin Biotechnol. 2013 Dec;24(6):1044-53. doi: 10.1016/j.copbio.2013.02.028. Epub 2013 Mar 28. Review.


Modulating membrane composition alters free fatty acid tolerance in Escherichia coli.

Lennen RM, Pfleger BF.

PLoS One. 2013;8(1):e54031. doi: 10.1371/journal.pone.0054031. Epub 2013 Jan 21.


Artificial repressors for controlling gene expression in bacteria.

Politz MC, Copeland MF, Pfleger BF.

Chem Commun (Camb). 2013 May 14;49(39):4325-7. doi: 10.1039/c2cc37107c. Epub 2012 Dec 10.


Engineering Escherichia coli for production of C₁₂-C₁₄ polyhydroxyalkanoate from glucose.

Agnew DE, Stevermer AK, Youngquist JT, Pfleger BF.

Metab Eng. 2012 Nov;14(6):705-13. doi: 10.1016/j.ymben.2012.08.003.


Identification of transport proteins involved in free fatty acid efflux in Escherichia coli.

Lennen RM, Politz MG, Kruziki MA, Pfleger BF.

J Bacteriol. 2013 Jan;195(1):135-44. doi: 10.1128/JB.01477-12. Epub 2012 Oct 26.


Engineering Escherichia coli to synthesize free fatty acids.

Lennen RM, Pfleger BF.

Trends Biotechnol. 2012 Dec;30(12):659-67. doi: 10.1016/j.tibtech.2012.09.006. Epub 2012 Oct 23. Review.


A translation-coupling DNA cassette for monitoring protein translation in Escherichia coli.

Mendez-Perez D, Gunasekaran S, Orler VJ, Pfleger BF.

Metab Eng. 2012 Jul;14(4):298-305. doi: 10.1016/j.ymben.2012.04.005. Epub 2012 May 3.


Isolation of improved free fatty acid overproducing strains of Escherichia coli via Nile red based high-throughput screening.

Hoover SW, Youngquist JT, Angart PA, Withers ST, Lennen RM, Pfleger BF.

Environ Prog Sustain Energy. 2012 Apr;31(1):17-23. doi: 10.1002/ep.10599. Epub 2011 Nov 17.


Functional and structural analysis of the siderophore synthetase AsbB through reconstitution of the petrobactin biosynthetic pathway from Bacillus anthracis.

Nusca TD, Kim Y, Maltseva N, Lee JY, Eschenfeldt W, Stols L, Schofield MM, Scaglione JB, Dixon SD, Oves-Costales D, Challis GL, Hanna PC, Pfleger BF, Joachimiak A, Sherman DH.

J Biol Chem. 2012 May 4;287(19):16058-72. doi: 10.1074/jbc.M112.359349. Epub 2012 Mar 9.


Kinetic modeling of free fatty acid production in Escherichia coli based on continuous cultivation of a plasmid free strain.

Youngquist JT, Lennen RM, Ranatunga DR, Bothfeld WH, Marner WD 2nd, Pfleger BF.

Biotechnol Bioeng. 2012 Jun;109(6):1518-27. doi: 10.1002/bit.24420. Epub 2012 Jan 6.


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