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Methods Mol Biol. 2012;835:97-105. doi: 10.1007/978-1-61779-501-5_6.

Identification of HR-inducing cDNAs from plant pathogens via a Gateway(®)-compatible binary Potato virus X-expression vector.

Author information

1
Laboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands. Peter.vanesse@wur.nl

Abstract

Identification of pathogen effectors that elicit a hypersensitive response (HR) in resistant plant hosts is essential to study disease resistance. In this method, it is described how to generate a cDNA library, how to transfer the library into a binary PVX-expression vector, and finally how to set up a high-throughput screen for HR-inducing cDNAs from plant pathogens.

PMID:
22183649
DOI:
10.1007/978-1-61779-501-5_6
[Indexed for MEDLINE]

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