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Items: 50

1.

An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system.

Wandera KG, Collins SP, Wimmer F, Marshall R, Noireaux V, Beisel CL.

Methods. 2019 May 21. pii: S1046-2023(19)30001-5. doi: 10.1016/j.ymeth.2019.05.014. [Epub ahead of print]

PMID:
31121300
2.

Characterization of the all-E. coli transcription-translation system myTXTL by mass spectrometry.

Garenne D, Beisel CL, Noireaux V.

Rapid Commun Mass Spectrom. 2019 May 15;33(11):1036-1048. doi: 10.1002/rcm.8438.

PMID:
30900355
3.

TXTL-based approach to synthetic cells.

Garamella J, Garenne D, Noireaux V.

Methods Enzymol. 2019;617:217-239. doi: 10.1016/bs.mie.2018.12.015. Epub 2019 Jan 30.

PMID:
30784403
4.

Distinct timescales of RNA regulators enable the construction of a genetic pulse generator.

Westbrook A, Tang X, Marshall R, Maxwell CS, Chappell J, Agrawal DK, Dunlop MJ, Noireaux V, Beisel CL, Lucks J, Franco E.

Biotechnol Bioeng. 2019 May;116(5):1139-1151. doi: 10.1002/bit.26918. Epub 2019 Feb 4.

PMID:
30636320
5.

Cell-free transcription-translation: engineering biology from the nanometer to the millimeter scale.

Garenne D, Noireaux V.

Curr Opin Biotechnol. 2018 Nov 2;58:19-27. doi: 10.1016/j.copbio.2018.10.007. [Epub ahead of print] Review.

PMID:
30395952
6.

Will biologists become computer scientists? A truly interdisciplinary effort by computer scientists and biologists to understand how cells process information may yield new insights for both fields.

Condon A, Kirchner H, Larivière D, Marshall W, Noireaux V, Tlusty T, Fourmentin E.

EMBO Rep. 2018 Sep;19(9). pii: e46628. doi: 10.15252/embr.201846628. Epub 2018 Jul 30. No abstract available.

PMID:
30061101
7.

Semiconductor Nanoplatelets: A New Class of Ultrabright Fluorescent Probes for Cytometric and Imaging Applications.

Kechkeche D, Cao E, Grazon C, Caschera F, Noireaux V, Baron Niel ML, Dubertret B.

ACS Appl Mater Interfaces. 2018 Jul 25;10(29):24739-24749. doi: 10.1021/acsami.8b07143. Epub 2018 Jul 13.

PMID:
29920060
8.

Synthetic Biology with an All E. coli TXTL System: Quantitative Characterization of Regulatory Elements and Gene Circuits.

Marshall R, Noireaux V.

Methods Mol Biol. 2018;1772:61-93. doi: 10.1007/978-1-4939-7795-6_4.

PMID:
29754223
9.

Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions.

Sakamoto R, Noireaux V, Maeda YT.

Sci Rep. 2018 May 9;8(1):7364. doi: 10.1038/s41598-018-25532-3.

10.

Mathematical Modeling of RNA-Based Architectures for Closed Loop Control of Gene Expression.

Agrawal DK, Tang X, Westbrook A, Marshall R, Maxwell CS, Lucks J, Noireaux V, Beisel CL, Dunlop MJ, Franco E.

ACS Synth Biol. 2018 May 18;7(5):1219-1228. doi: 10.1021/acssynbio.8b00040. Epub 2018 May 8.

PMID:
29709170
11.

A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs.

Maxwell CS, Jacobsen T, Marshall R, Noireaux V, Beisel CL.

Methods. 2018 Jul 1;143:48-57. doi: 10.1016/j.ymeth.2018.02.016. Epub 2018 Feb 24.

PMID:
29486239
12.

Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System.

Marshall R, Maxwell CS, Collins SP, Jacobsen T, Luo ML, Begemann MB, Gray BN, January E, Singer A, He Y, Beisel CL, Noireaux V.

Mol Cell. 2018 Jan 4;69(1):146-157.e3. doi: 10.1016/j.molcel.2017.12.007.

13.

Synchrony and pattern formation of coupled genetic oscillators on a chip of artificial cells.

Tayar AM, Karzbrun E, Noireaux V, Bar-Ziv RH.

Proc Natl Acad Sci U S A. 2017 Oct 31;114(44):11609-11614. doi: 10.1073/pnas.1710620114. Epub 2017 Oct 16.

14.

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System.

Rustad M, Eastlund A, Marshall R, Jardine P, Noireaux V.

J Vis Exp. 2017 Aug 17;(126). doi: 10.3791/56144.

PMID:
28872145
15.

Cell-sized mechanosensitive and biosensing compartment programmed with DNA.

Majumder S, Garamella J, Wang YL, DeNies M, Noireaux V, Liu AP.

Chem Commun (Camb). 2017 Jun 29;53(53):7349-7352. doi: 10.1039/c7cc03455e.

16.

Short DNA containing χ sites enhances DNA stability and gene expression in E. coli cell-free transcription-translation systems.

Marshall R, Maxwell CS, Collins SP, Beisel CL, Noireaux V.

Biotechnol Bioeng. 2017 Sep;114(9):2137-2141. doi: 10.1002/bit.26333. Epub 2017 May 23.

17.

Tuning of Recombinant Protein Expression in Escherichia coli by Manipulating Transcription, Translation Initiation Rates, and Incorporation of Noncanonical Amino Acids.

Schlesinger O, Chemla Y, Heltberg M, Ozer E, Marshall R, Noireaux V, Jensen MH, Alfonta L.

ACS Synth Biol. 2017 Jun 16;6(6):1076-1085. doi: 10.1021/acssynbio.7b00019. Epub 2017 Mar 9.

PMID:
28230975
18.

Expression regulation by a methyl-CpG binding domain in an E. coli based, cell-free TX-TL system.

Schenkelberger M, Shanak S, Finkler M, Worst EG, Noireaux V, Helms V, Ott A.

Phys Biol. 2017 Mar 1;14(2):026002. doi: 10.1088/1478-3975/aa5d37.

PMID:
28140372
19.

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System.

Worst EG, Exner MP, De Simone A, Schenkelberger M, Noireaux V, Budisa N, Ott A.

J Vis Exp. 2016 Aug 1;(114). doi: 10.3791/54273.

20.

Synthetic biology: Tailor-made genetic codes.

Jewett MC, Noireaux V.

Nat Chem. 2016 Apr;8(4):291-2. doi: 10.1038/nchem.2484. No abstract available.

PMID:
27001721
21.

Compartmentalization of an all-E. coli Cell-Free Expression System for the Construction of a Minimal Cell.

Caschera F, Noireaux V.

Artif Life. 2016 Spring;22(2):185-95. doi: 10.1162/ARTL_a_00198. Epub 2016 Mar 2.

PMID:
26934095
22.

The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology.

Garamella J, Marshall R, Rustad M, Noireaux V.

ACS Synth Biol. 2016 Apr 15;5(4):344-55. doi: 10.1021/acssynbio.5b00296. Epub 2016 Feb 9.

PMID:
26818434
23.

[Construction of synthetic cells: from basic questions to applications].

Noireaux V.

Med Sci (Paris). 2015 Dec;31(12):1126-32. doi: 10.1051/medsci/20153112016. Epub 2015 Dec 16. Review. French.

24.

Cell-free expression with the toxic amino acid canavanine.

Worst EG, Exner MP, De Simone A, Schenkelberger M, Noireaux V, Budisa N, Ott A.

Bioorg Med Chem Lett. 2015 Sep 1;25(17):3658-60. doi: 10.1016/j.bmcl.2015.06.045. Epub 2015 Jun 18.

PMID:
26130409
25.

Characterizing and prototyping genetic networks with cell-free transcription-translation reactions.

Takahashi MK, Hayes CA, Chappell J, Sun ZZ, Murray RM, Noireaux V, Lucks JB.

Methods. 2015 Sep 15;86:60-72. doi: 10.1016/j.ymeth.2015.05.020. Epub 2015 May 27.

PMID:
26022922
26.

Genetically expanded cell-free protein synthesis using endogenous pyrrolysyl orthogonal translation system.

Chemla Y, Ozer E, Schlesinger O, Noireaux V, Alfonta L.

Biotechnol Bioeng. 2015 Aug;112(8):1663-72. doi: 10.1002/bit.25587. Epub 2015 Jun 16.

PMID:
25753985
27.

Preparation of amino acid mixtures for cell-free expression systems.

Caschera F, Noireaux V.

Biotechniques. 2015 Jan 1;58(1):40-3. doi: 10.2144/000114249. eCollection 2015 Jan.

28.

A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system.

Caschera F, Noireaux V.

Metab Eng. 2015 Jan;27:29-37. doi: 10.1016/j.ymben.2014.10.007. Epub 2014 Oct 31.

PMID:
25446973
29.

Integration of biological parts toward the synthesis of a minimal cell.

Caschera F, Noireaux V.

Curr Opin Chem Biol. 2014 Oct;22:85-91. doi: 10.1016/j.cbpa.2014.09.028. Epub 2014 Oct 4. Review.

PMID:
25285755
30.

Synthetic biology. Programmable on-chip DNA compartments as artificial cells.

Karzbrun E, Tayar AM, Noireaux V, Bar-Ziv RH.

Science. 2014 Aug 15;345(6198):829-32. doi: 10.1126/science.1255550.

31.

Gene circuit performance characterization and resource usage in a cell-free "breadboard".

Siegal-Gaskins D, Tuza ZA, Kim J, Noireaux V, Murray RM.

ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.

PMID:
24670245
32.

Rapidly characterizing the fast dynamics of RNA genetic circuitry with cell-free transcription-translation (TX-TL) systems.

Takahashi MK, Chappell J, Hayes CA, Sun ZZ, Kim J, Singhal V, Spring KJ, Al-Khabouri S, Fall CP, Noireaux V, Murray RM, Lucks JB.

ACS Synth Biol. 2015 May 15;4(5):503-15. doi: 10.1021/sb400206c. Epub 2014 Mar 28.

33.

Preparation of tethered-lipid bilayers on gold surfaces for the incorporation of integral membrane proteins synthesized by cell-free expression.

Coutable A, Thibault C, Chalmeau J, François JM, Vieu C, Noireaux V, Trévisiol E.

Langmuir. 2014 Mar 25;30(11):3132-41. doi: 10.1021/la5004758. Epub 2014 Mar 11.

PMID:
24568716
34.

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription-translation system.

Caschera F, Noireaux V.

Biochimie. 2014 Apr;99:162-8. doi: 10.1016/j.biochi.2013.11.025. Epub 2013 Dec 8.

PMID:
24326247
35.

Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system.

Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM.

ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.

PMID:
24303785
36.

Protocols for implementing an Escherichia coli based TX-TL cell-free expression system for synthetic biology.

Sun ZZ, Hayes CA, Shin J, Caschera F, Murray RM, Noireaux V.

J Vis Exp. 2013 Sep 16;(79):e50762. doi: 10.3791/50762.

37.

Genome replication, synthesis, and assembly of the bacteriophage T7 in a single cell-free reaction.

Shin J, Jardine P, Noireaux V.

ACS Synth Biol. 2012 Sep 21;1(9):408-13. doi: 10.1021/sb300049p. Epub 2012 Jul 10.

PMID:
23651338
38.

Assembly of MreB filaments on liposome membranes: a synthetic biology approach.

Maeda YT, Nakadai T, Shin J, Uryu K, Noireaux V, Libchaber A.

ACS Synth Biol. 2012 Feb 17;1(2):53-9. doi: 10.1021/sb200003v. Epub 2011 Dec 7.

PMID:
23651045
39.

An E. coli cell-free expression toolbox: application to synthetic gene circuits and artificial cells.

Shin J, Noireaux V.

ACS Synth Biol. 2012 Jan 20;1(1):29-41. doi: 10.1021/sb200016s. Epub 2012 Jan 6.

PMID:
23651008
40.

Coarse-grained dynamics of protein synthesis in a cell-free system.

Karzbrun E, Shin J, Bar-Ziv RH, Noireaux V.

Phys Rev Lett. 2011 Jan 28;106(4):048104. Epub 2011 Jan 24.

PMID:
21405367
41.

Development of an artificial cell, from self-organization to computation and self-reproduction.

Noireaux V, Maeda YT, Libchaber A.

Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3473-80. doi: 10.1073/pnas.1017075108. Epub 2011 Feb 11.

42.

α-Hemolysin pore formation into a supported phospholipid bilayer using cell-free expression.

Chalmeau J, Monina N, Shin J, Vieu C, Noireaux V.

Biochim Biophys Acta. 2011 Jan;1808(1):271-8. doi: 10.1016/j.bbamem.2010.07.027. Epub 2010 Aug 6.

43.
44.

Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70.

Shin J, Noireaux V.

J Biol Eng. 2010 Jun 24;4:8. doi: 10.1186/1754-1611-4-8.

45.

Toward an artificial cell based on gene expression in vesicles.

Noireaux V, Bar-Ziv R, Godefroy J, Salman H, Libchaber A.

Phys Biol. 2005 Sep 15;2(3):P1-8.

PMID:
16224117
46.

A vesicle bioreactor as a step toward an artificial cell assembly.

Noireaux V, Libchaber A.

Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17669-74. Epub 2004 Dec 10.

47.

Principles of cell-free genetic circuit assembly.

Noireaux V, Bar-Ziv R, Libchaber A.

Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12672-7. Epub 2003 Oct 14.

48.

In vivo imaging of quantum dots encapsulated in phospholipid micelles.

Dubertret B, Skourides P, Norris DJ, Noireaux V, Brivanlou AH, Libchaber A.

Science. 2002 Nov 29;298(5599):1759-62.

49.

ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity.

Fradelizi J, Noireaux V, Plastino J, Menichi B, Louvard D, Sykes C, Golsteyn RM, Friederich E.

Nat Cell Biol. 2001 Aug;3(8):699-707.

PMID:
11483954
50.

Growing an actin gel on spherical surfaces.

Noireaux V, Golsteyn RM, Friederich E, Prost J, Antony C, Louvard D, Sykes C.

Biophys J. 2000 Mar;78(3):1643-54.

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