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Elife. 2018 Sep 7;7. pii: e37892. doi: 10.7554/eLife.37892.

DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis.

Author information

1
Centro Nacional de Biotecnología, CNB-CSIC, Madrid, Spain.
2
Institut de biologie de l'Ecole normale supérieure (IBENS), Ecole normale supérieure, CNRS, INSERM, Université PSL, Paris, France.
3
Université Paris-Sud, Orsay, France.
4
Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie PSL Research University, 75005 Paris, France.
5
Department of Plant Systems Biology, Ghent University, Ghent, Belgium.
6
VIB Center for Plant Systems Biology, Ghent, Belgium.
7
Department of Biochemistry, Ghent University, Ghent, Belgium.
8
VIB Center for Medical Biotechnology, Ghent, Belgium.
9
Université Grenoble Alpes, Institut de Biologie Structurale, Grenoble, France.
#
Contributed equally

Abstract

DE-ETIOLATED 1 (DET1) is an evolutionarily conserved component of the ubiquitination machinery that mediates the destabilization of key regulators of cell differentiation and proliferation in multicellular organisms. In this study, we provide evidence from Arabidopsis that DET1 is essential for the regulation of histone H2B monoubiquitination (H2Bub) over most genes by controlling the stability of a deubiquitination module (DUBm). In contrast with yeast and metazoan DUB modules that are associated with the large SAGA complex, the Arabidopsis DUBm only comprises three proteins (hereafter named SGF11, ENY2 and UBP22) and appears to act independently as a major H2Bub deubiquitinase activity. Our study further unveils that DET1-DDB1-Associated-1 (DDA1) protein interacts with SGF11 in vivo, linking the DET1 complex to light-dependent ubiquitin-mediated proteolytic degradation of the DUBm. Collectively, these findings uncover a signaling path controlling DUBm availability, potentially adjusting H2Bub turnover capacity to the cell transcriptional status.

KEYWORDS:

A. thaliana; DET1; Histone H2B deubiquitination; SAGA complex; epigenome; light signaling; photomorphogenesis; plant biology

PMID:
30192741
PMCID:
PMC6128693
DOI:
10.7554/eLife.37892
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

AN, MR, CB, SD, SF, EI, OA, AD, GZ, IA, DS, VM, BL, DE, KG, DL, AG, CB, Gd, CB, VR, FB No competing interests declared

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