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Items: 1 to 50 of 135

1.

Identification of a piscine reovirus-related pathogen in proliferative darkening syndrome (PDS) infected brown trout (Salmo trutta fario) using a next-generation technology detection pipeline.

Kuehn R, Stoeckle BC, Young M, Popp L, Taeubert JE, Pfaffl MW, Geist J.

PLoS One. 2018 Oct 22;13(10):e0206164. doi: 10.1371/journal.pone.0206164. eCollection 2018.

2.

Highlights of the São Paulo ISEV workshop on extracellular vesicles in cross-kingdom communication.

Soares RP, Xander P, Costa AO, Marcilla A, Menezes-Neto A, Del Portillo H, Witwer K, Wauben M, Nolte-T Hoen E, Olivier M, Criado MF, da Silva LLP, Abdel Baqui MM, Schenkman S, Colli W, Alves MJM, Ferreira KS, Puccia R, Nejsum P, Riesbeck K, Stensballe A, Hansen EP, Jaular LM, Øvstebø R, de la Canal L, Bergese P, Pereira-Chioccola V, Pfaffl MW, Fritz J, Gho YS, Torrecilhas AC.

J Extracell Vesicles. 2017 Nov 26;6(1):1407213. doi: 10.1080/20013078.2017.1407213. eCollection 2017.

3.

Changes in the microRNA expression profile during blood storage.

Haberberger A, Kirchner B, Riedmaier I, Henschler R, Wichmann C, Buhmann R, Pfaffl MW.

BMJ Open Sport Exerc Med. 2018 Jul 5;4(1):e000354. doi: 10.1136/bmjsem-2018-000354. eCollection 2018.

4.

Shisha microbiota: the good, the bad and the not so ugly.

Hani J, Abdel Nour G, Matta J, Jazzar B, Pfaffl MW, Hanna-Wakim L, Abdel Nour AM.

BMC Res Notes. 2018 Jul 6;11(1):446. doi: 10.1186/s13104-018-3553-9.

5.

Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next-generation sequencing.

Buschmann D, Kirchner B, Hermann S, Märte M, Wurmser C, Brandes F, Kotschote S, Bonin M, Steinlein OK, Pfaffl MW, Schelling G, Reithmair M.

J Extracell Vesicles. 2018 Jun 4;7(1):1481321. doi: 10.1080/20013078.2018.1481321. eCollection 2018.

6.

New surveillance concepts in food safety in meat producing animals: the advantage of high throughput 'omics' technologies - A review.

Pfaffl MW, Riedmaier-Sprenzel I.

Asian-Australas J Anim Sci. 2018 Jul;31(7):1062-1071. doi: 10.5713/ajas.18.0155. Epub 2018 May 31.

7.

Changes in the expression of prostaglandin family members in bovine corpus luteum during the estrous cycle and pregnancy.

Berisha B, Schams D, Rodler D, Sinowatz F, Pfaffl MW.

Mol Reprod Dev. 2018 Jul;85(7):622-634. doi: 10.1002/mrd.22999. Epub 2018 Jul 24.

PMID:
29877057
8.

Growth Hormone Secretion Patterns in German Landrace (DL) Fetuses and Piglets Compared to DL Piglets with Inherited 1,25-Dihydroxyvitamin D3 Deficiency.

Mielenz M, Pfaffl MW, Schlumbohm C, Harmeyer J, Parvizi N.

Nutrients. 2018 May 15;10(5). pii: E617. doi: 10.3390/nu10050617.

9.

Stimulated enrichment of Clostridium difficile specific IgA in mature cow's milk.

Schmautz C, Hillreiner M, Ballweg I, Pfaffl MW, Kliem H.

PLoS One. 2018 Apr 25;13(4):e0195275. doi: 10.1371/journal.pone.0195275. eCollection 2018.

10.

Glucocorticoid receptor overexpression slightly shifts microRNA expression patterns in triple-negative breast cancer.

Buschmann D, González R, Kirchner B, Mazzone C, Pfaffl MW, Schelling G, Steinlein O, Reithmair M.

Int J Oncol. 2018 Jun;52(6):1765-1776. doi: 10.3892/ijo.2018.4336. Epub 2018 Mar 27.

11.

Impact of preimplantational oral low-dose estradiol-17β exposure on the endometrium: The role of miRNA.

Flöter VL, Lorenz AK, Kirchner B, Pfaffl MW, Bauersachs S, Ulbrich SE.

Mol Reprod Dev. 2018 May;85(5):417-426. doi: 10.1002/mrd.22975. Epub 2018 May 9.

PMID:
29542831
12.

Grass pollen production and group V allergen content of agriculturally relevant species and cultivars.

Jung S, Estrella N, Pfaffl MW, Hartmann S, Handelshauser E, Menzel A.

PLoS One. 2018 Mar 12;13(3):e0193958. doi: 10.1371/journal.pone.0193958. eCollection 2018.

13.

The Dynamics of microRNA Transcriptome in Bovine Corpus Luteum during Its Formation, Function, and Regression.

Gecaj RM, Schanzenbach CI, Kirchner B, Pfaffl MW, Riedmaier I, Tweedie-Cullen RY, Berisha B.

Front Genet. 2017 Dec 15;8:213. doi: 10.3389/fgene.2017.00213. eCollection 2017.

14.

Gene expression profiling in pbMEC - in search of molecular biomarkers to predict immunoglobulin production in bovine milk.

Hillreiner M, Schmautz C, Ballweg I, Korenkova V, Pfaffl MW, Kliem H.

BMC Vet Res. 2017 Nov 29;13(1):369. doi: 10.1186/s12917-017-1293-z.

15.

Establishment of a 3D cell culture model of primary bovine mammary epithelial cells extracted from fresh milk.

Hillreiner M, Müller NI, Koch HM, Schmautz C, Küster B, Pfaffl MW, Kliem H.

In Vitro Cell Dev Biol Anim. 2017 Sep;53(8):706-720. doi: 10.1007/s11626-017-0169-7. Epub 2017 Jun 22.

PMID:
28643224
16.

Cellular and extracellular miRNAs are blood-compartment-specific diagnostic targets in sepsis.

Reithmair M, Buschmann D, Märte M, Kirchner B, Hagl D, Kaufmann I, Pfob M, Chouker A, Steinlein OK, Pfaffl MW, Schelling G.

J Cell Mol Med. 2017 Oct;21(10):2403-2411. doi: 10.1111/jcmm.13162. Epub 2017 Apr 6.

17.

Obstacles and opportunities in the functional analysis of extracellular vesicle RNA - an ISEV position paper.

Mateescu B, Kowal EJ, van Balkom BW, Bartel S, Bhattacharyya SN, Buzás EI, Buck AH, de Candia P, Chow FW, Das S, Driedonks TA, Fernández-Messina L, Haderk F, Hill AF, Jones JC, Van Keuren-Jensen KR, Lai CP, Lässer C, Liegro ID, Lunavat TR, Lorenowicz MJ, Maas SL, Mäger I, Mittelbrunn M, Momma S, Mukherjee K, Nawaz M, Pegtel DM, Pfaffl MW, Schiffelers RM, Tahara H, Théry C, Tosar JP, Wauben MH, Witwer KW, Nolte-'t Hoen EN.

J Extracell Vesicles. 2017 Mar 7;6(1):1286095. doi: 10.1080/20013078.2017.1286095. eCollection 2017.

18.

EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research.

EV-TRACK Consortium, Van Deun J, Mestdagh P, Agostinis P, Akay Ö, Anand S, Anckaert J, Martinez ZA, Baetens T, Beghein E, Bertier L, Berx G, Boere J, Boukouris S, Bremer M, Buschmann D, Byrd JB, Casert C, Cheng L, Cmoch A, Daveloose D, De Smedt E, Demirsoy S, Depoorter V, Dhondt B, Driedonks TA, Dudek A, Elsharawy A, Floris I, Foers AD, Gärtner K, Garg AD, Geeurickx E, Gettemans J, Ghazavi F, Giebel B, Kormelink TG, Hancock G, Helsmoortel H, Hill AF, Hyenne V, Kalra H, Kim D, Kowal J, Kraemer S, Leidinger P, Leonelli C, Liang Y, Lippens L, Liu S, Lo Cicero A, Martin S, Mathivanan S, Mathiyalagan P, Matusek T, Milani G, Monguió-Tortajada M, Mus LM, Muth DC, Németh A, Nolte-'t Hoen EN, O'Driscoll L, Palmulli R, Pfaffl MW, Primdal-Bengtson B, Romano E, Rousseau Q, Sahoo S, Sampaio N, Samuel M, Scicluna B, Soen B, Steels A, Swinnen JV, Takatalo M, Thaminy S, Théry C, Tulkens J, Van Audenhove I, van der Grein S, Van Goethem A, van Herwijnen MJ, Van Niel G, Van Roy N, Van Vliet AR, Vandamme N, Vanhauwaert S, Vergauwen G, Verweij F, Wallaert A, Wauben M, Witwer KW, Zonneveld MI, De Wever O, Vandesompele J, Hendrix A.

Nat Methods. 2017 Feb 28;14(3):228-232. doi: 10.1038/nmeth.4185.

PMID:
28245209
19.

Can milk cell or skim milk miRNAs be used as biomarkers for early pregnancy detection in cattle?

Schanzenbach CI, Kirchner B, Ulbrich SE, Pfaffl MW.

PLoS One. 2017 Feb 24;12(2):e0172220. doi: 10.1371/journal.pone.0172220. eCollection 2017.

20.

Effect of Perilla frutescens Extracts on Porcine Jejunal Epithelial Cells.

Kaufmann CM, Letzel T, Grassmann J, Pfaffl MW.

Phytother Res. 2017 Feb;31(2):303-311. doi: 10.1002/ptr.5750. Epub 2016 Dec 13.

PMID:
27958644
21.

Expression pattern of HIF1alpha and vasohibins during follicle maturation and corpus luteum function in the bovine ovary.

Berisha B, Schams D, Rodler D, Sinowatz F, Pfaffl MW.

Reprod Domest Anim. 2017 Feb;52(1):130-139. doi: 10.1111/rda.12867. Epub 2016 Nov 11.

PMID:
27862406
22.
23.

Reply.

Ege MJ, Pfaffl MW.

J Allergy Clin Immunol. 2016 Sep;138(3):938-939. doi: 10.1016/j.jaci.2016.04.040. Epub 2016 Jul 12. No abstract available.

PMID:
27417021
24.

Toward reliable biomarker signatures in the age of liquid biopsies - how to standardize the small RNA-Seq workflow.

Buschmann D, Haberberger A, Kirchner B, Spornraft M, Riedmaier I, Schelling G, Pfaffl MW.

Nucleic Acids Res. 2016 Jul 27;44(13):5995-6018. doi: 10.1093/nar/gkw545. Epub 2016 Jun 17.

25.

Effect of the Ketone Body Beta-Hydroxybutyrate on the Innate Defense Capability of Primary Bovine Mammary Epithelial Cells.

Hillreiner M, Flinspach C, Pfaffl MW, Kliem H.

PLoS One. 2016 Jun 16;11(6):e0157774. doi: 10.1371/journal.pone.0157774. eCollection 2016.

26.

Comparison of the immune competence of Turopolje, German Landrace × Turopolje, and German Landrace × Pietrain pigs after PRRSV vaccination.

Ballweg IC, Frölich K, Fandrey E, Kliem H, Pfaffl MW.

Vet Immunol Immunopathol. 2016 Jun;174:35-44. doi: 10.1016/j.vetimm.2016.04.003. Epub 2016 Apr 5.

PMID:
27185261
27.

The potential of circulating extracellular small RNAs (smexRNA) in veterinary diagnostics-Identifying biomarker signatures by multivariate data analysis.

Melanie S, Benedikt K, Pfaffl MW, Irmgard R.

Biomol Detect Quantif. 2015 Sep 19;5:15-22. doi: 10.1016/j.bdq.2015.08.001. eCollection 2015 Sep.

28.

Guest editor's introduction for BDQ special issue: 'Advanced Molecular Diagnostics for Biomarker Discovery'.

Pfaffl MW.

Biomol Detect Quantif. 2015 Oct 8;5:1-2. doi: 10.1016/j.bdq.2015.09.003. eCollection 2015 Sep. No abstract available.

29.

How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.

Svec D, Tichopad A, Novosadova V, Pfaffl MW, Kubista M.

Biomol Detect Quantif. 2015 Mar 11;3:9-16. doi: 10.1016/j.bdq.2015.01.005. eCollection 2015 Mar.

30.

Inhibition of fat cell differentiation in 3T3-L1 pre-adipocytes by all-trans retinoic acid: Integrative analysis of transcriptomic and phenotypic data.

Stoecker K, Sass S, Theis FJ, Hauner H, Pfaffl MW.

Biomol Detect Quantif. 2016 Nov 21;11:31-44. doi: 10.1016/j.bdq.2016.11.001. eCollection 2017 Mar.

31.

microRNA in native and processed cow's milk and its implication for the farm milk effect on asthma.

Kirchner B, Pfaffl MW, Dumpler J, von Mutius E, Ege MJ.

J Allergy Clin Immunol. 2016 Jun;137(6):1893-1895.e13. doi: 10.1016/j.jaci.2015.10.028. Epub 2015 Dec 19. No abstract available.

PMID:
26707195
32.

Tumor Necrosis Factor Alpha and Insulin-Like Growth Factor 1 Induced Modifications of the Gene Expression Kinetics of Differentiating Skeletal Muscle Cells.

Meyer SU, Krebs S, Thirion C, Blum H, Krause S, Pfaffl MW.

PLoS One. 2015 Oct 8;10(10):e0139520. doi: 10.1371/journal.pone.0139520. eCollection 2015.

33.

Integrative Analysis of MicroRNA and mRNA Data Reveals an Orchestrated Function of MicroRNAs in Skeletal Myocyte Differentiation in Response to TNF-α or IGF1.

Meyer SU, Sass S, Mueller NS, Krebs S, Bauersachs S, Kaiser S, Blum H, Thirion C, Krause S, Theis FJ, Pfaffl MW.

PLoS One. 2015 Aug 13;10(8):e0135284. doi: 10.1371/journal.pone.0135284. eCollection 2015.

34.

RDML-Ninja and RDMLdb for standardized exchange of qPCR data.

Ruijter JM, Lefever S, Anckaert J, Hellemans J, Pfaffl MW, Benes V, Bustin SA, Vandesompele J, Untergasser A; RDML consortium.

BMC Bioinformatics. 2015 Jun 20;16:197. doi: 10.1186/s12859-015-0637-6.

35.

Angiogenesis in The Ovary - The Most Important Regulatory Event for Follicle and Corpus Luteum Development and Function in Cow - An Overview.

Berisha B, Schams D, Rodler D, Pfaffl MW.

Anat Histol Embryol. 2016 Apr;45(2):124-30. doi: 10.1111/ahe.12180. Epub 2015 May 6.

PMID:
25951313
36.

Comparison of the miRNome and piRNome of bovine blood and plasma by small RNA sequencing.

Spornraft M, Kirchner B, Pfaffl MW, Riedmaier I.

Biotechnol Lett. 2015 Jun;37(6):1165-76. doi: 10.1007/s10529-015-1788-2. Epub 2015 Feb 21.

PMID:
25700822
37.

TNF-α and IGF1 modify the microRNA signature in skeletal muscle cell differentiation.

Meyer SU, Thirion C, Polesskaya A, Bauersachs S, Kaiser S, Krause S, Pfaffl MW.

Cell Commun Signal. 2015 Jan 29;13:4. doi: 10.1186/s12964-015-0083-0.

38.

Class I odorant receptors, TAS1R and TAS2R taste receptors, are markers for subpopulations of circulating leukocytes.

Malki A, Fiedler J, Fricke K, Ballweg I, Pfaffl MW, Krautwurst D.

J Leukoc Biol. 2015 Mar;97(3):533-45. doi: 10.1189/jlb.2A0714-331RR. Epub 2015 Jan 26.

39.

Optimization of extraction of circulating RNAs from plasma--enabling small RNA sequencing.

Spornraft M, Kirchner B, Haase B, Benes V, Pfaffl MW, Riedmaier I.

PLoS One. 2014 Sep 17;9(9):e107259. doi: 10.1371/journal.pone.0107259. eCollection 2014.

40.

Posttranscriptional regulatory networks: from expression profiling to integrative analysis of mRNA and microRNA data.

Meyer SU, Stoecker K, Sass S, Theis FJ, Pfaffl MW.

Methods Mol Biol. 2014;1160:165-88. doi: 10.1007/978-1-4939-0733-5_15.

PMID:
24740230
41.

mRNA and microRNA purity and integrity: the key to success in expression profiling.

Kirchner B, Paul V, Riedmaier I, Pfaffl MW.

Methods Mol Biol. 2014;1160:43-53. doi: 10.1007/978-1-4939-0733-5_5.

PMID:
24740220
42.

Identification of a potential gene expression biomarker signature in bovine liver to detect the abuse of growth promoters.

Riedmaier I, Spornraft M, Pfaffl MW.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2014 Apr;31(4):641-9. doi: 10.1080/19440049.2014.886341. Epub 2014 Apr 3.

PMID:
24559206
43.

The need for transparency and good practices in the qPCR literature.

Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley G, Wittwer CT, Schjerling P, Day PJ, Abreu M, Aguado B, Beaulieu JF, Beckers A, Bogaert S, Browne JA, Carrasco-Ramiro F, Ceelen L, Ciborowski K, Cornillie P, Coulon S, Cuypers A, De Brouwer S, De Ceuninck L, De Craene J, De Naeyer H, De Spiegelaere W, Deckers K, Dheedene A, Durinck K, Ferreira-Teixeira M, Fieuw A, Gallup JM, Gonzalo-Flores S, Goossens K, Heindryckx F, Herring E, Hoenicka H, Icardi L, Jaggi R, Javad F, Karampelias M, Kibenge F, Kibenge M, Kumps C, Lambertz I, Lammens T, Markey A, Messiaen P, Mets E, Morais S, Mudarra-Rubio A, Nakiwala J, Nelis H, Olsvik PA, Pérez-Novo C, Plusquin M, Remans T, Rihani A, Rodrigues-Santos P, Rondou P, Sanders R, Schmidt-Bleek K, Skovgaard K, Smeets K, Tabera L, Toegel S, Van Acker T, Van den Broeck W, Van der Meulen J, Van Gele M, Van Peer G, Van Poucke M, Van Roy N, Vergult S, Wauman J, Tshuikina-Wiklander M, Willems E, Zaccara S, Zeka F, Vandesompele J.

Nat Methods. 2013 Nov;10(11):1063-7. doi: 10.1038/nmeth.2697.

PMID:
24173381
44.

Nicotinic acetylcholine receptor subunits α4 and α5 associated with smoking behaviour and lung cancer are regulated by upstream open reading frames.

Eggert M, Aichinger E, Pfaffl MW, Steinlein OK, Pfob M.

PLoS One. 2013 Jul 2;8(7):e66157. doi: 10.1371/journal.pone.0066157. Print 2013.

45.

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA.

Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9.

46.

Transcriptional biomarkers.

Pfaffl MW.

Methods. 2013 Jan;59(1):1-2. doi: 10.1016/j.ymeth.2012.12.011. No abstract available.

PMID:
23312615
48.

Effect of non-starch-polysaccharide-degrading enzymes as feed additive on the rumen bacterial population in non-lactating cows quantified by real-time PCR.

Zeitz JO, Guertler P, Pfaffl MW, Eisenreich R, Wiedemann S, Schwarz FJ.

J Anim Physiol Anim Nutr (Berl). 2013 Dec;97(6):1104-13. doi: 10.1111/jpn.12020. Epub 2012 Dec 7.

PMID:
23216628
49.

Evaluation of qPCR curve analysis methods for reliable biomarker discovery: bias, resolution, precision, and implications.

Ruijter JM, Pfaffl MW, Zhao S, Spiess AN, Boggy G, Blom J, Rutledge RG, Sisti D, Lievens A, De Preter K, Derveaux S, Hellemans J, Vandesompele J.

Methods. 2013 Jan;59(1):32-46. doi: 10.1016/j.ymeth.2012.08.011. Epub 2012 Sep 3.

PMID:
22975077
50.

Transcriptional biomarkers--high throughput screening, quantitative verification, and bioinformatical validation methods.

Riedmaier I, Pfaffl MW.

Methods. 2013 Jan;59(1):3-9. doi: 10.1016/j.ymeth.2012.08.012. Epub 2012 Sep 4.

PMID:
22967906

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