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Items: 9


Photoactivatable Reaction for Covalent Nanoscale Patterning of Multiple Proteins.

Zhou S, Metcalf KJ, Bugga P, Grant J, Mrksich M.

ACS Appl Mater Interfaces. 2018 Nov 28;10(47):40452-40459. doi: 10.1021/acsami.8b16736. Epub 2018 Nov 14.


Long-Range Energy Transfer in Protein Megamolecules.

Taylor EL, Metcalf KJ, Carlotti B, Lai CT, Modica JA, Schatz GC, Mrksich M, Goodson T 3rd.

J Am Chem Soc. 2018 Nov 21;140(46):15731-15743. doi: 10.1021/jacs.8b08208. Epub 2018 Nov 9.


An estimate is worth about a thousand experiments: using order-of-magnitude estimates to identify cellular engineering targets.

Metcalf KJ, Slininger Lee MF, Jakobson CM, Tullman-Ercek D.

Microb Cell Fact. 2018 Aug 30;17(1):135. doi: 10.1186/s12934-018-0979-7. Review.


Proteins adopt functionally active conformations after type III secretion.

Metcalf KJ, Bevington JL, Rosales SL, Burdette LA, Valdivia E, Tullman-Ercek D.

Microb Cell Fact. 2016 Dec 23;15(1):213. doi: 10.1186/s12934-016-0606-4.


Use of Transcriptional Control to Increase Secretion of Heterologous Proteins in T3S Systems.

Metcalf KJ, Tullman-Ercek D.

Methods Mol Biol. 2017;1531:71-79.


Pro-region engineering for improved yeast display and secretion of brain derived neurotrophic factor.

Burns ML, Malott TM, Metcalf KJ, Puguh A, Chan JR, Shusta EV.

Biotechnol J. 2016 Mar;11(3):425-36. doi: 10.1002/biot.201500360. Epub 2015 Dec 9.


Type III secretion as a generalizable strategy for the production of full-length biopolymer-forming proteins.

Azam A, Li C, Metcalf KJ, Tullman-Ercek D.

Biotechnol Bioeng. 2016 Nov;113(11):2313-20. doi: 10.1002/bit.25656. Epub 2015 Jun 30.


Using transcriptional control to increase titers of secreted heterologous proteins by the type III secretion system.

Metcalf KJ, Finnerty C, Azam A, Valdivia E, Tullman-Ercek D.

Appl Environ Microbiol. 2014 Oct;80(19):5927-34. doi: 10.1128/AEM.01330-14. Epub 2014 Jul 18.


Directed evolution of brain-derived neurotrophic factor for improved folding and expression in Saccharomyces cerevisiae.

Burns ML, Malott TM, Metcalf KJ, Hackel BJ, Chan JR, Shusta EV.

Appl Environ Microbiol. 2014 Sep;80(18):5732-42. doi: 10.1128/AEM.01466-14. Epub 2014 Jul 11.

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