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Items: 1 to 50 of 211

1.

An epitope-directed antibody affinity maturation system utilizing mammalian cell survival as readout.

Eguchi A, Nakakido M, Nagatoishi S, Kuroda D, Tsumoto K, Nagamune T, Kawahara M.

Biotechnol Bioeng. 2019 Jul;116(7):1742-1751. doi: 10.1002/bit.26965. Epub 2019 Mar 29.

PMID:
30883677
2.

A Suicide Switch Directly Eliminates Intracellular scFv Oligomers in the Cytoplasm of Mammalian Cells.

Nguyen TD, Nagamune T, Kawahara M.

Biotechnol J. 2019 Jan;14(1):e1800350. doi: 10.1002/biot.201800350. Epub 2018 Sep 20.

PMID:
30171736
3.

Stepwise phosphorylation of leukotriene B4 receptor 1 defines cellular responses to leukotriene B4.

Nakanishi Y, Tan M, Ichiki T, Inoue A, Yoshihara JI, Maekawa N, Takenoshita I, Yanagida K, Yamahira S, Yamaguchi S, Aoki J, Nagamune T, Yokomizo T, Shimizu T, Nakamura M.

Sci Signal. 2018 Aug 21;11(544). pii: eaao5390. doi: 10.1126/scisignal.aao5390.

PMID:
30131369
4.

Artificial Self-Sufficient Cytochrome P450 Containing Multiple Auxiliary Proteins Demonstrates Improved Monooxygenase Activity.

Haga T, Hirakawa H, Nagamune T.

Biotechnol J. 2018 Dec;13(12):e1800088. doi: 10.1002/biot.201800088. Epub 2018 Aug 14.

PMID:
30039932
5.

Designing Motif-Engineered Receptors To Elucidate Signaling Molecules Important for Proliferation of Hematopoietic Stem Cells.

Ishizuka S, Lai CY, Otsu M, Nakauchi H, Nagamune T, Kawahara M.

ACS Synth Biol. 2018 Jul 20;7(7):1709-1714. doi: 10.1021/acssynbio.8b00163. Epub 2018 Jun 21.

PMID:
29920201
6.

A Stable Artificial Multienzymatic Complex Using a Heterotrimeric Protein From Metallosphaera sedula.

Iwata F, Hirakawa H, Nagamune T.

Biotechnol J. 2018 Nov;13(11):e1700662. doi: 10.1002/biot.201700662. Epub 2018 Apr 30.

PMID:
29663675
7.

Real-time monitoring of pH-dependent intracellular trafficking of ovarian cancer G protein-coupled receptor 1 in living leukocytes.

Tan M, Yamaguchi S, Nakamura M, Nagamune T.

J Biosci Bioeng. 2018 Sep;126(3):363-370. doi: 10.1016/j.jbiosc.2018.03.012. Epub 2018 Apr 11.

PMID:
29655915
8.

Microfluidic preparation of anchored cell membrane sheets for in vitro analyses and manipulation of the cytoplasmic face.

Izuta S, Yamaguchi S, Misawa R, Yamahira S, Tan M, Kawahara M, Suzuki T, Takagi T, Sato K, Nakamura M, Nagamune T, Okamoto A.

Sci Rep. 2017 Nov 2;7(1):14962. doi: 10.1038/s41598-017-14737-7.

9.

Dissection of Signaling Events Downstream of the c-Mpl Receptor in Murine Hematopoietic Stem Cells Via Motif-Engineered Chimeric Receptors.

Saka K, Lai CY, Nojima M, Kawahara M, Otsu M, Nakauchi H, Nagamune T.

Stem Cell Rev Rep. 2018 Feb;14(1):101-109. doi: 10.1007/s12015-017-9768-7.

10.

Cell-Surface Expression Levels Are Important for Fine-Tuning the Performance of Receptor Tyrosine Kinase-Based Signalobodies.

Nakabayashi H, Kawahara M, Nagamune T.

Biotechnol J. 2017 Dec;12(12). doi: 10.1002/biot.201700441. Epub 2017 Sep 25.

PMID:
28881109
11.

Biomolecular engineering for nanobio/bionanotechnology.

Nagamune T.

Nano Converg. 2017;4(1):9. doi: 10.1186/s40580-017-0103-4. Epub 2017 Apr 24. Review.

12.

Quantitative image cytometry for analyzing intracellular trafficking of G protein-coupled receptors on a chemical-trapping single cell array.

Tan M, Yamaguchi S, Yamahira S, Nakamura M, Nagamune T.

Lab Chip. 2017 May 31;17(11):1933-1938. doi: 10.1039/c7lc00198c.

PMID:
28475195
13.

A Chemically Inducible Helper Module for Detecting Protein-Protein Interactions with Tunable Sensitivity Based on KIPPIS.

Kashima D, Kawade R, Nagamune T, Kawahara M.

Anal Chem. 2017 May 2;89(9):4824-4830. doi: 10.1021/acs.analchem.6b04063. Epub 2017 Apr 13.

PMID:
28382822
14.

Selective intracellular vaporisation of antibody-conjugated phase-change nano-droplets in vitro.

Ishijima A, Minamihata K, Yamaguchi S, Yamahira S, Ichikawa R, Kobayashi E, Iijima M, Shibasaki Y, Azuma T, Nagamune T, Sakuma I.

Sci Rep. 2017 Mar 23;7:44077. doi: 10.1038/srep44077.

15.

Engineering a growth sensor to select intracellular antibodies in the cytosol of mammalian cells.

Nguyen TD, Takasuka H, Kaku Y, Inoue S, Nagamune T, Kawahara M.

J Biosci Bioeng. 2017 Jul;124(1):125-132. doi: 10.1016/j.jbiosc.2017.02.017. Epub 2017 Mar 17.

PMID:
28319021
16.

Protein refolding is improved by adding nonionic polyethylene glycol monooleyl ethers with various polyethylene glycol lengths.

Yamamoto E, Yamaguchi S, Nagamune T.

Biotechnol J. 2017 May;12(5). doi: 10.1002/biot.201600689. Epub 2017 Apr 6.

PMID:
28263439
17.

Correction to Peptide Tag-Induced Horseradish Peroxidase-Mediated Preparation of a Streptavidin-Immobilized Redox-Sensitive Hydrogel.

Mishina M, Minamihata K, Moriyama K, Nagamune T.

Biomacromolecules. 2017 Jan 9;18(1):311. doi: 10.1021/acs.biomac.6b01728. Epub 2016 Dec 21. No abstract available.

PMID:
28001047
18.

Immobilization of a Bacterial Cytochrome P450 Monooxygenase System on a Solid Support.

Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T.

Angew Chem Int Ed Engl. 2016 Nov 21;55(48):15002-15006. doi: 10.1002/anie.201608033. Epub 2016 Oct 26.

PMID:
27781345
19.

Cell-Based Odorant Sensor Array for Odor Discrimination Based on Insect Odorant Receptors.

Termtanasombat M, Mitsuno H, Misawa N, Yamahira S, Sakurai T, Yamaguchi S, Nagamune T, Kanzaki R.

J Chem Ecol. 2016 Jul;42(7):716-24. doi: 10.1007/s10886-016-0726-7. Epub 2016 Jul 16.

PMID:
27424160
20.

Three proliferating cell nuclear antigen homologues from Metallosphaera sedula form a head-to-tail heterotrimer.

Iwata F, Hirakawa H, Nagamune T.

Sci Rep. 2016 May 27;6:26588. doi: 10.1038/srep26588.

21.

Peptide Tag-Induced Horseradish Peroxidase-Mediated Preparation of a Streptavidin-Immobilized Redox-Sensitive Hydrogel.

Mishina M, Minamihata K, Moriyama K, Nagamune T.

Biomacromolecules. 2016 Jun 13;17(6):1978-84. doi: 10.1021/acs.biomac.6b00149. Epub 2016 May 26. Erratum in: Biomacromolecules. 2017 Jan 9;18(1):311.

PMID:
27183298
22.

Tyrosine Coupling Creates a Hyperbranched Multivalent Protein Polymer Using Horseradish Peroxidase via Bipolar Conjugation Points.

Minamihata K, Yamaguchi S, Nakajima K, Nagamune T.

Bioconjug Chem. 2016 May 18;27(5):1348-59. doi: 10.1021/acs.bioconjchem.6b00138. Epub 2016 Apr 29.

PMID:
27093089
23.

The lifetime evaluation of vapourised phase-change nano-droplets.

Ishijima A, Tanaka J, Azuma T, Minamihata K, Yamaguchi S, Kobayashi E, Nagamune T, Sakuma I.

Ultrasonics. 2016 Jul;69:97-105. doi: 10.1016/j.ultras.2016.04.002. Epub 2016 Apr 4.

24.

Differentiation signalobody: Demonstration of antigen-dependent osteoclast differentiation from a progenitor cell line.

Nakabayashi H, Aoyama S, Kawahara M, Nagamune T.

J Biosci Bioeng. 2016 Sep;122(3):357-63. doi: 10.1016/j.jbiosc.2016.02.010. Epub 2016 Mar 12.

PMID:
26979343
25.

Photolytic Protein Aggregates: Versatile Materials for Controlled Release of Active Proteins.

Ishiwatari A, Yamaguchi S, Takamori S, Yamahira S, Minamihata K, Nagamune T.

Adv Healthc Mater. 2016 May;5(9):1002-7. doi: 10.1002/adhm.201500957. Epub 2016 Mar 4.

PMID:
26945901
26.

Growth signalobody selects functional intrabodies in the mammalian cytoplasm.

Lee S, Kaku Y, Inoue S, Nagamune T, Kawahara M.

Biotechnol J. 2016 Mar;11(4):565-73. doi: 10.1002/biot.201500364. Epub 2016 Jan 14.

PMID:
26647155
27.

Ligand-inducible dimeric antibody for selecting antibodies against a membrane protein based on mammalian cell proliferation.

Miura T, Nagamune T, Kawahara M.

Biotechnol Bioeng. 2016 May;113(5):1113-23. doi: 10.1002/bit.25858. Epub 2015 Oct 26.

PMID:
26479395
28.

Photosensitizer and polycationic peptide-labeled streptavidin as a nano-carrier for light-controlled protein transduction.

Minamihata K, Maeda Y, Yamaguchi S, Ishihara W, Ishiwatari A, Takamori S, Yamahira S, Nagamune T.

J Biosci Bioeng. 2015 Dec;120(6):630-6. doi: 10.1016/j.jbiosc.2015.04.001. Epub 2015 Apr 29.

PMID:
25935501
29.

Sortase A-mediated synthesis of ligand-grafted cyclized peptides for modulating a model protein-protein interaction.

Zhang J, Yamaguchi S, Nagamune T.

Biotechnol J. 2015 Sep;10(9):1499-505. doi: 10.1002/biot.201500013. Epub 2015 May 26.

PMID:
25913771
30.

Ca2+ -independent sortase-A exhibits high selective protein ligation activity in the cytoplasm of Escherichia coli.

Hirakawa H, Ishikawa S, Nagamune T.

Biotechnol J. 2015 Sep;10(9):1487-92. doi: 10.1002/biot.201500012. Epub 2015 Apr 28.

PMID:
25864513
31.
32.

Selection of cDNA candidates that induce oligomerization of NLRP3 using a chimeric receptor approach.

Honda S, Nagamune T, Kawahara M.

J Biosci Bioeng. 2015 Aug;120(2):223-30. doi: 10.1016/j.jbiosc.2014.12.021. Epub 2015 Jan 29.

PMID:
25641579
33.

Domain structure of growth signalobodies critically affects the outcome of antibody library selection.

Yoshida R, Kawahara M, Nagamune T.

J Biochem. 2015 Jun;157(6):497-506. doi: 10.1093/jb/mvv008. Epub 2015 Jan 22.

PMID:
25616678
34.

Collagen surfaces modified with photo-cleavable polyethylene glycol-lipid support versatile single-cell arrays of both non-adherent and adherent cells.

Yamahira S, Yamaguchi S, Kawahara M, Nagamune T.

Macromol Biosci. 2014 Dec;14(12):1670-6. doi: 10.1002/mabi.201400312. Epub 2014 Sep 5.

PMID:
25195937
35.

Detecting protein-protein interactions based on kinase-mediated growth induction of mammalian cells.

Mabe S, Nagamune T, Kawahara M.

Sci Rep. 2014 Aug 19;4:6127. doi: 10.1038/srep06127.

36.

Fusion protein bilayer fabrication composed of recombinant azurin/cytochrome P450 by the sortase-mediated ligation method.

Lee T, Min J, Hirakawa H, Nagamune T, Choi JW.

Colloids Surf B Biointerfaces. 2014 Aug 1;120:215-21. doi: 10.1016/j.colsurfb.2014.03.034. Epub 2014 May 21.

PMID:
24924834
37.

Electron donation to an archaeal cytochrome P450 is enhanced by PCNA-mediated selective complex formation with foreign redox proteins.

Suzuki R, Hirakawa H, Nagamune T.

Biotechnol J. 2014 Dec;9(12):1573-81. doi: 10.1002/biot.201400007. Epub 2014 Jul 21.

PMID:
24924478
38.

Visualization of the pH-dependent dynamic distribution of G2A in living cells.

Lan W, Yamaguchi S, Yamamoto T, Yamahira S, Tan M, Murakami N, Zhang J, Nakamura M, Nagamune T.

FASEB J. 2014 Sep;28(9):3965-74. doi: 10.1096/fj.14-252999. Epub 2014 Jun 2.

39.

AAG8 promotes carcinogenesis by activating STAT3.

Sun B, Kawahara M, Ehata S, Nagamune T.

Cell Signal. 2014 Sep;26(9):1863-9. doi: 10.1016/j.cellsig.2014.04.001. Epub 2014 Apr 12.

PMID:
24726897
40.

Modeling tandem AAG8-MEK inhibition in melanoma cells.

Sun B, Kawahara M, Nagamune T.

Cancer Med. 2014 Jun;3(3):710-8. doi: 10.1002/cam4.233. Epub 2014 Mar 14.

41.

Dynamic photochemical lipid micropatterning for manipulation of nonadherent mammalian cells.

Yamahira S, Takasaki Y, Yamaguchi S, Sumaru K, Kanamori T, Nagamune T.

Methods Cell Biol. 2014;120:131-44. doi: 10.1016/B978-0-12-417136-7.00008-2.

PMID:
24484661
42.

S-Fms signalobody enhances myeloid cell growth and migration.

Kawahara M, Hitomi A, Nagamune T.

Biotechnol J. 2014 Jul;9(7):954-61. doi: 10.1002/biot.201300346. Epub 2014 Jan 22.

PMID:
24376185
43.

Antigen-responsive regulation of Cell motility and migration via the signalobodies based on c-Fms and c-Mpl.

Kawahara M, Hitomi A, Nagamune T.

Biotechnol Prog. 2014 Mar-Apr;30(2):411-7. doi: 10.1002/btpr.1861. Epub 2014 Jan 13.

PMID:
24375974
44.

A novel platform for antibody library selection in mammalian cells based on a growth signalobody.

Yoshida R, Kawahara M, Nagamune T.

Biotechnol Bioeng. 2014 Jun;111(6):1170-9. doi: 10.1002/bit.25173. Epub 2013 Dec 27.

PMID:
24338724
45.

Quantitative control of intracellular signaling activity through chimeric receptors incorporating multiple identical tyrosine motifs.

Saka K, Kawahara M, Nagamune T.

Biotechnol Bioeng. 2014 May;111(5):948-55. doi: 10.1002/bit.25151. Epub 2013 Nov 27.

PMID:
24222636
46.

Top-down motif engineering of a cytokine receptor for directing ex vivo expansion of hematopoietic stem cells.

Saka K, Kawahara M, Teng J, Otsu M, Nakauchi H, Nagamune T.

J Biotechnol. 2013 Dec;168(4):659-65. doi: 10.1016/j.jbiotec.2013.09.012. Epub 2013 Sep 23.

PMID:
24070902
47.

Fine tuning of spatial arrangement of enzymes in a PCNA-mediated multienzyme complex using a rigid poly-L-proline linker.

Haga T, Hirakawa H, Nagamune T.

PLoS One. 2013 Sep 5;8(9):e75114. doi: 10.1371/journal.pone.0075114. eCollection 2013.

48.

Construction of unnatural heterodimeric receptors based on IL-2 and IL-6 receptor subunits.

Ogawa K, Kawahara M, Nagamune T.

Biotechnol Prog. 2013 Nov-Dec;29(6):1512-8. doi: 10.1002/btpr.1785. Epub 2013 Aug 29.

PMID:
23926072
49.

Transfer printing of transfected cell microarrays from poly(ethylene glycol)-oleyl surfaces onto biological hydrogels.

Yamaguchi S, Komiya S, Matsunuma E, Yamahira S, Kihara T, Miyake J, Nagamune T.

Biotechnol Bioeng. 2013 Dec;110(12):3269-74. doi: 10.1002/bit.25010. Epub 2013 Aug 22.

PMID:
23893595
50.

Cell fate conversion by conditionally switching the signal-transducing domain of signalobodies.

Tone Y, Kawahara M, Hayashi J, Nagamune T.

Biotechnol Bioeng. 2013 Dec;110(12):3219-26. doi: 10.1002/bit.24985. Epub 2013 Aug 9.

PMID:
23794462

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