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eNeuro. 2018 May 17;5(2). pii: ENEURO.0387-17.2018. doi: 10.1523/ENEURO.0387-17.2018. eCollection 2018 Mar-Apr.

Altered Cerebellar Short-Term Plasticity but No Change in Postsynaptic AMPA-Type Glutamate Receptors in a Mouse Model of Juvenile Batten Disease.

Author information

1
Department of Neuroscience, Physiology and Pharmacology, University College London, London WC1E 6BT, United Kingdom.
2
UCL Great Ormond Street Institute of Child Health, University College London, London WC1N 1EH, United Kingdom.

Abstract

Juvenile Batten disease is the most common progressive neurodegenerative disorder of childhood. It is associated with mutations in the CLN3 gene, causing loss of function of CLN3 protein and degeneration of cerebellar and retinal neurons. It has been proposed that changes in granule cell AMPA-type glutamate receptors (AMPARs) contribute to the cerebellar dysfunction. In this study, we compared AMPAR properties and synaptic transmission in cerebellar granule cells from wild-type and Cln3 knock-out mice. In Cln3Δex1-6 cells, the amplitude of AMPA-evoked whole-cell currents was unchanged. Similarly, we found no change in the amplitude, kinetics, or rectification of synaptic currents evoked by individual quanta, or in their underlying single-channel conductance. We found no change in cerebellar expression of GluA2 or GluA4 protein. By contrast, we observed a reduced number of quantal events following mossy-fiber stimulation in Sr2+, altered short-term plasticity in conditions of reduced extracellular Ca2+, and reduced mossy fiber vesicle number. Thus, while our results suggest early presynaptic changes in the Cln3Δex1-6 mouse model of juvenile Batten disease, they reveal no evidence for altered postsynaptic AMPARs.

KEYWORDS:

AMPA receptors; Batten disease; CLN3; EPSCs; cerebellum; short-term plasticity

PMID:
29780879
PMCID:
PMC5956745
DOI:
10.1523/ENEURO.0387-17.2018
[Indexed for MEDLINE]
Free PMC Article

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