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Items: 1 to 50 of 92

1.

Structure of S-layer protein Sap reveals a mechanism for therapeutic intervention in anthrax.

Fioravanti A, Van Hauwermeiren F, Van der Verren SE, Jonckheere W, Goncalves A, Pardon E, Steyaert J, De Greve H, Lamkanfi M, Remaut H.

Nat Microbiol. 2019 Jul 15. doi: 10.1038/s41564-019-0499-1. [Epub ahead of print]

PMID:
31308522
2.

Domain-interface dynamics of CFTR revealed by stabilizing nanobodies.

Sigoillot M, Overtus M, Grodecka M, Scholl D, Garcia-Pino A, Laeremans T, He L, Pardon E, Hildebrandt E, Urbatsch I, Steyaert J, Riordan JR, Govaerts C.

Nat Commun. 2019 Jun 14;10(1):2636. doi: 10.1038/s41467-019-10714-y.

3.

Interrogating dense ligand chemical space with a forward-synthetic library.

Chevillard F, Stotani S, Karawajczyk A, Hristeva S, Pardon E, Steyaert J, Tzalis D, Kolb P.

Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11496-11501. doi: 10.1073/pnas.1818718116. Epub 2019 May 21.

PMID:
31113876
4.

L amino acid transporter structure and molecular bases for the asymmetry of substrate interaction.

Errasti-Murugarren E, Fort J, Bartoccioni P, Díaz L, Pardon E, Carpena X, Espino-Guarch M, Zorzano A, Ziegler C, Steyaert J, Fernández-Recio J, Fita I, Palacín M.

Nat Commun. 2019 Apr 18;10(1):1807. doi: 10.1038/s41467-019-09837-z.

5.

Structures of ligand-occupied β-Klotho complexes reveal a molecular mechanism underlying endocrine FGF specificity and activity.

Kuzina ES, Ung PM, Mohanty J, Tome F, Choi J, Pardon E, Steyaert J, Lax I, Schlessinger A, Schlessinger J, Lee S.

Proc Natl Acad Sci U S A. 2019 Apr 16;116(16):7819-7824. doi: 10.1073/pnas.1822055116. Epub 2019 Apr 3.

PMID:
30944224
6.

Author Correction: GABAA receptor signalling mechanisms revealed by structural pharmacology.

Masiulis S, Desai R, Uchański T, Martin IS, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR.

Nature. 2019 Feb;566(7744):E8. doi: 10.1038/s41586-019-0929-5.

PMID:
30733619
7.

An improved yeast surface display platform for the screening of nanobody immune libraries.

Uchański T, Zögg T, Yin J, Yuan D, Wohlkönig A, Fischer B, Rosenbaum DM, Kobilka BK, Pardon E, Steyaert J.

Sci Rep. 2019 Jan 23;9(1):382. doi: 10.1038/s41598-018-37212-3.

8.

Structure of Prototypic Peptide Transporter DtpA from E. coli in Complex with Valganciclovir Provides Insights into Drug Binding of Human PepT1.

Ural-Blimke Y, Flayhan A, Strauss J, Rantos V, Bartels K, Nielsen R, Pardon E, Steyaert J, Kosinski J, Quistgaard EM, Löw C.

J Am Chem Soc. 2019 Feb 13;141(6):2404-2412. doi: 10.1021/jacs.8b11343. Epub 2019 Jan 31.

PMID:
30644743
9.

The Molecular Mechanism of Transport by the Mitochondrial ADP/ATP Carrier.

Ruprecht JJ, King MS, Zögg T, Aleksandrova AA, Pardon E, Crichton PG, Steyaert J, Kunji ERS.

Cell. 2019 Jan 24;176(3):435-447.e15. doi: 10.1016/j.cell.2018.11.025. Epub 2019 Jan 2.

10.

GABAA receptor signalling mechanisms revealed by structural pharmacology.

Masiulis S, Desai R, Uchański T, Serna Martin I, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR.

Nature. 2019 Jan;565(7740):454-459. doi: 10.1038/s41586-018-0832-5. Epub 2019 Jan 2. Erratum in: Nature. 2019 Feb 7;:.

11.

Cryo-EM structure of the human α1β3γ2 GABAA receptor in a lipid bilayer.

Laverty D, Desai R, Uchański T, Masiulis S, Stec WJ, Malinauskas T, Zivanov J, Pardon E, Steyaert J, Miller KW, Aricescu AR.

Nature. 2019 Jan;565(7740):516-520. doi: 10.1038/s41586-018-0833-4. Epub 2019 Jan 2.

12.

Publisher Correction: Structural insights into binding specificity, efficacy and bias of a β2AR partial agonist.

Masureel M, Zou Y, Picard LP, van der Westhuizen E, Mahoney JP, Rodrigues JPGLM, Mildorf TJ, Dror RO, Shaw DE, Bouvier M, Pardon E, Steyaert J, Sunahara RK, Weis WI, Zhang C, Kobilka BK.

Nat Chem Biol. 2019 Feb;15(2):205. doi: 10.1038/s41589-018-0182-5.

PMID:
30504785
13.

Challenges in the Structural-Functional Characterization of Multidomain, Partially Disordered Proteins CBP and p300: Preparing Native Proteins and Developing Nanobody Tools.

Bekesi A, Abdellaoui S, Holroyd N, Van Delm W, Pardon E, Pauwels J, Gevaert K, Steyaert J, Derveaux S, Borysik A, Tompa P.

Methods Enzymol. 2018;611:607-675. doi: 10.1016/bs.mie.2018.09.032. Epub 2018 Nov 3.

PMID:
30471702
14.

Characterization and structure determination of a llama-derived nanobody targeting the J-base binding protein 1.

van Beusekom B, Heidebrecht T, Adamopoulos A, Fish A, Pardon E, Steyaert J, Joosten RP, Perrakis A.

Acta Crystallogr F Struct Biol Commun. 2018 Nov 1;74(Pt 11):690-695. doi: 10.1107/S2053230X18010282. Epub 2018 Oct 16.

PMID:
30387773
15.

Structural insights into binding specificity, efficacy and bias of a β2AR partial agonist.

Masureel M, Zou Y, Picard LP, van der Westhuizen E, Mahoney JP, Rodrigues JPGLM, Mildorf TJ, Dror RO, Shaw DE, Bouvier M, Pardon E, Steyaert J, Sunahara RK, Weis WI, Zhang C, Kobilka BK.

Nat Chem Biol. 2018 Nov;14(11):1059-1066. doi: 10.1038/s41589-018-0145-x. Epub 2018 Oct 16. Erratum in: Nat Chem Biol. 2019 Feb;15(2):205.

16.

Crystal structure of human Mediator subunit MED23.

Monté D, Clantin B, Dewitte F, Lens Z, Rucktooa P, Pardon E, Steyaert J, Verger A, Villeret V.

Nat Commun. 2018 Aug 23;9(1):3389. doi: 10.1038/s41467-018-05967-y.

17.

Crystal Structure of a ligand-bound LacY-Nanobody Complex.

Kumar H, Finer-Moore JS, Jiang X, Smirnova I, Kasho V, Pardon E, Steyaert J, Kaback HR, Stroud RM.

Proc Natl Acad Sci U S A. 2018 Aug 28;115(35):8769-8774. doi: 10.1073/pnas.1801774115. Epub 2018 Aug 14.

18.

Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor.

Gulati S, Jin H, Masuho I, Orban T, Cai Y, Pardon E, Martemyanov KA, Kiser PD, Stewart PL, Ford CP, Steyaert J, Palczewski K.

Nat Commun. 2018 May 18;9(1):1996. doi: 10.1038/s41467-018-04432-0.

19.

Binding Specificities of Nanobody•Membrane Protein Complexes Obtained from Chemical Cross-Linking and High-Mass MALDI Mass Spectrometry.

Köhler M, Neff C, Perez C, Brunner C, Pardon E, Steyaert J, Schneider G, Locher KP, Zenobi R.

Anal Chem. 2018 Apr 17;90(8):5306-5313. doi: 10.1021/acs.analchem.8b00236. Epub 2018 Apr 4.

PMID:
29562137
20.

Nanobody-Enabled Reverse Pharmacology on G-Protein-Coupled Receptors.

Pardon E, Betti C, Laeremans T, Chevillard F, Guillemyn K, Kolb P, Ballet S, Steyaert J.

Angew Chem Int Ed Engl. 2018 May 4;57(19):5292-5295. doi: 10.1002/anie.201712581. Epub 2018 Mar 30.

PMID:
29469969
21.

Binding-Site Compatible Fragment Growing Applied to the Design of β2-Adrenergic Receptor Ligands.

Chevillard F, Rimmer H, Betti C, Pardon E, Ballet S, van Hilten N, Steyaert J, Diederich WE, Kolb P.

J Med Chem. 2018 Feb 8;61(3):1118-1129. doi: 10.1021/acs.jmedchem.7b01558. Epub 2018 Jan 24.

PMID:
29364664
22.

Structures of β-klotho reveal a 'zip code'-like mechanism for endocrine FGF signalling.

Lee S, Choi J, Mohanty J, Sousa LP, Tome F, Pardon E, Steyaert J, Lemmon MA, Lax I, Schlessinger J.

Nature. 2018 Jan 25;553(7689):501-505. doi: 10.1038/nature25010. Epub 2018 Jan 17.

23.

Structure of the Nanobody-Stabilized Active State of the Kappa Opioid Receptor.

Che T, Majumdar S, Zaidi SA, Ondachi P, McCorvy JD, Wang S, Mosier PD, Uprety R, Vardy E, Krumm BE, Han GW, Lee MY, Pardon E, Steyaert J, Huang XP, Strachan RT, Tribo AR, Pasternak GW, Carroll FI, Stevens RC, Cherezov V, Katritch V, Wacker D, Roth BL.

Cell. 2018 Jan 11;172(1-2):55-67.e15. doi: 10.1016/j.cell.2017.12.011. Epub 2018 Jan 4.

24.

Structure of PINK1 in complex with its substrate ubiquitin.

Schubert AF, Gladkova C, Pardon E, Wagstaff JL, Freund SMV, Steyaert J, Maslen SL, Komander D.

Nature. 2017 Dec 7;552(7683):51-56. doi: 10.1038/nature24645. Epub 2017 Oct 30.

25.

Structural basis for GABAA receptor potentiation by neurosteroids.

Miller PS, Scott S, Masiulis S, De Colibus L, Pardon E, Steyaert J, Aricescu AR.

Nat Struct Mol Biol. 2017 Nov;24(11):986-992. doi: 10.1038/nsmb.3484. Epub 2017 Oct 9.

26.

Generation and Characterization of Anti-VGLUT Nanobodies Acting as Inhibitors of Transport.

Schenck S, Kunz L, Sahlender D, Pardon E, Geertsma ER, Savtchouk I, Suzuki T, Neldner Y, Štefanić S, Steyaert J, Volterra A, Dutzler R.

Biochemistry. 2017 Aug 1;56(30):3962-3971. doi: 10.1021/acs.biochem.7b00436. Epub 2017 Jul 21.

PMID:
28731329
27.

Crystallographic and biochemical characterization of the dimeric architecture of site-2 protease.

Schacherl M, Gompert M, Pardon E, Lamkemeyer T, Steyaert J, Baumann U.

Biochim Biophys Acta Biomembr. 2017 Oct;1859(10):1859-1871. doi: 10.1016/j.bbamem.2017.05.006. Epub 2017 May 11.

28.

Rational Design of Nanobody80 Loop Peptidomimetics: Towards Biased β2 Adrenergic Receptor Ligands.

Martin C, Moors SLC, Danielsen M, Betti C, Fabris C, Sejer Pedersen D, Pardon E, Peyressatre M, Fehér K, Martins JC, Mosolff Mathiesen J, Morris MC, Devoogdt N, Caveliers V, De Proft F, Steyaert J, Ballet S.

Chemistry. 2017 Jul 18;23(40):9632-9640. doi: 10.1002/chem.201701321. Epub 2017 Jun 29.

PMID:
28449310
29.

A specific nanobody prevents amyloidogenesis of D76N β2-microglobulin in vitro and modifies its tissue distribution in vivo.

Raimondi S, Porcari R, Mangione PP, Verona G, Marcoux J, Giorgetti S, Taylor GW, Ellmerich S, Ballico M, Zanini S, Pardon E, Al-Shawi R, Simons JP, Corazza A, Fogolari F, Leri M, Stefani M, Bucciantini M, Gillmore JD, Hawkins PN, Valli M, Stoppini M, Robinson CV, Steyaert J, Esposito G, Bellotti V.

Sci Rep. 2017 Apr 21;7:46711. doi: 10.1038/srep46711.

30.

Leishmania donovani tyrosyl-tRNA synthetase structure in complex with a tyrosyl adenylate analog and comparisons with human and protozoan counterparts.

Barros-Álvarez X, Kerchner KM, Koh CY, Turley S, Pardon E, Steyaert J, Ranade RM, Gillespie JR, Zhang Z, Verlinde CLMJ, Fan E, Buckner FS, Hol WGJ.

Biochimie. 2017 Jul;138:124-136. doi: 10.1016/j.biochi.2017.04.006. Epub 2017 Apr 18.

31.

Structural basis of inhibition of lipid-linked oligosaccharide flippase PglK by a conformational nanobody.

Perez C, Köhler M, Janser D, Pardon E, Steyaert J, Zenobi R, Locher KP.

Sci Rep. 2017 Apr 19;7:46641. doi: 10.1038/srep46641.

32.

Crystal structure of a LacY-nanobody complex in a periplasmic-open conformation.

Jiang X, Smirnova I, Kasho V, Wu J, Hirata K, Ke M, Pardon E, Steyaert J, Yan N, Kaback HR.

Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12420-12425. Epub 2016 Oct 19.

33.

Thermodynamics of Nanobody Binding to Lactose Permease.

Hariharan P, Andersson M, Jiang X, Pardon E, Steyaert J, Kaback HR, Guan L.

Biochemistry. 2016 Oct 25;55(42):5917-5926. doi: 10.1021/acs.biochem.6b00826. Epub 2016 Oct 12.

34.

Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation.

Staus DP, Strachan RT, Manglik A, Pani B, Kahsai AW, Kim TH, Wingler LM, Ahn S, Chatterjee A, Masoudi A, Kruse AC, Pardon E, Steyaert J, Weis WI, Prosser RS, Kobilka BK, Costa T, Lefkowitz RJ.

Nature. 2016 Jul 21;535(7612):448-52. Epub 2016 Jul 13.

35.

Allosteric coupling from G protein to the agonist-binding pocket in GPCRs.

DeVree BT, Mahoney JP, Vélez-Ruiz GA, Rasmussen SG, Kuszak AJ, Edwald E, Fung JJ, Manglik A, Masureel M, Du Y, Matt RA, Pardon E, Steyaert J, Kobilka BK, Sunahara RK.

Nature. 2016 Jul 7;535(7610):182-6. Epub 2016 Jun 29.

36.

The unexpected structure of the designed protein Octarellin V.1 forms a challenge for protein structure prediction tools.

Figueroa M, Sleutel M, Vandevenne M, Parvizi G, Attout S, Jacquin O, Vandenameele J, Fischer AW, Damblon C, Goormaghtigh E, Valerio-Lepiniec M, Urvoas A, Durand D, Pardon E, Steyaert J, Minard P, Maes D, Meiler J, Matagne A, Martial JA, Van de Weerdt C.

J Struct Biol. 2016 Jul;195(1):19-30. doi: 10.1016/j.jsb.2016.05.004. Epub 2016 May 12.

37.

Corrigendum: Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family.

Geertsma ER, Chang YN, Shaik FR, Neldner Y, Pardon E, Steyaert J, Dutzler R.

Nat Struct Mol Biol. 2016 May 4;23(5):462. doi: 10.1038/nsmb0516-462. No abstract available.

PMID:
27142325
38.

Functional and Biochemical Characterization of Alvinella pompejana Cys-Loop Receptor Homologues.

Wijckmans E, Nys M, Debaveye S, Brams M, Pardon E, Willegems K, Bertrand D, Steyaert J, Efremov R, Ulens C.

PLoS One. 2016 Mar 21;11(3):e0151183. doi: 10.1371/journal.pone.0151183. eCollection 2016.

39.

Transient conformers of LacY are trapped by nanobodies.

Smirnova I, Kasho V, Jiang X, Pardon E, Steyaert J, Kaback HR.

Proc Natl Acad Sci U S A. 2015 Nov 10;112(45):13839-44. doi: 10.1073/pnas.1519485112. Epub 2015 Oct 28. Erratum in: Proc Natl Acad Sci U S A. 2016 Jan 5;113(1):E101.

40.

Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes.

Rostislavleva K, Soler N, Ohashi Y, Zhang L, Pardon E, Burke JE, Masson GR, Johnson C, Steyaert J, Ktistakis NT, Williams RL.

Science. 2015 Oct 9;350(6257):aac7365. doi: 10.1126/science.aac7365.

41.

Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family.

Geertsma ER, Chang YN, Shaik FR, Neldner Y, Pardon E, Steyaert J, Dutzler R.

Nat Struct Mol Biol. 2015 Oct;22(10):803-8. doi: 10.1038/nsmb.3091. Epub 2015 Sep 14. Erratum in: Nat Struct Mol Biol. 2016 May 4;23 (5):462.

PMID:
26367249
42.

Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.

Newman JA, Savitsky P, Allerston CK, Bizard AH, Özer Ö, Sarlós K, Liu Y, Pardon E, Steyaert J, Hickson ID, Gileadi O.

Nucleic Acids Res. 2015 May 26;43(10):5221-35. doi: 10.1093/nar/gkv373. Epub 2015 Apr 21.

43.

Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies.

Moonens K, Van den Broeck I, Okello E, Pardon E, De Kerpel M, Remaut H, De Greve H.

Vet Res. 2015 Feb 24;46:14. doi: 10.1186/s13567-015-0151-x.

44.

Outward-facing conformers of LacY stabilized by nanobodies.

Smirnova I, Kasho V, Jiang X, Pardon E, Steyaert J, Kaback HR.

Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):18548-53. doi: 10.1073/pnas.1422265112. Epub 2014 Dec 15.

45.

Nanobody mediated inhibition of attachment of F18 Fimbriae expressing Escherichia coli.

Moonens K, De Kerpel M, Coddens A, Cox E, Pardon E, Remaut H, De Greve H.

PLoS One. 2014 Dec 11;9(12):e114691. doi: 10.1371/journal.pone.0114691. eCollection 2014.

46.

Llama immunization with full-length VAR2CSA generates cross-reactive and inhibitory single-domain antibodies against the DBL1X domain.

Nunes-Silva S, Gangnard S, Vidal M, Vuchelen A, Dechavanne S, Chan S, Pardon E, Steyaert J, Ramboarina S, Chêne A, Gamain B.

Sci Rep. 2014 Dec 9;4:7373. doi: 10.1038/srep07373.

47.

Production, crystallization and preliminary X-ray diffraction of the Gαs α-helical domain in complex with a nanobody.

Triest S, Wohlkönig A, Pardon E, Steyaert J.

Acta Crystallogr F Struct Biol Commun. 2014 Nov;70(Pt 11):1504-7. doi: 10.1107/S2053230X14020962. Epub 2014 Oct 25.

48.

Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport.

Ehrnstorfer IA, Geertsma ER, Pardon E, Steyaert J, Dutzler R.

Nat Struct Mol Biol. 2014 Nov;21(11):990-6. doi: 10.1038/nsmb.2904. Epub 2014 Oct 19.

PMID:
25326704
49.

The molecular mechanism of Shiga toxin Stx2e neutralization by a single-domain antibody targeting the cell receptor-binding domain.

Lo AW, Moonens K, De Kerpel M, Brys L, Pardon E, Remaut H, De Greve H.

J Biol Chem. 2014 Sep 5;289(36):25374-81. doi: 10.1074/jbc.M114.566257. Epub 2014 Jul 22.

50.

SAXS analysis of the tRNA-modifying enzyme complex MnmE/MnmG reveals a novel interaction mode and GTP-induced oligomerization.

Fislage M, Brosens E, Deyaert E, Spilotros A, Pardon E, Loris R, Steyaert J, Garcia-Pino A, Versées W.

Nucleic Acids Res. 2014 May;42(9):5978-92. doi: 10.1093/nar/gku213. Epub 2014 Mar 14.

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