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Items: 1 to 50 of 91

1.

The presence of an RNA:DNA hybrid that is prone to slippage promotes termination by T7 RNA polymerase.

Molodtsov V, Anikin M, McAllister WT.

J Mol Biol. 2014 Sep 9;426(18):3095-3107. doi: 10.1016/j.jmb.2014.06.012. Epub 2014 Jun 27.

2.

Yeast DEAD box protein Mss116p is a transcription elongation factor that modulates the activity of mitochondrial RNA polymerase.

Markov DA, Wojtas ID, Tessitore K, Henderson S, McAllister WT.

Mol Cell Biol. 2014 Jul;34(13):2360-9. doi: 10.1128/MCB.00160-14. Epub 2014 Apr 14.

3.

Histone variant H2A.Z inhibits transcription in reconstituted nucleosomes.

Thakar A, Gupta P, McAllister WT, Zlatanova J.

Biochemistry. 2010 May 18;49(19):4018-26. doi: 10.1021/bi1001618.

PMID:
20387858
4.

Multiple functions of yeast mitochondrial transcription factor Mtf1p during initiation.

Savkina M, Temiakov D, McAllister WT, Anikin M.

J Biol Chem. 2010 Feb 5;285(6):3957-64. doi: 10.1074/jbc.M109.051003. Epub 2009 Nov 17.

5.

In a head-on collision, two RNA polymerases approaching one another on the same DNA may pass by one another.

Ma N, McAllister WT.

J Mol Biol. 2009 Sep 4;391(5):808-12. doi: 10.1016/j.jmb.2009.06.060. Epub 2009 Jul 1.

PMID:
19576223
6.

Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purification.

Markov DA, Savkina M, Anikin M, Del Campo M, Ecker K, Lambowitz AM, De Gnore JP, McAllister WT.

Yeast. 2009 Aug;26(8):423-40. doi: 10.1002/yea.1672.

7.

Determination of the melting site of the DNA duplex in the active center of bacterial RNA-polymerase by fluorescence quenching technique.

Kashkina EA, Anikin MV, McAllister WT, Kochetkov N, Temyakov DE.

Dokl Biochem Biophys. 2007 Sep-Oct;416:285-9. No abstract available.

PMID:
18064834
8.

The transition to an elongation complex by T7 RNA polymerase is a multistep process.

Bandwar RP, Ma N, Emanuel SA, Anikin M, Vassylyev DG, Patel SS, McAllister WT.

J Biol Chem. 2007 Aug 3;282(31):22879-86. Epub 2007 Jun 4.

9.

Multisubunit RNA polymerases melt only a single DNA base pair downstream of the active site.

Kashkina E, Anikin M, Brueckner F, Lehmann E, Kochetkov SN, McAllister WT, Cramer P, Temiakov D.

J Biol Chem. 2007 Jul 27;282(30):21578-82. Epub 2007 May 25. Erratum in: J Biol Chem. 2009 Aug 14;284(33):22500.

10.

Template misalignment in multisubunit RNA polymerases and transcription fidelity.

Kashkina E, Anikin M, Brueckner F, Pomerantz RT, McAllister WT, Cramer P, Temiakov D.

Mol Cell. 2006 Oct 20;24(2):257-66.

11.

A mechanism of nucleotide misincorporation during transcription due to template-strand misalignment.

Pomerantz RT, Temiakov D, Anikin M, Vassylyev DG, McAllister WT.

Mol Cell. 2006 Oct 20;24(2):245-55.

12.

Single-molecule approaches reveal the idiosyncrasies of RNA polymerases.

Zlatanova J, McAllister WT, Borukhov S, Leuba SH.

Structure. 2006 Jun;14(6):953-66. Review.

13.

Probing conformational changes in T7 RNA polymerase during initiation and termination by using engineered disulfide linkages.

Ma K, Temiakov D, Anikin M, McAllister WT.

Proc Natl Acad Sci U S A. 2005 Dec 6;102(49):17612-7. Epub 2005 Nov 21.

14.

A tightly regulated molecular motor based upon T7 RNA polymerase.

Pomerantz RT, Ramjit R, Gueroui Z, Place C, Anikin M, Leuba S, Zlatanova J, McAllister WT.

Nano Lett. 2005 Sep;5(9):1698-703.

PMID:
16159208
15.

RNA displacement and resolution of the transcription bubble during transcription by T7 RNA polymerase.

Jiang M, Ma N, Vassylyev DG, McAllister WT.

Mol Cell. 2004 Sep 10;15(5):777-88.

16.

Structural basis for substrate selection by t7 RNA polymerase.

Temiakov D, Patlan V, Anikin M, McAllister WT, Yokoyama S, Vassylyev DG.

Cell. 2004 Feb 6;116(3):381-91.

17.

Probing the organization of transcription complexes using photoreactive 4-thio-substituted analogs of uracil and thymidine.

Temiakov D, Anikin M, Ma K, Jiang M, McAllister WT.

Methods Enzymol. 2003;371:133-43. No abstract available.

PMID:
14712696
18.

Crystallization and preliminary crystallographic analysis of T7 RNA polymerase elongation complex.

Temiakov D, Tahirov TH, Anikin M, McAllister WT, Vassylyev DG, Yokoyama S.

Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):185-7. Epub 2002 Dec 19.

PMID:
12499566
19.

Exposure of T7 RNA polymerase to the isolated binding region of the promoter allows transcription from a single-stranded template.

Kukarin A, Rong M, McAllister WT.

J Biol Chem. 2003 Jan 24;278(4):2419-24. Epub 2002 Nov 18.

20.

Structure of a T7 RNA polymerase elongation complex at 2.9 A resolution.

Tahirov TH, Temiakov D, Anikin M, Patlan V, McAllister WT, Vassylyev DG, Yokoyama S.

Nature. 2002 Nov 7;420(6911):43-50. Epub 2002 Oct 9.

PMID:
12422209
21.

Characterization of T7 RNA polymerase transcription complexes assembled on nucleic acid scaffolds.

Temiakov D, Anikin M, McAllister WT.

J Biol Chem. 2002 Dec 6;277(49):47035-43. Epub 2002 Sep 25.

22.

Major conformational changes occur during the transition from an initiation complex to an elongation complex by T7 RNA polymerase.

Ma K, Temiakov D, Jiang M, Anikin M, McAllister WT.

J Biol Chem. 2002 Nov 8;277(45):43206-15. Epub 2002 Aug 16.

25.

Characterization of halted T7 RNA polymerase elongation complexes reveals multiple factors that contribute to stability.

Mentesana PE, Chin-Bow ST, Sousa R, McAllister WT.

J Mol Biol. 2000 Oct 6;302(5):1049-62.

PMID:
11183774
26.

The specificity loop of T7 RNA polymerase interacts first with the promoter and then with the elongating transcript, suggesting a mechanism for promoter clearance.

Temiakov D, Mentesana PE, Ma K, Mustaev A, Borukhov S, McAllister WT.

Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14109-14.

27.
28.

Cloning and purification of bacteriophage K11 RNA polymerase.

Rong M, Castagna R, McAllister WT.

Biotechniques. 1999 Oct;27(4):690, 692, 694. No abstract available.

30.

Studies of contacts between T7 RNA polymerase and its promoter reveal features in common with multisubunit RNA polymerases.

Place C, Oddos J, Buc H, McAllister WT, Buckle M.

Biochemistry. 1999 Apr 20;38(16):4948-57.

PMID:
10213596
31.

Characterization of an unusual, sequence-specific termination signal for T7 RNA polymerase.

He B, Kukarin A, Temiakov D, Chin-Bow ST, Lyakhov DL, Rong M, Durbin RK, McAllister WT.

J Biol Chem. 1998 Jul 24;273(30):18802-11.

32.

Pausing and termination by bacteriophage T7 RNA polymerase.

Lyakhov DL, He B, Zhang X, Studier FW, Dunn JJ, McAllister WT.

J Mol Biol. 1998 Jul 10;280(2):201-13.

PMID:
9654445
33.

Template strand switching by T7 RNA polymerase.

Rong M, Durbin RK, McAllister WT.

J Biol Chem. 1998 Apr 24;273(17):10253-60.

34.

Promoter specificity determinants of T7 RNA polymerase.

Rong M, He B, McAllister WT, Durbin RK.

Proc Natl Acad Sci U S A. 1998 Jan 20;95(2):515-9.

35.

Mutant bacteriophage T7 RNA polymerases with altered termination properties.

Lyakhov DL, He B, Zhang X, Studier FW, Dunn JJ, McAllister WT.

J Mol Biol. 1997 May 30;269(1):28-40.

PMID:
9192998
36.

Rapid mutagenesis and purification of phage RNA polymerases.

He B, Rong M, Lyakhov D, Gartenstein H, Diaz G, Castagna R, McAllister WT, Durbin RK.

Protein Expr Purif. 1997 Feb;9(1):142-51.

PMID:
9116496
37.

A mutant T7 RNA polymerase that is defective in RNA binding and blocked in the early stages of transcription.

He B, Rong M, Durbin RK, McAllister WT.

J Mol Biol. 1997 Jan 24;265(3):275-88.

PMID:
9018042
38.

The stability of abortively cycling T7 RNA polymerase complexes depends upon template conformation.

Diaz GA, Rong M, McAllister WT, Durbin RK.

Biochemistry. 1996 Aug 20;35(33):10837-43.

PMID:
8718875
39.
40.

Characterization of two types of termination signal for bacteriophage T7 RNA polymerase.

Macdonald LE, Durbin RK, Dunn JJ, McAllister WT.

J Mol Biol. 1994 Apr 29;238(2):145-58.

PMID:
8158645
41.

The phage RNA polymerases are related to DNA polymerases and reverse transcriptases.

McAllister WT, Raskin CA.

Mol Microbiol. 1993 Oct;10(1):1-6. Review.

PMID:
7526118
42.

Termination and slippage by bacteriophage T7 RNA polymerase.

Macdonald LE, Zhou Y, McAllister WT.

J Mol Biol. 1993 Aug 20;232(4):1030-47.

PMID:
8371265
43.

T7 RNA polymerase mutants with altered promoter specificities.

Raskin CA, Diaz GA, McAllister WT.

Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3147-51.

44.

Hierarchy of base-pair preference in the binding domain of the bacteriophage T7 promoter.

Diaz GA, Raskin CA, McAllister WT.

J Mol Biol. 1993 Feb 20;229(4):805-11.

PMID:
8445647
45.

Structure and function of the bacteriophage T7 RNA polymerase (or, the virtues of simplicity).

McAllister WT.

Cell Mol Biol Res. 1993;39(4):385-91. Review.

PMID:
8312975
46.
47.

Characterization of bacteriophage T7 RNA polymerase by linker insertion mutagenesis.

Gross L, Chen WJ, McAllister WT.

J Mol Biol. 1992 Nov 20;228(2):488-505.

PMID:
1453459
48.

A modified kanamycin-resistance cassette to facilitate two-codon insertion mutagenesis.

Chen WJ, Gross L, Joho KE, McAllister WT.

Gene. 1992 Feb 1;111(1):143-4.

PMID:
1547951
49.

Specific contacts between the bacteriophage T3, T7, and SP6 RNA polymerases and their promoters.

Jorgensen ED, Durbin RK, Risman SS, McAllister WT.

J Biol Chem. 1991 Jan 5;266(1):645-51.

50.

Single crystals of a chimeric T7/T3 RNA polymerase with T3 promoter specificity and a nonprocessive T7 RNAP mutant.

Sousa R, Chung YJ, McAllister WT, Wang BC, Lafer EM.

J Biol Chem. 1990 Dec 15;265(35):21430-2.

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