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Science. 2018 Dec 21;362(6421):1407-1410. doi: 10.1126/science.aau3956.

Root branching toward water involves posttranslational modification of transcription factor ARF7.

Author information

1
Department of Biosciences, University of Durham, Durham DH1 3LE, UK.
2
Plant and Crop Sciences, School of Biosciences, University of Nottingham, Sutton Bonington LE12 5RD, UK.
3
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, F-69342, Lyon, France.
4
Department of Biology, Graduate School of Science, Kobe University, Kobe 657-8501, Japan.
5
Department of Plant and Microbial Biology, University of Zurich, CH-8008 Zurich, Switzerland.
6
Developmental Biology, Wageningen University and Research, Wageningen, Netherlands.
7
Department of Biology, Stanford University, Stanford, CA 94305, USA.
8
School of Computer Science, Jubilee Campus, University of Nottingham, Nottingham NG8 1BB, UK.
9
Department of Biosciences, University of Durham, Durham DH1 3LE, UK. ari.sadanandom@durham.ac.uk malcolm.bennett@nottingham.ac.uk.
10
Plant and Crop Sciences, School of Biosciences, University of Nottingham, Sutton Bonington LE12 5RD, UK. ari.sadanandom@durham.ac.uk malcolm.bennett@nottingham.ac.uk.

Abstract

Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water by using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. This transcription factor induces asymmetric expression of its target gene LBD16 in lateral root founder cells. This differential expression pattern is regulated by posttranslational modification of ARF7 with the small ubiquitin-like modifier (SUMO) protein. SUMOylation negatively regulates ARF7 DNA binding activity. ARF7 SUMOylation is required to recruit the Aux/IAA (indole-3-acetic acid) repressor protein IAA3. Blocking ARF7 SUMOylation disrupts IAA3 recruitment and hydropatterning. We conclude that SUMO-dependent regulation of auxin response controls root branching pattern in response to water availability.

PMID:
30573626
DOI:
10.1126/science.aau3956
[Indexed for MEDLINE]

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