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Items: 1 to 50 of 69

1.

Comprehensive analysis of metabolic sensitivity of 1,4-butanediol producing E. coli towards substrate and oxygen availability.

Pooth V, van Gaalen K, Trenkamp S, Wiechert W, Oldiges M.

Biotechnol Prog. 2019 Oct 6:e2917. doi: 10.1002/btpr.2917. [Epub ahead of print]

PMID:
31587523
2.

A FRET-based biosensor for the quantification of glucose in culture supernatants of mL scale microbial cultivations.

Otten J, Tenhaef N, Jansen RP, Döbber J, Jungbluth L, Noack S, Oldiges M, Wiechert W, Pohl M.

Microb Cell Fact. 2019 Aug 21;18(1):143. doi: 10.1186/s12934-019-1193-y.

3.

FeedER: a feedback-regulated enzyme-based slow-release system for fed-batch cultivation in microtiter plates.

Jansen R, Tenhaef N, Moch M, Wiechert W, Noack S, Oldiges M.

Bioprocess Biosyst Eng. 2019 Nov;42(11):1843-1852. doi: 10.1007/s00449-019-02180-z. Epub 2019 Aug 9.

PMID:
31399865
4.

A closer look at Aspergillus: online monitoring via scattered light enables reproducible phenotyping.

Jansen RP, Beuck C, Moch M, Klein B, Küsters K, Morschett H, Wiechert W, Oldiges M.

Fungal Biol Biotechnol. 2019 Aug 5;6:11. doi: 10.1186/s40694-019-0073-x. eCollection 2019.

5.

Direct Analysis of Underivatized Amino Acids in Plant Extracts by LC-MS/MS (Improved Method).

Thiele B, Hupert M, Santiago-Schübel B, Oldiges M, Hofmann D.

Methods Mol Biol. 2019;2030:403-414. doi: 10.1007/978-1-4939-9639-1_30.

PMID:
31347134
6.

Carboxylic acid consumption and production by Corynebacterium glutamicum.

Conrady M, Lemoine A, Limberg MH, Oldiges M, Neubauer P, Junne S.

Biotechnol Prog. 2019 May;35(3):e2804. doi: 10.1002/btpr.2804. Epub 2019 Mar 23.

PMID:
30851150
7.

Characterization of Sigma Factor Genes in Streptomyces lividans TK24 Using a Genomic Library-Based Approach for Multiple Gene Deletions.

Rebets Y, Tsolis KC, Guðmundsdóttir EE, Koepff J, Wawiernia B, Busche T, Bleidt A, Horbal L, Myronovskyi M, Ahmed Y, Wiechert W, Rückert C, Hamed MB, Bilyk B, Anné J, Friðjónsson Ó, Kalinowski J, Oldiges M, Economou A, Luzhetskyy A.

Front Microbiol. 2018 Dec 10;9:3033. doi: 10.3389/fmicb.2018.03033. eCollection 2018.

8.

Monitoring Protein Secretion in Streptomyces Using Fluorescent Proteins.

Hamed MB, Vrancken K, Bilyk B, Koepff J, Novakova R, van Mellaert L, Oldiges M, Luzhetskyy A, Kormanec J, Anné J, Karamanou S, Economou A.

Front Microbiol. 2018 Dec 7;9:3019. doi: 10.3389/fmicb.2018.03019. eCollection 2018.

9.

Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions.

Koepff J, Sachs CC, Wiechert W, Kohlheyer D, Nöh K, Oldiges M, Grünberger A.

Front Microbiol. 2018 Nov 22;9:2680. doi: 10.3389/fmicb.2018.02680. eCollection 2018.

10.

Secretome Dynamics in a Gram-Positive Bacterial Model.

Tsolis KC, Hamed MB, Simoens K, Koepff J, Busche T, Rückert C, Oldiges M, Kalinowski J, Anné J, Kormanec J, Bernaerts K, Karamanou S, Economou A.

Mol Cell Proteomics. 2019 Mar;18(3):423-436. doi: 10.1074/mcp.RA118.000899. Epub 2018 Nov 29.

PMID:
30498012
11.

Combinatorial impact of Sec signal peptides from Bacillus subtilis and bioprocess conditions on heterologous cutinase secretion by Corynebacterium glutamicum.

Hemmerich J, Moch M, Jurischka S, Wiechert W, Freudl R, Oldiges M.

Biotechnol Bioeng. 2019 Mar;116(3):644-655. doi: 10.1002/bit.26873. Epub 2018 Dec 31.

PMID:
30450544
12.

Less Sacrifice, More Insight: Repeated Low-Volume Sampling of Microbioreactor Cultivations Enables Accelerated Deep Phenotyping of Microbial Strain Libraries.

Hemmerich J, Tenhaef N, Steffens C, Kappelmann J, Weiske M, Reich SJ, Wiechert W, Oldiges M, Noack S.

Biotechnol J. 2019 Sep;14(9):e1800428. doi: 10.1002/biot.201800428. Epub 2018 Nov 19.

PMID:
30318833
13.

Multi-Omics and Targeted Approaches to Determine the Role of Cellular Proteases in Streptomyces Protein Secretion.

Busche T, Tsolis KC, Koepff J, Rebets Y, Rückert C, Hamed MB, Bleidt A, Wiechert W, Lopatniuk M, Yousra A, Anné J, Karamanou S, Oldiges M, Kalinowski J, Luzhetskyy A, Economou A.

Front Microbiol. 2018 Jun 4;9:1174. doi: 10.3389/fmicb.2018.01174. eCollection 2018.

14.

Improved microscale cultivation of Pichia pastoris for clonal screening.

Eck A, Schmidt M, Hamer S, Ruff AJ, Förster J, Schwaneberg U, Blank LM, Wiechert W, Oldiges M.

Fungal Biol Biotechnol. 2018 May 3;5:8. doi: 10.1186/s40694-018-0053-6. eCollection 2018.

15.

Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production.

Kloss R, Limberg MH, Mackfeld U, Hahn D, Grünberger A, Jäger VD, Krauss U, Oldiges M, Pohl M.

Sci Rep. 2018 Apr 11;8(1):5856. doi: 10.1038/s41598-018-24070-2.

16.

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology.

Hemmerich J, Freier L, Wiechert W, von Lieres E, Oldiges M.

J Vis Exp. 2017 Dec 15;(130). doi: 10.3791/56234.

17.

Microbioreactor Systems for Accelerated Bioprocess Development.

Hemmerich J, Noack S, Wiechert W, Oldiges M.

Biotechnol J. 2018 Apr;13(4):e1700141. doi: 10.1002/biot.201700141. Epub 2018 Jan 25. Review.

PMID:
29283217
18.

Automated growth rate determination in high-throughput microbioreactor systems.

Hemmerich J, Wiechert W, Oldiges M.

BMC Res Notes. 2017 Nov 25;10(1):617. doi: 10.1186/s13104-017-2945-6.

19.

Laboratory-scale photobiotechnology-current trends and future perspectives.

Morschett H, Loomba V, Huber G, Wiechert W, von Lieres E, Oldiges M.

FEMS Microbiol Lett. 2018 Jan 1;365(1). doi: 10.1093/femsle/fnx238. Review.

PMID:
29126108
20.

Differential transcriptomic analysis reveals hidden light response in Streptomyces lividans.

Koepff J, Morschett H, Busche T, Winkler A, Kalinowski J, Wiechert W, Oldiges M.

Biotechnol Prog. 2018 Jan;34(1):287-292. doi: 10.1002/btpr.2566. Epub 2017 Nov 3.

PMID:
28960883
21.

pH fluctuations imperil the robustness of C. glutamicum to short term oxygen limitation.

Limberg MH, Joachim M, Klein B, Wiechert W, Oldiges M.

J Biotechnol. 2017 Oct 10;259:248-260. doi: 10.1016/j.jbiotec.2017.08.018. Epub 2017 Aug 22.

PMID:
28837821
22.

Fast and reliable strain characterization of Streptomyces lividans through micro-scale cultivation.

Koepff J, Keller M, Tsolis KC, Busche T, Rückert C, Hamed MB, Anné J, Kalinowski J, Wiechert W, Economou A, Oldiges M.

Biotechnol Bioeng. 2017 Sep;114(9):2011-2022. doi: 10.1002/bit.26321. Epub 2017 May 23.

PMID:
28436005
23.

A framework for accelerated phototrophic bioprocess development: integration of parallelized microscale cultivation, laboratory automation and Kriging-assisted experimental design.

Morschett H, Freier L, Rohde J, Wiechert W, von Lieres E, Oldiges M.

Biotechnol Biofuels. 2017 Jan 31;10:26. doi: 10.1186/s13068-017-0711-6. eCollection 2017.

24.

Comparative evaluation of phototrophic microtiter plate cultivation against laboratory-scale photobioreactors.

Morschett H, Schiprowski D, Rohde J, Wiechert W, Oldiges M.

Bioprocess Biosyst Eng. 2017 May;40(5):663-673. doi: 10.1007/s00449-016-1731-5. Epub 2017 Jan 18.

PMID:
28101644
25.

Use of a Sec signal peptide library from Bacillus subtilis for the optimization of cutinase secretion in Corynebacterium glutamicum.

Hemmerich J, Rohe P, Kleine B, Jurischka S, Wiechert W, Freudl R, Oldiges M.

Microb Cell Fact. 2016 Dec 7;15(1):208.

26.

Current state and challenges for dynamic metabolic modeling.

Vasilakou E, Machado D, Theorell A, Rocha I, Nöh K, Oldiges M, Wahl SA.

Curr Opin Microbiol. 2016 Oct;33:97-104. doi: 10.1016/j.mib.2016.07.008. Epub 2016 Jul 26. Review.

27.

Metabolic profile of 1,5-diaminopentane producing Corynebacterium glutamicum under scale-down conditions: Blueprint for robustness to bioreactor inhomogeneities.

Limberg MH, Schulte J, Aryani T, Mahr R, Baumgart M, Bott M, Wiechert W, Oldiges M.

Biotechnol Bioeng. 2017 Mar;114(3):560-575. doi: 10.1002/bit.26184. Epub 2016 Oct 3.

PMID:
27641904
28.

Distinct purinergic signaling pathways in prepubescent mouse spermatogonia.

Fleck D, Mundt N, Bruentgens F, Geilenkirchen P, Machado PA, Veitinger T, Veitinger S, Lipartowski SM, Engelhardt CH, Oldiges M, Spehr J, Spehr M.

J Gen Physiol. 2016 Sep;148(3):253-71. doi: 10.1085/jgp.201611636.

29.

Design and validation of a parallelized micro-photobioreactor enabling phototrophic bioprocess development at elevated throughput.

Morschett H, Schiprowski D, Müller C, Mertens K, Felden P, Meyer J, Wiechert W, Oldiges M.

Biotechnol Bioeng. 2017 Jan;114(1):122-131. doi: 10.1002/bit.26051. Epub 2016 Jul 26.

PMID:
27424867
30.

Automation of a Nile red staining assay enables high throughput quantification of microalgal lipid production.

Morschett H, Wiechert W, Oldiges M.

Microb Cell Fact. 2016 Feb 9;15:34. doi: 10.1186/s12934-016-0433-7.

31.

(13)C Tracers for Glucose Degrading Pathway Discrimination in Gluconobacter oxydans 621H.

Ostermann S, Richhardt J, Bringer S, Bott M, Wiechert W, Oldiges M.

Metabolites. 2015 Sep 2;5(3):455-74. doi: 10.3390/metabo5030455.

32.

Bioprocess automation on a Mini Pilot Plant enables fast quantitative microbial phenotyping.

Unthan S, Radek A, Wiechert W, Oldiges M, Noack S.

Microb Cell Fact. 2015 Mar 11;14:32. doi: 10.1186/s12934-015-0216-6.

33.

Rapid assessment of oxygen transfer impact for Corynebacterium glutamicum.

Käß F, Prasad A, Tillack J, Moch M, Giese H, Büchs J, Wiechert W, Oldiges M.

Bioprocess Biosyst Eng. 2014 Dec;37(12):2567-77. doi: 10.1007/s00449-014-1234-1. Epub 2014 Jul 1.

PMID:
24981020
34.

Application of metabolic engineering for the biotechnological production of L-valine.

Oldiges M, Eikmanns BJ, Blombach B.

Appl Microbiol Biotechnol. 2014 Jul;98(13):5859-70. doi: 10.1007/s00253-014-5782-8. Epub 2014 May 11. Review.

PMID:
24816722
35.

In vivo assessment by Mach-Zehnder double-beam interferometry of the invasive force exerted by the Asian soybean rust fungus (Phakopsora pachyrhizi).

Loehrer M, Botterweck J, Jahnke J, Mahlmann DM, Gaetgens J, Oldiges M, Horbach R, Deising H, Schaffrath U.

New Phytol. 2014 Jul;203(2):620-31. doi: 10.1111/nph.12784. Epub 2014 Apr 11.

36.

Process inhomogeneity leads to rapid side product turnover in cultivation of Corynebacterium glutamicum.

Käß F, Junne S, Neubauer P, Wiechert W, Oldiges M.

Microb Cell Fact. 2014 Jan 10;13:6. doi: 10.1186/1475-2859-13-6.

37.

Assessment of robustness against dissolved oxygen/substrate oscillations for C. glutamicum DM1933 in two-compartment bioreactor.

Käß F, Hariskos I, Michel A, Brandt HJ, Spann R, Junne S, Wiechert W, Neubauer P, Oldiges M.

Bioprocess Biosyst Eng. 2014 Jun;37(6):1151-62. doi: 10.1007/s00449-013-1086-0. Epub 2013 Nov 12.

PMID:
24218302
38.

Conversion of Corynebacterium glutamicum from an aerobic respiring to an aerobic fermenting bacterium by inactivation of the respiratory chain.

Koch-Koerfges A, Pfelzer N, Platzen L, Oldiges M, Bott M.

Biochim Biophys Acta. 2013 Jun;1827(6):699-708. doi: 10.1016/j.bbabio.2013.02.004. Epub 2013 Feb 15.

39.

An automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform.

Rohe P, Venkanna D, Kleine B, Freudl R, Oldiges M.

Microb Cell Fact. 2012 Oct 31;11:144. doi: 10.1186/1475-2859-11-144.

40.

Polyketide proofreading by an acyltransferase-like enzyme.

Jensen K, Niederkrüger H, Zimmermann K, Vagstad AL, Moldenhauer J, Brendel N, Frank S, Pöplau P, Kohlhaas C, Townsend CA, Oldiges M, Hertweck C, Piel J.

Chem Biol. 2012 Mar 23;19(3):329-39. doi: 10.1016/j.chembiol.2012.01.005.

41.

Direct analysis of underivatized amino acids in plant extracts by LC-MS-MS.

Thiele B, Stein N, Oldiges M, Hofmann D.

Methods Mol Biol. 2012;828:317-28. doi: 10.1007/978-1-61779-445-2_25.

PMID:
22125155
42.

Comparative 13C metabolic flux analysis of pyruvate dehydrogenase complex-deficient, L-valine-producing Corynebacterium glutamicum.

Bartek T, Blombach B, Lang S, Eikmanns BJ, Wiechert W, Oldiges M, Nöh K, Noack S.

Appl Environ Microbiol. 2011 Sep;77(18):6644-52. doi: 10.1128/AEM.00575-11. Epub 2011 Jul 22.

43.

Stationary versus non-stationary (13)C-MFA: a comparison using a consistent dataset.

Noack S, Nöh K, Moch M, Oldiges M, Wiechert W.

J Biotechnol. 2011 Jul 10;154(2-3):179-90. doi: 10.1016/j.jbiotec.2010.07.008. Epub 2010 Jul 16.

PMID:
20638432
44.

Metabolic and transcriptional response to cofactor perturbations in Escherichia coli.

Holm AK, Blank LM, Oldiges M, Schmid A, Solem C, Jensen PR, Vemuri GN.

J Biol Chem. 2010 Jun 4;285(23):17498-506. doi: 10.1074/jbc.M109.095570. Epub 2010 Mar 18.

45.

Studies on substrate utilisation in L-valine-producing Corynebacterium glutamicum strains deficient in pyruvate dehydrogenase complex.

Bartek T, Rudolf C, Kerssen U, Klein B, Blombach B, Lang S, Eikmanns BJ, Oldiges M.

Bioprocess Biosyst Eng. 2010 Sep;33(7):873-83. doi: 10.1007/s00449-010-0410-1. Epub 2010 Mar 5.

PMID:
20204663
46.

Metabolic impact of increased NADH availability in Saccharomyces cerevisiae.

Hou J, Scalcinati G, Oldiges M, Vemuri GN.

Appl Environ Microbiol. 2010 Feb;76(3):851-9. doi: 10.1128/AEM.02040-09. Epub 2009 Dec 18.

47.

Importance of NADPH supply for improved L-valine formation in Corynebacterium glutamicum.

Bartek T, Blombach B, Zönnchen E, Makus P, Lang S, Eikmanns BJ, Oldiges M.

Biotechnol Prog. 2010 Mar-Apr;26(2):361-71. doi: 10.1002/btpr.345.

PMID:
20014412
48.

Analysing overexpression of L-valine biosynthesis genes in pyruvate-dehydrogenase-deficient Corynebacterium glutamicum.

Bartek T, Zönnchen E, Klein B, Gerstmeir R, Makus P, Lang S, Oldiges M.

J Ind Microbiol Biotechnol. 2010 Mar;37(3):263-70. doi: 10.1007/s10295-009-0669-x. Epub 2009 Dec 11.

PMID:
20012552
49.

A 2-oxoacid dehydrogenase complex of Haloferax volcanii is essential for growth on isoleucine but not on other branched-chain amino acids.

Sisignano M, Morbitzer D, Gätgens J, Oldiges M, Soppa J.

Microbiology. 2010 Feb;156(Pt 2):521-9. doi: 10.1099/mic.0.033449-0. Epub 2009 Nov 12.

PMID:
19910413
50.

Application of immobilized bovine enterokinase in repetitive fusion protein cleavage for the production of mucin 1.

Kubitzki T, Minör D, Mackfeld U, Oldiges M, Noll T, Lütz S.

Biotechnol J. 2009 Nov;4(11):1610-8. doi: 10.1002/biot.200900049.

PMID:
19670252

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