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Items: 1 to 50 of 250

1.

Reconstitution of 30S ribosomal subunits in vitro using ribosome biogenesis factors.

Tamaru D, Amikura K, Shimizu Y, Nierhaus KH, Ueda T.

RNA. 2018 Nov;24(11):1512-1519. doi: 10.1261/rna.065615.118. Epub 2018 Aug 3.

PMID:
30076205
2.

Structural Visualization of the Formation and Activation of the 50S Ribosomal Subunit during In Vitro Reconstitution.

Nikolay R, Hilal T, Qin B, Mielke T, Bürger J, Loerke J, Textoris-Taube K, Nierhaus KH, Spahn CMT.

Mol Cell. 2018 Jun 7;70(5):881-893.e3. doi: 10.1016/j.molcel.2018.05.003. Epub 2018 Jun 7.

3.

Translation and folding of single proteins in real time.

Wruck F, Katranidis A, Nierhaus KH, Büldt G, Hegner M.

Proc Natl Acad Sci U S A. 2017 May 30;114(22):E4399-E4407. doi: 10.1073/pnas.1617873114. Epub 2017 May 15.

4.

The Termination Phase in Protein Synthesis is not Obligatorily Followed by the RRF/EF-G-Dependent Recycling Phase.

Qin B, Yamamoto H, Ueda T, Varshney U, Nierhaus KH.

J Mol Biol. 2016 Sep 11;428(18):3577-87. doi: 10.1016/j.jmb.2016.05.019. Epub 2016 May 31.

PMID:
27261258
5.

Ribosome Assembly as Antimicrobial Target.

Nikolay R, Schmidt S, Schlömer R, Deuerling E, Nierhaus KH.

Antibiotics (Basel). 2016 May 27;5(2). pii: E18. doi: 10.3390/antibiotics5020018.

6.

70S-scanning initiation is a novel and frequent initiation mode of ribosomal translation in bacteria.

Yamamoto H, Wittek D, Gupta R, Qin B, Ueda T, Krause R, Yamamoto K, Albrecht R, Pech M, Nierhaus KH.

Proc Natl Acad Sci U S A. 2016 Mar 1;113(9):E1180-9. doi: 10.1073/pnas.1524554113. Epub 2016 Feb 17.

7.
8.

Outwitting EF-Tu and the ribosome: translation with d-amino acids.

Achenbach J, Jahnz M, Bethge L, Paal K, Jung M, Schuster M, Albrecht R, Jarosch F, Nierhaus KH, Klussmann S.

Nucleic Acids Res. 2015 Jul 13;43(12):5687-98. doi: 10.1093/nar/gkv566. Epub 2015 May 30.

9.

The mechanics of ribosomal translocation.

Achenbach J, Nierhaus KH.

Biochimie. 2015 Jul;114:80-9. doi: 10.1016/j.biochi.2014.12.003. Epub 2014 Dec 13. Review.

PMID:
25514765
10.

Ribosome rescue and translation termination at non-standard stop codons by ICT1 in mammalian mitochondria.

Akabane S, Ueda T, Nierhaus KH, Takeuchi N.

PLoS Genet. 2014 Sep 18;10(9):e1004616. doi: 10.1371/journal.pgen.1004616. eCollection 2014 Sep.

11.

Mg2+, K+, and the ribosome.

Nierhaus KH.

J Bacteriol. 2014 Nov;196(22):3817-9. doi: 10.1128/JB.02297-14. Epub 2014 Sep 15.

12.

The CCA-end of P-tRNA Contacts Both the Human RPL36AL and the A-site Bound Translation Termination Factor eRF1 at the Peptidyl Transferase Center of the Human 80S Ribosome.

Hountondji C, Bulygin K, Créchet JB, Woisard A, Tuffery P, Nakayama J, Frolova L, Nierhaus KH, Karpova G, Baouz S.

Open Biochem J. 2014 Jul 11;8:52-67. doi: 10.2174/1874091X01408010052. eCollection 2014.

13.

EF-G catalyzes tRNA translocation by disrupting interactions between decoding center and codon-anticodon duplex.

Liu G, Song G, Zhang D, Zhang D, Li Z, Lyu Z, Dong J, Achenbach J, Gong W, Zhao XS, Nierhaus KH, Qin Y.

Nat Struct Mol Biol. 2014 Sep;21(9):817-24. doi: 10.1038/nsmb.2869. Epub 2014 Aug 10.

PMID:
25108354
14.

Regulation of the mammalian elongation cycle by subunit rolling: a eukaryotic-specific ribosome rearrangement.

Budkevich TV, Giesebrecht J, Behrmann E, Loerke J, Ramrath DJ, Mielke T, Ismer J, Hildebrand PW, Tung CS, Nierhaus KH, Sanbonmatsu KY, Spahn CM.

Cell. 2014 Jul 3;158(1):121-31. doi: 10.1016/j.cell.2014.04.044.

15.

EF-G and EF4: translocation and back-translocation on the bacterial ribosome.

Yamamoto H, Qin Y, Achenbach J, Li C, Kijek J, Spahn CM, Nierhaus KH.

Nat Rev Microbiol. 2014 Feb;12(2):89-100. doi: 10.1038/nrmicro3176. Epub 2013 Dec 23. Review.

PMID:
24362468
16.

Translocation at work.

Achenbach J, Nierhaus KH.

Nat Struct Mol Biol. 2013 Sep;20(9):1019-22. doi: 10.1038/nsmb.2661. No abstract available.

PMID:
24008560
17.

Common chaperone activity in the G-domain of trGTPase protects L11-L12 interaction on the ribosome.

Zhang D, Liu G, Xue J, Lou J, Nierhaus KH, Gong W, Qin Y.

Nucleic Acids Res. 2012 Nov;40(21):10851-65. doi: 10.1093/nar/gks833. Epub 2012 Sep 10.

18.

Three mechanisms in Escherichia coli rescue ribosomes stalled on non-stop mRNAs: one of them requires release factor 2.

Pech M, Nierhaus KH.

Mol Microbiol. 2012 Oct;86(1):6-9. doi: 10.1111/j.1365-2958.2012.08207.x. Epub 2012 Aug 22.

19.

Problems with the Analyses of the Ribosomal Allosteric Three-site Model.

Nierhaus KH, Pech M.

J Biol Chem. 2012 Aug 3;287(32):27049. doi: 10.1074/jbc.L112.381848. No abstract available.

20.

Lys53 of ribosomal protein L36AL and the CCA end of a tRNA at the P/E hybrid site are in close proximity on the human ribosome.

Hountondji C, Bulygin K, Woisard A, Tuffery P, Créchet JB, Pech M, Nierhaus KH, Karpova G, Baouz S.

Chembiochem. 2012 Aug 13;13(12):1791-7. doi: 10.1002/cbic.201200208.

PMID:
22865768
21.

RsfA (YbeB) proteins are conserved ribosomal silencing factors.

Häuser R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, Tovchigrechko A, Yamamoto K, Szaflarski W, Takeuchi N, Stellberger T, Diefenbacher ME, Nierhaus KH, Uetz P.

PLoS Genet. 2012;8(7):e1002815. doi: 10.1371/journal.pgen.1002815. Epub 2012 Jul 19.

22.

The complex of tmRNA-SmpB and EF-G on translocating ribosomes.

Ramrath DJ, Yamamoto H, Rother K, Wittek D, Pech M, Mielke T, Loerke J, Scheerer P, Ivanov P, Teraoka Y, Shpanchenko O, Nierhaus KH, Spahn CM.

Nature. 2012 May 6;485(7399):526-9. doi: 10.1038/nature11006.

PMID:
22622583
23.

A conserved proline switch on the ribosome facilitates the recruitment and binding of trGTPases.

Wang L, Yang F, Zhang D, Chen Z, Xu RM, Nierhaus KH, Gong W, Qin Y.

Nat Struct Mol Biol. 2012 Mar 11;19(4):403-10. doi: 10.1038/nsmb.2254.

PMID:
22407015
24.

Identical binding sites--nonidentical functions in Eukarya and Archaea: the complex of aeIF6 with the large ribosomal subunit.

Pech M, Nierhaus KH.

J Mol Biol. 2012 May 4;418(3-4):131-3. doi: 10.1016/j.jmb.2012.02.033. Epub 2012 Feb 27. No abstract available.

25.

The thorny way to the mechanism of ribosomal peptide-bond formation.

Pech M, Nierhaus KH.

Chembiochem. 2012 Jan 23;13(2):189-92. doi: 10.1002/cbic.201100660. Epub 2012 Jan 2. No abstract available.

PMID:
22213275
26.

Structure and dynamics of the mammalian ribosomal pretranslocation complex.

Budkevich T, Giesebrecht J, Altman RB, Munro JB, Mielke T, Nierhaus KH, Blanchard SC, Spahn CM.

Mol Cell. 2011 Oct 21;44(2):214-24. doi: 10.1016/j.molcel.2011.07.040.

27.

Structural basis for the binding of IRES RNAs to the head of the ribosomal 40S subunit.

Muhs M, Yamamoto H, Ismer J, Takaku H, Nashimoto M, Uchiumi T, Nakashima N, Mielke T, Hildebrand PW, Nierhaus KH, Spahn CM.

Nucleic Acids Res. 2011 Jul;39(12):5264-75. doi: 10.1093/nar/gkr114. Epub 2011 Mar 4.

28.

Elongation factor 4 (EF4/LepA) accelerates protein synthesis at increased Mg2+ concentrations.

Pech M, Karim Z, Yamamoto H, Kitakawa M, Qin Y, Nierhaus KH.

Proc Natl Acad Sci U S A. 2011 Feb 22;108(8):3199-203. doi: 10.1073/pnas.1012994108. Epub 2011 Feb 7.

29.

NOA1 is an essential GTPase required for mitochondrial protein synthesis.

Kolanczyk M, Pech M, Zemojtel T, Yamamoto H, Mikula I, Calvaruso MA, van den Brand M, Richter R, Fischer B, Ritz A, Kossler N, Thurisch B, Spoerle R, Smeitink J, Kornak U, Chan D, Vingron M, Martasek P, Lightowlers RN, Nijtmans L, Schuelke M, Nierhaus KH, Mundlos S.

Mol Biol Cell. 2011 Jan 1;22(1):1-11. doi: 10.1091/mbc.E10-07-0643. Epub 2010 Nov 30.

30.

Nobel Prize for the elucidation of ribosome structure and insight into the translation mechanism.

Nierhaus KH.

Angew Chem Int Ed Engl. 2009;48(49):9225-8. doi: 10.1002/anie.200905795. No abstract available.

PMID:
19899182
31.

EF-G2mt is an exclusive recycling factor in mammalian mitochondrial protein synthesis.

Tsuboi M, Morita H, Nozaki Y, Akama K, Ueda T, Ito K, Nierhaus KH, Takeuchi N.

Mol Cell. 2009 Aug 28;35(4):502-10. doi: 10.1016/j.molcel.2009.06.028.

32.

An unexpected type of ribosomes induced by kasugamycin: a look into ancestral times of protein synthesis?

Kaberdina AC, Szaflarski W, Nierhaus KH, Moll I.

Mol Cell. 2009 Jan 30;33(2):227-36. doi: 10.1016/j.molcel.2008.12.014.

33.

Fast biosynthesis of GFP molecules: a single-molecule fluorescence study.

Katranidis A, Atta D, Schlesinger R, Nierhaus KH, Choli-Papadopoulou T, Gregor I, Gerrits M, Büldt G, Fitter J.

Angew Chem Int Ed Engl. 2009;48(10):1758-61. doi: 10.1002/anie.200806070.

PMID:
19173359
34.

A new tRNA intermediate revealed on the ribosome during EF4-mediated back-translocation.

Connell SR, Topf M, Qin Y, Wilson DN, Mielke T, Fucini P, Nierhaus KH, Spahn CM.

Nat Struct Mol Biol. 2008 Sep;15(9):910-5.

PMID:
19172743
35.

Small RNA binding to 5' mRNA coding region inhibits translational initiation.

Bouvier M, Sharma CM, Mika F, Nierhaus KH, Vogel J.

Mol Cell. 2008 Dec 26;32(6):827-37. doi: 10.1016/j.molcel.2008.10.027.

36.

In vitro selection of random RNA fragments to identify protein-binding sites within large RNAs.

Stelzl U, Nierhaus KH.

Methods Mol Biol. 2008;488:247-55. doi: 10.1007/978-1-60327-475-3_17.

PMID:
18982296
37.

Characterization of RNA-protein interactions by phosphorothioate footprinting and its applications to the ribosome.

Ozlem Tastan Bishop A, Stelzl U, Pech M, Nierhaus KH.

Methods Mol Biol. 2008;488:129-51. doi: 10.1007/978-1-60327-475-3_9.

PMID:
18982288
38.

Shine-Dalgarno interaction prevents incorporation of noncognate amino acids at the codon following the AUG.

Di Giacco V, Márquez V, Qin Y, Pech M, Triana-Alonso FJ, Wilson DN, Nierhaus KH.

Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10715-20. doi: 10.1073/pnas.0801974105. Epub 2008 Jul 30.

39.

Features of 80S mammalian ribosome and its subunits.

Budkevich TV, El'skaya AV, Nierhaus KH.

Nucleic Acids Res. 2008 Aug;36(14):4736-44. doi: 10.1093/nar/gkn424. Epub 2008 Jul 16.

40.

New features of the ribosome and ribosomal inhibitors: non-enzymatic recycling, misreading and back-translocation.

Szaflarski W, Vesper O, Teraoka Y, Plitta B, Wilson DN, Nierhaus KH.

J Mol Biol. 2008 Jun 27;380(1):193-205. doi: 10.1016/j.jmb.2008.04.060. Epub 2008 May 2.

PMID:
18508080
41.

Ribosomal peptide-bond formation.

Pech M, Nierhaus KH.

Chem Biol. 2008 May;15(5):417-9. doi: 10.1016/j.chembiol.2008.04.008.

42.

Question 6: early steps of evolution and some ideas about a simplified translational machinery.

Nierhaus KH.

Orig Life Evol Biosph. 2007 Oct;37(4-5):391-8. Epub 2007 Aug 1.

PMID:
17668285
43.

Question 7: optimized energy consumption for protein synthesis.

Szaflarski W, Nierhaus KH.

Orig Life Evol Biosph. 2007 Oct;37(4-5):423-8. Epub 2007 Jul 18.

PMID:
17634746
44.

The weird and wonderful world of bacterial ribosome regulation.

Wilson DN, Nierhaus KH.

Crit Rev Biochem Mol Biol. 2007 May-Jun;42(3):187-219. Review.

PMID:
17562451
45.

The oxazolidinone class of drugs find their orientation on the ribosome.

Wilson DN, Nierhaus KH.

Mol Cell. 2007 May 25;26(4):460-2. No abstract available.

46.

The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome.

Qin Y, Polacek N, Vesper O, Staub E, Einfeldt E, Wilson DN, Nierhaus KH.

Cell. 2006 Nov 17;127(4):721-33.

47.

Troubleshooting coupled in vitro transcription-translation system derived from Escherichia coli cells: synthesis of high-yield fully active proteins.

Iskakova MB, Szaflarski W, Dreyfus M, Remme J, Nierhaus KH.

Nucleic Acids Res. 2006;34(19):e135. Epub 2006 Oct 11.

48.

The E-site story: the importance of maintaining two tRNAs on the ribosome during protein synthesis.

Wilson DN, Nierhaus KH.

Cell Mol Life Sci. 2006 Dec;63(23):2725-37. Review.

PMID:
17013564
49.

The antibiotic kasugamycin mimics mRNA nucleotides to destabilize tRNA binding and inhibit canonical translation initiation.

Schluenzen F, Takemoto C, Wilson DN, Kaminishi T, Harms JM, Hanawa-Suetsugu K, Szaflarski W, Kawazoe M, Shirouzu M, Nierhaus KH, Yokoyama S, Fucini P.

Nat Struct Mol Biol. 2006 Oct;13(10):871-8. Epub 2006 Sep 24. Erratum in: Nat Struct Mol Biol. 2006 Nov;13(11):1033. Shirouzo, Mikako [corrected to Shirouzu, Mikako].

PMID:
16998488
50.

Decoding errors and the involvement of the E-site.

Nierhaus KH.

Biochimie. 2006 Aug;88(8):1013-9. Epub 2006 Mar 23. Review.

PMID:
16644089

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