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Items: 1 to 50 of 170


RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium.

Cañadas IC, Groothuis D, Zygouropoulou M, Rodrigues R, Minton NP.

ACS Synth Biol. 2019 Jun 7. doi: 10.1021/acssynbio.9b00075. [Epub ahead of print]


Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system.

Ingle P, Groothuis D, Rowe P, Huang H, Cockayne A, Kuehne SA, Jiang W, Gu Y, Humphreys CM, Minton NP.

Sci Rep. 2019 May 31;9(1):8123. doi: 10.1038/s41598-019-44458-y.


Design, cloning and characterization of transcription factor-based inducible gene expression systems.

Hanko EKR, Minton NP, Malys N.

Methods Enzymol. 2019;621:153-169. doi: 10.1016/bs.mie.2019.02.018. Epub 2019 Mar 6.


Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum.

Annan FJ, Al-Sinawi B, Humphreys CM, Norman R, Winzer K, Köpke M, Simpson SD, Minton NP, Henstra AM.

Appl Microbiol Biotechnol. 2019 Jun;103(11):4633-4648. doi: 10.1007/s00253-019-09763-6. Epub 2019 Apr 10.


Homologous overexpression of hydrogenase and glycerol dehydrogenase in Clostridium pasteurianum to enhance hydrogen production from crude glycerol.

Sarma S, Ortega D, Minton NP, Dubey VK, Moholkar VS.

Bioresour Technol. 2019 Jul;284:168-177. doi: 10.1016/j.biortech.2019.03.074. Epub 2019 Mar 16.


CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii.

Li Q, Seys FM, Minton NP, Yang J, Jiang Y, Jiang W, Yang S.

Biotechnol Bioeng. 2019 Jun;116(6):1475-1483. doi: 10.1002/bit.26949. Epub 2019 Feb 21.


Regulation of lactate metabolism in the acetogenic bacterium Acetobacterium woodii.

Schoelmerich MC, Katsyv A, Sung W, Mijic V, Wiechmann A, Kottenhahn P, Baker J, Minton NP, Müller V.

Environ Microbiol. 2018 Dec;20(12):4587-4595. doi: 10.1111/1462-2920.14412. Epub 2018 Oct 24.


The Rnf Complex Is an Energy-Coupled Transhydrogenase Essential To Reversibly Link Cellular NADH and Ferredoxin Pools in the Acetogen Acetobacterium woodii.

Westphal L, Wiechmann A, Baker J, Minton NP, Müller V.

J Bacteriol. 2018 Oct 10;200(21). pii: e00357-18. doi: 10.1128/JB.00357-18. Print 2018 Nov 1.


Functional Genetic Elements for Controlling Gene Expression in Cupriavidus necator H16.

Alagesan S, Hanko EKR, Malys N, Ehsaan M, Winzer K, Minton NP.

Appl Environ Microbiol. 2018 Sep 17;84(19). pii: e00878-18. doi: 10.1128/AEM.00878-18. Print 2018 Oct 1.


Effect of antibiotic treatment on the formation of non-spore Clostridium difficile persister-like cells.

Álvarez R, Inostroza O, Garavaglia M, Minton NP, Paredes-Sabja D, Gil F.

J Antimicrob Chemother. 2018 Sep 1;73(9):2396-2399. doi: 10.1093/jac/dky186.


Progress towards platform chemical production using Clostridium autoethanogenum.

Norman ROJ, Millat T, Winzer K, Minton NP, Hodgman C.

Biochem Soc Trans. 2018 Jun 19;46(3):523-535. doi: 10.1042/BST20170259. Epub 2018 Apr 17. Review.


The Butanol Producing Microbe Clostridium beijerinckii NCIMB 14988 Manipulated Using Forward and Reverse Genetic Tools.

Little GT, Willson BJ, Heap JT, Winzer K, Minton NP.

Biotechnol J. 2018 Nov;13(11):e1700711. doi: 10.1002/biot.201700711. Epub 2018 Apr 29.


A Transcription Factor-Based Biosensor for Detection of Itaconic Acid.

Hanko EKR, Minton NP, Malys N.

ACS Synth Biol. 2018 May 18;7(5):1436-1446. doi: 10.1021/acssynbio.8b00057. Epub 2018 Apr 17.


Quantitative Isotope-Dilution High-Resolution-Mass-Spectrometry Analysis of Multiple Intracellular Metabolites in Clostridium autoethanogenum with Uniformly 13C-Labeled Standards Derived from Spirulina.

Schatschneider S, Abdelrazig S, Safo L, Henstra AM, Millat T, Kim DH, Winzer K, Minton NP, Barrett DA.

Anal Chem. 2018 Apr 3;90(7):4470-4477. doi: 10.1021/acs.analchem.7b04758. Epub 2018 Mar 21.


Advances in metabolic engineering in the microbial production of fuels and chemicals from C1 gas.

Humphreys CM, Minton NP.

Curr Opin Biotechnol. 2018 Apr;50:174-181. doi: 10.1016/j.copbio.2017.12.023. Epub 2018 Feb 3. Review.


Characterization of the impact of rpoB mutations on the in vitro and in vivo competitive fitness of Clostridium difficile and susceptibility to fidaxomicin.

Kuehne SA, Dempster AW, Collery MM, Joshi N, Jowett J, Kelly ML, Cave R, Longshaw CM, Minton NP.

J Antimicrob Chemother. 2018 Apr 1;73(4):973-980. doi: 10.1093/jac/dkx486.


13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16.

Alagesan S, Minton NP, Malys N.

Metabolomics. 2018;14(1):9. doi: 10.1007/s11306-017-1302-z. Epub 2017 Dec 4.


Inactivation of the dnaK gene in Clostridium difficile 630 Δerm yields a temperature-sensitive phenotype and increases biofilm-forming ability.

Jain S, Smyth D, O'Hagan BMG, Heap JT, McMullan G, Minton NP, Ternan NG.

Sci Rep. 2017 Dec 13;7(1):17522. doi: 10.1038/s41598-017-17583-9.


Applying asymptotic methods to synthetic biology: Modelling the reaction kinetics of the mevalonate pathway.

Dalwadi MP, Garavaglia M, Webb JP, King JR, Minton NP.

J Theor Biol. 2018 Feb 14;439:39-49. doi: 10.1016/j.jtbi.2017.11.022. Epub 2017 Dec 2.


Multi-timescale analysis of a metabolic network in synthetic biology: a kinetic model for 3-hydroxypropionic acid production via beta-alanine.

Dalwadi MP, King JR, Minton NP.

J Math Biol. 2018 Jul;77(1):165-199. doi: 10.1007/s00285-017-1189-3. Epub 2017 Nov 20.


Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants.

Grosse-Honebrink A, Schwarz KM, Wang H, Minton NP, Zhang Y.

Anaerobe. 2017 Dec;48:203-205. doi: 10.1016/j.anaerobe.2017.09.001. Epub 2017 Sep 9.


Syngas Biorefinery and Syngas Utilization.

De Tissera S, Köpke M, Simpson SD, Humphreys C, Minton NP, Dürre P.

Adv Biochem Eng Biotechnol. 2019;166:247-280. doi: 10.1007/10_2017_5.


Microbial solvent formation revisited by comparative genome analysis.

Poehlein A, Solano JDM, Flitsch SK, Krabben P, Winzer K, Reid SJ, Jones DT, Green E, Minton NP, Daniel R, Dürre P.

Biotechnol Biofuels. 2017 Mar 9;10:58. doi: 10.1186/s13068-017-0742-z. eCollection 2017.


Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum.

Schwarz KM, Grosse-Honebrink A, Derecka K, Rotta C, Zhang Y, Minton NP.

Metab Eng. 2017 Mar;40:124-137. doi: 10.1016/j.ymben.2017.01.009. Epub 2017 Jan 22.


Metabolic engineering of Clostridium autoethanogenum for selective alcohol production.

Liew F, Henstra AM, Kӧpke M, Winzer K, Simpson SD, Minton NP.

Metab Eng. 2017 Mar;40:104-114. doi: 10.1016/j.ymben.2017.01.007. Epub 2017 Jan 19.


Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels.

Bilverstone TW, Kinsmore NL, Minton NP, Kuehne SA.

Anaerobe. 2017 Apr;44:51-54. doi: 10.1016/j.anaerobe.2017.01.009. Epub 2017 Jan 17.


Functional Intestinal Bile Acid 7α-Dehydroxylation by Clostridium scindens Associated with Protection from Clostridium difficile Infection in a Gnotobiotic Mouse Model.

Studer N, Desharnais L, Beutler M, Brugiroux S, Terrazos MA, Menin L, Schürch CM, McCoy KD, Kuehne SA, Minton NP, Stecher B, Bernier-Latmani R, Hapfelmeier S.

Front Cell Infect Microbiol. 2016 Dec 20;6:191. doi: 10.3389/fcimb.2016.00191. eCollection 2016.


Development and implementation of rapid metabolic engineering tools for chemical and fuel production in Geobacillus thermoglucosidasius NCIMB 11955.

Sheng L, Kovács K, Winzer K, Zhang Y, Minton NP.

Biotechnol Biofuels. 2017 Jan 3;10:5. doi: 10.1186/s13068-016-0692-x. eCollection 2017.


A genetic assay for gene essentiality in Clostridium.

Walker DJF, Heap JT, Winzer K, Minton NP.

Anaerobe. 2016 Dec;42:40-43. doi: 10.1016/j.anaerobe.2016.07.007. Epub 2016 Jul 31.


Enhanced solvent production by metabolic engineering of a twin-clostridial consortium.

Wen Z, Minton NP, Zhang Y, Li Q, Liu J, Jiang Y, Yang S.

Metab Eng. 2017 Jan;39:38-48. doi: 10.1016/j.ymben.2016.10.013. Epub 2016 Oct 27.


Complete Genome Sequence of Geobacillus thermoglucosidasius NCIMB 11955, the Progenitor of a Bioethanol Production Strain.

Sheng L, Zhang Y, Minton NP.

Genome Announc. 2016 Sep 29;4(5). pii: e01065-16. doi: 10.1128/genomeA.01065-16.


What's a SNP between friends: The influence of single nucleotide polymorphisms on virulence and phenotypes of Clostridium difficile strain 630 and derivatives.

Collery MM, Kuehne SA, McBride SM, Kelly ML, Monot M, Cockayne A, Dupuy B, Minton NP.

Virulence. 2017 Aug 18;8(6):767-781. doi: 10.1080/21505594.2016.1237333. Epub 2016 Sep 21.


The binary toxin CDT enhances Clostridium difficile virulence by suppressing protective colonic eosinophilia.

Cowardin CA, Buonomo EL, Saleh MM, Wilson MG, Burgess SL, Kuehne SA, Schwan C, Eichhoff AM, Koch-Nolte F, Lyras D, Aktories K, Minton NP, Petri WA Jr.

Nat Microbiol. 2016 Jul 11;1(8):16108. doi: 10.1038/nmicrobiol.2016.108.


Coinfection and Emergence of Rifamycin Resistance during a Recurrent Clostridium difficile Infection.

Stevenson EC, Major GA, Spiller RC, Kuehne SA, Minton NP.

J Clin Microbiol. 2016 Nov;54(11):2689-2694. Epub 2016 Aug 24.


Clostridium difficile Genome Editing Using pyrE Alleles.

Ehsaan M, Kuehne SA, Minton NP.

Methods Mol Biol. 2016;1476:35-52. doi: 10.1007/978-1-4939-6361-4_4.


Advancing Clostridia to Clinical Trial: Past Lessons and Recent Progress.

Mowday AM, Guise CP, Ackerley DF, Minton NP, Lambin P, Dubois LJ, Theys J, Smaill JB, Patterson AV.

Cancers (Basel). 2016 Jun 28;8(7). pii: E63. doi: 10.3390/cancers8070063. Review.


SBRC-Nottingham: sustainable routes to platform chemicals from C1 waste gases.

Burbidge A, Minton NP.

Biochem Soc Trans. 2016 Jun 15;44(3):684-6. doi: 10.1042/BST20160010.


CRISPR/Cas9-Based Efficient Genome Editing in Clostridium ljungdahlii, an Autotrophic Gas-Fermenting Bacterium.

Huang H, Chai C, Li N, Rowe P, Minton NP, Yang S, Jiang W, Gu Y.

ACS Synth Biol. 2016 Dec 16;5(12):1355-1361. Epub 2016 Jun 15.


A roadmap for gene system development in Clostridium.

Minton NP, Ehsaan M, Humphreys CM, Little GT, Baker J, Henstra AM, Liew F, Kelly ML, Sheng L, Schwarz K, Zhang Y.

Anaerobe. 2016 Oct;41:104-112. doi: 10.1016/j.anaerobe.2016.05.011. Epub 2016 May 24. Review.


Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824.

Willson BJ, Kovács K, Wilding-Steele T, Markus R, Winzer K, Minton NP.

Biotechnol Biofuels. 2016 May 23;9:109. doi: 10.1186/s13068-016-0526-x. eCollection 2016.


Insights into CO2 Fixation Pathway of Clostridium autoethanogenum by Targeted Mutagenesis.

Liew F, Henstra AM, Winzer K, Köpke M, Simpson SD, Minton NP.

MBio. 2016 May 24;7(3). pii: e00427-16. doi: 10.1128/mBio.00427-16.


CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii.

Li Q, Chen J, Minton NP, Zhang Y, Wen Z, Liu J, Yang H, Zeng Z, Ren X, Yang J, Gu Y, Jiang W, Jiang Y, Yang S.

Biotechnol J. 2016 Jul;11(7):961-72. doi: 10.1002/biot.201600053. Epub 2016 Jun 13.


Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum.

Zhang Y, Xu S, Chai C, Yang S, Jiang W, Minton NP, Gu Y.

FEMS Microbiol Lett. 2016 Apr;363(8). pii: fnw065. doi: 10.1093/femsle/fnw065. Epub 2016 Mar 20.


Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection.

Kelly ML, Ng YK, Cartman ST, Collery MM, Cockayne A, Minton NP.

Anaerobe. 2016 Jun;39:51-3. doi: 10.1016/j.anaerobe.2016.02.011. Epub 2016 Mar 2.


Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824.

Ehsaan M, Kuit W, Zhang Y, Cartman ST, Heap JT, Winzer K, Minton NP.

Biotechnol Biofuels. 2016 Jan 4;9:4. doi: 10.1186/s13068-015-0410-0. eCollection 2016.


Clostridium difficile-mediated effects on human intestinal epithelia: Modelling host-pathogen interactions in a vertical diffusion chamber.

Jafari NV, Kuehne SA, Minton NP, Allan E, Bajaj-Elliott M.

Anaerobe. 2016 Feb;37:96-102. doi: 10.1016/j.anaerobe.2015.12.007. Epub 2015 Dec 19.


Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium.

Humphreys CM, McLean S, Schatschneider S, Millat T, Henstra AM, Annan FJ, Breitkopf R, Pander B, Piatek P, Rowe P, Wichlacz AT, Woods C, Norman R, Blom J, Goesman A, Hodgman C, Barrett D, Thomas NR, Winzer K, Minton NP.

BMC Genomics. 2015 Dec 21;16:1085. doi: 10.1186/s12864-015-2287-5.


The SOS Response Master Regulator LexA Is Associated with Sporulation, Motility and Biofilm Formation in Clostridium difficile.

Walter BM, Cartman ST, Minton NP, Butala M, Rupnik M.

PLoS One. 2015 Dec 18;10(12):e0144763. doi: 10.1371/journal.pone.0144763. eCollection 2015.


Clostridium difficile secreted Pro-Pro endopeptidase PPEP-1 (ZMP1/CD2830) modulates adhesion through cleavage of the collagen binding protein CD2831.

Hensbergen PJ, Klychnikov OI, Bakker D, Dragan I, Kelly ML, Minton NP, Corver J, Kuijper EJ, Drijfhout JW, van Leeuwen HC.

FEBS Lett. 2015 Dec 21;589(24 Pt B):3952-8. doi: 10.1016/j.febslet.2015.10.027. Epub 2015 Oct 29.

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