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Use of the CRISPR-Cas9 System in Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes.

Bosch JA, Knight S, Kanca O, Zirin J, Yang-Zhou D, Hu Y, Rodiger J, Amador G, Bellen HJ, Perrimon N, Mohr SE.

Curr Protoc Mol Biol. 2020 Mar;130(1):e112. doi: 10.1002/cpmb.112.


SNP-CRISPR: A Web Tool for SNP-Specific Genome Editing.

Chen CL, Rodiger J, Chung V, Viswanatha R, Mohr SE, Hu Y, Perrimon N.

G3 (Bethesda). 2020 Feb 6;10(2):489-494. doi: 10.1534/g3.119.400904.


Pooled CRISPR Screens in Drosophila Cells.

Viswanatha R, Brathwaite R, Hu Y, Li Z, Rodiger J, Merckaert P, Chung V, Mohr SE, Perrimon N.

Curr Protoc Mol Biol. 2019 Dec;129(1):e111. doi: 10.1002/cpmb.111.


An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms.

Kanca O, Zirin J, Garcia-Marques J, Knight SM, Yang-Zhou D, Amador G, Chung H, Zuo Z, Ma L, He Y, Lin WW, Fang Y, Ge M, Yamamoto S, Schulze KL, Hu Y, Spradling AC, Mohr SE, Perrimon N, Bellen HJ.

Elife. 2019 Nov 1;8. pii: e51539. doi: 10.7554/eLife.51539.


Drosophila melanogaster: a simple system for understanding complexity.

Mohr SE, Perrimon N.

Dis Model Mech. 2019 Sep 27;12(10). pii: dmm041871. doi: 10.1242/dmm.041871.


Methionine metabolism and methyltransferases in the regulation of aging and lifespan extension across species.

Parkhitko AA, Jouandin P, Mohr SE, Perrimon N.

Aging Cell. 2019 Dec;18(6):e13034. doi: 10.1111/acel.13034. Epub 2019 Aug 28. Review.


Editorial: Metal Biology Takes Flight: The Study of Metal Homeostasis and Detoxification in Insects.

Mohr SE, Killilea DW.

Front Genet. 2018 Jun 19;9:221. doi: 10.3389/fgene.2018.00221. eCollection 2018. No abstract available.


A gene-specific T2A-GAL4 library for Drosophila.

Lee PT, Zirin J, Kanca O, Lin WW, Schulze KL, Li-Kroeger D, Tao R, Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N, Bellen HJ.

Elife. 2018 Mar 22;7. pii: e35574. doi: 10.7554/eLife.35574.


Zinc Detoxification: A Functional Genomics and Transcriptomics Analysis in Drosophila melanogaster Cultured Cells.

Mohr SE, Rudd K, Hu Y, Song WR, Gilly Q, Buckner M, Housden BE, Kelley C, Zirin J, Tao R, Amador G, Sierzputowska K, Comjean A, Perrimon N.

G3 (Bethesda). 2018 Feb 2;8(2):631-641. doi: 10.1534/g3.117.300447.


Molecular Interaction Search Tool (MIST): an integrated resource for mining gene and protein interaction data.

Hu Y, Vinayagam A, Nand A, Comjean A, Chung V, Hao T, Mohr SE, Perrimon N.

Nucleic Acids Res. 2018 Jan 4;46(D1):D567-D574. doi: 10.1093/nar/gkx1116.


Accessing the Phenotype Gap: Enabling Systematic Investigation of Paralog Functional Complexity with CRISPR.

Ewen-Campen B, Mohr SE, Hu Y, Perrimon N.

Dev Cell. 2017 Oct 9;43(1):6-9. doi: 10.1016/j.devcel.2017.09.020. Review.


Optimized strategy for in vivo Cas9-activation in Drosophila.

Ewen-Campen B, Yang-Zhou D, Fernandes VR, González DP, Liu LP, Tao R, Ren X, Sun J, Hu Y, Zirin J, Mohr SE, Ni JQ, Perrimon N.

Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):9409-9414. doi: 10.1073/pnas.1707635114. Epub 2017 Aug 14.


Gene2Function: An Integrated Online Resource for Gene Function Discovery.

Hu Y, Comjean A, Mohr SE; FlyBase Consortium, Perrimon N.

G3 (Bethesda). 2017 Aug 7;7(8):2855-2858. doi: 10.1534/g3.117.043885.


MARRVEL: Integration of Human and Model Organism Genetic Resources to Facilitate Functional Annotation of the Human Genome.

Wang J, Al-Ouran R, Hu Y, Kim SY, Wan YW, Wangler MF, Yamamoto S, Chao HT, Comjean A, Mohr SE; UDN, Perrimon N, Liu Z, Bellen HJ.

Am J Hum Genet. 2017 Jun 1;100(6):843-853. doi: 10.1016/j.ajhg.2017.04.010. Epub 2017 May 11.


The Drosophila Gene Expression Tool (DGET) for expression analyses.

Hu Y, Comjean A, Perrimon N, Mohr SE.

BMC Bioinformatics. 2017 Feb 10;18(1):98. doi: 10.1186/s12859-017-1509-z.

16. database of the Drosophila RNAi screening center and transgenic RNAi project: 2017 update.

Hu Y, Comjean A, Roesel C, Vinayagam A, Flockhart I, Zirin J, Perkins L, Perrimon N, Mohr SE.

Nucleic Acids Res. 2017 Jan 4;45(D1):D672-D678. doi: 10.1093/nar/gkw977. Epub 2016 Oct 23.


Loss-of-function genetic tools for animal models: cross-species and cross-platform differences.

Housden BE, Muhar M, Gemberling M, Gersbach CA, Stainier DY, Seydoux G, Mohr SE, Zuber J, Perrimon N.

Nat Rev Genet. 2017 Jan;18(1):24-40. doi: 10.1038/nrg.2016.118. Epub 2016 Oct 31. Review.


An Integrative Analysis of the InR/PI3K/Akt Network Identifies the Dynamic Response to Insulin Signaling.

Vinayagam A, Kulkarni MM, Sopko R, Sun X, Hu Y, Nand A, Villalta C, Moghimi A, Yang X, Mohr SE, Hong P, Asara JM, Perrimon N.

Cell Rep. 2016 Sep 13;16(11):3062-3074. doi: 10.1016/j.celrep.2016.08.029.


CRISPR guide RNA design for research applications.

Mohr SE, Hu Y, Ewen-Campen B, Housden BE, Viswanatha R, Perrimon N.

FEBS J. 2016 Sep;283(17):3232-8. doi: 10.1111/febs.13777. Epub 2016 Jun 22. Review.


Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi.

Housden BE, Valvezan AJ, Kelley C, Sopko R, Hu Y, Roesel C, Lin S, Buckner M, Tao R, Yilmazel B, Mohr SE, Manning BD, Perrimon N.

Sci Signal. 2015 Sep 8;8(393):rs9. doi: 10.1126/scisignal.aab3729.


The Transgenic RNAi Project at Harvard Medical School: Resources and Validation.

Perkins LA, Holderbaum L, Tao R, Hu Y, Sopko R, McCall K, Yang-Zhou D, Flockhart I, Binari R, Shim HS, Miller A, Housden A, Foos M, Randkelv S, Kelley C, Namgyal P, Villalta C, Liu LP, Jiang X, Huan-Huan Q, Wang X, Fujiyama A, Toyoda A, Ayers K, Blum A, Czech B, Neumuller R, Yan D, Cavallaro A, Hibbard K, Hall D, Cooley L, Hannon GJ, Lehmann R, Parks A, Mohr SE, Ueda R, Kondo S, Ni JQ, Perrimon N.

Genetics. 2015 Nov;201(3):843-52. doi: 10.1534/genetics.115.180208. Epub 2015 Aug 28.


Reagent and Data Resources for Investigation of RNA Binding Protein Functions in Drosophila melanogaster Cultured Cells.

Mohr SE, Hu Y, Rudd K, Buckner M, Gilly Q, Foster B, Sierzputowska K, Comjean A, Ye B, Perrimon N.

G3 (Bethesda). 2015 Jul 21;5(9):1919-24. doi: 10.1534/g3.115.019364.


GLAD: an Online Database of Gene List Annotation for Drosophila.

Hu Y, Comjean A, Perkins LA, Perrimon N, Mohr SE.

J Genomics. 2015 Jul 1;3:75-81. doi: 10.7150/jgen.12863. eCollection 2015.


The potential role of temporal dynamics in approach biases: delay-dependence of a general approach bias in an alcohol approach-avoidance task.

Gladwin TE, Mohr SE, Wiers RW.

Front Psychol. 2014 Dec 1;5:1398. doi: 10.3389/fpsyg.2014.01398. eCollection 2014.


RNAi screening comes of age: improved techniques and complementary approaches.

Mohr SE, Smith JA, Shamu CE, Neumüller RA, Perrimon N.

Nat Rev Mol Cell Biol. 2014 Sep;15(9):591-600. doi: 10.1038/nrm3860. Review.


Online GESS: prediction of miRNA-like off-target effects in large-scale RNAi screen data by seed region analysis.

Yilmazel B, Hu Y, Sigoillot F, Smith JA, Shamu CE, Perrimon N, Mohr SE.

BMC Bioinformatics. 2014 Jun 17;15:192. doi: 10.1186/1471-2105-15-192.


Approach, avoidance, and affect: a meta-analysis of approach-avoidance tendencies in manual reaction time tasks.

Phaf RH, Mohr SE, Rotteveel M, Wicherts JM.

Front Psychol. 2014 May 8;5:378. doi: 10.3389/fpsyg.2014.00378. eCollection 2014.


Resources for functional genomics studies in Drosophila melanogaster.

Mohr SE, Hu Y, Kim K, Housden BE, Perrimon N.

Genetics. 2014 May;197(1):1-18. doi: 10.1534/genetics.113.154344. Epub 2014 Mar 20. Review.


RNAi screening in Drosophila cells and in vivo.

Mohr SE.

Methods. 2014 Jun 15;68(1):82-8. doi: 10.1016/j.ymeth.2014.02.018. Epub 2014 Feb 24. Review.


Integrating protein-protein interaction networks with phenotypes reveals signs of interactions.

Vinayagam A, Zirin J, Roesel C, Hu Y, Yilmazel B, Samsonova AA, Neumüller RA, Mohr SE, Perrimon N.

Nat Methods. 2014 Jan;11(1):94-9. doi: 10.1038/nmeth.2733. Epub 2013 Nov 17.


Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9.

Ren X, Sun J, Housden BE, Hu Y, Roesel C, Lin S, Liu LP, Yang Z, Mao D, Sun L, Wu Q, Ji JY, Xi J, Mohr SE, Xu J, Perrimon N, Ni JQ.

Proc Natl Acad Sci U S A. 2013 Nov 19;110(47):19012-7. doi: 10.1073/pnas.1318481110. Epub 2013 Nov 4.


FlyPrimerBank: an online database for Drosophila melanogaster gene expression analysis and knockdown evaluation of RNAi reagents.

Hu Y, Sopko R, Foos M, Kelley C, Flockhart I, Ammeux N, Wang X, Perkins L, Perrimon N, Mohr SE.

G3 (Bethesda). 2013 Sep 4;3(9):1607-16. doi: 10.1534/g3.113.007021.


UP-TORR: online tool for accurate and Up-to-Date annotation of RNAi Reagents.

Hu Y, Roesel C, Flockhart I, Perkins L, Perrimon N, Mohr SE.

Genetics. 2013 Sep;195(1):37-45. doi: 10.1534/genetics.113.151340. Epub 2013 Jun 21.


Genetic determinants of phosphate response in Drosophila.

Bergwitz C, Wee MJ, Sinha S, Huang J, DeRobertis C, Mensah LB, Cohen J, Friedman A, Kulkarni M, Hu Y, Vinayagam A, Schnall-Levin M, Berger B, Perkins LA, Mohr SE, Perrimon N.

PLoS One. 2013;8(3):e56753. doi: 10.1371/journal.pone.0056753. Epub 2013 Mar 8.


Protein complex-based analysis framework for high-throughput data sets.

Vinayagam A, Hu Y, Kulkarni M, Roesel C, Sopko R, Mohr SE, Perrimon N.

Sci Signal. 2013 Feb 26;6(264):rs5. doi: 10.1126/scisignal.2003629.


Stringent analysis of gene function and protein-protein interactions using fluorescently tagged genes.

Neumüller RA, Wirtz-Peitz F, Lee S, Kwon Y, Buckner M, Hoskins RA, Venken KJ, Bellen HJ, Mohr SE, Perrimon N.

Genetics. 2012 Mar;190(3):931-40. doi: 10.1534/genetics.111.136465. Epub 2011 Dec 14.

37. database of the Drosophila RNAi screening center: 2012 update.

Flockhart IT, Booker M, Hu Y, McElvany B, Gilly Q, Mathey-Prevot B, Perrimon N, Mohr SE.

Nucleic Acids Res. 2012 Jan;40(Database issue):D715-9. doi: 10.1093/nar/gkr953. Epub 2011 Nov 8.


RNAi screening: new approaches, understandings, and organisms.

Mohr SE, Perrimon N.

Wiley Interdiscip Rev RNA. 2012 Mar-Apr;3(2):145-58. doi: 10.1002/wrna.110. Epub 2011 Sep 22. Review.


An integrative approach to ortholog prediction for disease-focused and other functional studies.

Hu Y, Flockhart I, Vinayagam A, Bergwitz C, Berger B, Perrimon N, Mohr SE.

BMC Bioinformatics. 2011 Aug 31;12:357. doi: 10.1186/1471-2105-12-357.


False negative rates in Drosophila cell-based RNAi screens: a case study.

Booker M, Samsonova AA, Kwon Y, Flockhart I, Mohr SE, Perrimon N.

BMC Genomics. 2011 Jan 20;12:50. doi: 10.1186/1471-2164-12-50.


Protein Structure Initiative Material Repository: an open shared public resource of structural genomics plasmids for the biological community.

Cormier CY, Mohr SE, Zuo D, Hu Y, Rolfs A, Kramer J, Taycher E, Kelley F, Fiacco M, Turnbull G, LaBaer J.

Nucleic Acids Res. 2010 Jan;38(Database issue):D743-9. doi: 10.1093/nar/gkp999. Epub 2009 Nov 11.


Large-scale functional annotation and expanded implementations of the P{wHy} hybrid transposon in the Drosophila melanogaster genome.

Myrick KV, Huet F, Mohr SE, Alvarez-García I, Lu JT, Smith MA, Crosby MA, Gelbart WM.

Genetics. 2009 Jul;182(3):653-60. doi: 10.1534/genetics.109.103762. Epub 2009 Apr 27.


Production and sequence validation of a complete full length ORF collection for the pathogenic bacterium Vibrio cholerae.

Rolfs A, Montor WR, Yoon SS, Hu Y, Bhullar B, Kelley F, McCarron S, Jepson DA, Shen B, Taycher E, Mohr SE, Zuo D, Williamson J, Mekalanos J, Labaer J.

Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4364-9. doi: 10.1073/pnas.0712049105. Epub 2008 Mar 12.


A full-genomic sequence-verified protein-coding gene collection for Francisella tularensis.

Murthy T, Rolfs A, Hu Y, Shi Z, Raphael J, Moreira D, Kelley F, McCarron S, Jepson D, Taycher E, Zuo D, Mohr SE, Fernandez M, Brizuela L, LaBaer J.

PLoS One. 2007 Jun 27;2(6):e577.


A novel approach to sequence validating protein expression clones with automated decision making.

Taycher E, Rolfs A, Hu Y, Zuo D, Mohr SE, Williamson J, Labaer J.

BMC Bioinformatics. 2007 Jun 13;8:198.


PlasmID: a centralized repository for plasmid clone information and distribution.

Zuo D, Mohr SE, Hu Y, Taycher E, Rolfs A, Kramer J, Williamson J, LaBaer J.

Nucleic Acids Res. 2007 Jan;35(Database issue):D680-4. Epub 2006 Nov 28.


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