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Items: 49


Strong gene activation in plants with genome-wide specificity using a new orthogonal CRISPR/Cas9-based programmable transcriptional activator.

Selma S, Bernabé-Orts JM, Vazquez-Vilar M, Diego-Martin B, Ajenjo M, Garcia-Carpintero V, Granell A, Orzaez D.

Plant Biotechnol J. 2019 Sep;17(9):1703-1705. doi: 10.1111/pbi.13138. Epub 2019 May 23. No abstract available.


Assessment of Cas12a-mediated gene editing efficiency in plants.

Bernabé-Orts JM, Casas-Rodrigo I, Minguet EG, Landolfi V, Garcia-Carpintero V, Gianoglio S, Vázquez-Vilar M, Granell A, Orzaez D.

Plant Biotechnol J. 2019 Oct;17(10):1971-1984. doi: 10.1111/pbi.13113. Epub 2019 Apr 19.


DNA assembly standards: Setting the low-level programming code for plant biotechnology.

Vazquez-Vilar M, Orzaez D, Patron N.

Plant Sci. 2018 Aug;273:33-41. doi: 10.1016/j.plantsci.2018.02.024. Epub 2018 Mar 3. Review.


FungalBraid: A GoldenBraid-based modular cloning platform for the assembly and exchange of DNA elements tailored to fungal synthetic biology.

Hernanz-Koers M, Gandía M, Garrigues S, Manzanares P, Yenush L, Orzaez D, Marcos JF.

Fungal Genet Biol. 2018 Jul;116:51-61. doi: 10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20.


Evolutionarily distinct carotenoid cleavage dioxygenases are responsible for crocetin production in Buddleja davidii.

Ahrazem O, Diretto G, Argandoña J, Rubio-Moraga Á, Julve JM, Orzáez D, Granell A, Gómez-Gómez L.

J Exp Bot. 2017 Jul 20;68(16):4663-4677. doi: 10.1093/jxb/erx277.


A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins.

Julve Parreño JM, Huet E, Fernández-Del-Carmen A, Segura A, Venturi M, Gandía A, Pan WS, Albaladejo I, Forment J, Pla D, Wigdorovitz A, Calvete JJ, Gutiérrez C, Gutiérrez JM, Granell A, Orzáez D.

Plant Biotechnol J. 2018 Mar;16(3):727-736. doi: 10.1111/pbi.12823. Epub 2017 Sep 22.


Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins.

Pasin F, Bedoya LC, Bernabé-Orts JM, Gallo A, Simón-Mateo C, Orzaez D, García JA.

ACS Synth Biol. 2017 Oct 20;6(10):1962-1968. doi: 10.1021/acssynbio.6b00354. Epub 2017 Jun 28.


Uncovering tomato quantitative trait loci and candidate genes for fruit cuticular lipid composition using the Solanum pennellii introgression line population.

Fernandez-Moreno JP, Levy-Samoha D, Malitsky S, Monforte AJ, Orzaez D, Aharoni A, Granell A.

J Exp Bot. 2017 May 17;68(11):2703-2716. doi: 10.1093/jxb/erx134.


GB3.0: a platform for plant bio-design that connects functional DNA elements with associated biological data.

Vazquez-Vilar M, Quijano-Rubio A, Fernandez-Del-Carmen A, Sarrion-Perdigones A, Ochoa-Fernandez R, Ziarsolo P, Blanca J, Granell A, Orzaez D.

Nucleic Acids Res. 2017 Feb 28;45(4):2196-2209. doi: 10.1093/nar/gkw1326.


Characterization of a New Pink-Fruited Tomato Mutant Results in the Identification of a Null Allele of the SlMYB12 Transcription Factor.

Fernandez-Moreno JP, Tzfadia O, Forment J, Presa S, Rogachev I, Meir S, Orzaez D, Aharoni A, Granell A.

Plant Physiol. 2016 Jul;171(3):1821-36. doi: 10.1104/pp.16.00282. Epub 2016 May 6.


Biomanufacturing of protective antibodies and other therapeutics in edible plant tissues for oral applications.

Juarez P, Virdi V, Depicker A, Orzaez D.

Plant Biotechnol J. 2016 Sep;14(9):1791-9. doi: 10.1111/pbi.12541. Epub 2016 Feb 13. Review.


Transient Glyco-Engineering to Produce Recombinant IgA1 with Defined N- and O-Glycans in Plants.

Dicker M, Tschofen M, Maresch D, König J, Juarez P, Orzaez D, Altmann F, Steinkellner H, Strasser R.

Front Plant Sci. 2016 Jan 29;7:18. doi: 10.3389/fpls.2016.00018. eCollection 2016.


A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.

Vazquez-Vilar M, Bernabé-Orts JM, Fernandez-Del-Carmen A, Ziarsolo P, Blanca J, Granell A, Orzaez D.

Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.


Elucidation of the first committed step in betalain biosynthesis enables the heterologous engineering of betalain pigments in plants.

Polturak G, Breitel D, Grossman N, Sarrion-Perdigones A, Weithorn E, Pliner M, Orzaez D, Granell A, Rogachev I, Aharoni A.

New Phytol. 2016 Apr;210(1):269-83. doi: 10.1111/nph.13796. Epub 2015 Dec 18.


Standards for plant synthetic biology: a common syntax for exchange of DNA parts.

Patron NJ, Orzaez D, Marillonnet S, Warzecha H, Matthewman C, Youles M, Raitskin O, Leveau A, Farré G, Rogers C, Smith A, Hibberd J, Webb AA, Locke J, Schornack S, Ajioka J, Baulcombe DC, Zipfel C, Kamoun S, Jones JD, Kuhn H, Robatzek S, Van Esse HP, Sanders D, Oldroyd G, Martin C, Field R, O'Connor S, Fox S, Wulff B, Miller B, Breakspear A, Radhakrishnan G, Delaux PM, Loqué D, Granell A, Tissier A, Shih P, Brutnell TP, Quick WP, Rischer H, Fraser PD, Aharoni A, Raines C, South PF, Ané JM, Hamberger BR, Langdale J, Stougaard J, Bouwmeester H, Udvardi M, Murray JA, Ntoukakis V, Schäfer P, Denby K, Edwards KJ, Osbourn A, Haseloff J.

New Phytol. 2015 Oct;208(1):13-9. doi: 10.1111/nph.13532. Epub 2015 Jul 14. Erratum in: New Phytol. 2016 Jan;209(2):885.


Software-assisted stacking of gene modules using GoldenBraid 2.0 DNA-assembly framework.

Vazquez-Vilar M, Sarrion-Perdigones A, Ziarsolo P, Blanca J, Granell A, Orzaez D.

Methods Mol Biol. 2015;1284:399-420. doi: 10.1007/978-1-4939-2444-8_20.


New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus.

Rubio-Moraga A, Rambla JL, Fernández-de-Carmen A, Trapero-Mozos A, Ahrazem O, Orzáez D, Granell A, Gómez-Gómez L.

Plant Mol Biol. 2014 Nov;86(4-5):555-69. doi: 10.1007/s11103-014-0250-5. Epub 2014 Sep 10.


Evaluation of unintended effects in the composition of tomatoes expressing a human immunoglobulin A against rotavirus.

Juarez P, Fernandez-del-Carmen A, Rambla JL, Presa S, Mico A, Granell A, Orzaez D.

J Agric Food Chem. 2014 Aug 13;62(32):8158-68. doi: 10.1021/jf502292g. Epub 2014 Aug 1.


Increasing tomato fruit quality by enhancing fruit chloroplast function. A double-edged sword?

Cocaliadis MF, Fernández-Muñoz R, Pons C, Orzaez D, Granell A.

J Exp Bot. 2014 Aug;65(16):4589-98. doi: 10.1093/jxb/eru165. Epub 2014 Apr 10. Review.


Design and construction of multigenic constructs for plant biotechnology using the GoldenBraid cloning strategy.

Sarrion-Perdigones A, Palaci J, Granell A, Orzaez D.

Methods Mol Biol. 2014;1116:133-51. doi: 10.1007/978-1-62703-764-8_10.


Anthocyanins double the shelf life of tomatoes by delaying overripening and reducing susceptibility to gray mold.

Zhang Y, Butelli E, De Stefano R, Schoonbeek HJ, Magusin A, Pagliarani C, Wellner N, Hill L, Orzaez D, Granell A, Jones JD, Martin C.

Curr Biol. 2013 Jun 17;23(12):1094-100. doi: 10.1016/j.cub.2013.04.072. Epub 2013 May 23.


GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology.

Sarrion-Perdigones A, Vazquez-Vilar M, Palací J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D.

Plant Physiol. 2013 Jul;162(3):1618-31.


A VIN1 GUS::GFP fusion reveals activated sucrose metabolism programming occurring in interspersed cells during tomato fruit ripening.

Estornell LH, Pons C, Martínez A, O'Connor JE, Orzaez D, Granell A.

J Plant Physiol. 2013 Aug 15;170(12):1113-21. doi: 10.1016/j.jplph.2013.03.007. Epub 2013 Apr 15.


Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants.

Juarez P, Huet-Trujillo E, Sarrion-Perdigones A, Falconi EE, Granell A, Orzaez D.

Int J Mol Sci. 2013 Mar 18;14(3):6205-22. doi: 10.3390/ijms14036205.


A coat-independent superinfection exclusion rapidly imposed in Nicotiana benthamiana cells by tobacco mosaic virus is not prevented by depletion of the movement protein.

Julve JM, Gandía A, Fernández-Del-Carmen A, Sarrion-Perdigones A, Castelijns B, Granell A, Orzaez D.

Plant Mol Biol. 2013 Apr;81(6):553-64. doi: 10.1007/s11103-013-0028-1. Epub 2013 Feb 17.


VIGS: a tool to study fruit development in Solanum lycopersicum.

Fernandez-Moreno JP, Orzaez D, Granell A.

Methods Mol Biol. 2013;975:183-96. doi: 10.1007/978-1-62703-278-0_14.


Funding decisions: Romania needs overseas reviewers.

Altosaar I, Oehmen A, Almeida CM, De Pascale S, de Tommasi N, Domínguez de María P, Orzaez D, Zervos A, Xu C.

Nature. 2012 Dec 13;492(7428):186. doi: 10.1038/492186c. No abstract available.


Recombinant jacalin-like plant lectins are produced at high levels in Nicotiana benthamiana and retain agglutination activity and sugar specificity.

Fernandez-del-Carmen A, Juárez P, Presa S, Granell A, Orzáez D.

J Biotechnol. 2013 Feb 20;163(4):391-400. doi: 10.1016/j.jbiotec.2012.11.017. Epub 2012 Dec 7.


Exploiting Multisite Gateway and pENFRUIT plasmid collection for fruit genetic engineering.

Estornell LH, Granell A, Orzaez D.

Methods Mol Biol. 2012;847:351-68. doi: 10.1007/978-1-61779-558-9_28.


Visual tracking of plant virus infection and movement using a reporter MYB transcription factor that activates anthocyanin biosynthesis.

Bedoya LC, Martínez F, Orzáez D, Daròs JA.

Plant Physiol. 2012 Mar;158(3):1130-8. doi: 10.1104/pp.111.192922. Epub 2012 Jan 11.


Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes.

Juárez P, Presa S, Espí J, Pineda B, Antón MT, Moreno V, Buesa J, Granell A, Orzaez D.

Plant Biotechnol J. 2012 Apr;10(3):341-52. doi: 10.1111/j.1467-7652.2011.00666.x. Epub 2011 Nov 10.


GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.

Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, Granell A, Orzaez D.

PLoS One. 2011;6(7):e21622. doi: 10.1371/journal.pone.0021622. Epub 2011 Jul 7.


In planta production of plant-derived and non-plant-derived adjuvants.

Granell A, Fernández del-Carmen A, Orzáez D.

Expert Rev Vaccines. 2010 Aug;9(8):843-58. doi: 10.1586/erv.10.80. Review.


Using genetic variability available in the breeder's pool to engineer fruit quality.

Orzaez D, Monforte AJ, Granell A.

GM Crops. 2010 May-Jun;1(3):120-7. doi: 10.4161/gmcr.1.3.12327. Review.


Manufacturing antibodies in the plant cell.

Orzáez D, Granell A, Blázquez MA.

Biotechnol J. 2009 Dec;4(12):1712-24. doi: 10.1002/biot.200900223. Review.


Biochemical and molecular analysis of pink tomatoes: deregulated expression of the gene encoding transcription factor SlMYB12 leads to pink tomato fruit color.

Ballester AR, Molthoff J, de Vos R, Hekkert Bt, Orzaez D, Fernández-Moreno JP, Tripodi P, Grandillo S, Martin C, Heldens J, Ykema M, Granell A, Bovy A.

Plant Physiol. 2010 Jan;152(1):71-84. doi: 10.1104/pp.109.147322. Epub 2009 Nov 11.


Reverse genetics and transient gene expression in fleshy fruits: overcoming plant stable transformation.

Orzaez D, Granell A.

Plant Signal Behav. 2009 Sep;4(9):864-7. Epub 2009 Sep 29.


A visual reporter system for virus-induced gene silencing in tomato fruit based on anthocyanin accumulation.

Orzaez D, Medina A, Torre S, Fernández-Moreno JP, Rambla JL, Fernández-Del-Carmen A, Butelli E, Martin C, Granell A.

Plant Physiol. 2009 Jul;150(3):1122-34. doi: 10.1104/pp.109.139006. Epub 2009 May 8.


A multisite gateway-based toolkit for targeted gene expression and hairpin RNA silencing in tomato fruits.

Estornell LH, Orzáez D, López-Peña L, Pineda B, Antón MT, Moreno V, Granell A.

Plant Biotechnol J. 2009 Apr;7(3):298-309. doi: 10.1111/j.1467-7652.2009.00402.x. Epub 2009 Feb 17.


Cytosolic and plastoglobule-targeted carotenoid dioxygenases from Crocus sativus are both involved in beta-ionone release.

Rubio A, Rambla JL, Santaella M, Gómez MD, Orzaez D, Granell A, Gómez-Gómez L.

J Biol Chem. 2008 Sep 5;283(36):24816-25. doi: 10.1074/jbc.M804000200. Epub 2008 Jul 7.


Silencing of DELLA induces facultative parthenocarpy in tomato fruits.

Martí C, Orzáez D, Ellul P, Moreno V, Carbonell J, Granell A.

Plant J. 2007 Dec;52(5):865-76. Epub 2007 Sep 19.


Plant expression of chicken secretory antibodies derived from combinatorial libraries.

Wieland WH, Lammers A, Schots A, Orzáez DV.

J Biotechnol. 2006 Apr 10;122(3):382-91. Epub 2006 Jan 31.


Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit.

Orzaez D, Mirabel S, Wieland WH, Granell A.

Plant Physiol. 2006 Jan;140(1):3-11.


Display and selection of chicken IgA Fab fragments.

Wieland WH, Orzáez D, Lammers A, Parmentier HK, Schots A.

Vet Immunol Immunopathol. 2006 Mar 15;110(1-2):129-40. Epub 2005 Nov 8.


A functional polymeric immunoglobulin receptor in chicken (Gallus gallus) indicates ancient role of secretory IgA in mucosal immunity.

Wieland WH, Orzáez D, Lammers A, Parmentier HK, Verstegen MW, Schots A.

Biochem J. 2004 Jun 15;380(Pt 3):669-76.


A tomato homologue of the human protein PIRIN is induced during programmed cell death.

Orzaez D, de Jong AJ, Woltering EJ.

Plant Mol Biol. 2001 Jul;46(4):459-68.


Changes in gene expression during programmed cell death in tomato cell suspensions.

Hoeberichts FA, Orzaez D, van der Plas LH, Woltering EJ.

Plant Mol Biol. 2001 Apr;45(6):641-54.


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