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J Microbiol Methods. 2019 Feb;157:1-3. doi: 10.1016/j.mimet.2018.12.010. Epub 2018 Dec 14.

High fidelity recovery of airborne microbial genetic materials by direct condensation capture into genomic preservatives.

Author information

1
University of Colorado, Environmental Engineering Program, Boulder, CO, USA.
2
Aerosol Devices Inc., Fort Collins, CO, USA.
3
University of Colorado, Environmental Engineering Program, Boulder, CO, USA. Electronic address: mark.hernandez@colorado.edu.

Abstract

A condensation growth tube was adapted to capture bacterial bioaerosols directly into genomic preservatives. As judged by quantitative PCR and direct microscopy, bioaerosol condensation capture conserves airborne microbes' genomes as they exist in the atmospheric environment. This method circumvents the collection stresses bioaerosols experience on air filters, impactors and impingers.

PMID:
30557586
DOI:
10.1016/j.mimet.2018.12.010
[Indexed for MEDLINE]

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