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Items: 44

1.

Probing the role of an invariant active site His in family GH1 β-glycosidases.

Strazzulli A, Perugino G, Mazzone M, Rossi M, Withers SG, Moracci M.

J Enzyme Inhib Med Chem. 2019 Dec;34(1):973-980. doi: 10.1080/14756366.2019.1608198.

2.

The DNA Alkylguanine DNA Alkyltransferase-2 (AGT-2) Of Caenorhabditis Elegans Is Involved In Meiosis And Early Development Under Physiological Conditions.

Serpe M, Forenza C, Adamo A, Russo N, Perugino G, Ciaramella M, Valenti A.

Sci Rep. 2019 May 3;9(1):6889. doi: 10.1038/s41598-019-43394-1.

3.

Thermostability enhancement of the α-carbonic anhydrase from Sulfurihydrogenibium yellowstonense by using the anchoring-and-self-labelling-protein-tag system (ASLtag).

Del Prete S, Merlo R, Valenti A, Mattossovich R, Rossi M, Carginale V, Supuran CT, Perugino G, Capasso C.

J Enzyme Inhib Med Chem. 2019 Dec;34(1):946-954. doi: 10.1080/14756366.2019.1605991.

4.

Optimization of an In Vitro Transcription/Translation System Based on Sulfolobus solfataricus Cell Lysate.

Lo Gullo G, Mattossovich R, Perugino G, La Teana A, Londei P, Benelli D.

Archaea. 2019 Feb 11;2019:9848253. doi: 10.1155/2019/9848253. eCollection 2019.

5.

An AGT-based protein-tag system for the labelling and surface immobilization of enzymes on E. coli outer membrane.

Merlo R, Del Prete S, Valenti A, Mattossovich R, Carginale V, Supuran CT, Capasso C, Perugino G.

J Enzyme Inhib Med Chem. 2019 Dec;34(1):490-499. doi: 10.1080/14756366.2018.1559161.

6.

Crystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with a fluorescent probe.

Rossi F, Morrone C, Massarotti A, Ferraris DM, Valenti A, Perugino G, Miggiano R.

Biochem Biophys Res Commun. 2018 Jun 7;500(3):698-703. doi: 10.1016/j.bbrc.2018.04.139. Epub 2018 Apr 30.

PMID:
29684348
7.

Structure and Properties of DNA Molecules Over The Full Range of Biologically Relevant Supercoiling States.

Bettotti P, Visone V, Lunelli L, Perugino G, Ciaramella M, Valenti A.

Sci Rep. 2018 Apr 18;8(1):6163. doi: 10.1038/s41598-018-24499-5.

8.

Every OGT Is Illuminated … by Fluorescent and Synchrotron Lights.

Miggiano R, Valenti A, Rossi F, Rizzi M, Perugino G, Ciaramella M.

Int J Mol Sci. 2017 Dec 5;18(12). pii: E2613. doi: 10.3390/ijms18122613. Review.

9.

In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag.

Visone V, Han W, Perugino G, Del Monaco G, She Q, Rossi M, Valenti A, Ciaramella M.

PLoS One. 2017 Oct 3;12(10):e0185791. doi: 10.1371/journal.pone.0185791. eCollection 2017.

10.

Interdomain interactions rearrangements control the reaction steps of a thermostable DNA alkyltransferase.

Morrone C, Miggiano R, Serpe M, Massarotti A, Valenti A, Del Monaco G, Rossi M, Rossi F, Rizzi M, Perugino G, Ciaramella M.

Biochim Biophys Acta Gen Subj. 2017 Feb;1861(2):86-96. doi: 10.1016/j.bbagen.2016.10.020. Epub 2016 Oct 22.

PMID:
27777086
11.

Erratum to: A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA-alkyl-transferase by protein engineering.

Vettone A, Serpe M, Hidalgo A, Berenguer J, Del Monaco G, Valenti A, Rossi M, Ciaramella M, Perugino G.

Extremophiles. 2016 Jan;20(1):15-7. doi: 10.1007/s00792-015-0803-9. No abstract available.

PMID:
26666951
12.

Crystal structure of Mycobacterium tuberculosis O6-methylguanine-DNA methyltransferase protein clusters assembled on to damaged DNA.

Miggiano R, Perugino G, Ciaramella M, Serpe M, Rejman D, Páv O, Pohl R, Garavaglia S, Lahiri S, Rizzi M, Rossi F.

Biochem J. 2016 Jan 15;473(2):123-33. doi: 10.1042/BJ20150833. Epub 2015 Oct 28.

PMID:
26512127
13.

A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA- alkyl-transferase by protein engineering.

Vettone A, Serpe M, Hidalgo A, Berenguer J, del Monaco G, Valenti A, Rossi M, Ciaramella M, Perugino G.

Extremophiles. 2016 Jan;20(1):1-13. doi: 10.1007/s00792-015-0791-9. Erratum in: Extremophiles. 2016 Jan;20(1):15-7.

PMID:
26499124
14.

Structure-function relationships governing activity and stability of a DNA alkylation damage repair thermostable protein.

Perugino G, Miggiano R, Serpe M, Vettone A, Valenti A, Lahiri S, Rossi F, Rossi M, Rizzi M, Ciaramella M.

Nucleic Acids Res. 2015 Oct 15;43(18):8801-16. doi: 10.1093/nar/gkv774. Epub 2015 Jul 30.

15.

Chromatin structure and dynamics in hot environments: architectural proteins and DNA topoisomerases of thermophilic archaea.

Visone V, Vettone A, Serpe M, Valenti A, Perugino G, Rossi M, Ciaramella M.

Int J Mol Sci. 2014 Sep 25;15(9):17162-87. doi: 10.3390/ijms150917162. Review.

16.

Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase.

Vettone A, Perugino G, Rossi M, Valenti A, Ciaramella M.

Extremophiles. 2014 Sep;18(5):895-904. doi: 10.1007/s00792-014-0662-9. Epub 2014 Aug 8. Review.

PMID:
25102812
17.

The reverse gyrase from Pyrobaculum calidifontis, a novel extremely thermophilic DNA topoisomerase endowed with DNA unwinding and annealing activities.

Jamroze A, Perugino G, Valenti A, Rashid N, Rossi M, Akhtar M, Ciaramella M.

J Biol Chem. 2014 Feb 7;289(6):3231-43. doi: 10.1074/jbc.M113.517649. Epub 2013 Dec 17.

18.

Biochemical and structural studies of the Mycobacterium tuberculosis O6-methylguanine methyltransferase and mutated variants.

Miggiano R, Casazza V, Garavaglia S, Ciaramella M, Perugino G, Rizzi M, Rossi F.

J Bacteriol. 2013 Jun;195(12):2728-36. doi: 10.1128/JB.02298-12. Epub 2013 Apr 5.

19.

Synergic and opposing activities of thermophilic RecQ-like helicase and topoisomerase 3 proteins in Holliday junction processing and replication fork stabilization.

Valenti A, De Felice M, Perugino G, Bizard A, Nadal M, Rossi M, Ciaramella M.

J Biol Chem. 2012 Aug 31;287(36):30282-95. doi: 10.1074/jbc.M112.366377. Epub 2012 Jun 21.

20.

Thermophilic glycosynthases for oligosaccharides synthesis.

Cobucci-Ponzano B, Perugino G, Strazzulli A, Rossi M, Moracci M.

Methods Enzymol. 2012;510:273-300. doi: 10.1016/B978-0-12-415931-0.00015-X.

PMID:
22608732
21.

Activity and regulation of archaeal DNA alkyltransferase: conserved protein involved in repair of DNA alkylation damage.

Perugino G, Vettone A, Illiano G, Valenti A, Ferrara MC, Rossi M, Ciaramella M.

J Biol Chem. 2012 Feb 3;287(6):4222-31. doi: 10.1074/jbc.M111.308320. Epub 2011 Dec 13. Erratum in: J Biol Chem. 2015 Jan 9;290(2):885.

22.

Positive supercoiling in thermophiles and mesophiles: of the good and evil.

Valenti A, Perugino G, Rossi M, Ciaramella M.

Biochem Soc Trans. 2011 Jan;39(1):58-63. doi: 10.1042/BST0390058. Review.

PMID:
21265747
23.

Engineering the stability and the activity of a glycoside hydrolase.

Cobucci-Ponzano B, Perugino G, Rossi M, Moracci M.

Protein Eng Des Sel. 2011 Jan;24(1-2):21-6. doi: 10.1093/protein/gzq085. Epub 2010 Oct 27. Review.

PMID:
20980336
24.

The archaeal topoisomerase reverse gyrase is a helix-destabilizing protein that unwinds four-way DNA junctions.

Valenti A, Perugino G, Varriale A, D'Auria S, Rossi M, Ciaramella M.

J Biol Chem. 2010 Nov 19;285(47):36532-41. doi: 10.1074/jbc.M110.169029. Epub 2010 Sep 17.

25.

Inhibition of translesion DNA polymerase by archaeal reverse gyrase.

Valenti A, Perugino G, Nohmi T, Rossi M, Ciaramella M.

Nucleic Acids Res. 2009 Jul;37(13):4287-95. doi: 10.1093/nar/gkp386. Epub 2009 May 14.

26.

A new method to assess fetal head descent in labor with transperineal ultrasound.

Barbera AF, Pombar X, Perugino G, Lezotte DC, Hobbins JC.

Ultrasound Obstet Gynecol. 2009 Mar;33(3):313-9. doi: 10.1002/uog.6329.

27.

Reverse gyrase and genome stability in hyperthermophilic organisms.

Perugino G, Valenti A, D'amaro A, Rossi M, Ciaramella M.

Biochem Soc Trans. 2009 Feb;37(Pt 1):69-73. doi: 10.1042/BST0370069. Review.

PMID:
19143604
28.

Dissection of reverse gyrase activities: insight into the evolution of a thermostable molecular machine.

Valenti A, Perugino G, D'Amaro A, Cacace A, Napoli A, Rossi M, Ciaramella M.

Nucleic Acids Res. 2008 Aug;36(14):4587-97. doi: 10.1093/nar/gkn418. Epub 2008 Jul 9.

29.

Isolation and characterization of a new family 42 beta-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: identification of the active site residues.

Di Lauro B, Strazzulli A, Perugino G, La Cara F, Bedini E, Corsaro MM, Rossi M, Moracci M.

Biochim Biophys Acta. 2008 Feb;1784(2):292-301. Epub 2007 Nov 12.

PMID:
18068682
30.

A comparative infrared spectroscopic study of glycoside hydrolases from extremophilic archaea revealed different molecular mechanisms of adaptation to high temperatures.

Ausili A, Cobucci-Ponzano B, Di Lauro B, D'Avino R, Perugino G, Bertoli E, Scirè A, Rossi M, Tanfani F, Moracci M.

Proteins. 2007 Jun 1;67(4):991-1001.

PMID:
17357157
31.

Structural, kinetic, and thermodynamic analysis of glucoimidazole-derived glycosidase inhibitors.

Gloster TM, Roberts S, Perugino G, Rossi M, Moracci M, Panday N, Terinek M, Vasella A, Davies GJ.

Biochemistry. 2006 Oct 3;45(39):11879-84.

PMID:
17002288
32.

Analysis of two polymorphisms in the promoter region of matrix metalloproteinase 1 and 3 genes in women with endometriosis.

Ferrari MM, Biondi ML, Rossi G, Grijuela B, Gaita S, Perugino G, Viganò P.

Acta Obstet Gynecol Scand. 2006;85(2):212-7.

PMID:
16532917
33.

Highly productive autocondensation and transglycosylation reactions with Sulfolobus solfataricus glycosynthase.

Trincone A, Giordano A, Perugino G, Rossi M, Moracci M.

Chembiochem. 2005 Aug;6(8):1431-7.

PMID:
16010696
34.

A novel fatty acid binding protein produced by teratocytes of the aphid parasitoid Aphidius ervi.

Falabella P, Perugino G, Caccialupi P, Riviello L, Varricchio P, Tranfaglia A, Rossi M, Malva C, Graziani F, Moracci M, Pennacchio F.

Insect Mol Biol. 2005 Apr;14(2):195-205.

PMID:
15796753
35.

Structural studies of the beta-glycosidase from Sulfolobus solfataricus in complex with covalently and noncovalently bound inhibitors.

Gloster TM, Roberts S, Ducros VM, Perugino G, Rossi M, Hoos R, Moracci M, Vasella A, Davies GJ.

Biochemistry. 2004 May 25;43(20):6101-9.

PMID:
15147194
36.

Oligosaccharide synthesis by glycosynthases.

Perugino G, Trincone A, Rossi M, Moracci M.

Trends Biotechnol. 2004 Jan;22(1):31-7. Review. No abstract available.

PMID:
14690620
37.

Glycosynthase-catalysed syntheses at pH below neutrality.

Trincone A, Giordano A, Perugino G, Rossi M, Moracci M.

Bioorg Med Chem Lett. 2003 Nov 17;13(22):4039-42.

PMID:
14592503
38.

Activity of hyperthermophilic glycosynthases is significantly enhanced at acidic pH.

Perugino G, Trincone A, Giordano A, van der Oost J, Kaper T, Rossi M, Moracci M.

Biochemistry. 2003 Jul 22;42(28):8484-93.

PMID:
12859194
39.

Enzymatic synthesis of oligosaccharides by two glycosyl hydrolases of Sulfolobus solfataricus.

Moracci M, Trincone A, Cobucci-Ponzano B, Perugino G, Ciaramella M, Rossi M.

Extremophiles. 2001 Jun;5(3):145-52. Review.

PMID:
11453457
40.

Activity and stability of hyperthermophilic enzymes: a comparative study on two archaeal beta-glycosidases.

Pouwels J, Moracci M, Cobucci-Ponzano B, Perugino G, van der Oost J, Kaper T, Lebbink JH, de Vos WM, Ciaramella M, Rossi M.

Extremophiles. 2000 Jun;4(3):157-64.

PMID:
10879560
41.

A novel thermophilic glycosynthase that effects branching glycosylation.

Trincone A, Perugino G, Rossi M, Moracci M.

Bioorg Med Chem Lett. 2000 Feb 21;10(4):365-8.

PMID:
10714501
43.

Association between the activity of the system A amino acid transporter in the microvillous plasma membrane of the human placenta and severity of fetal compromise in intrauterine growth restriction.

Glazier JD, Cetin I, Perugino G, Ronzoni S, Grey AM, Mahendran D, Marconi AM, Pardi G, Sibley CP.

Pediatr Res. 1997 Oct;42(4):514-9.

PMID:
9380446
44.

Maternal concentrations and fetal-maternal concentration differences of plasma amino acids in normal and intrauterine growth-restricted pregnancies.

Cetin I, Ronzoni S, Marconi AM, Perugino G, Corbetta C, Battaglia FC, Pardi G.

Am J Obstet Gynecol. 1996 May;174(5):1575-83.

PMID:
9065132

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