Format

Send to

Choose Destination
J Cell Biol. 2019 Apr 1;218(4):1353-1369. doi: 10.1083/jcb.201808044. Epub 2019 Jan 23.

Coenzyme Q biosynthetic proteins assemble in a substrate-dependent manner into domains at ER-mitochondria contacts.

Author information

1
Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA.
2
Morgridge Institute for Research, Madison, WI.
3
Genome Center of Wisconsin, Madison, WI.
4
Department of Chemistry, University of Wisconsin-Madison, Madison, WI.
5
Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI.
6
Department of Biochemistry, University of Wisconsin-Madison, Madison, WI.
7
Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA jmnunnari@ucdavis.edu.

Abstract

Coenzyme Q (CoQ) lipids are ancient electron carriers that, in eukaryotes, function in the mitochondrial respiratory chain. In mitochondria, CoQ lipids are built by an inner membrane-associated, multicomponent, biosynthetic pathway via successive steps of isoprenyl tail polymerization, 4-hydroxybenzoate head-to-tail attachment, and head modification, resulting in the production of CoQ. In yeast, we discovered that head-modifying CoQ pathway components selectively colocalize to multiple resolvable domains in vivo, representing supramolecular assemblies. In cells engineered with conditional ON or OFF CoQ pathways, domains were strictly correlated with CoQ production and substrate flux, respectively, indicating that CoQ lipid intermediates are required for domain formation. Mitochondrial CoQ domains were also observed in human cells, underscoring their conserved functional importance. CoQ domains within cells were highly enriched adjacent to ER-mitochondria contact sites. Together, our data suggest that CoQ domains function to facilitate substrate accessibility for processive and efficient CoQ production and distribution in cells.

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center